Characterization of an activation-tagged mutant uncovers a role of GLABRA2 in anthocyanin biosynthesis in Arabidopsis
Abstract
In Arabidopsis, anthocyanin biosynthesis is controlled by a MYB-bHLH-WD40 (MBW) transcriptional activator complex. The MBW complex activates the transcription of late biosynthesis genes in the flavonoid pathway, leading to the production of anthocyanins. A similar MBW complex regulates epidermal cell fate by activating the transcription of GLABRA2 (GL2), a homeodomain transcription factor required for trichome formation in shoots and non-hair cell formation in roots. Here we provide experimental evidence to show that GL2 also plays a role in regulating anthocyanin biosynthesis in Arabidopsis. From an activation-tagged mutagenized population of Arabidopsis plants, we isolated a dominant, gain-of-function mutant with reduced anthocyanins. Molecular cloning revealed that this phenotype is caused by an elevated expression of GL2, thus the mutant was named gl2-1D. Consistent with the view that GL2 acts as a negative regulator of anthocyanin biosynthesis, gl2-1D seedlings accumulated less whereas gl2-3 seedlings accumulated more anthocyanins in response to sucrose. Gene expression analysis indicated that expression of late, but not early, biosynthesis genes in the flavonoid pathway was dramatically reduced in gl2-1D but elevated in gl2-3 mutants. Further analysis showed that expression of some MBW component genes involved in the regulation of late biosynthesis genes was reduced in gl2-1D but elevated inmore »
- Authors:
-
- Northeast Normal Univ., Jilin (China)
- China Agricultural Univ., Beijing (China)
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Publication Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE Laboratory Directed Research and Development (LDRD) Program
- OSTI Identifier:
- 1286888
- Grant/Contract Number:
- AC05-00OR22725
- Resource Type:
- Accepted Manuscript
- Journal Name:
- The Plant Journal
- Additional Journal Information:
- Journal Volume: 83; Journal Issue: 2; Journal ID: ISSN 0960-7412
- Publisher:
- Society for Experimental Biology
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Wang, Xiaoyu, Wang, Xianling, Hu, Qingnan, Dai, Xuemei, Tian, Hainan, Zheng, Kaijie, Wang, Xiaoping, Mao, Tonglin, Chen, Jin -Gui, and Wang, Shucai. Characterization of an activation-tagged mutant uncovers a role of GLABRA2 in anthocyanin biosynthesis in Arabidopsis. United States: N. p., 2015.
Web. doi:10.1111/tpj.12887.
Wang, Xiaoyu, Wang, Xianling, Hu, Qingnan, Dai, Xuemei, Tian, Hainan, Zheng, Kaijie, Wang, Xiaoping, Mao, Tonglin, Chen, Jin -Gui, & Wang, Shucai. Characterization of an activation-tagged mutant uncovers a role of GLABRA2 in anthocyanin biosynthesis in Arabidopsis. United States. https://doi.org/10.1111/tpj.12887
Wang, Xiaoyu, Wang, Xianling, Hu, Qingnan, Dai, Xuemei, Tian, Hainan, Zheng, Kaijie, Wang, Xiaoping, Mao, Tonglin, Chen, Jin -Gui, and Wang, Shucai. Wed .
"Characterization of an activation-tagged mutant uncovers a role of GLABRA2 in anthocyanin biosynthesis in Arabidopsis". United States. https://doi.org/10.1111/tpj.12887. https://www.osti.gov/servlets/purl/1286888.
@article{osti_1286888,
title = {Characterization of an activation-tagged mutant uncovers a role of GLABRA2 in anthocyanin biosynthesis in Arabidopsis},
author = {Wang, Xiaoyu and Wang, Xianling and Hu, Qingnan and Dai, Xuemei and Tian, Hainan and Zheng, Kaijie and Wang, Xiaoping and Mao, Tonglin and Chen, Jin -Gui and Wang, Shucai},
abstractNote = {In Arabidopsis, anthocyanin biosynthesis is controlled by a MYB-bHLH-WD40 (MBW) transcriptional activator complex. The MBW complex activates the transcription of late biosynthesis genes in the flavonoid pathway, leading to the production of anthocyanins. A similar MBW complex regulates epidermal cell fate by activating the transcription of GLABRA2 (GL2), a homeodomain transcription factor required for trichome formation in shoots and non-hair cell formation in roots. Here we provide experimental evidence to show that GL2 also plays a role in regulating anthocyanin biosynthesis in Arabidopsis. From an activation-tagged mutagenized population of Arabidopsis plants, we isolated a dominant, gain-of-function mutant with reduced anthocyanins. Molecular cloning revealed that this phenotype is caused by an elevated expression of GL2, thus the mutant was named gl2-1D. Consistent with the view that GL2 acts as a negative regulator of anthocyanin biosynthesis, gl2-1D seedlings accumulated less whereas gl2-3 seedlings accumulated more anthocyanins in response to sucrose. Gene expression analysis indicated that expression of late, but not early, biosynthesis genes in the flavonoid pathway was dramatically reduced in gl2-1D but elevated in gl2-3 mutants. Further analysis showed that expression of some MBW component genes involved in the regulation of late biosynthesis genes was reduced in gl2-1D but elevated in gl2-3 mutants, and chromatin immunoprecipitation results indicated that some MBW component genes are targets of GL2. We also showed that GL2 functions as a transcriptional repressor. Altogether, these results indicate that GL2 negatively regulates anthocyanin biosynthesis in Arabidopsis by directly repressing the expression of some MBW component genes.},
doi = {10.1111/tpj.12887},
journal = {The Plant Journal},
number = 2,
volume = 83,
place = {United States},
year = {Wed Jun 17 00:00:00 EDT 2015},
month = {Wed Jun 17 00:00:00 EDT 2015}
}
Free Publicly Available Full Text
Publisher's Version of Record
Other availability
Search WorldCat to find libraries that may hold this journal
Cited by: 68 works
Citation information provided by
Web of Science
Web of Science
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.
