N-dependent dynamics of root growth and nitrate and ammonium uptake are altered by the bacterium Herbaspirillum seropedicae in the cereal model Brachypodium distachyon
Abstract
Nitrogen (N) fixation in cereals by root-associated bacteria is a promising solution for reducing use of chemical N fertilizers in agriculture. However, plant and bacterial responses are unpredictable across environments. We hypothesized that cereal responses to N-fixing bacteria are dynamic, depending on N supply and time. To quantify the dynamics, a gnotobiotic, fabricated ecosystem (EcoFAB) was adapted to analyse N mass balance, to image shoot and root growth, and to measure gene expression of Brachypodium distachyon inoculated with the N-fixing bacterium Herbaspirillum seropedicae. Phenotyping throughput of EcoFAB-N was 25–30 plants h-1 with open software and imaging systems. Herbaspirillum seropedicae inoculation of B. distachyon shifted root and shoot growth, nitrate versus ammonium uptake, and gene expression with time; directions and magnitude depended on N availability. Primary roots were longer and root hairs shorter regardless of N, with stronger changes at low N. At higher N, H. seropedicae provided 11% of the total plant N that came from sources other than the seed or the nutrient solution. The time-resolved phenotypic and molecular data point to distinct modes of action: at 5 mM NH4NO3 the benefit appears through N fixation, while at 0.5 mM NH4NO3 the mechanism appears to be plant physiological, withmore »
- Authors:
-
[1];
[2];
[2];
[3];
[2];
[4];
[4];
[5];
[2]
- Hunan University of Arts and Science, Changde (China); Chinese Academy of Sciences (CAS), Changsha (China)
- Forschungszentrum Jülich (Germany)
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States); USDOE Joint Genome Institute (JGI), Berkeley, CA (United States)
- Forschungszentrum Jülich (Germany); University of Melbourne, VIC (Australia)
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER); German Research Foundation (DFG); Office of the China Postdoctoral Council (OCPC); Forschungszentrum Jülich–University of Melbourne postgraduate academy (JUMPA)
- OSTI Identifier:
- 1907596
- Grant/Contract Number:
- AC02-05CH11231; 491111487; ZD2017016
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Journal of Experimental Botany
- Additional Journal Information:
- Journal Volume: 73; Journal Issue: 15; Journal ID: ISSN 0022-0957
- Publisher:
- Oxford University Press
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; agriculture; Brachypodium; cereals; crop productivity; EcoFAB; Herbaspirillum seropedicae; N fixation; nitrogen; non-invasive phenotyping; plant-growth promoting rhizobacteria; wheat
Citation Formats
Kuang, Weiqi, Sanow, Stefan, Kelm, Jana M., Müller Linow, Mark, Andeer, Peter, Kohlheyer, Dietrich, Northen, Trent, Vogel, John P., Watt, Michelle, and Arsova, Borjana. N-dependent dynamics of root growth and nitrate and ammonium uptake are altered by the bacterium Herbaspirillum seropedicae in the cereal model Brachypodium distachyon. United States: N. p., 2022.
Web. doi:10.1093/jxb/erac184.
Kuang, Weiqi, Sanow, Stefan, Kelm, Jana M., Müller Linow, Mark, Andeer, Peter, Kohlheyer, Dietrich, Northen, Trent, Vogel, John P., Watt, Michelle, & Arsova, Borjana. N-dependent dynamics of root growth and nitrate and ammonium uptake are altered by the bacterium Herbaspirillum seropedicae in the cereal model Brachypodium distachyon. United States. https://doi.org/10.1093/jxb/erac184
Kuang, Weiqi, Sanow, Stefan, Kelm, Jana M., Müller Linow, Mark, Andeer, Peter, Kohlheyer, Dietrich, Northen, Trent, Vogel, John P., Watt, Michelle, and Arsova, Borjana. Thu .
"N-dependent dynamics of root growth and nitrate and ammonium uptake are altered by the bacterium Herbaspirillum seropedicae in the cereal model Brachypodium distachyon". United States. https://doi.org/10.1093/jxb/erac184. https://www.osti.gov/servlets/purl/1907596.
@article{osti_1907596,
title = {N-dependent dynamics of root growth and nitrate and ammonium uptake are altered by the bacterium Herbaspirillum seropedicae in the cereal model Brachypodium distachyon},
author = {Kuang, Weiqi and Sanow, Stefan and Kelm, Jana M. and Müller Linow, Mark and Andeer, Peter and Kohlheyer, Dietrich and Northen, Trent and Vogel, John P. and Watt, Michelle and Arsova, Borjana},
abstractNote = {Nitrogen (N) fixation in cereals by root-associated bacteria is a promising solution for reducing use of chemical N fertilizers in agriculture. However, plant and bacterial responses are unpredictable across environments. We hypothesized that cereal responses to N-fixing bacteria are dynamic, depending on N supply and time. To quantify the dynamics, a gnotobiotic, fabricated ecosystem (EcoFAB) was adapted to analyse N mass balance, to image shoot and root growth, and to measure gene expression of Brachypodium distachyon inoculated with the N-fixing bacterium Herbaspirillum seropedicae. Phenotyping throughput of EcoFAB-N was 25–30 plants h-1 with open software and imaging systems. Herbaspirillum seropedicae inoculation of B. distachyon shifted root and shoot growth, nitrate versus ammonium uptake, and gene expression with time; directions and magnitude depended on N availability. Primary roots were longer and root hairs shorter regardless of N, with stronger changes at low N. At higher N, H. seropedicae provided 11% of the total plant N that came from sources other than the seed or the nutrient solution. The time-resolved phenotypic and molecular data point to distinct modes of action: at 5 mM NH4NO3 the benefit appears through N fixation, while at 0.5 mM NH4NO3 the mechanism appears to be plant physiological, with H. seropedicae promoting uptake of N from the root medium.Future work could fine-tune plant and root-associated microorganisms to growth and nutrient dynamics.},
doi = {10.1093/jxb/erac184},
journal = {Journal of Experimental Botany},
number = 15,
volume = 73,
place = {United States},
year = {Thu May 05 00:00:00 EDT 2022},
month = {Thu May 05 00:00:00 EDT 2022}
}
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