Experimental evidence for the impact of soil viruses on carbon cycling during surface plant litter decomposition
Abstract
To date, the potential impact of viral communities on biogeochemical cycles in soil has largely been inferred from correlational evidence, such as virus-driven changes in microbial abundances, viral auxiliary metabolic genes, and links with soil physiochemical properties. To more directly test the impact of soil viruses on carbon cycling during plant litter decomposition, we added concentrated viral community suspensions to complex litter decomposer communities in 40-day microcosm experiments. Microbial communities from two New Mexico alpine soils, Pajarito (PJ) and Santa Fe (SF), were inoculated onto grass litter on sand, and three treatments were applied in triplicate to each set of microcosms: addition of buffer (no added virus), live virus (+virus), or killed-virus (+killed-virus) fractions extracted from the same soil. Significant differences in respiration were observed between the +virus and +killed-virus treatments in the PJ, but not the SF microcosms. Bacterial and fungal community composition differed significantly by treatment in both PJ and SF microcosms. Combining data across both soils, viral addition altered links between bacterial and fungal diversity, dissolved organic carbon and total nitrogen. Overall, we demonstrate that increasing viral pressure in complex microbial communities can impact terrestrial biogeochemical cycling but is context-dependent.
- Publication Date:
- Research Org.:
- Los Alamos National Laboratory (LANL), Los Alamos, NM (United States)
- Sponsoring Org.:
- USDOE Laboratory Directed Research and Development (LDRD) Program; USDOE Office of Science (SC), Biological and Environmental Research (BER); USDOE National Nuclear Security Administration (NNSA)
- OSTI Identifier:
- 1855309
- Alternate Identifier(s):
- OSTI ID: 2008271
- Report Number(s):
- LA-UR-21-28749
Journal ID: ISSN 2730-6151; 24
- Grant/Contract Number:
- 89233218CNA000001; 20200252ER; 2018LANLF255; SC0021198
- Resource Type:
- Published Article
- Journal Name:
- ISME Communications
- Additional Journal Information:
- Journal Name: ISME Communications Journal Volume: 2 Journal Issue: 1; Journal ID: ISSN 2730-6151
- Publisher:
- Springer Nature
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 54 ENVIRONMENTAL SCIENCES
Citation Formats
Albright, Michaeline B. N., Gallegos-Graves, La Verne, Feeser, Kelli L., Montoya, Kyana, Emerson, Joanne B., Shakya, Migun, and Dunbar, John. Experimental evidence for the impact of soil viruses on carbon cycling during surface plant litter decomposition. United States: N. p., 2022.
Web. doi:10.1038/s43705-022-00109-4.
Albright, Michaeline B. N., Gallegos-Graves, La Verne, Feeser, Kelli L., Montoya, Kyana, Emerson, Joanne B., Shakya, Migun, & Dunbar, John. Experimental evidence for the impact of soil viruses on carbon cycling during surface plant litter decomposition. United States. https://doi.org/10.1038/s43705-022-00109-4
Albright, Michaeline B. N., Gallegos-Graves, La Verne, Feeser, Kelli L., Montoya, Kyana, Emerson, Joanne B., Shakya, Migun, and Dunbar, John. Wed .
"Experimental evidence for the impact of soil viruses on carbon cycling during surface plant litter decomposition". United States. https://doi.org/10.1038/s43705-022-00109-4.
@article{osti_1855309,
title = {Experimental evidence for the impact of soil viruses on carbon cycling during surface plant litter decomposition},
author = {Albright, Michaeline B. N. and Gallegos-Graves, La Verne and Feeser, Kelli L. and Montoya, Kyana and Emerson, Joanne B. and Shakya, Migun and Dunbar, John},
abstractNote = {To date, the potential impact of viral communities on biogeochemical cycles in soil has largely been inferred from correlational evidence, such as virus-driven changes in microbial abundances, viral auxiliary metabolic genes, and links with soil physiochemical properties. To more directly test the impact of soil viruses on carbon cycling during plant litter decomposition, we added concentrated viral community suspensions to complex litter decomposer communities in 40-day microcosm experiments. Microbial communities from two New Mexico alpine soils, Pajarito (PJ) and Santa Fe (SF), were inoculated onto grass litter on sand, and three treatments were applied in triplicate to each set of microcosms: addition of buffer (no added virus), live virus (+virus), or killed-virus (+killed-virus) fractions extracted from the same soil. Significant differences in respiration were observed between the +virus and +killed-virus treatments in the PJ, but not the SF microcosms. Bacterial and fungal community composition differed significantly by treatment in both PJ and SF microcosms. Combining data across both soils, viral addition altered links between bacterial and fungal diversity, dissolved organic carbon and total nitrogen. Overall, we demonstrate that increasing viral pressure in complex microbial communities can impact terrestrial biogeochemical cycling but is context-dependent.},
doi = {10.1038/s43705-022-00109-4},
journal = {ISME Communications},
number = 1,
volume = 2,
place = {United States},
year = {Wed Mar 16 00:00:00 EDT 2022},
month = {Wed Mar 16 00:00:00 EDT 2022}
}
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