Works referenced in this record:
Comprehensive identification of Arabidopsis thaliana MYB transcription factors interacting with R/B-like BHLH proteins: Systematic analysis of MYB/BHLH-interactions
journal, August 2004
- Zimmermann, Ilona M.; Heim, Marc A.; Weisshaar, Bernd
- The Plant Journal, Vol. 40, Issue 1
Photoregulation of Anthocyanin Synthesis: VIII. Effect of Light Pretreatments
journal, June 1984
- Mancinelli, Alberto L.
- Plant Physiology, Vol. 75, Issue 2
A Common Position-Dependent Mechanism Controls Cell-Type Patterning and GLABRA2 Regulation in the Root and Hypocotyl Epidermis of Arabidopsis
journal, May 1998
- Hung, Chen-Yi; Lin, Yan; Zhang, Meng
- Plant Physiology, Vol. 117, Issue 1
Arabidopsis Transient Expression Analysis Reveals that Activation of GLABRA2 May Require Concurrent Binding of GLABRA1 and GLABRA3 to the Promoter of GLABRA2
journal, October 2008
- Wang, S.; Chen, J. -G.
- Plant and Cell Physiology, Vol. 49, Issue 12
Characterization of Flavonol Synthase and Leucoanthocyanidin Dioxygenase Genes in Arabidopsis (Further Evidence for Differential Regulation of "Early" and "Late" Genes)
journal, April 1997
- Pelletier, M. K.; Murrell, J. R.; Shirley, B. W.
- Plant Physiology, Vol. 113, Issue 4
TRIPTYCHON and CAPRICE mediate lateral inhibition during trichome and root hair patterning in Arabidopsis
journal, October 2002
- Schellmann, S.; Schnittger, A.; Kirik, V.
- The EMBO Journal, Vol. 21, Issue 19
Transcriptional regulation of the Arabidopsis thaliana chalcone synthase gene
journal, May 1988
- Feinbaum, R. L.; Ausubel, F. M.
- Molecular and Cellular Biology, Vol. 8, Issue 5
The GLABRA2 gene encodes a homeo domain protein required for normal trichome development in Arabidopsis.
journal, June 1994
- Rerie, W. G.; Feldmann, K. A.; Marks, M. D.
- Genes & Development, Vol. 8, Issue 12
Functional Properties of a Phenylalanine Ammonia-Lyase Promoter from Arabidopsis
journal, September 1990
- Ohl, Stephan; Hedrick, Susan A.; Chory, Joanne
- The Plant Cell, Vol. 2, Issue 9
A Genetic Regulatory Network in the Development of Trichomes and Root Hairs
journal, June 2008
- Ishida, Tetsuya; Kurata, Tetsuya; Okada, Kiyotaka
- Annual Review of Plant Biology, Vol. 59, Issue 1
Participation of the Arabidopsis bHLH Factor GL3 in Trichome Initiation Regulatory Events
journal, September 2007
- Morohashi, Kengo; Zhao, Mingzhe; Yang, Manli
- Plant Physiology, Vol. 145, Issue 3
Repression Domains of Class II ERF Transcriptional Repressors Share an Essential Motif for Active Repression
journal, August 2001
- Ohta, M.
- THE PLANT CELL ONLINE, Vol. 13, Issue 8
Transcriptional control of flavonoid biosynthesis: Fine-tuning of the MYB-bHLH-WD40 (MBW) complex
journal, January 2014
- Li, Shutian
- Plant Signaling & Behavior, Vol. 9, Issue 1
AUX/IAA Proteins Are Active Repressors, and Their Stability and Activity Are Modulated by Auxin
journal, December 2001
- Tiwari, S. B.
