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Title: Proteomic Insights into Phycobilisome Degradation, A Selective and Tightly Controlled Process in The Fast-Growing Cyanobacterium Synechococcus elongatus UTEX 2973

Abstract

Phycobilisomes (PBSs) are large (3–5 megadalton) pigment-protein complexes in cyanobacteria that associate with thylakoid membranes and harvest light primarily for photosystem II. PBSs consist of highly ordered assemblies of pigmented phycobiliproteins (PBPs) and linker proteins that can account for up to half of the soluble protein in cells. Cyanobacteria adjust to changing environmental conditions by modulating PBS size and number. In response to nutrient depletion such as nitrogen (N) deprivation, PBSs are degraded in an extensive, tightly controlled, and reversible process. In Synechococcus elongatus UTEX 2973, a fast-growing cyanobacterium with a doubling time of two hours, the process of PBS degradation is very rapid, with 80% of PBSs per cell degraded in six hours under optimal light and CO2 conditions. Proteomic analysis during PBS degradation and re-synthesis revealed multiple proteoforms of PBPs with partially degraded phycocyanobilin (PCB) pigments. NblA, a small proteolysis adaptor essential for PBS degradation, was characterized and validated with targeted mass spectrometry. NblA levels rose from essentially 0 to 25,000 copies per cell within 30 min of N depletion, and correlated with the rate of decrease in phycocyanin (PC). Implications of this correlation on the overall mechanism of PBS degradation during N deprivation are discussed.


Citation Formats

Nagarajan, Aparna, Zhou, Mowei, Nguyen, Amelia Y., Liberton, Michelle, Kedia, Komal, Shi, Tujin, Piehowski, Paul, Shukla, Anil, Fillmore, Thomas L., Nicora, Carrie, Smith, Richard D., Koppenaal, David W., Jacobs, Jon M., and Pakrasi, Himadri B. Proteomic Insights into Phycobilisome Degradation, A Selective and Tightly Controlled Process in The Fast-Growing Cyanobacterium Synechococcus elongatus UTEX 2973. Switzerland: N. p., 2019. Web. doi:10.3390/biom9080374.
Nagarajan, Aparna, Zhou, Mowei, Nguyen, Amelia Y., Liberton, Michelle, Kedia, Komal, Shi, Tujin, Piehowski, Paul, Shukla, Anil, Fillmore, Thomas L., Nicora, Carrie, Smith, Richard D., Koppenaal, David W., Jacobs, Jon M., & Pakrasi, Himadri B. Proteomic Insights into Phycobilisome Degradation, A Selective and Tightly Controlled Process in The Fast-Growing Cyanobacterium Synechococcus elongatus UTEX 2973. Switzerland. doi:10.3390/biom9080374.
Nagarajan, Aparna, Zhou, Mowei, Nguyen, Amelia Y., Liberton, Michelle, Kedia, Komal, Shi, Tujin, Piehowski, Paul, Shukla, Anil, Fillmore, Thomas L., Nicora, Carrie, Smith, Richard D., Koppenaal, David W., Jacobs, Jon M., and Pakrasi, Himadri B. Fri . "Proteomic Insights into Phycobilisome Degradation, A Selective and Tightly Controlled Process in The Fast-Growing Cyanobacterium Synechococcus elongatus UTEX 2973". Switzerland. doi:10.3390/biom9080374.
@article{osti_1557873,
title = {Proteomic Insights into Phycobilisome Degradation, A Selective and Tightly Controlled Process in The Fast-Growing Cyanobacterium Synechococcus elongatus UTEX 2973},
author = {Nagarajan, Aparna and Zhou, Mowei and Nguyen, Amelia Y. and Liberton, Michelle and Kedia, Komal and Shi, Tujin and Piehowski, Paul and Shukla, Anil and Fillmore, Thomas L. and Nicora, Carrie and Smith, Richard D. and Koppenaal, David W. and Jacobs, Jon M. and Pakrasi, Himadri B.},
abstractNote = {Phycobilisomes (PBSs) are large (3–5 megadalton) pigment-protein complexes in cyanobacteria that associate with thylakoid membranes and harvest light primarily for photosystem II. PBSs consist of highly ordered assemblies of pigmented phycobiliproteins (PBPs) and linker proteins that can account for up to half of the soluble protein in cells. Cyanobacteria adjust to changing environmental conditions by modulating PBS size and number. In response to nutrient depletion such as nitrogen (N) deprivation, PBSs are degraded in an extensive, tightly controlled, and reversible process. In Synechococcus elongatus UTEX 2973, a fast-growing cyanobacterium with a doubling time of two hours, the process of PBS degradation is very rapid, with 80% of PBSs per cell degraded in six hours under optimal light and CO2 conditions. Proteomic analysis during PBS degradation and re-synthesis revealed multiple proteoforms of PBPs with partially degraded phycocyanobilin (PCB) pigments. NblA, a small proteolysis adaptor essential for PBS degradation, was characterized and validated with targeted mass spectrometry. NblA levels rose from essentially 0 to 25,000 copies per cell within 30 min of N depletion, and correlated with the rate of decrease in phycocyanin (PC). Implications of this correlation on the overall mechanism of PBS degradation during N deprivation are discussed.},
doi = {10.3390/biom9080374},
journal = {Biomolecules},
number = 8,
volume = 9,
place = {Switzerland},
year = {2019},
month = {8}
}

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DOI: 10.3390/biom9080374

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