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Title: Production of galactitol from galactose by the oleaginous yeast Rhodosporidium toruloides IFO0880

Abstract

Abstract Background Sugar alcohols are commonly used as low-calorie sweeteners and can serve as potential building blocks for bio-based chemicals. Previous work has shown that the oleaginous yeast Rhodosporidium toruloides IFO0880 can natively produce arabitol from xylose at relatively high titers, suggesting that it may be a useful host for sugar alcohol production. In this work, we explored whether R. toruloides can produce additional sugar alcohols. Results Rhodosporidium toruloides is able to produce galactitol from galactose. During growth in nitrogen-rich medium, R. toruloides produced 3.2 ± 0.6 g/L, and 8.4 ± 0.8 g/L galactitol from 20 to 40 g/L galactose, respectively. In addition, R. toruloides was able to produce galactitol from galactose at reduced titers during growth in nitrogen-poor medium, which also induces lipid production. These results suggest that R. toruloides can potentially be used for the co-production of lipids and galactitol from galactose. We further characterized the mechanism for galactitol production, including identifying and biochemically characterizing the critical aldose reductase. Intracellular metabolite analysis was also performed to further understand galactose metabolism. Conclusions Rhodosporidium toruloides has traditionally been used for the production of lipids and lipid-based chemicals. Our work demonstrates that R. toruloides can also produce galactitol, which can be used to produce polymers with applications inmore » medicine and as a precursor for anti-cancer drugs. Collectively, our results further establish that R. toruloides can produce multiple value-added chemicals from a wide range of sugars.« less

Authors:
; ; ; ;
Publication Date:
Research Org.:
Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), Urbana, IL (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
OSTI Identifier:
1618767
Alternate Identifier(s):
OSTI ID: 1571254
Grant/Contract Number:  
SC0018420
Resource Type:
Published Article
Journal Name:
Biotechnology for Biofuels
Additional Journal Information:
Journal Name: Biotechnology for Biofuels Journal Volume: 12 Journal Issue: 1; Journal ID: ISSN 1754-6834
Publisher:
Springer Science + Business Media
Country of Publication:
Netherlands
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Rhodosporidium toruloides, galactose, galactitol, lipid, aldose reductase, metabolite profiling

Citation Formats

Jagtap, Sujit Sadashiv, Bedekar, Ashwini Ashok, Liu, Jing-Jing, Jin, Yong-Su, and Rao, Christopher V. Production of galactitol from galactose by the oleaginous yeast Rhodosporidium toruloides IFO0880. Netherlands: N. p., 2019. Web. doi:10.1186/s13068-019-1586-5.
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Jagtap, Sujit Sadashiv, Bedekar, Ashwini Ashok, Liu, Jing-Jing, Jin, Yong-Su, & Rao, Christopher V. Production of galactitol from galactose by the oleaginous yeast Rhodosporidium toruloides IFO0880. Netherlands. https://doi.org/10.1186/s13068-019-1586-5
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Jagtap, Sujit Sadashiv, Bedekar, Ashwini Ashok, Liu, Jing-Jing, Jin, Yong-Su, and Rao, Christopher V. Fri . "Production of galactitol from galactose by the oleaginous yeast Rhodosporidium toruloides IFO0880". Netherlands. https://doi.org/10.1186/s13068-019-1586-5.
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@article{osti_1618767,
title = {Production of galactitol from galactose by the oleaginous yeast Rhodosporidium toruloides IFO0880},
author = {Jagtap, Sujit Sadashiv and Bedekar, Ashwini Ashok and Liu, Jing-Jing and Jin, Yong-Su and Rao, Christopher V.},
abstractNote = {Abstract Background Sugar alcohols are commonly used as low-calorie sweeteners and can serve as potential building blocks for bio-based chemicals. Previous work has shown that the oleaginous yeast Rhodosporidium toruloides IFO0880 can natively produce arabitol from xylose at relatively high titers, suggesting that it may be a useful host for sugar alcohol production. In this work, we explored whether R. toruloides can produce additional sugar alcohols. Results Rhodosporidium toruloides is able to produce galactitol from galactose. During growth in nitrogen-rich medium, R. toruloides produced 3.2 ± 0.6 g/L, and 8.4 ± 0.8 g/L galactitol from 20 to 40 g/L galactose, respectively. In addition, R. toruloides was able to produce galactitol from galactose at reduced titers during growth in nitrogen-poor medium, which also induces lipid production. These results suggest that R. toruloides can potentially be used for the co-production of lipids and galactitol from galactose. We further characterized the mechanism for galactitol production, including identifying and biochemically characterizing the critical aldose reductase. Intracellular metabolite analysis was also performed to further understand galactose metabolism. Conclusions Rhodosporidium toruloides has traditionally been used for the production of lipids and lipid-based chemicals. Our work demonstrates that R. toruloides can also produce galactitol, which can be used to produce polymers with applications in medicine and as a precursor for anti-cancer drugs. Collectively, our results further establish that R. toruloides can produce multiple value-added chemicals from a wide range of sugars.},
doi = {10.1186/s13068-019-1586-5},
journal = {Biotechnology for Biofuels},
number = 1,
volume = 12,
place = {Netherlands},
year = {2019},
month = {10}
}
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https://doi.org/10.1186/s13068-019-1586-5
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Figures / Tables:

