Structure of faustovirus, a large dsDNA virus
Abstract
Many viruses protect their genome with a combination of a protein shell with or without a membrane layer. In this paper, we describe the structure of faustovirus, the first DNA virus (to our knowledge) that has been found to use two protein shells to encapsidate and protect its genome. The crystal structure of the major capsid protein, in combination with cryo-electron microscopy structures of two different maturation stages of the virus, shows that the outer virus shell is composed of a double jelly-roll protein that can be found in many double-stranded DNA viruses. The structure of the repeating hexameric unit of the inner shell is different from all other known capsid proteins. In addition to the unique architecture, the region of the genome that encodes the major capsid protein stretches over 17,000 bp and contains a large number of introns and exons. Finally, this complexity might help the virus to rapidly adapt to new environments or hosts.
- Authors:
-
- Purdue Univ., West Lafayette, IN (United States). Dept. of Biological Sciences
- Aix-Marseille Univ., Marseille (France). Research Inst. for Development. Dept. of Research on Emerging Infectious and Tropical Diseases (URMITE)
- Purdue Univ., West Lafayette, IN (United States). Dept. of Chemistry
- Publication Date:
- Research Org.:
- Purdue Univ., West Lafayette, IN (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES); National Inst. of Health (NIH) (United States)
- Contributing Org.:
- Aix-Marseille Univ., Marseille (France)
- OSTI Identifier:
- 1258668
- Grant/Contract Number:
- AC02-06CH11357; AI011219
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Proceedings of the National Academy of Sciences of the United States of America
- Additional Journal Information:
- Journal Volume: 113; Journal Issue: 22; Journal ID: ISSN 0027-8424
- Publisher:
- National Academy of Sciences, Washington, DC (United States)
- Country of Publication:
- United States
- Language:
- ENGLISH
- Subject:
- 60 APPLIED LIFE SCIENCES; 59 BASIC BIOLOGICAL SCIENCES; faustovirus; structure; double-protein shell; major capsid protein
Citation Formats
Klose, Thomas, Reteno, Dorine G., Benamar, Samia, Hollerbach, Adam, Colson, Philippe, La Scola, Bernard, and Rossmann, Michael G. Structure of faustovirus, a large dsDNA virus. United States: N. p., 2016.
Web. doi:10.1073/pnas.1523999113.
Klose, Thomas, Reteno, Dorine G., Benamar, Samia, Hollerbach, Adam, Colson, Philippe, La Scola, Bernard, & Rossmann, Michael G. Structure of faustovirus, a large dsDNA virus. United States. https://doi.org/10.1073/pnas.1523999113
Klose, Thomas, Reteno, Dorine G., Benamar, Samia, Hollerbach, Adam, Colson, Philippe, La Scola, Bernard, and Rossmann, Michael G. Mon .
"Structure of faustovirus, a large dsDNA virus". United States. https://doi.org/10.1073/pnas.1523999113. https://www.osti.gov/servlets/purl/1258668.
@article{osti_1258668,
title = {Structure of faustovirus, a large dsDNA virus},
author = {Klose, Thomas and Reteno, Dorine G. and Benamar, Samia and Hollerbach, Adam and Colson, Philippe and La Scola, Bernard and Rossmann, Michael G.},
abstractNote = {Many viruses protect their genome with a combination of a protein shell with or without a membrane layer. In this paper, we describe the structure of faustovirus, the first DNA virus (to our knowledge) that has been found to use two protein shells to encapsidate and protect its genome. The crystal structure of the major capsid protein, in combination with cryo-electron microscopy structures of two different maturation stages of the virus, shows that the outer virus shell is composed of a double jelly-roll protein that can be found in many double-stranded DNA viruses. The structure of the repeating hexameric unit of the inner shell is different from all other known capsid proteins. In addition to the unique architecture, the region of the genome that encodes the major capsid protein stretches over 17,000 bp and contains a large number of introns and exons. Finally, this complexity might help the virus to rapidly adapt to new environments or hosts.},
doi = {10.1073/pnas.1523999113},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
number = 22,
volume = 113,
place = {United States},
year = {Mon May 16 00:00:00 EDT 2016},
month = {Mon May 16 00:00:00 EDT 2016}
}
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