Re-Programing Glucose Catabolism in the Microalga Chlorella sorokiniana under Light Condition
Abstract
The microalga Chlorella sorokiniana has attracted much attention for lipid production and wastewater treatment. It can perform photosynthesis and organic carbon utilization concurrently. To understand its phototrophic metabolism, a biomass compositional analysis, a 13C metabolic flux analysis, and metabolite pool size analyses were performed. Under dark condition, the oxidative pentose phosphate pathway (OPP) was the major route for glucose catabolism (88% carbon flux) and a cyclic OPP–glycolytic route for glucose catabolism was formed. Under light condition, fluxes in the glucose catabolism, tricarboxylic acid (TCA) cycle, and anaplerotic reaction (CO2 fixation via phosphoenolpyruvate carboxylase) were all suppressed. Meanwhile, the RuBisCO reaction became active and the ratio of its carbon fixation to glucose carbon utilization was determined as 7:100. Moreover, light condition significantly reduced the pool sizes of sugar phosphate metabolites (such as E4P, F6P, and S7P) and promoted biomass synthesis (which reached 0.155 h–1). In addition, light condition increased glucose consumption rates, leading to higher ATP and NADPH production and a higher protein content (43% vs. 30%) in the biomass during the exponential growth phase.
- Authors:
-
[4];
[3];
- Lanzhou Univ. (China); Washington State Univ., Pullman, WA (United States)
- Washington State Univ., Pullman, WA (United States); Michigan State Univ., East Lansing, MI (United States)
- Washington Univ., St. Louis, MO (United States)
- Michigan State Univ., East Lansing, MI (United States)
- Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Laboratory
- Washington State Univ., Pullman, WA (United States)
- Publication Date:
- Research Org.:
- Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Laboratory
- Sponsoring Org.:
- USDOE; USDA
- OSTI Identifier:
- 1903887
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Biomolecules
- Additional Journal Information:
- Journal Volume: 12; Journal Issue: 7; Journal ID: ISSN 2218-273X
- Publisher:
- MDPI
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; microalgae; Chlorella sorokiniana; 13C-MFA; mixotrophic; heterotrophic; pool size
Citation Formats
Li, Tingting, Pang, Na, He, Lian, Xu, Yuan, Fu, Xinyu, Tang, Yinjie J., Shachar-Hill, Yair, and Chen, Shulin. Re-Programing Glucose Catabolism in the Microalga Chlorella sorokiniana under Light Condition. United States: N. p., 2022.
Web. doi:10.3390/biom12070939.
Li, Tingting, Pang, Na, He, Lian, Xu, Yuan, Fu, Xinyu, Tang, Yinjie J., Shachar-Hill, Yair, & Chen, Shulin. Re-Programing Glucose Catabolism in the Microalga Chlorella sorokiniana under Light Condition. United States. https://doi.org/10.3390/biom12070939
Li, Tingting, Pang, Na, He, Lian, Xu, Yuan, Fu, Xinyu, Tang, Yinjie J., Shachar-Hill, Yair, and Chen, Shulin. Mon .
"Re-Programing Glucose Catabolism in the Microalga Chlorella sorokiniana under Light Condition". United States. https://doi.org/10.3390/biom12070939. https://www.osti.gov/servlets/purl/1903887.
@article{osti_1903887,
title = {Re-Programing Glucose Catabolism in the Microalga Chlorella sorokiniana under Light Condition},
author = {Li, Tingting and Pang, Na and He, Lian and Xu, Yuan and Fu, Xinyu and Tang, Yinjie J. and Shachar-Hill, Yair and Chen, Shulin},
abstractNote = {The microalga Chlorella sorokiniana has attracted much attention for lipid production and wastewater treatment. It can perform photosynthesis and organic carbon utilization concurrently. To understand its phototrophic metabolism, a biomass compositional analysis, a 13C metabolic flux analysis, and metabolite pool size analyses were performed. Under dark condition, the oxidative pentose phosphate pathway (OPP) was the major route for glucose catabolism (88% carbon flux) and a cyclic OPP–glycolytic route for glucose catabolism was formed. Under light condition, fluxes in the glucose catabolism, tricarboxylic acid (TCA) cycle, and anaplerotic reaction (CO2 fixation via phosphoenolpyruvate carboxylase) were all suppressed. Meanwhile, the RuBisCO reaction became active and the ratio of its carbon fixation to glucose carbon utilization was determined as 7:100. Moreover, light condition significantly reduced the pool sizes of sugar phosphate metabolites (such as E4P, F6P, and S7P) and promoted biomass synthesis (which reached 0.155 h–1). In addition, light condition increased glucose consumption rates, leading to higher ATP and NADPH production and a higher protein content (43% vs. 30%) in the biomass during the exponential growth phase.},
doi = {10.3390/biom12070939},
journal = {Biomolecules},
number = 7,
volume = 12,
place = {United States},
year = {Mon Jul 04 00:00:00 EDT 2022},
month = {Mon Jul 04 00:00:00 EDT 2022}
}
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