- THE PLANT CELL ONLINE, Vol. 13, Issue 12
An ankyrin repeat protein is involved in anthocyanin biosynthesis in Arabidopsis
journal, April 2011
- Yoo, Jihye; Shin, Dong Ho; Cho, Man-Ho
- Physiologia Plantarum, Vol. 142, Issue 4
CPC, a Single-Repeat R3 MYB, Is a Negative Regulator of Anthocyanin Biosynthesis in Arabidopsis
journal, July 2009
- Zhu, Hui-Fen; Fitzsimmons, Karen; Khandelwal, Abha
- Molecular Plant, Vol. 2, Issue 4
Pathways for Epidermal Cell Differentiation via the Homeobox Gene GLABRA2 : Update on the Roles of the Classic Regulator F
journal, October 2012
- Qing, Lin; Aoyama, Takashi
- Journal of Integrative Plant Biology, Vol. 54, Issue 10
Genetic dissection of trichome cell development in Arabidopsis
journal, February 1994
- Hülskamp, Martin; Miséra, Simon; Jürgens, Gerd
- Cell, Vol. 76, Issue 3
cis-Element- and Transcriptome-Based Screening of Root Hair-Specific Genes and Their Functional Characterization in Arabidopsis
journal, May 2009
- Won, Su-Kyung; Lee, Yong-Ju; Lee, Ha-Yeon
- Plant Physiology, Vol. 150, Issue 3
Structure and Mechanism of Anthocyanidin Synthase from Arabidopsis thaliana
journal, January 2002
- Wilmouth, Rupert C.; Turnbull, Jonathan J.; Welford, Richard W. D.
- Structure, Vol. 10, Issue 1
The bHLH genes GLABRA3 (GL3) andENHANCER OF GLABRA3 (EGL3) specify epidermal cell fate in the Arabidopsis root
journal, December 2003
- Bernhardt, C.
- Development, Vol. 130, Issue 26
An efficient chromatin immunoprecipitation (ChIP) protocol for studying histone modifications in Arabidopsis plants
journal, May 2008
- Saleh, Abdelaty; Alvarez-Venegas, Raúl; Avramova, Zoya
- Nature Protocols, Vol. 3, Issue 6
Regulation of epidermal cell fate in Arabidopsis roots: the importance of multiple feedback loops
journal, January 2014
- Schiefelbein, John; Huang, Ling; Zheng, Xiaohua
- Frontiers in Plant Science, Vol. 5
The Arabidopsis sugar-insensitive mutants sis4 and sis5 are defective in abscisic acid synthesis and response
journal, September 2000
- Laby, Ron J.; Kincaid, M. Sean; Kim, Donggiun
- The Plant Journal, Vol. 23, Issue 5
Trichome Development in Arabidopsis thaliana. II. Isolation and Complementation of the GLABROUS1 Gene.
journal, November 1989
- Herman, P. L.; Marks, M. D.
- The Plant Cell
Comprehensive analysis of single-repeat R3 MYB proteins in epidermal cell patterning and their transcriptional regulation in Arabidopsis
text, January 2008
- Wang, Shucai; Hubbard, Leah; Chang, Ying
- BioMed Central
One, two, three…models for trichome patterning in Arabidopsis?
journal, October 2009
- Pesch, Marina; Hülskamp, Martin
- Current Opinion in Plant Biology, Vol. 12, Issue 5
A contradictory GLABRA3 allele helps define gene interactions controlling trichome development in Arabidopsis
journal, October 2003
- Esch, J. J.
- Development, Vol. 130, Issue 24
The TTG1-bHLH-MYB complex controls trichome cell fate and patterning through direct targeting of regulatory loci
journal, April 2008
- Zhao, M.; Morohashi, K.; Hatlestad, G.
- Development, Vol. 135, Issue 11
The Arabidopsis TT2 Gene Encodes an R2R3 MYB Domain Protein That Acts as a Key Determinant for Proanthocyanidin Accumulation in Developing Seed
journal, September 2001
- Nesi, Nathalie; Jond, Clarisse; Debeaujon, Isabelle
- The Plant Cell, Vol. 13, Issue 9
Modulation of Phospholipid Signaling by GLABRA2 in Root-Hair Pattern Formation
journal, May 2003
- Ohashi, Y.
- Science, Vol. 300, Issue 5624
Efficient isolation and mapping of Arabidopsis thaliana T-DNA insert junctions by thermal asymmetric interlaced PCR
journal, September 1995
- Liu, Yao-Guang; Mitsukawa, Norihiro; Oosumi, Teruko
- The Plant Journal, Vol. 8, Issue 3
Brassinosteroid Enhances Jasmonate-Induced Anthocyanin Accumulation in Arabidopsis Seedlings
journal, June 2011
- Peng, Zhihong; Han, Chengyun; Yuan, Libing
- Journal of Integrative Plant Biology, Vol. 53, Issue 8
Activation Tagging in Arabidopsis
journal, April 2000
- Weigel, Detlef; Ahn, Ji Hoon; Blázquez, Miguel A.
