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Title: Reconstructed Ancestral Myo-Inositol-3-Phosphate Synthases Indicate That Ancestors of the Thermococcales and Thermotoga Species Were More Thermophilic than Their Descendants

Abstract

The bacterial genomes of Thermotoga species show evidence of significant interdomain horizontal gene transfer from the Archaea. Members of this genus acquired many genes from the Thermococcales, which grow at higher temperatures than Thermotoga species. In order to study the functional history of an interdomain horizontally acquired gene we used ancestral sequence reconstruction to examine the thermal characteristics of reconstructed ancestral proteins of the Thermotoga lineage and its archaeal donors. Several ancestral sequence reconstruction methods were used to determine the possible sequences of the ancestral Thermotoga and Archaea myo-inositol-3-phosphate synthase (MIPS). These sequences were predicted to be more thermostable than the extant proteins using an established sequence composition method. We verified these computational predictions by measuring the activities and thermostabilities of purified proteins from the Thermotoga and the Thermococcales species, and eight ancestral reconstructed proteins. We found that the ancestral proteins from both the archaeal donor and the Thermotoga most recent common ancestor recipient were more thermostable than their descendants. We show that there is a correlation between the thermostability of MIPS protein and the optimal growth temperature (OGT) of its host, which suggests that the OGT of the ancestors of these species of Archaea and the Thermotoga grew atmore » higher OGTs than their descendants.« less

Authors:
 [1];  [1];  [1];  [1];  [1];  [1]
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  1. University of Connecticut, Storrs, CT (United States)
Publication Date:
Research Org.:
Univ. of Connecticut, Storrs, CT (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER); National Aeronautics and Space Administration (NASA); National Science Foundation (NSF)
OSTI Identifier:
1904029
Grant/Contract Number:  
PS02-08ER08-12; NNX08AQ10G; DEB0830024
Resource Type:
Accepted Manuscript
Journal Name:
PLoS ONE
Additional Journal Information:
Journal Volume: 8; Journal Issue: 12; Journal ID: ISSN 1932-6203
Publisher:
Public Library of Science
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; thermotoga; archaea; phylogenetic analysis; protein sequencing; pyrococcus; trees; amino acid sequence analysis; phylogenetics

Citation Formats

Butzin, Nicholas C., Lapierre, Pascal, Green, Anna G., Swithers, Kristen S., Gogarten, J. Peter, and Noll, Kenneth M. Reconstructed Ancestral Myo-Inositol-3-Phosphate Synthases Indicate That Ancestors of the Thermococcales and Thermotoga Species Were More Thermophilic than Their Descendants. United States: N. p., 2013. Web. doi:10.1371/journal.pone.0084300.
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Butzin, Nicholas C., Lapierre, Pascal, Green, Anna G., Swithers, Kristen S., Gogarten, J. Peter, & Noll, Kenneth M. Reconstructed Ancestral Myo-Inositol-3-Phosphate Synthases Indicate That Ancestors of the Thermococcales and Thermotoga Species Were More Thermophilic than Their Descendants. United States. https://doi.org/10.1371/journal.pone.0084300
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Butzin, Nicholas C., Lapierre, Pascal, Green, Anna G., Swithers, Kristen S., Gogarten, J. Peter, and Noll, Kenneth M. Tue . "Reconstructed Ancestral Myo-Inositol-3-Phosphate Synthases Indicate That Ancestors of the Thermococcales and Thermotoga Species Were More Thermophilic than Their Descendants". United States. https://doi.org/10.1371/journal.pone.0084300. https://www.osti.gov/servlets/purl/1904029.
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@article{osti_1904029,
title = {Reconstructed Ancestral Myo-Inositol-3-Phosphate Synthases Indicate That Ancestors of the Thermococcales and Thermotoga Species Were More Thermophilic than Their Descendants},
author = {Butzin, Nicholas C. and Lapierre, Pascal and Green, Anna G. and Swithers, Kristen S. and Gogarten, J. Peter and Noll, Kenneth M.},
abstractNote = {The bacterial genomes of Thermotoga species show evidence of significant interdomain horizontal gene transfer from the Archaea. Members of this genus acquired many genes from the Thermococcales, which grow at higher temperatures than Thermotoga species. In order to study the functional history of an interdomain horizontally acquired gene we used ancestral sequence reconstruction to examine the thermal characteristics of reconstructed ancestral proteins of the Thermotoga lineage and its archaeal donors. Several ancestral sequence reconstruction methods were used to determine the possible sequences of the ancestral Thermotoga and Archaea myo-inositol-3-phosphate synthase (MIPS). These sequences were predicted to be more thermostable than the extant proteins using an established sequence composition method. We verified these computational predictions by measuring the activities and thermostabilities of purified proteins from the Thermotoga and the Thermococcales species, and eight ancestral reconstructed proteins. We found that the ancestral proteins from both the archaeal donor and the Thermotoga most recent common ancestor recipient were more thermostable than their descendants. We show that there is a correlation between the thermostability of MIPS protein and the optimal growth temperature (OGT) of its host, which suggests that the OGT of the ancestors of these species of Archaea and the Thermotoga grew at higher OGTs than their descendants.},
doi = {10.1371/journal.pone.0084300},
journal = {PLoS ONE},
number = 12,
volume = 8,
place = {United States},
year = {Tue Dec 31 00:00:00 EST 2013},
month = {Tue Dec 31 00:00:00 EST 2013}
}
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    Inferring Orthologs: Open Questions and Perspectives
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