Harvesting the Microalga Chlorella sorokiniana by Fungal-Assisted Pelletization

Toscano, Lydia; Ogden, Kimberly L.; Ogden, Greg; Cervantes, Lourdes; Steichen, Seth A.; Brown, Caitlin; Samaniego, Blancka Y.; Brown, Judith K.

doi:10.1166/jbmb.2018.1798

Title: Harvesting the Microalga Chlorella sorokiniana by Fungal-Assisted Pelletization

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Abstract

Currently, the co-cultivation of fungal and microalgal cells is considered a potential technology to minimize the harvesting cost and energy inputs in the algae-to-biofuel approach. In the present study, the co-cultivation of five selected filamentous fungi and microalgae Chlorella sorokiniana to form cell pellets was evaluated under different conditions, such as organic carbon (glucose, fructose, sucrose, and maltose) and nitrogen (urea, peptone, yeast extract, and sodium nitrate) source content with and without the presence of an alternative carbon source (wheat straw), and initial fungal/microalgae cells ratio for mixotrophic growing. In addition, cellulase activity and zeta potential measurements were carried out to obtain a better understanding of the pelletization process. Aspergillus flavus was found most efficient for pelletizing in the nutrient supplemented spent BG-11 in spite of its alkaline pH (7.5–8.3) under mixotrophic growing, being able to harvest up to 96% of the microalgal cells (4.2 x 10⁷ cells mL^-1 initial algal concentration) within 48 h at supplementation of 5, 10, and 1 g L^-1 of fructose, NaNO₃, and KH₂PO₄, respectively. The harvesting efficiency in terms of optical density at 750 nm as well as the variation of residual sugars, pH, and cellulase enzyme activity (2.3 U mL^-1 maximum value) withmore »« less

Authors:

Toscano, Lydia ^[1]; Ogden, Kimberly L. ^[2]; Ogden, Greg ^[2]; Cervantes, Lourdes ^[2]; Steichen, Seth A. ^[2]; Brown, Caitlin ^[3]; Samaniego, Blancka Y. ^[2]; Brown, Judith K. ^[4]

Technological Inst. of Mexicali (Mexico)
Univ. of Arizona, Tucson, AZ (United States)
Autonomous Univ. of Baja California (Mexico)
Technological Inst. of Conkal (Mexico)

Publication Date:: Sat Dec 01 00:00:00 EST 2018

Research Org.:: Univ. of Arizona, Tucson, AZ (United States)

Sponsoring Org.:: USDOE Office of Energy Efficiency and Renewable Energy (EERE), Sustainable Transportation Office. Bioenergy Technologies Office

OSTI Identifier:: 1581802

Grant/Contract Number:: ee0006269

Resource Type:: Accepted Manuscript

Journal Name:: Journal of Biobased Materials and Bioenergy

Additional Journal Information:: Journal Volume: 12; Journal Issue: 6; Journal ID: ISSN 1556-6560

Publisher:: American Scientific Publishers

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; co-culture; microalgae harvest; filamentous fungi; pelletization

Citation Formats


                    Toscano, Lydia, Ogden, Kimberly L., Ogden, Greg, Cervantes, Lourdes, Steichen, Seth A., Brown, Caitlin, Samaniego, Blancka Y., and Brown, Judith K. Harvesting the Microalga Chlorella sorokiniana by Fungal-Assisted Pelletization.  United States: N. p., 2018. 
Web.  doi:10.1166/jbmb.2018.1798.

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                    Toscano, Lydia, Ogden, Kimberly L., Ogden, Greg, Cervantes, Lourdes, Steichen, Seth A., Brown, Caitlin, Samaniego, Blancka Y., & Brown, Judith K. Harvesting the Microalga Chlorella sorokiniana by Fungal-Assisted Pelletization.  United States.  https://doi.org/10.1166/jbmb.2018.1798

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                    Toscano, Lydia, Ogden, Kimberly L., Ogden, Greg, Cervantes, Lourdes, Steichen, Seth A., Brown, Caitlin, Samaniego, Blancka Y., and Brown, Judith K. Sat .  
"Harvesting the Microalga Chlorella sorokiniana by Fungal-Assisted Pelletization".  United States.  https://doi.org/10.1166/jbmb.2018.1798.  https://www.osti.gov/servlets/purl/1581802.

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@article{osti_1581802,

  title        = {Harvesting the Microalga Chlorella sorokiniana by Fungal-Assisted Pelletization},

  author       = {Toscano, Lydia and Ogden, Kimberly L. and Ogden, Greg and Cervantes, Lourdes and Steichen, Seth A. and Brown, Caitlin and Samaniego, Blancka Y. and Brown, Judith K.},

  abstractNote = {Currently, the co-cultivation of fungal and microalgal cells is considered a potential technology to minimize the harvesting cost and energy inputs in the algae-to-biofuel approach. In the present study, the co-cultivation of five selected filamentous fungi and microalgae Chlorella sorokiniana to form cell pellets was evaluated under different conditions, such as organic carbon (glucose, fructose, sucrose, and maltose) and nitrogen (urea, peptone, yeast extract, and sodium nitrate) source content with and without the presence of an alternative carbon source (wheat straw), and initial fungal/microalgae cells ratio for mixotrophic growing. In addition, cellulase activity and zeta potential measurements were carried out to obtain a better understanding of the pelletization process. Aspergillus flavus was found most efficient for pelletizing in the nutrient supplemented spent BG-11 in spite of its alkaline pH (7.5–8.3) under mixotrophic growing, being able to harvest up to 96% of the microalgal cells (4.2 x 107 cells mL-1 initial algal concentration) within 48 h at supplementation of 5, 10, and 1 g L-1 of fructose, NaNO3, and KH2PO4, respectively. The harvesting efficiency in terms of optical density at 750 nm as well as the variation of residual sugars, pH, and cellulase enzyme activity (2.3 U mL-1 maximum value) with time was also studied. A. flavus strain investigated in this work could emerge as an efficient and economically viable method in microalgae harvesting for biofuel production as well as in the production of other valuable compounds from extracellular fungal metabolites.},

  doi          = {10.1166/jbmb.2018.1798},

  journal      = {Journal of Biobased Materials and Bioenergy},

  number       = 6,

  volume       = 12,

  place        = {United States},

  year         = {Sat Dec 01 00:00:00 EST 2018},

  month        = {Sat Dec 01 00:00:00 EST 2018}

}

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