- Plant Physiology, Vol. 122, Issue 4
Cinnamate-4-Hydroxylase Expression in Arabidopsis (Regulation in Response to Development and the Environment)
journal, March 1997
- Bell-Lelong, D. A.; Cusumano, J. C.; Meyer, K.
- Plant Physiology, Vol. 113, Issue 3
Trichome Development in Arabidopsis thaliana. II. Isolation and Complementation of the GLABROUS1 Gene
journal, November 1989
- Herman, Patricia L.; Marks, M. David
- The Plant Cell, Vol. 1, Issue 11
Regulation of cell fate determination by single-repeat R3 MYB transcription factors in Arabidopsis
journal, April 2014
- Wang, Shucai; Chen, Jin-Gui
- Frontiers in Plant Science, Vol. 5
Effects of Ionizing Radiation on a Plant Genome: Analysis of Two Arabidopsis transparent testa Mutations
journal, March 1992
- Shirley, Brenda W.; Hanley, Susan; Goodman, Howard M.
- The Plant Cell, Vol. 4, Issue 3
WEREWOLF, a MYB-Related Protein in Arabidopsis, Is a Position-Dependent Regulator of Epidermal Cell Patterning
journal, November 1999
- Lee, Myeong Min; Schiefelbein, John
- Cell, Vol. 99, Issue 5
Regulation of the anthocyanin biosynthetic pathway by the TTG1/bHLH/Myb transcriptional complex in Arabidopsis seedlings
journal, March 2008
- Gonzalez, Antonio; Zhao, Mingzhe; Leavitt, John M.
- The Plant Journal, Vol. 53, Issue 5
Regulation of secondary cell wall biosynthesis by poplar R2R3 MYB transcription factor PtrMYB152 in Arabidopsis
journal, May 2014
- Wang, Shucai; Li, Eryang; Porth, Ilga
- Scientific Reports, Vol. 4, Issue 1
Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana
journal, December 1998
- Clough, Steven J.; Bent, Andrew F.
- The Plant Journal, Vol. 16, Issue 6, p. 735-743
Activation Tagging Identifies a Conserved MYB Regulator of Phenylpropanoid Biosynthesis
journal, December 2000
- Borevitz, Justin O.; Xia, Yiji; Blount, Jack
- The Plant Cell, Vol. 12, Issue 12
The TT8 Gene Encodes a Basic Helix-Loop-Helix Domain Protein Required for Expression of DFR and BAN Genes in Arabidopsis Siliques
journal, October 2000
- Nesi, Nathalie; Debeaujon, Isabelle; Jond, Clarisse
- The Plant Cell, Vol. 12, Issue 10
Control of trichome formation in Arabidopsis by poplar single-repeat R3 MYB transcription factors
journal, June 2014
- Zhou, Limei; Zheng, Kaijie; Wang, Xiaoyu
- Frontiers in Plant Science, Vol. 5
TT8 controls its own expression in a feedback regulation involving TTG1 and homologous MYB and bHLH factors, allowing a strong and cell-specific accumulation of flavonoids in Arabidopsis thaliana
journal, June 2006
- Baudry, Antoine; Caboche, Michel; Lepiniec, Loïc
- The Plant Journal, Vol. 46, Issue 5
The TRANSPARENT TESTA GLABRA1 Locus, Which Regulates Trichome Differentiation and Anthocyanin Biosynthesis in Arabidopsis, Encodes a WD40 Repeat Protein
journal, July 1999
- Walker, Amanda R.; Davison, Paul A.; Bolognesi-Winfield, Agnese C.
- The Plant Cell, Vol. 11, Issue 7
Differential regulation of closely related R2R3-MYB transcription factors controls flavonol accumulation in different parts of the Arabidopsis thaliana seedling: TFs of A. thaliana R2R3-MYB subgroup 7
journal, April 2007
- Stracke, Ralf; Ishihara, Hirofumi; Huep, Gunnar
- The Plant Journal, Vol. 50, Issue 4
The GLABRA2 homeodomain protein directly regulates CESA5 and XTH17 gene expression in Arabidopsis roots
journal, November 2009
- Tominaga-Wada, Rumi; Iwata, Mineko; Sugiyama, Junji
- The Plant Journal, Vol. 60, Issue 3
The TT8 Gene Encodes a Basic Helix-Loop-Helix Domain Protein Required for Expression of DFR and BAN Genes in Arabidopsis Siliques
journal, October 2000
- Nesi, Nathalie; Debeaujon, Isabelle; Jond, Clarisse
- The Plant Cell, Vol. 12, Issue 10
REF4 and RFR1, Subunits of the Transcriptional Coregulatory Complex Mediator, Are Required for Phenylpropanoid Homeostasis in Arabidopsis
journal, December 2011
- Bonawitz, Nicholas D.; Soltau, Whitney L.; Blatchley, Michael R.