Fig. 1 Fig. 1: Growth of R. toruloides IFO0880 on different sugars at 20 g/L in nitrogen‑rich medium: a glucose, b fructose, c mannose, d sucrose, e arabinose, f glycerol, g cellobiose, and h galactose
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Works referenced in this record:

Erythritol as sweetener—wherefrom and whereto?
journal, December 2017

  • Regnat, K.; Mach, R. L.; Mach-Aigner, A. R.
  • Applied Microbiology and Biotechnology, Vol. 102, Issue 2
  • DOI: 10.1007/s00253-017-8654-1

Processes for the Production of Xylitol—A Review
journal, March 2013

Yeast Carbon Catabolite Repression
journal, June 1998

Biodegradable galactitol based crosslinked polyesters for controlled release and bone tissue engineering
journal, August 2017

  • Natarajan, Janeni; Movva, Sahitya; Madras, Giridhar
  • Materials Science and Engineering: C, Vol. 77
  • DOI: 10.1016/j.msec.2017.03.160

Engineering Rhodosporidium toruloides with a membrane transporter facilitates production and separation of carotenoids and lipids in a bi-phasic culture
journal, November 2015

  • Lee, Jaslyn J. L.; Chen, Liwei; Cao, Bin
  • Applied Microbiology and Biotechnology, Vol. 100, Issue 2
  • DOI: 10.1007/s00253-015-7102-3

Identification of superior lipid producing Lipomyces and Myxozyma yeasts
journal, January 2016

  • S. Dien, Bruce; J. Slininger, Patricia; P. Kurtzman, Cletus
  • AIMS Environmental Science, Vol. 3, Issue 1
  • DOI: 10.3934/environsci.2016.1.1

Dianhydrogalactitol, a potential multitarget agent, inhibits glioblastoma migration, invasion, and angiogenesis
journal, July 2017

A survey of yeast from the Yarrowia clade for lipid production in dilute acid pretreated lignocellulosic biomass hydrolysate
journal, December 2016

  • Quarterman, Josh; Slininger, Patricia J.; Kurtzman, Cletus P.
  • Applied Microbiology and Biotechnology, Vol. 101, Issue 8
  • DOI: 10.1007/s00253-016-8062-y

Chemical Routes for the Transformation of Biomass into Chemicals
journal, June 2007

  • Corma, Avelino; Iborra, Sara; Velty, Alexandra
  • Chemical Reviews, Vol. 107, Issue 6, p. 2411-2502
  • DOI: 10.1021/cr050989d

ClustVis: a web tool for visualizing clustering of multivariate data using Principal Component Analysis and heatmap
journal, May 2015

  • Metsalu, Tauno; Vilo, Jaak
  • Nucleic Acids Research, Vol. 43, Issue W1
  • DOI: 10.1093/nar/gkv468

A Mutation in PGM2 Causing Inefficient Galactose Metabolism in the Probiotic Yeast Saccharomyces boulardii
journal, March 2018

  • Liu, Jing-Jing; Zhang, Guo-Chang; Kong, In Iok
  • Applied and Environmental Microbiology, Vol. 84, Issue 10
  • DOI: 10.1128/AEM.02858-17

Formation of Dulcitol in Aerobic Dissimilation of d-Galactose by Yeasts
journal, January 1968

Delay or Inhibition of Rat Lens Opacification using Pantethine and WR-77913
journal, January 1996

  • Clark, John I.; Livesey, John C.; Steele, Jeannette E.
  • Experimental Eye Research, Vol. 62, Issue 1
  • DOI: 10.1006/exer.1996.0009

Sugar alcohols—their role in the modern world of sweeteners: a review
journal, February 2015

Metabolic engineering of the oleaginous yeast Rhodosporidium toruloides IFO0880 for lipid overproduction during high-density fermentation
journal, September 2016

  • Zhang, Shuyan; Ito, Masakazu; Skerker, Jeffrey M.
  • Applied Microbiology and Biotechnology, Vol. 100, Issue 21
  • DOI: 10.1007/s00253-016-7815-y

Health potential of polyols as sugar replacers, with emphasis on low glycaemic properties
journal, December 2003

  • Livesey, Geoffrey
  • Nutrition Research Reviews, Vol. 16, Issue 2
  • DOI: 10.1079/NRR200371

Metabolomic Profiling of Rhodosporidium toruloides Grown on Glycerol for Carotenoid Production during Different Growth Phases
journal, October 2014

  • Lee, Jaslyn Jie Lin; Chen, Liwei; Shi, Jiahua
  • Journal of Agricultural and Food Chemistry, Vol. 62, Issue 41
  • DOI: 10.1021/jf502987q

Functional genomics of lipid metabolism in the oleaginous yeast Rhodosporidium toruloides
journal, March 2018

