Microbial Communities Influence Soil Dissolved Organic Carbon Concentration by Altering Metabolite Composition
Abstract
Rapid microbial growth in the early phase of plant litter decomposition is viewed as an important component of soil organic matter (SOM) formation. However, the microbial taxa and chemical substrates that correlate with carbon storage are not well resolved. The complexity of microbial communities and diverse substrate chemistries that occur in natural soils make it difficult to identify links between community membership and decomposition processes in the soil environment. To identify potential relationships between microbes, soil organic matter, and their impact on carbon storage, we used sand microcosms to control for external environmental factors such as changes in temperature and moisture as well as the variability in available carbon that exist in soil cores. Using Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS) on microcosm samples from early phase litter decomposition, we found that protein- and tannin-like compounds exhibited the strongest correlation to dissolved organic carbon (DOC) concentration. Proteins correlated positively with DOC concentration, while tannins correlated negatively with DOC. Through random forest, neural network, and indicator species analyses, we identified 42 bacterial and 9 fungal taxa associated with DOC concentration. The majority of bacterial taxa (26 out of 42 taxa) belonged to the phylum Proteobacteria while all fungal taxamore »
- Authors:
- Publication Date:
- Research Org.:
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States); Los Alamos National Lab. (LANL), Los Alamos, NM (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1841317
- Alternate Identifier(s):
- OSTI ID: 1855403
- Report Number(s):
- PNNL-SA-167395
Journal ID: ISSN 1664-302X; 799014
- Grant/Contract Number:
- 2015SFAF260; 2019SFAF255; EMSL project 49834; AC05-76RL01830
- Resource Type:
- Published Article
- Journal Name:
- Frontiers in Microbiology
- Additional Journal Information:
- Journal Name: Frontiers in Microbiology Journal Volume: 12; Journal ID: ISSN 1664-302X
- Publisher:
- Frontiers Media SA
- Country of Publication:
- Switzerland
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; DOC; microbial communities; FTICR mass spectrometry; metabolites; bacteria; fungi
Citation Formats
Campbell, Tayte P., Ulrich, Danielle E. M., Toyoda, Jason, Thompson, Jaron, Munsky, Brian, Albright, Michaeline B. N., Bailey, Vanessa L., Tfaily, Malak M., and Dunbar, John. Microbial Communities Influence Soil Dissolved Organic Carbon Concentration by Altering Metabolite Composition. Switzerland: N. p., 2022.
Web. doi:10.3389/fmicb.2021.799014.
Campbell, Tayte P., Ulrich, Danielle E. M., Toyoda, Jason, Thompson, Jaron, Munsky, Brian, Albright, Michaeline B. N., Bailey, Vanessa L., Tfaily, Malak M., & Dunbar, John. Microbial Communities Influence Soil Dissolved Organic Carbon Concentration by Altering Metabolite Composition. Switzerland. https://doi.org/10.3389/fmicb.2021.799014
Campbell, Tayte P., Ulrich, Danielle E. M., Toyoda, Jason, Thompson, Jaron, Munsky, Brian, Albright, Michaeline B. N., Bailey, Vanessa L., Tfaily, Malak M., and Dunbar, John. Thu .
"Microbial Communities Influence Soil Dissolved Organic Carbon Concentration by Altering Metabolite Composition". Switzerland. https://doi.org/10.3389/fmicb.2021.799014.
@article{osti_1841317,
title = {Microbial Communities Influence Soil Dissolved Organic Carbon Concentration by Altering Metabolite Composition},
author = {Campbell, Tayte P. and Ulrich, Danielle E. M. and Toyoda, Jason and Thompson, Jaron and Munsky, Brian and Albright, Michaeline B. N. and Bailey, Vanessa L. and Tfaily, Malak M. and Dunbar, John},
abstractNote = {Rapid microbial growth in the early phase of plant litter decomposition is viewed as an important component of soil organic matter (SOM) formation. However, the microbial taxa and chemical substrates that correlate with carbon storage are not well resolved. The complexity of microbial communities and diverse substrate chemistries that occur in natural soils make it difficult to identify links between community membership and decomposition processes in the soil environment. To identify potential relationships between microbes, soil organic matter, and their impact on carbon storage, we used sand microcosms to control for external environmental factors such as changes in temperature and moisture as well as the variability in available carbon that exist in soil cores. Using Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS) on microcosm samples from early phase litter decomposition, we found that protein- and tannin-like compounds exhibited the strongest correlation to dissolved organic carbon (DOC) concentration. Proteins correlated positively with DOC concentration, while tannins correlated negatively with DOC. Through random forest, neural network, and indicator species analyses, we identified 42 bacterial and 9 fungal taxa associated with DOC concentration. The majority of bacterial taxa (26 out of 42 taxa) belonged to the phylum Proteobacteria while all fungal taxa belonged to the phylum Ascomycota. Additionally, we identified significant connections between microorganisms and protein-like compounds and found that most taxa (12/14) correlated negatively with proteins indicating that microbial consumption of proteins is likely a significant driver of DOC concentration. This research links DOC concentration with microbial production and/or decomposition of specific metabolites to improve our understanding of microbial metabolism and carbon persistence.},
doi = {10.3389/fmicb.2021.799014},
journal = {Frontiers in Microbiology},
number = ,
volume = 12,
place = {Switzerland},
year = {Thu Jan 20 00:00:00 EST 2022},
month = {Thu Jan 20 00:00:00 EST 2022}
}
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