Respiratory Vinyl Chloride Reductive Dechlorination to Ethene in TceA-Expressing Dehalococcoides mccartyi
Abstract
Bioremediation of chlorinated ethenes in anoxic aquifers hinges on organohalide-respiring Dehalococcoidia expressing vinyl chloride (VC) reductive dehalogenase (RDase). The tceA gene encoding the trichloroethene-dechlorinating RDase TceA is frequently detected in contaminated groundwater but not recognized as a biomarker for VC detoxification. We demonstrate that tceA-carrying Dehalococcoides mccartyi (Dhc) strains FL2 and 195 grow with VC as an electron acceptor when sufficient vitamin B12 (B12) is provided. Strain FL2 cultures that received 50 μg L–1 B12 completely dechlorinated VC to ethene at rates of 14.80 ± 1.30 μM day–1 and attained 1.64 ± 0.11 × 108 cells per μmol of VC consumed. Strain 195 attained similar growth yields of 1.80 ± 1.00 × 108 cells per μmol of VC consumed, and both strains could be consecutively transferred with VC as the electron acceptor. Proteomic analysis demonstrated TceA expression in VC-grown strain FL2 cultures. Resequencing of the strain FL2 and strain 195 tceA genes identified non-synonymous substitutions, although their consequences for TceA function are currently unknown. The finding that Dhc strains expressing TceA respire VC can explain ethene formation at chlorinated solvent sites, where quantitative polymerase chain reaction analysis indicates that tceA dominates the RDase gene pool.
- Authors:
-
[4];
[3];
[6]
- Chinese Academy of Sciences (CAS), Shenyang (China); Univ. of Tennessee, Knoxville, TN (United States)
- Chinese Academy of Sciences (CAS), Shenyang (China); University of Chinese Academy of Sciences, Beijing (China)
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Chinese Academy of Sciences (CAS), Shenyang (China)
- Univ. of Tennessee, Knoxville, TN (United States)
- Univ. of Tennessee, Knoxville, TN (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Publication Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE; National Institute of Environmental Health Sciences; National Natural Science Foundation of China (NSFC)
- OSTI Identifier:
- 1830109
- Grant/Contract Number:
- AC05-00OR22725; R01ES024294; 41673126; 41977295; 41907287
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Environmental Science and Technology
- Additional Journal Information:
- Journal Volume: 55; Journal Issue: 8; Journal ID: ISSN 0013-936X
- Publisher:
- American Chemical Society (ACS)
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 54 ENVIRONMENTAL SCIENCES; Peptides and proteins; Reductive dechlorination; Genetics; Genomics; Groundwaters
Citation Formats
Yan, Jun, Wang, Jingjing, Villalobos Solis, Manuel I., Jin, Huijuan, Chourey, Karuna, Li, Xiuying, Yang, Yi, Yin, Yongchao, Hettich, Robert L., and Löffler, Frank E. Respiratory Vinyl Chloride Reductive Dechlorination to Ethene in TceA-Expressing Dehalococcoides mccartyi. United States: N. p., 2021.
Web. doi:10.1021/acs.est.0c07354.
Yan, Jun, Wang, Jingjing, Villalobos Solis, Manuel I., Jin, Huijuan, Chourey, Karuna, Li, Xiuying, Yang, Yi, Yin, Yongchao, Hettich, Robert L., & Löffler, Frank E. Respiratory Vinyl Chloride Reductive Dechlorination to Ethene in TceA-Expressing Dehalococcoides mccartyi. United States. https://doi.org/10.1021/acs.est.0c07354
Yan, Jun, Wang, Jingjing, Villalobos Solis, Manuel I., Jin, Huijuan, Chourey, Karuna, Li, Xiuying, Yang, Yi, Yin, Yongchao, Hettich, Robert L., and Löffler, Frank E. Mon .
"Respiratory Vinyl Chloride Reductive Dechlorination to Ethene in TceA-Expressing Dehalococcoides mccartyi". United States. https://doi.org/10.1021/acs.est.0c07354. https://www.osti.gov/servlets/purl/1830109.
@article{osti_1830109,
title = {Respiratory Vinyl Chloride Reductive Dechlorination to Ethene in TceA-Expressing Dehalococcoides mccartyi},
author = {Yan, Jun and Wang, Jingjing and Villalobos Solis, Manuel I. and Jin, Huijuan and Chourey, Karuna and Li, Xiuying and Yang, Yi and Yin, Yongchao and Hettich, Robert L. and Löffler, Frank E.},
abstractNote = {Bioremediation of chlorinated ethenes in anoxic aquifers hinges on organohalide-respiring Dehalococcoidia expressing vinyl chloride (VC) reductive dehalogenase (RDase). The tceA gene encoding the trichloroethene-dechlorinating RDase TceA is frequently detected in contaminated groundwater but not recognized as a biomarker for VC detoxification. We demonstrate that tceA-carrying Dehalococcoides mccartyi (Dhc) strains FL2 and 195 grow with VC as an electron acceptor when sufficient vitamin B12 (B12) is provided. Strain FL2 cultures that received 50 μg L–1 B12 completely dechlorinated VC to ethene at rates of 14.80 ± 1.30 μM day–1 and attained 1.64 ± 0.11 × 108 cells per μmol of VC consumed. Strain 195 attained similar growth yields of 1.80 ± 1.00 × 108 cells per μmol of VC consumed, and both strains could be consecutively transferred with VC as the electron acceptor. Proteomic analysis demonstrated TceA expression in VC-grown strain FL2 cultures. Resequencing of the strain FL2 and strain 195 tceA genes identified non-synonymous substitutions, although their consequences for TceA function are currently unknown. The finding that Dhc strains expressing TceA respire VC can explain ethene formation at chlorinated solvent sites, where quantitative polymerase chain reaction analysis indicates that tceA dominates the RDase gene pool.},
doi = {10.1021/acs.est.0c07354},
journal = {Environmental Science and Technology},
number = 8,
volume = 55,
place = {United States},
year = {Mon Mar 08 00:00:00 EST 2021},
month = {Mon Mar 08 00:00:00 EST 2021}
}
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