- Journal of Biological Chemistry, Vol. 287, Issue 8
TTG1 complex MYBs, MYB5 and TT2, control outer seed coat differentiation
journal, January 2009
- Gonzalez, Antonio; Mendenhall, John; Huo, Yujia
- Developmental Biology, Vol. 325, Issue 2
Recent advances on the regulation of anthocyanin synthesis in reproductive organs
journal, September 2011
- Petroni, Katia; Tonelli, Chiara
- Plant Science, Vol. 181, Issue 3
Distinct relationships between GLABRA2 and single-repeat R3 MYB transcription factors in the regulation of trichome and root hair patterning in Arabidopsis
journal, October 2009
- Wang, Shucai; Barron, Christa; Schiefelbein, John
- New Phytologist, Vol. 185, Issue 2
The TRANSPARENT TESTA GLABRA1 Locus, Which Regulates Trichome Differentiation and Anthocyanin Biosynthesis in Arabidopsis, Encodes a WD40 Repeat Protein
journal, July 1999
- Walker, Amanda R.; Davison, Paul A.; Bolognesi-Winfield, Agnese C.
- The Plant Cell, Vol. 11, Issue 7
Functional properties of a phenylalanine ammonia-lyase promoter from Arabidopsis.
journal, September 1990
- Ohl, S.; Hedrick, S. A.; Chory, J.
- The Plant Cell, Vol. 2, Issue 9
The Arabidopsis TDS4 gene encodes leucoanthocyanidin dioxygenase (LDOX) and is essential for proanthocyanidin synthesis and vacuole development
journal, September 2003
- Abrahams, Sharon; Lee, Elizabeth; Walker, Amanda R.
- The Plant Journal, Vol. 35, Issue 5
Transcriptional regulation of secondary metabolite biosynthesis in plants
journal, November 2013
- Patra, Barunava; Schluttenhofer, Craig; Wu, Yongmei
- Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms, Vol. 1829, Issue 11
A Gene Regulatory Network for Root Epidermis Cell Differentiation in Arabidopsis
journal, January 2012
- Bruex, Angela; Kainkaryam, Raghunandan M.; Wieckowski, Yana
- PLoS Genetics, Vol. 8, Issue 1
The Jasmonate-ZIM-Domain Proteins Interact with the WD-Repeat/bHLH/MYB Complexes to Regulate Jasmonate-Mediated Anthocyanin Accumulation and Trichome Initiation in Arabidopsis thaliana
journal, May 2011
- Qi, Tiancong; Song, Susheng; Ren, Qingcuo
- The Plant Cell, Vol. 23, Issue 5
The Arabidopsis thaliana 4-coumarate:CoA ligase (4CL) gene: stress and developmentally regulated expression and nucleotide sequence of its cDNA
journal, August 1995
- Lee, Diana; Ellard, Mary; Wanner, Leslie A.
- Plant Molecular Biology, Vol. 28, Issue 5
The Arabidopsis MYB5 Transcription Factor Regulates Mucilage Synthesis, Seed Coat Development, and Trichome Morphogenesis
journal, January 2009
- Li, Song Feng; Milliken, Olga Nicolaou; Pham, Hanh
- The Plant Cell, Vol. 21, Issue 1
The Arabidopsis Athb-10 (GLABRA2) is an HD-Zip protein required for regulation of root hair development
journal, September 1996
- Cristina, Manlio; Sessa, Giovanna; Dolan, Liam
- The Plant Journal, Vol. 10, Issue 3
Complexity and robustness of the flavonoid transcriptional regulatory network revealed by comprehensive analyses of MYB-bHLH-WDR complexes and their targets in Arabidopsis seed
journal, December 2013
- Xu, Wenjia; Grain, Damaris; Bobet, Sophie
- New Phytologist, Vol. 202, Issue 1
Modulation of Cell Proliferation by Heterotrimeric G Protein in Arabidopsis
journal, June 2001
- Ullah, H.
- Science, Vol. 292, Issue 5524
A network of redundant bHLH proteins functions in all TTG1-dependent pathways of Arabidopsis
journal, October 2003
- Zhang, F.
- Development, Vol. 130, Issue 20
Arabidopsis Ovate Family Protein 1 is a transcriptional repressor that suppresses cell elongation: AtOFP1 suppresses cell elongation
journal, April 2007
- Wang, Shucai; Chang, Ying; Guo, Jianjun
- The Plant Journal, Vol. 50, Issue 5
Comprehensive analysis of single-repeat R3 MYB proteins in epidermal cell patterning and their transcriptional regulation in Arabidopsis
journal, January 2008
- Wang, Shucai; Hubbard, Leah; Chang, Ying
- BMC Plant Biology, Vol. 8, Issue 1
Role of Anthocyanidin Reductase, Encoded by BANYULS in Plant Flavonoid Biosynthesis
journal, January 2003
- Xie, D. -Y.
