Elevated temperature alters proteomic responses of individual organisms within a biofilm community
Abstract
Microbial communities that underpin global biogeochemical cycles will likely be influenced by elevated temperature associated with environmental change. Here, we test an approach to measure how elevated temperature impacts the physiology of individual microbial groups in a community context, using a model microbial-based ecosystem. The study is the first application of tandem mass tag (TMT)-based proteomics to a microbial community. We accurately, precisely and reproducibly quantified thousands of proteins in biofilms growing at 40, 43 and 46 °C. Elevated temperature led to upregulation of proteins involved in amino-acid metabolism at the level of individual organisms and the entire community. Proteins from related organisms differed in their relative abundance and functional responses to temperature. Elevated temperature repressed carbon fixation proteins from two Leptospirillum genotypes, whereas carbon fixation proteins were significantly upregulated at higher temperature by a third member of this genus. Leptospirillum group III bacteria may have been subject to viral stress at elevated temperature, which could lead to greater carbon turnover in the microbial food web through the release of viral lysate. Overall, these findings highlight the utility of proteomics-enabled community-based physiology studies, and provide a methodological framework for possible extension to additional mixed culture and environmental sample analyses.
- Authors:
-
[1];
- Univ. of California, Berkeley, CA (United States). Dept. of Earth and Planetary Science
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee-Oak Ridge National Lab., Knoxville, TN (United States). Graduate School of Genome Science and Technology
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Univ. of California, Berkeley, CA (United States). Dept. of Earth and Planetary Science; Univ. of California, Berkeley, CA (United States). Dept. of Environmental Science, Policy, and Management
- Publication Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States). Oak Ridge Leadership Computing Facility (OLCF); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC)
- OSTI Identifier:
- 1286738
- Alternate Identifier(s):
- OSTI ID: 1579330
- Grant/Contract Number:
- AC05-00OR22725; AC02-05CH11231
- Resource Type:
- Accepted Manuscript
- Journal Name:
- The ISME Journal
- Additional Journal Information:
- Journal Volume: 9; Journal Issue: 1; Journal ID: ISSN 1751-7362
- Publisher:
- Nature Publishing Group
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Mosier, Annika C., Li, Zhou, Thomas, Brian C., Hettich, Robert L., Pan, Chongle, and Banfield, Jillian F. Elevated temperature alters proteomic responses of individual organisms within a biofilm community. United States: N. p., 2014.
Web. doi:10.1038/ismej.2014.113.
Mosier, Annika C., Li, Zhou, Thomas, Brian C., Hettich, Robert L., Pan, Chongle, & Banfield, Jillian F. Elevated temperature alters proteomic responses of individual organisms within a biofilm community. United States. https://doi.org/10.1038/ismej.2014.113
Mosier, Annika C., Li, Zhou, Thomas, Brian C., Hettich, Robert L., Pan, Chongle, and Banfield, Jillian F. Tue .
"Elevated temperature alters proteomic responses of individual organisms within a biofilm community". United States. https://doi.org/10.1038/ismej.2014.113. https://www.osti.gov/servlets/purl/1286738.
@article{osti_1286738,
title = {Elevated temperature alters proteomic responses of individual organisms within a biofilm community},
author = {Mosier, Annika C. and Li, Zhou and Thomas, Brian C. and Hettich, Robert L. and Pan, Chongle and Banfield, Jillian F.},
abstractNote = {Microbial communities that underpin global biogeochemical cycles will likely be influenced by elevated temperature associated with environmental change. Here, we test an approach to measure how elevated temperature impacts the physiology of individual microbial groups in a community context, using a model microbial-based ecosystem. The study is the first application of tandem mass tag (TMT)-based proteomics to a microbial community. We accurately, precisely and reproducibly quantified thousands of proteins in biofilms growing at 40, 43 and 46 °C. Elevated temperature led to upregulation of proteins involved in amino-acid metabolism at the level of individual organisms and the entire community. Proteins from related organisms differed in their relative abundance and functional responses to temperature. Elevated temperature repressed carbon fixation proteins from two Leptospirillum genotypes, whereas carbon fixation proteins were significantly upregulated at higher temperature by a third member of this genus. Leptospirillum group III bacteria may have been subject to viral stress at elevated temperature, which could lead to greater carbon turnover in the microbial food web through the release of viral lysate. Overall, these findings highlight the utility of proteomics-enabled community-based physiology studies, and provide a methodological framework for possible extension to additional mixed culture and environmental sample analyses.},
doi = {10.1038/ismej.2014.113},
journal = {The ISME Journal},
number = 1,
volume = 9,
place = {United States},
year = {Tue Jul 22 00:00:00 EDT 2014},
month = {Tue Jul 22 00:00:00 EDT 2014}
}
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