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Title: Production of galactitol from galactose by the oleaginous yeast Rhodosporidium toruloides IFO0880

Abstract

Sugar alcohols are commonly used as low-calorie sweeteners and can serve as potential building blocks for bio-based chemicals. Previous work has shown that the oleaginous yeast Rhodosporidium toruloides IFO0880 can natively produce arabitol from xylose at relatively high titers, suggesting that it may be a useful host for sugar alcohol production. In this work, we explored whether R. toruloides can produce additional sugar alcohols. Rhodosporidium toruloides is able to produce galactitol from galactose. During growth in nitrogen-rich medium, R. toruloides produced 3.2 ± 0.6 g/L, and 8.4 ± 0.8 g/L galactitol from 20 to 40 g/L galactose, respectively. In addition, R. toruloides was able to produce galactitol from galactose at reduced titers during growth in nitrogen-poor medium, which also induces lipid production. These results suggest that R. toruloides can potentially be used for the co-production of lipids and galactitol from galactose. We further characterized the mechanism for galactitol production, including identifying and biochemically characterizing the critical aldose reductase. Intracellular metabolite analysis was also performed to further understand galactose metabolism. Rhodosporidium toruloides has traditionally been used for the production of lipids and lipid-based chemicals. Our work demonstrates that R. toruloides can also produce galactitol, which can be used to produce polymersmore » with applications in medicine and as a precursor for anti-cancer drugs. Collectively, our results further establish that R. toruloidescan produce multiple value-added chemicals from a wide range of sugars.« less

Authors:
; ; ; ;
Publication Date:
Research Org.:
Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), Urbana, IL (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
OSTI Identifier:
1618767
Alternate Identifier(s):
OSTI ID: 1571254
Grant/Contract Number:  
SC0018420
Resource Type:
Published Article
Journal Name:
Biotechnology for Biofuels
Additional Journal Information:
Journal Name: Biotechnology for Biofuels Journal Volume: 12 Journal Issue: 1; Journal ID: ISSN 1754-6834
Publisher:
Springer Science + Business Media
Country of Publication:
Netherlands
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Rhodosporidium toruloides, galactose, galactitol, lipid, aldose reductase, metabolite profiling

Citation Formats

Jagtap, Sujit Sadashiv, Bedekar, Ashwini Ashok, Liu, Jing-Jing, Jin, Yong-Su, and Rao, Christopher V. Production of galactitol from galactose by the oleaginous yeast Rhodosporidium toruloides IFO0880. Netherlands: N. p., 2019. Web. doi:10.1186/s13068-019-1586-5.
Jagtap, Sujit Sadashiv, Bedekar, Ashwini Ashok, Liu, Jing-Jing, Jin, Yong-Su, & Rao, Christopher V. Production of galactitol from galactose by the oleaginous yeast Rhodosporidium toruloides IFO0880. Netherlands. doi:https://doi.org/10.1186/s13068-019-1586-5
Jagtap, Sujit Sadashiv, Bedekar, Ashwini Ashok, Liu, Jing-Jing, Jin, Yong-Su, and Rao, Christopher V. Fri . "Production of galactitol from galactose by the oleaginous yeast Rhodosporidium toruloides IFO0880". Netherlands. doi:https://doi.org/10.1186/s13068-019-1586-5.
@article{osti_1618767,
title = {Production of galactitol from galactose by the oleaginous yeast Rhodosporidium toruloides IFO0880},
author = {Jagtap, Sujit Sadashiv and Bedekar, Ashwini Ashok and Liu, Jing-Jing and Jin, Yong-Su and Rao, Christopher V.},
abstractNote = {Sugar alcohols are commonly used as low-calorie sweeteners and can serve as potential building blocks for bio-based chemicals. Previous work has shown that the oleaginous yeast Rhodosporidium toruloides IFO0880 can natively produce arabitol from xylose at relatively high titers, suggesting that it may be a useful host for sugar alcohol production. In this work, we explored whether R. toruloides can produce additional sugar alcohols. Rhodosporidium toruloides is able to produce galactitol from galactose. During growth in nitrogen-rich medium, R. toruloides produced 3.2 ± 0.6 g/L, and 8.4 ± 0.8 g/L galactitol from 20 to 40 g/L galactose, respectively. In addition, R. toruloides was able to produce galactitol from galactose at reduced titers during growth in nitrogen-poor medium, which also induces lipid production. These results suggest that R. toruloides can potentially be used for the co-production of lipids and galactitol from galactose. We further characterized the mechanism for galactitol production, including identifying and biochemically characterizing the critical aldose reductase. Intracellular metabolite analysis was also performed to further understand galactose metabolism. Rhodosporidium toruloides has traditionally been used for the production of lipids and lipid-based chemicals. Our work demonstrates that R. toruloides can also produce galactitol, which can be used to produce polymers with applications in medicine and as a precursor for anti-cancer drugs. Collectively, our results further establish that R. toruloidescan produce multiple value-added chemicals from a wide range of sugars.},
doi = {10.1186/s13068-019-1586-5},
journal = {Biotechnology for Biofuels},
number = 1,
volume = 12,
place = {Netherlands},
year = {2019},
month = {10}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
DOI: https://doi.org/10.1186/s13068-019-1586-5

Citation Metrics:
Cited by: 1 work
Citation information provided by
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Figures / Tables:

Fig. 1 Fig. 1: Growth of R. toruloides IFO0880 on different sugars at 20 g/L in nitrogen‑rich medium: a glucose, b fructose, c mannose, d sucrose, e arabinose, f glycerol, g cellobiose, and h galactose

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    Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.