- Science, Vol. 299, Issue 5605
Effects of ionizing radiation on a plant genome: analysis of two Arabidopsis transparent testa mutations.
journal, March 1992
- Shirley, B. W.; Hanley, S.; Goodman, H. M.
- The Plant Cell, Vol. 4, Issue 3
The Arabidopsis Transcription Factor MYB12 Is a Flavonol-Specific Regulator of Phenylpropanoid Biosynthesis
journal, May 2005
- Mehrtens, Frank; Kranz, Harald; Bednarek, Pawel
- Plant Physiology, Vol. 138, Issue 2
Ethylene Suppression of Sugar-Induced Anthocyanin Pigmentation in Arabidopsis
journal, September 2010
- Jeong, Seok-Won; Das, Prasanta Kumar; Jeoung, Sae Chae
- Plant Physiology, Vol. 154, Issue 3
Sugar-Dependent Expression of the CHS-A Gene for Chalcone Synthase from Petunia in Transgenic Arabidopsis
journal, December 1991
- Tsukaya, Hirokazu; Ohshima, Toshiyuki; Naito, Satoshi
- Plant Physiology, Vol. 97, Issue 4
Transcriptional regulation of the Arabidopsis thaliana chalcone synthase gene.
journal, May 1988
- Feinbaum, R. L.; Ausubel, F. M.
- Molecular and Cellular Biology, Vol. 8, Issue 5
Analysis of Flavanone 3-Hydroxylase in Arabidopsis Seedlings (Coordinate Regulation with Chalcone Synthase and Chalcone Isomerase)
journal, May 1996
- Pelletier, M. K.; Shirley, B. W.
- Plant Physiology, Vol. 111, Issue 1
Arabidopsis glabra2 mutant seeds deficient in mucilage biosynthesis produce more oil: Mucilage-deficient gl2 seeds produce more oil
journal, October 2011
- Shi, Lin; Katavic, Vesna; Yu, Yuanyuan
- The Plant Journal, Vol. 69, Issue 1
Cell-fate specification in the epidermis: a common patterning mechanism in the root and shoot
journal, February 2003
- Schiefelbein, John
- Current Opinion in Plant Biology, Vol. 6, Issue 1
Isolation and Characterization of Mutants Defective in Seed Coat Mucilage Secretory Cell Development in Arabidopsis
journal, November 2001
- Western, Tamara L.; Burn, Joanne; Tan, Wei Ling
- Plant Physiology, Vol. 127, Issue 3
Creating a two-dimensional pattern de novo during Arabidopsis trichome and root hair initiation
journal, August 2004
- Pesch, Martina; Hülskamp, Martin
- Current Opinion in Genetics & Development, Vol. 14, Issue 4
The Homeobox Gene GLABRA2 Affects Seed Oil Content in Arabidopsis
journal, February 2006
- Shen, Bo; Sinkevicius, Kerstin W.; Selinger, David A.
- Plant Molecular Biology, Vol. 60, Issue 3
Functional characterization of TRICHOMELESS2, a new single-repeat R3 MYB transcription factor in the regulation of trichome patterning in Arabidopsis
journal, January 2011
- Gan, Lijun; Xia, Kai; Chen, Jin-Gui
- BMC Plant Biology, Vol. 11, Issue 1
HD-Zip Proteins GL2 and HDG11 Have Redundant Functions in Arabidopsis Trichomes, and GL2 Activates a Positive Feedback Loop via MYB23
journal, May 2014
- Khosla, Aashima; Paper, Janet M.; Boehler, Allison P.
- The Plant Cell, Vol. 26, Issue 5
Activation Tagging Identifies a Conserved MYB Regulator of Phenylpropanoid Biosynthesis
journal, December 2000
- Borevitz, Justin O.; Xia, Yiji; Blount, Jack
- The Plant Cell, Vol. 12, Issue 12
Nitrogen depletion and small R3-MYB transcription factors affecting anthocyanin accumulation in Arabidopsis leaves
journal, February 2014
- Nemie-Feyissa, Dugassa; Olafsdottir, Solveig Margret; Heidari, Behzad
- Phytochemistry, Vol. 98
Sucrose-Specific Induction of Anthocyanin Biosynthesis in Arabidopsis Requires the MYB75/PAP1 Gene
journal, November 2005
- Teng, Sheng; Keurentjes, Joost; Bentsink, Leónie
- Plant Physiology, Vol. 139, Issue 4
Aux/IAA Proteins Contain a Potent Transcriptional Repression Domain
journal, January 2004
- Tiwari, Shiv B.; Hagen, Gretchen; Guilfoyle, Tom J.
