A Chemical-Free Pretreatment for Biosynthesis of Bioethanol and Lipids from Lignocellulosic Biomass: An Industrially Relevant 2G Biorefinery Approach

Deshavath, Narendra Naik; Dien, Bruce S.; Slininger, Patricia J.; Jin, Yong-Su; Singh, Vijay

doi:10.3390/fermentation9010005

Title: A Chemical-Free Pretreatment for Biosynthesis of Bioethanol and Lipids from Lignocellulosic Biomass: An Industrially Relevant 2G Biorefinery Approach

Abstract

A wide range of inorganic and organic chemicals are used during the pretreatment and enzymatic hydrolysis of lignocellulosic biomass to produce biofuels. Developing an industrially relevant 2G biorefinery process using such chemicals is challenging and requires more unit operations for downstream processing. A sustainable process has been developed to achieve industrially relevant titers of bioethanol with significant ethanol yield. The pretreatment of sorghum biomass was performed by a continuous pilot-scale hydrothermal reactor followed by disk milling. Enzymatic hydrolysis was performed without washing the pretreated biomass. Moreover, citrate buffer strength was reduced to 100-fold (50 mM to 0.5 mM) during the enzymatic hydrolysis. Enzymatic hydrolysis at 0.5 mM citrate buffer strength showed that significant sugar concentrations of 222 ± 2.3 to 241 ± 2.3 g/L (glucose + xylose) were attained at higher solids loadings of 50 to 60% (w/v). Furthermore, hydrolysates were fermented to produce bioethanol using two different xylose-fermenting Saccharomyces cerevisiae strains and a co-culture of xylose-fermenting and non-GMO yeast cultures. Bioethanol titer of 81.7 g/L was achieved with an ethanol yield of 0.48 gp/gs. Additionally, lipids were produced using the oleaginous yeast Rhodosporidium toruloides, yielding 13.2 g/L lipids with cellular lipid accumulation of 38.5% w/w from 100 g/L ofmore »« less

Authors:

Deshavath, Narendra Naik; Dien, Bruce S.; Slininger, Patricia J.; Jin, Yong-Su;

Publication Date:: Wed Dec 21 00:00:00 EST 2022

Sponsoring Org.:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

OSTI Identifier:: 1906403

Grant/Contract Number:: SC0018420

Resource Type:: Published Article

Journal Name:: Fermentation

Additional Journal Information:: Journal Name: Fermentation Journal Volume: 9 Journal Issue: 1; Journal ID: ISSN 2311-5637

Publisher:: MDPI AG

Country of Publication:: Switzerland

Language:: English

Citation Formats


                    Deshavath, Narendra Naik, Dien, Bruce S., Slininger, Patricia J., Jin, Yong-Su, and Singh, Vijay. A Chemical-Free Pretreatment for Biosynthesis of Bioethanol and Lipids from Lignocellulosic Biomass: An Industrially Relevant 2G Biorefinery Approach.  Switzerland: N. p., 2022. 
Web.  doi:10.3390/fermentation9010005.

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                    Deshavath, Narendra Naik, Dien, Bruce S., Slininger, Patricia J., Jin, Yong-Su, & Singh, Vijay. A Chemical-Free Pretreatment for Biosynthesis of Bioethanol and Lipids from Lignocellulosic Biomass: An Industrially Relevant 2G Biorefinery Approach.  Switzerland.  https://doi.org/10.3390/fermentation9010005

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                    Deshavath, Narendra Naik, Dien, Bruce S., Slininger, Patricia J., Jin, Yong-Su, and Singh, Vijay. Wed .  
"A Chemical-Free Pretreatment for Biosynthesis of Bioethanol and Lipids from Lignocellulosic Biomass: An Industrially Relevant 2G Biorefinery Approach".  Switzerland.  https://doi.org/10.3390/fermentation9010005.

