Phycobilisome protein ApcG interacts with PSII and regulates energy transfer in Synechocystis
Abstract
Abstract Photosynthetic organisms harvest light using pigment–protein complexes. In cyanobacteria, these are water-soluble antennae known as phycobilisomes (PBSs). The light absorbed by PBS is transferred to the photosystems in the thylakoid membrane to drive photosynthesis. The energy transfer between these complexes implies that protein–protein interactions allow the association of PBS with the photosystems. However, the specific proteins involved in the interaction of PBS with the photosystems are not fully characterized. Here, we show in Synechocystis sp. PCC 6803 that the recently discovered PBS linker protein ApcG (sll1873) interacts specifically with PSII through its N-terminal region. Growth of cyanobacteria is impaired in apcG deletion strains under light-limiting conditions. Furthermore, complementation of these strains using a phospho-mimicking version of ApcG causes reduced growth under normal growth conditions. Interestingly, the interaction of ApcG with PSII is affected when a phospho-mimicking version of ApcG is used, targeting the positively charged residues interacting with the thylakoid membrane, suggesting a regulatory role mediated by phosphorylation of ApcG. Low-temperature fluorescence measurements showed decreased PSI fluorescence in apcG deletion and complementation strains. The PSI fluorescence was the lowest in the phospho-mimicking complementation strain, while the pull-down experiment showed no interaction of ApcG with PSI under any tested condition.more »
- Publication Date:
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 2222550
- Grant/Contract Number:
- 449B
- Resource Type:
- Publisher's Accepted Manuscript
- Journal Name:
- Plant Physiology (Bethesda)
- Additional Journal Information:
- Journal Name: Plant Physiology (Bethesda); Journal ID: ISSN 0032-0889
- Publisher:
- Oxford University Press
- Country of Publication:
- United States
- Language:
- English
Citation Formats
Espinoza-Corral, Roberto, Iwai, Masakazu, Zavřel, Tomáš, Lechno-Yossef, Sigal, Sutter, Markus, Červený, Jan, Niyogi, Krishna K., and Kerfeld, Cheryl A. Phycobilisome protein ApcG interacts with PSII and regulates energy transfer in Synechocystis. United States: N. p., 2023.
Web. doi:10.1093/plphys/kiad615.
Espinoza-Corral, Roberto, Iwai, Masakazu, Zavřel, Tomáš, Lechno-Yossef, Sigal, Sutter, Markus, Červený, Jan, Niyogi, Krishna K., & Kerfeld, Cheryl A. Phycobilisome protein ApcG interacts with PSII and regulates energy transfer in Synechocystis. United States. https://doi.org/10.1093/plphys/kiad615
Espinoza-Corral, Roberto, Iwai, Masakazu, Zavřel, Tomáš, Lechno-Yossef, Sigal, Sutter, Markus, Červený, Jan, Niyogi, Krishna K., and Kerfeld, Cheryl A. Thu .
"Phycobilisome protein ApcG interacts with PSII and regulates energy transfer in Synechocystis". United States. https://doi.org/10.1093/plphys/kiad615.
@article{osti_2222550,
title = {Phycobilisome protein ApcG interacts with PSII and regulates energy transfer in Synechocystis},
author = {Espinoza-Corral, Roberto and Iwai, Masakazu and Zavřel, Tomáš and Lechno-Yossef, Sigal and Sutter, Markus and Červený, Jan and Niyogi, Krishna K. and Kerfeld, Cheryl A.},
abstractNote = {Abstract Photosynthetic organisms harvest light using pigment–protein complexes. In cyanobacteria, these are water-soluble antennae known as phycobilisomes (PBSs). The light absorbed by PBS is transferred to the photosystems in the thylakoid membrane to drive photosynthesis. The energy transfer between these complexes implies that protein–protein interactions allow the association of PBS with the photosystems. However, the specific proteins involved in the interaction of PBS with the photosystems are not fully characterized. Here, we show in Synechocystis sp. PCC 6803 that the recently discovered PBS linker protein ApcG (sll1873) interacts specifically with PSII through its N-terminal region. Growth of cyanobacteria is impaired in apcG deletion strains under light-limiting conditions. Furthermore, complementation of these strains using a phospho-mimicking version of ApcG causes reduced growth under normal growth conditions. Interestingly, the interaction of ApcG with PSII is affected when a phospho-mimicking version of ApcG is used, targeting the positively charged residues interacting with the thylakoid membrane, suggesting a regulatory role mediated by phosphorylation of ApcG. Low-temperature fluorescence measurements showed decreased PSI fluorescence in apcG deletion and complementation strains. The PSI fluorescence was the lowest in the phospho-mimicking complementation strain, while the pull-down experiment showed no interaction of ApcG with PSI under any tested condition. Our results highlight the importance of ApcG for selectively directing energy harvested by the PBS and imply that the phosphorylation status of ApcG plays a role in regulating energy transfer from PSII to PSI.},
doi = {10.1093/plphys/kiad615},
journal = {Plant Physiology (Bethesda)},
number = ,
volume = ,
place = {United States},
year = {Thu Nov 16 00:00:00 EST 2023},
month = {Thu Nov 16 00:00:00 EST 2023}
}
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