- The Plant Cell, Vol. 16, Issue 2
AUXIN RESPONSE FACTOR7 Restores the Expression of Auxin-Responsive Genes in Mutant Arabidopsis Leaf Mesophyll Protoplasts
journal, May 2005
- Wang, Shucai; Tiwari, Shiv B.; Hagen, Gretchen
- The Plant Cell, Vol. 17, Issue 7
Negative Regulation of Anthocyanin Biosynthesis in Arabidopsis by a miR156-Targeted SPL Transcription Factor
journal, April 2011
- Gou, Jin-Ying; Felippes, Felipe F.; Liu, Chang-Jun
- The Plant Cell, Vol. 23, Issue 4
The R2R3-MYB transcription factor MtMYB134 orchestrates flavonol biosynthesis in Medicago truncatula
journal, March 2021
- Naik, Jogindra; Rajput, Ruchika; Pucker, Boas
- Plant Molecular Biology, Vol. 106, Issue 1-2
The Arabidopsis TT2 Gene Encodes and R2R3 MYB Domain Protein That Acts as a Key Determinant for Proanthocyanidin Accumulation in Developing Seed
journal, September 2001
- Nesi, Nathalie; Jond, Clarisse; Debeaujon, Isabelle
- The Plant Cell, Vol. 13, Issue 9
GL3 Encodes a bHLH Protein That Regulates Trichome Development in Arabidopsis Through Interaction With GL1 and TTG1
journal, November 2000
- Payne, C. Thomas; Zhang, Fan; Lloyd, Alan M.
- Genetics, Vol. 156, Issue 3
Works referencing / citing this record:
A repressor motif-containing poplar R3 MYB-like transcription factor regulates epidermal cell fate determination and anthocyanin biosynthesis in Arabidopsis
journal, October 2016
- Hu, Qingnan; Yang, Li; Liu, Shanda
- Journal of Plant Biology, Vol. 59, Issue 5
Dissecting molecular and physiological response mechanisms to high solar radiation in cyanic and acyanic leaves: a case study on red and green basil
journal, April 2017
- Tattini, Massimiliano; Sebastiani, Federico; Brunetti, Cecilia
- Journal of Experimental Botany, Vol. 68, Issue 9
Identification of Candidate HY5-Dependent and -Independent Regulators of Anthocyanin Biosynthesis in Tomato
journal, December 2018
- Qiu, Zhengkun; Wang, Haijing; Li, Dongjing
- Plant and Cell Physiology, Vol. 60, Issue 3
COP9 signalosome subunit 5A affects phenylpropanoid metabolism, trichome formation and transcription of key genes of a regulatory tri-protein complex in Arabidopsis
journal, June 2018
- Wei, Shu; Li, Xiang; Gruber, Margaret Y.
- BMC Plant Biology, Vol. 18, Issue 1
RNA sequencing provides insights into the evolution of lettuce and the regulation of flavonoid biosynthesis
journal, December 2017
- Zhang, Lei; Su, Wenqing; Tao, Rong
- Nature Communications, Vol. 8, Issue 1
Ectopic expression of R3 MYB transcription factor gene OsTCL1 in Arabidopsis, but not rice, affects trichome and root hair formation
journal, January 2016
- Zheng, Kaijie; Tian, Hainan; Hu, Qingnan
- Scientific Reports, Vol. 6, Issue 1
An ABA down-regulated bHLH transcription repressor gene, bHLH129 regulates root elongation and ABA response when overexpressed in Arabidopsis
journal, December 2015
- Tian, Hainan; Guo, Hongyan; Dai, Xuemei
- Scientific Reports, Vol. 5, Issue 1
Constitutive Expression of OsIAA9 Affects Starch Granules Accumulation and Root Gravitropic Response in Arabidopsis
journal, December 2015
- Luo, Sha; Li, Qianqian; Liu, Shanda
- Frontiers in Plant Science, Vol. 6
Regulation of anthocyanin accumulation via MYB75/HAT1/TPL-mediated transcriptional repression
journal, March 2019
- Zheng, Ting; Tan, Wenrong; Yang, Huan
- PLOS Genetics, Vol. 15, Issue 3
Class III OFPs function in the ER signaling pathway to regulate plant growth and development in Arabidopsis
journal, December 2018
- Wang, Xutong; Wang, Wei; Wang, Jie
- Journal of Plant Interactions, Vol. 14, Issue 1
Critical analysis of protein signaling networks involved in the regulation of plant secondary metabolism: focus on anthocyanins
journal, February 2016
- Bulgakov, Victor P.; Avramenko, Tatiana V.; Tsitsiashvili, Gurami Sh.
