Engineering transcriptional regulation of pentose metabolism in Rhodosporidium toruloides for improved conversion of xylose to bioproducts
Abstract
Efficient conversion of pentose sugars remains a significant barrier to the replacement of petroleum-derived chemicals with plant biomass-derived bioproducts. While the oleaginous yeast Rhodosporidium toruloides (also known as Rhodotorula toruloides) has a relatively robust native metabolism of pentose sugars compared to other wild yeasts, faster assimilation of those sugars will be required for industrial utilization of pentoses. To increase the rate of pentose assimilation in R. toruloides, we leveraged previously reported high-throughput fitness data to identify potential regulators of pentose catabolism. Two genes were selected for further investigation, a putative transcription factor (RTO4_12978, Pnt1) and a homolog of a glucose transceptor involved in carbon catabolite repression (RTO4_11990). Overexpression of Pnt1 increased the specific growth rate approximately twofold early in cultures on xylose and increased the maximum specific growth by 18% while decreasing accumulation of arabitol and xylitol in fast-growing cultures. Improved growth dynamics on xylose translated to a 120% increase in the overall rate of xylose conversion to fatty alcohols in batch culture. Proteomic analysis confirmed that Pnt1 is a major regulator of pentose catabolism in R. toruloides. Deletion of RTO4_11990 increased the growth rate on xylose, but did not relieve carbon catabolite repression in the presence of glucose. Carbonmore »
- Authors:
- Publication Date:
- Research Org.:
- Pacific Northwest National Laboratory (PNNL), Richland, WA (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States); Sandia National Lab. (SNL-CA), Livermore, CA (United States); Environmental Molecular Sciences Laboratory (EMSL), Richland, WA (United States)
- Sponsoring Org.:
- USDOE National Nuclear Security Administration (NNSA); USDOE Office of Energy Efficiency and Renewable Energy (EERE), Office of Sustainable Transportation. Bioenergy Technologies Office (BETO); USDOE Office of Science (SC), Basic Energy Sciences (BES). Scientific User Facilities (SUF); USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1993646
- Alternate Identifier(s):
- OSTI ID: 2008018; OSTI ID: 2311349
- Report Number(s):
- PNNL-SA-177272; SAND-2023-09654J
Journal ID: ISSN 1475-2859; 144; PII: 2148
- Grant/Contract Number:
- AC05-76RL01830; NA0003525; AC02-05CH11231
- Resource Type:
- Published Article
- Journal Name:
- Microbial Cell Factories
- Additional Journal Information:
- Journal Name: Microbial Cell Factories Journal Volume: 22 Journal Issue: 1; Journal ID: ISSN 1475-2859
- Publisher:
- Springer Science + Business Media
- Country of Publication:
- United Kingdom
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Rhodosporidium toruloides; fatty alcohol; xylose metabolism; carbon catabolite repression; transcriptional regulation; proteomics
Citation Formats
Coradetti, Samuel T., Adamczyk, Paul A., Liu, Di, Gao, Yuqian, Otoupal, Peter B., Geiselman, Gina M., Webb-Robertson, Bobbie-Jo M., Burnet, Meagan C., Kim, Young-Mo, Burnum-Johnson, Kristin E., Magnuson, Jon, and Gladden, John M. Engineering transcriptional regulation of pentose metabolism in Rhodosporidium toruloides for improved conversion of xylose to bioproducts. United Kingdom: N. p., 2023.
Web. doi:10.1186/s12934-023-02148-5.
Coradetti, Samuel T., Adamczyk, Paul A., Liu, Di, Gao, Yuqian, Otoupal, Peter B., Geiselman, Gina M., Webb-Robertson, Bobbie-Jo M., Burnet, Meagan C., Kim, Young-Mo, Burnum-Johnson, Kristin E., Magnuson, Jon, & Gladden, John M. Engineering transcriptional regulation of pentose metabolism in Rhodosporidium toruloides for improved conversion of xylose to bioproducts. United Kingdom. https://doi.org/10.1186/s12934-023-02148-5
Coradetti, Samuel T., Adamczyk, Paul A., Liu, Di, Gao, Yuqian, Otoupal, Peter B., Geiselman, Gina M., Webb-Robertson, Bobbie-Jo M., Burnet, Meagan C., Kim, Young-Mo, Burnum-Johnson, Kristin E., Magnuson, Jon, and Gladden, John M. Thu .
"Engineering transcriptional regulation of pentose metabolism in Rhodosporidium toruloides for improved conversion of xylose to bioproducts". United Kingdom. https://doi.org/10.1186/s12934-023-02148-5.
@article{osti_1993646,
title = {Engineering transcriptional regulation of pentose metabolism in Rhodosporidium toruloides for improved conversion of xylose to bioproducts},
author = {Coradetti, Samuel T. and Adamczyk, Paul A. and Liu, Di and Gao, Yuqian and Otoupal, Peter B. and Geiselman, Gina M. and Webb-Robertson, Bobbie-Jo M. and Burnet, Meagan C. and Kim, Young-Mo and Burnum-Johnson, Kristin E. and Magnuson, Jon and Gladden, John M.},
abstractNote = {Efficient conversion of pentose sugars remains a significant barrier to the replacement of petroleum-derived chemicals with plant biomass-derived bioproducts. While the oleaginous yeast Rhodosporidium toruloides (also known as Rhodotorula toruloides) has a relatively robust native metabolism of pentose sugars compared to other wild yeasts, faster assimilation of those sugars will be required for industrial utilization of pentoses. To increase the rate of pentose assimilation in R. toruloides, we leveraged previously reported high-throughput fitness data to identify potential regulators of pentose catabolism. Two genes were selected for further investigation, a putative transcription factor (RTO4_12978, Pnt1) and a homolog of a glucose transceptor involved in carbon catabolite repression (RTO4_11990). Overexpression of Pnt1 increased the specific growth rate approximately twofold early in cultures on xylose and increased the maximum specific growth by 18% while decreasing accumulation of arabitol and xylitol in fast-growing cultures. Improved growth dynamics on xylose translated to a 120% increase in the overall rate of xylose conversion to fatty alcohols in batch culture. Proteomic analysis confirmed that Pnt1 is a major regulator of pentose catabolism in R. toruloides. Deletion of RTO4_11990 increased the growth rate on xylose, but did not relieve carbon catabolite repression in the presence of glucose. Carbon catabolite repression signaling networks remain poorly characterized in R. toruloides and likely comprise a different set of proteins than those mainly characterized in ascomycete fungi.},
doi = {10.1186/s12934-023-02148-5},
journal = {Microbial Cell Factories},
number = 1,
volume = 22,
place = {United Kingdom},
year = {Thu Aug 03 00:00:00 EDT 2023},
month = {Thu Aug 03 00:00:00 EDT 2023}
}
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