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@article{osti_1906403,

  title        = {A Chemical-Free Pretreatment for Biosynthesis of Bioethanol and Lipids from Lignocellulosic Biomass: An Industrially Relevant 2G Biorefinery Approach},

  author       = {Deshavath, Narendra Naik and Dien, Bruce S. and Slininger, Patricia J. and Jin, Yong-Su and Singh, Vijay},

  abstractNote = {A wide range of inorganic and organic chemicals are used during the pretreatment and enzymatic hydrolysis of lignocellulosic biomass to produce biofuels. Developing an industrially relevant 2G biorefinery process using such chemicals is challenging and requires more unit operations for downstream processing. A sustainable process has been developed to achieve industrially relevant titers of bioethanol with significant ethanol yield. The pretreatment of sorghum biomass was performed by a continuous pilot-scale hydrothermal reactor followed by disk milling. Enzymatic hydrolysis was performed without washing the pretreated biomass. Moreover, citrate buffer strength was reduced to 100-fold (50 mM to 0.5 mM) during the enzymatic hydrolysis. Enzymatic hydrolysis at 0.5 mM citrate buffer strength showed that significant sugar concentrations of 222 ± 2.3 to 241 ± 2.3 g/L (glucose + xylose) were attained at higher solids loadings of 50 to 60% (w/v). Furthermore, hydrolysates were fermented to produce bioethanol using two different xylose-fermenting Saccharomyces cerevisiae strains and a co-culture of xylose-fermenting and non-GMO yeast cultures. Bioethanol titer of 81.7 g/L was achieved with an ethanol yield of 0.48 gp/gs. Additionally, lipids were produced using the oleaginous yeast Rhodosporidium toruloides, yielding 13.2 g/L lipids with cellular lipid accumulation of 38.5% w/w from 100 g/L of sugar concentration. In summary, reducing the strength of the citrate buffer during enzymatic hydrolysis and omitting inorganic chemicals from the pretreatment process enhances the fermentability of hydrolysates and can also reduce operating costs.},

  doi          = {10.3390/fermentation9010005},

  journal      = {Fermentation},

  number       = 1,

  volume       = 9,

  place        = {Switzerland},

  year         = {Wed Dec 21 00:00:00 EST 2022},

  month        = {Wed Dec 21 00:00:00 EST 2022}

}

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Title: A Chemical-Free Pretreatment for Biosynthesis of Bioethanol and Lipids from Lignocellulosic Biomass: An Industrially Relevant 2G Biorefinery Approach

Abstract

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Conversion of High-Solids Hydrothermally Pretreated Bioenergy Sorghum to Lipids and Ethanol Using Yeast Cultures
journal, June 2021

Development of dilute sulfuric acid pretreatment method for the enhancement of xylose fermentability
journal, July 2017

Promise of combined hydrothermal/chemical and mechanical refining for pretreatment of woody and herbaceous biomass
journal, April 2016

Sugar production from bioenergy sorghum by using pilot scale continuous hydrothermal pretreatment combined with disk refining
journal, October 2019

Production of fuels and chemicals from xylose by engineered Saccharomyces cerevisiae: a review and perspective
journal, May 2017

Understanding the Pine Dilute Acid Pretreatment System for Enhanced Enzymatic Hydrolysis
journal, September 2015

A novel integrated biological process for cellulosic ethanol production featuring high ethanol productivity, enzyme recycling and yeast cells reuse
journal, January 2012

Adaptive response of yeasts to furfural and 5-hydroxymethylfurfural and new chemical evidence for HMF conversion to 2,5-bis-hydroxymethylfuran
journal, July 2004

Exploitation of genus Rhodosporidium for microbial lipid production
journal, February 2017

Chemical composition analysis of various genetically modified sorghum traits: Pretreatment process optimization and bioethanol production from hemicellulosic hydrolyzates without detoxification
journal, August 2018

Effect of acetic acid and pH on the cofermentation of glucose and xylose to ethanol by a genetically engineered strain of Saccharomyces cerevisiae: Effect of acetic acid/pH on xylose fermentation
journal, March 2010

Phenolic Amides Are Potent Inhibitors of De Novo Nucleotide Biosynthesis
journal, June 2015

Hydrothermal hydrolysis of microcrystalline cellulose from birch wood catalyzed by Al2O3-B2O3 mixed oxides
journal, March 2022

Identification of superior lipid producing Lipomyces and Myxozyma yeasts
journal, January 2016