- Critical Reviews in Biotechnology, Vol. 37, Issue 6
Genetic evidence suggests that GIS functions downstream of TCL1 to regulate trichome formation in Arabidopsis
journal, April 2018
- Zhang, Na; Yang, Li; Luo, Sha
- BMC Plant Biology, Vol. 18, Issue 1
T-6b allocates more assimilation product for oil synthesis and less for polysaccharide synthesis during the seed development of Arabidopsis thaliana
journal, January 2017
- Jin, Yunkai; Hu, Jia; Liu, Xun
- Biotechnology for Biofuels, Vol. 10, Issue 1
Efficient isolation of protoplasts from freesia callus and its application in transient expression assays
journal, July 2019
- Shan, Xiaotong; Li, Yueqing; Zhou, Liudi
- Plant Cell, Tissue and Organ Culture (PCTOC), Vol. 138, Issue 3
Genetic evidence suggests that GIS functions downstream of TCL1 to regulate trichome formation in Arabidopsis
journal, April 2018
- Zhang, Na; Yang, Li; Luo, Sha
- BMC Plant Biology, Vol. 18, Issue 1
Overexpression of ERF96, a small ethylene response factor gene, enhances salt tolerance in Arabidopsis
journal, December 2017
- Wang, X.; Hou, C.; Zheng, K.
- Biologia plantarum, Vol. 61, Issue 4
An ABA down-regulated bHLH transcription repressor gene, bHLH129 regulates root elongation and ABA response when overexpressed in Arabidopsis
journal, December 2015
- Tian, Hainan; Guo, Hongyan; Dai, Xuemei
- Scientific Reports, Vol. 5, Issue 1
The Small Ethylene Response Factor ERF96 is Involved in the Regulation of the Abscisic Acid Response in Arabidopsis
journal, November 2015
- Wang, Xiaoping; Liu, Shanda; Tian, Hainan
- Frontiers in Plant Science, Vol. 6
Two IIIf Clade-bHLHs from Freesia hybrida Play Divergent Roles in Flavonoid Biosynthesis and Trichome Formation when Ectopically Expressed in Arabidopsis
journal, July 2016
- Li, Yueqing; Shan, Xiaotong; Gao, Ruifang
- Scientific Reports, Vol. 6, Issue 1
Arabidopsis ACYL-COA-BINDING PROTEIN1 interacts with STEROL C4-METHYL OXIDASE1-2 to modulate gene expression of homeodomain-leucine zipper IV transcription factors
journal, December 2017
- Lung, Shiu-Cheung; Liao, Pan; Yeung, Edward C.
- New Phytologist, Vol. 218, Issue 1
A novel family of transcription factors conserved in angiosperms is required for ABA signalling
journal, September 2017
- Tian, Hainan; Chen, Siyu; Yang, Wenting
- Plant, Cell & Environment, Vol. 40, Issue 12
Critical analysis of protein signaling networks involved in the regulation of plant secondary metabolism: focus on anthocyanins
text, January 2016
- Bulgakov, Victor P.; Avramenko, Tatiana V.; Tsitsiashvili, Gurami Sh.
- Taylor & Francis
Two IIIf Clade-bHLHs from Freesia hybrida Play Divergent Roles in Flavonoid Biosynthesis and Trichome Formation when Ectopically Expressed in Arabidopsis
journal, July 2016
- Li, Yueqing; Shan, Xiaotong; Gao, Ruifang
- Scientific Reports, Vol. 6, Issue 1
Involvement of PACLOBUTRAZOL RESISTANCE6/KIDARI, an Atypical bHLH Transcription Factor, in Auxin Responses in Arabidopsis
journal, October 2017
- Zheng, Kaijie; Wang, Yating; Zhang, Na
- Frontiers in Plant Science, Vol. 8
Regulation of anthocyanin accumulation via MYB75/HAT1/TPL-mediated transcriptional repression
journal, March 2019
- Zheng, Ting; Tan, Wenrong; Yang, Huan
- PLOS Genetics, Vol. 15, Issue 3
Involvement of PACLOBUTRAZOL RESISTANCE6/KIDARI, an Atypical bHLH Transcription Factor, in Auxin Responses in Arabidopsis
journal, October 2017
- Zheng, Kaijie; Wang, Yating; Zhang, Na
- Frontiers in Plant Science, Vol. 8
Anthocyanins: From plant pigments to health benefits at mitochondrial level
journal, November 2019
- Bendokas, Vidmantas; Skemiene, Kristina; Trumbeckaite, Sonata
- Critical Reviews in Food Science and Nutrition, Vol. 60, Issue 19
The R 2 R 3 MYB transcription factor P av MYB 10.1 involves in anthocyanin biosynthesis and determines fruit skin colour in sweet cherry ( P runus avium L .)
journal, May 2016
- Jin, Wanmei; Wang, Hua; Li, Maofu
- Plant Biotechnology Journal, Vol. 14, Issue 11
COP9 signalosome subunit 5A affects phenylpropanoid metabolism, trichome formation and transcription of key genes of a regulatory tri-protein complex in Arabidopsis
journal, June 2018
- Wei, Shu; Li, Xiang; Gruber, Margaret Y.
- BMC Plant Biology, Vol. 18, Issue 1