  • Coradetti, Samuel T.; Pinel, Dominic; Geiselman, Gina M.
  • eLife, Vol. 7
  • DOI: 10.7554/eLife.32110

Engineering Rhodosporidium toruloides for increased lipid production : Engineering
journal, November 2015

  • Zhang, Shuyan; Skerker, Jeffrey M.; Rutter, Charles D.
  • Biotechnology and Bioengineering, Vol. 113, Issue 5
  • DOI: 10.1002/bit.25864

Microbial conversion of xylose into useful bioproducts
journal, August 2018

  • Jagtap, Sujit Sadashiv; Rao, Christopher V.
  • Applied Microbiology and Biotechnology, Vol. 102, Issue 21
  • DOI: 10.1007/s00253-018-9294-9

Systematic Identification of Conserved Metabolites in GC/MS Data for Metabolomics and Biomarker Discovery
journal, February 2007

  • Styczynski, Mark P.; Moxley, Joel F.; Tong, Lily V.
  • Analytical Chemistry, Vol. 79, Issue 3
  • DOI: 10.1021/ac0614846

Purification and partial characterization of an aldo-keto reductase from Saccharomyces cerevisiae.
journal, January 1995

Anticancer activity and mechanisms of diacetyldianhydrogalactitol on hepatoma QGY-7703 cells
journal, January 2009

Culture strategies for lipid production using acetic acid as sole carbon source by Rhodosporidium toruloides
journal, April 2016

Oily yeasts as oleaginous cell factories
journal, April 2011

  • Ageitos, Jose Manuel; Vallejo, Juan Andres; Veiga-Crespo, Patricia
  • Applied Microbiology and Biotechnology, Vol. 90, Issue 4
  • DOI: 10.1007/s00253-011-3200-z

An integrated method for spectrum extraction and compound identification from gas chromatography/mass spectrometry data
journal, August 1999

The Engineering Potential of Rhodosporidium toruloides as a Workhorse for Biotechnological Applications
journal, March 2018

All in the family: aldose reductase and closely related aldo-keto reductases
journal, April 2004

Overexpression of the aldose reductase GRE 3 suppresses lithium-induced galactose toxicity in Saccharomyces cerevisiae
journal, December 2008

GMD@CSB.DB: the Golm Metabolome Database
journal, December 2004

Recent advances in biological production of erythritol
journal, September 2017

  • Rzechonek, Dorota A.; Dobrowolski, Adam; Rymowicz, Waldemar
  • Critical Reviews in Biotechnology, Vol. 38, Issue 4
  • DOI: 10.1080/07388551.2017.1380598

How Saccharomyces Responds to Nutrients
journal, December 2008

Biotechnological production of erythritol and its applications
journal, February 2010

  • Moon, Hee-Jung; Jeya, Marimuthu; Kim, In-Won
  • Applied Microbiology and Biotechnology, Vol. 86, Issue 4
  • DOI: 10.1007/s00253-010-2496-4

Production of d-arabitol from d-xylose by the oleaginous yeast Rhodosporidium toruloides IFO0880
journal, November 2017

  • Jagtap, Sujit Sadashiv; Rao, Christopher V.
  • Applied Microbiology and Biotechnology, Vol. 102, Issue 1
  • DOI: 10.1007/s00253-017-8581-1

Development of Graphene Oxide-/Galactitol Polyester-Based Biodegradable Composites for Biomedical Applications
journal, September 2017

Characterization of New Polyol/H+ Symporters in Debaryomyces hansenii
journal, February 2014

Production of d-arabitol by a newly isolated Zygosaccharomyces rouxii
journal, March 2007

  • Saha, Badal C.; Sakakibara, Yoshikiyo; Cotta, Michael A.
  • Journal of Industrial Microbiology & Biotechnology, Vol. 34, Issue 7
  • DOI: 10.1007/s10295-007-0211-y

Production of arabitol by yeasts: current status and future prospects
journal, April 2015

  • Kordowska-Wiater, M.
  • Journal of Applied Microbiology, Vol. 119, Issue 2
  • DOI: 10.1111/jam.12807

Rapid screening method for quantitation of bacterial cell lipids from whole cells
journal, November 2003

Biotransformation of lactose to galactitol
journal, January 1994

  • Muniruzzaman, Syed; Itoh, Hiromichi; Yoshino, Akira
  • Journal of Fermentation and Bioengineering, Vol. 77, Issue 1
  • DOI: 10.1016/0922-338X(94)90204-6

The roles of galactitol, galactose-1-phosphate, and phosphoglucomutase in galactose-induced toxicity in Saccharomyces cerevisiae
journal, October 2008

  • de Jongh, Willem A.; Bro, Christoffer; Ostergaard, Simon
  • Biotechnology and Bioengineering, Vol. 101, Issue 2
  • DOI: 10.1002/bit.21890

Evaluation and Optimization of Metabolome Sample Preparation Methods for Saccharomyces cerevisiae
journal, January 2013

  • Kim, Sooah; Lee, Do Yup; Wohlgemuth, Gert
  • Analytical Chemistry, Vol. 85, Issue 4
  • DOI: 10.1021/ac302881e
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