Ebola Virus VP35 Interaction with Dynein LC8 Regulates Viral RNA Synthesis
Abstract
Ebola virus VP35 inhibits alpha/beta interferon production and functions as a viral polymerase cofactor. Previously, the 8-kDa cytoplasmic dynein light chain (LC8) was demonstrated to interact with VP35, but the functional consequences were unclear. Furthermore we demonstrate that the interaction is direct and of high affinity and that binding stabilizes the VP35 N-terminal oligomerization domain and enhances viral RNA synthesis. Mutational analysis demonstrates that VP35 interaction is required for the functional effects of LC8.
- Authors:
-
- Icahn School of Medicine at Mount Sinai, New York, NY (United States)
- Washington Univ. School of Medicine, St. Louis, MO (United States)
- Publication Date:
- Research Org.:
- Argonne National Laboratory (ANL), Argonne, IL (United States)
- Sponsoring Org.:
- National Institutes of Health (NIH)
- OSTI Identifier:
- 1351394
- Grant/Contract Number:
- R01AI107056; R01AI059536; U19AI109945; U19AI109664; U19 AI070489; R01AI081914; T32-CA09547-37
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Journal of Virology
- Additional Journal Information:
- Journal Volume: 89; Journal Issue: 9; Journal ID: ISSN 0022-538X
- Publisher:
- American Society for Microbiology
- Country of Publication:
- United States
- Language:
- ENGLISH
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES
Citation Formats
Luthra, Priya, Jordan, David S., Leung, Daisy W., Amarasinghe, Gaya K., and Basler, Christopher F. Ebola Virus VP35 Interaction with Dynein LC8 Regulates Viral RNA Synthesis. United States: N. p., 2015.
Web. doi:10.1128/JVI.03652-14.
Luthra, Priya, Jordan, David S., Leung, Daisy W., Amarasinghe, Gaya K., & Basler, Christopher F. Ebola Virus VP35 Interaction with Dynein LC8 Regulates Viral RNA Synthesis. United States. https://doi.org/10.1128/JVI.03652-14
Luthra, Priya, Jordan, David S., Leung, Daisy W., Amarasinghe, Gaya K., and Basler, Christopher F. Fri .
"Ebola Virus VP35 Interaction with Dynein LC8 Regulates Viral RNA Synthesis". United States. https://doi.org/10.1128/JVI.03652-14. https://www.osti.gov/servlets/purl/1351394.
@article{osti_1351394,
title = {Ebola Virus VP35 Interaction with Dynein LC8 Regulates Viral RNA Synthesis},
author = {Luthra, Priya and Jordan, David S. and Leung, Daisy W. and Amarasinghe, Gaya K. and Basler, Christopher F.},
abstractNote = {Ebola virus VP35 inhibits alpha/beta interferon production and functions as a viral polymerase cofactor. Previously, the 8-kDa cytoplasmic dynein light chain (LC8) was demonstrated to interact with VP35, but the functional consequences were unclear. Furthermore we demonstrate that the interaction is direct and of high affinity and that binding stabilizes the VP35 N-terminal oligomerization domain and enhances viral RNA synthesis. Mutational analysis demonstrates that VP35 interaction is required for the functional effects of LC8.},
doi = {10.1128/JVI.03652-14},
journal = {Journal of Virology},
number = 9,
volume = 89,
place = {United States},
year = {Fri Apr 03 00:00:00 EDT 2015},
month = {Fri Apr 03 00:00:00 EDT 2015}
}
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