Insights into the respiratory tract microbiota of patients with cystic fibrosis during early Pseudomonas aeruginosa colonization
Abstract
Pseudomonas aeruginosa plays a major role in cystic fibrosis (CF) progression. Therefore, it is important to understand the initial steps of P. aeruginosa infection. The structure and dynamics of CF respiratory tract microbial communities during the early stages of P. aeruginosa colonization were characterized by pyrosequencing and cloning-sequencing. The respiratory microbiota showed high diversity, related to the young age of the CF cohort (mean age 10 years). Wide inter- and intra-individual variations were revealed. A common core microbiota of 5 phyla and 13 predominant genera was found, the majority of which were obligate anaerobes. A few genera were significantly more prevalent in patients never infected by P. aeruginosa. Persistence of an anaerobic core microbiota regardless of P. aeruginosa status suggests a major role of certain anaerobes in the pathophysiology of lung infections in CF. Some genera may be potential biomarkers of pulmonary infection state.
- Authors:
-
- Univ. of Brest, Brest Cedex (France)
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States); Research and Development Luminy Biotech Enterprises, Marseille (France)
- Univ. of Brest, Brest Cedex (France); Univ. Hospital of Brest, Brest (France)
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
- Univ. Hospital of Brest, Brest (France)
- CF Center, Roscoff (France)
- Publication Date:
- Research Org.:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
- Sponsoring Org.:
- USDOE; French Cystic Fibrosis Association: 'Vaincre la Mucoviscidose'; French Ministry of Higher Education and Research (Ministère de l’Enseignement Supérieur et de la Recherche: MESR, France)
- OSTI Identifier:
- 1212084
- Grant/Contract Number:
- AC05-76RL01830
- Resource Type:
- Accepted Manuscript
- Journal Name:
- SpringerPlus
- Additional Journal Information:
- Journal Volume: 4; Journal Issue: 1; Journal ID: ISSN 2193-1801
- Publisher:
- Springer
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; cystic fibrosis; respiratory tract microbiota; Pseudomonas aeruginosa; early colonization; anaerobes; respiratory viruses
Citation Formats
Keravec, Marlène, Mounier, Jérôme, Prestat, Emmanuel, Vallet, Sophie, Jansson, Janet K., Burgaud, Gaëtan, Rosec, Sylvain, Gouriou, Stéphanie, Rault, Gilles, Coton, Emmanuel, Barbier, Georges, and Héry-Arnaud, Geneviève. Insights into the respiratory tract microbiota of patients with cystic fibrosis during early Pseudomonas aeruginosa colonization. United States: N. p., 2015.
Web. doi:10.1186/s40064-015-1207-0.
Keravec, Marlène, Mounier, Jérôme, Prestat, Emmanuel, Vallet, Sophie, Jansson, Janet K., Burgaud, Gaëtan, Rosec, Sylvain, Gouriou, Stéphanie, Rault, Gilles, Coton, Emmanuel, Barbier, Georges, & Héry-Arnaud, Geneviève. Insights into the respiratory tract microbiota of patients with cystic fibrosis during early Pseudomonas aeruginosa colonization. United States. https://doi.org/10.1186/s40064-015-1207-0
Keravec, Marlène, Mounier, Jérôme, Prestat, Emmanuel, Vallet, Sophie, Jansson, Janet K., Burgaud, Gaëtan, Rosec, Sylvain, Gouriou, Stéphanie, Rault, Gilles, Coton, Emmanuel, Barbier, Georges, and Héry-Arnaud, Geneviève. Sun .
"Insights into the respiratory tract microbiota of patients with cystic fibrosis during early Pseudomonas aeruginosa colonization". United States. https://doi.org/10.1186/s40064-015-1207-0. https://www.osti.gov/servlets/purl/1212084.
@article{osti_1212084,
title = {Insights into the respiratory tract microbiota of patients with cystic fibrosis during early Pseudomonas aeruginosa colonization},
author = {Keravec, Marlène and Mounier, Jérôme and Prestat, Emmanuel and Vallet, Sophie and Jansson, Janet K. and Burgaud, Gaëtan and Rosec, Sylvain and Gouriou, Stéphanie and Rault, Gilles and Coton, Emmanuel and Barbier, Georges and Héry-Arnaud, Geneviève},
abstractNote = {Pseudomonas aeruginosa plays a major role in cystic fibrosis (CF) progression. Therefore, it is important to understand the initial steps of P. aeruginosa infection. The structure and dynamics of CF respiratory tract microbial communities during the early stages of P. aeruginosa colonization were characterized by pyrosequencing and cloning-sequencing. The respiratory microbiota showed high diversity, related to the young age of the CF cohort (mean age 10 years). Wide inter- and intra-individual variations were revealed. A common core microbiota of 5 phyla and 13 predominant genera was found, the majority of which were obligate anaerobes. A few genera were significantly more prevalent in patients never infected by P. aeruginosa. Persistence of an anaerobic core microbiota regardless of P. aeruginosa status suggests a major role of certain anaerobes in the pathophysiology of lung infections in CF. Some genera may be potential biomarkers of pulmonary infection state.},
doi = {10.1186/s40064-015-1207-0},
journal = {SpringerPlus},
number = 1,
volume = 4,
place = {United States},
year = {Sun Aug 09 00:00:00 EDT 2015},
month = {Sun Aug 09 00:00:00 EDT 2015}
}
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