Liquefaction of lignocellulosic biomass through biochemical conversion pathway: A strategic approach to achieve an industrial titer of bioethanol
journal, March 2021

Enhanced lipid production by Rhodosporidium toruloides using different fed-batch feeding strategies with lignocellulosic hydrolysate as the sole carbon source
journal, June 2016

Fermentation technology to improve productivity in dry grind corn process for bioethanol production
journal, May 2018

Robust paths to net greenhouse gas mitigation and negative emissions via advanced biofuels
journal, August 2020

Comparative lipid production by oleaginous yeasts in hydrolyzates of lignocellulosic biomass and process strategy for high titers: High Titer Lipid Production by Yeasts in Lignocellulosic Hydrolyzates
journal, February 2016

Features of promising technologies for pretreatment of lignocellulosic biomass
journal, April 2005

Process Design and Economics for Biochemical Conversion of Lignocellulosic Biomass to Ethanol: Dilute-Acid Pretreatment and Enzymatic Hydrolysis of Corn Stover
report, March 2011

Effects of hydrothermal pretreatment on the dissolution and structural evolution of hemicelluloses and lignin: A review
journal, April 2022

High titer cellulosic ethanol production from sugarcane bagasse via DLCA pretreatment and process development without washing/detoxifying pretreated biomass
journal, March 2022

Microbial production of advanced biofuels
journal, June 2021

High solids loading biorefinery for the production of cellulosic sugars from bioenergy sorghum
journal, December 2020

Thermogravimetric–mass spectrometric analysis of lignocellulosic and marine biomass pyrolysis
journal, April 2012

DMR (deacetylation and mechanical refining) processing of corn stover achieves high monomeric sugar concentrations (230 g L ⁻¹ ) during enzymatic hydrolysis and high ethanol concentrations (>10% v/v) during fermentation without hydrolysate purification or concentration
journal, January 2016

Oily yeasts as oleaginous cell factories
journal, April 2011

Curtailing citrate buffer inhibition effect on S. cerevisiae to enhance the fermentability of cellulosic hydrolysate
journal, August 2021

Modified simultaneous saccharification and co-fermentation of DLC pretreated corn stover for high-titer cellulosic ethanol production without water washing or detoxifying pretreated biomass
journal, May 2022

How Pseudo-lignin Is Generated during Dilute Sulfuric Acid Pretreatment
journal, August 2019

Increasing ethanol productivity during xylose fermentation by cell recycling of recombinant Saccharomyces cerevisiae
journal, January 2003

Controlled feeding of cellulases improves conversion of xylose in simultaneous saccharification and co-fermentation for bioethanol production
journal, January 2010

Cellulosic ethanol production from AFEX-treated corn stover using Saccharomyces cerevisiae 424A(LNH-ST)
journal, January 2009

Repression of xylose-specific enzymes by ethanol in Scheffersomyces (Pichia) stipitis and utility of repitching xylose-grown populations to eliminate diauxic lag
journal, March 2011

Lignocellulose pretreatment technologies affect the level of enzymatic cellulose oxidation by LPMO
journal, January 2015

Inhibition Performance of Lignocellulose Degradation Products on Industrial Cellulase Enzymes During Cellulose Hydrolysis
journal, January 2009

Improved bioconversion of lignocellulosic biomass bySaccharomyces cerevisiaeengineered for tolerance to acetic acid
journal, November 2019

Kinetic studies on glucose and xylose transport in Saccharomyces cerevisiae
journal, October 2002

Dry biorefining maximizes the potentials of simultaneous saccharification and co-fermentation for cellulosic ethanol production
journal, October 2017

Production of xylose enriched hydrolysate from bioenergy sorghum and its conversion to β-carotene using an engineered Saccharomyces cerevisiae
journal, July 2020

Effect of salts on the Co-fermentation of glucose and xylose by a genetically engineered strain of Saccharomyces cerevisiae
journal, January 2013

Rapid and marker-free refactoring of xylose-fermenting yeast strains with Cas9/CRISPR: Rapid and Marker-Free Cas9/CRISPR Refactoring
journal, June 2015