Complete and efficient conversion of plant cell wall hemicellulose into high-value bioproducts by engineered yeast
Abstract
Abstract Plant cell wall hydrolysates contain not only sugars but also substantial amounts of acetate, a fermentation inhibitor that hinders bioconversion of lignocellulose. Despite the toxic and non-consumable nature of acetate during glucose metabolism, we demonstrate that acetate can be rapidly co-consumed with xylose by engineered Saccharomyces cerevisiae . The co-consumption leads to a metabolic re-configuration that boosts the synthesis of acetyl-CoA derived bioproducts, including triacetic acid lactone (TAL) and vitamin A, in engineered strains. Notably, by co-feeding xylose and acetate, an enginered strain produces 23.91 g/L TAL with a productivity of 0.29 g/L/h in bioreactor fermentation. This strain also completely converts a hemicellulose hydrolysate of switchgrass into 3.55 g/L TAL. These findings establish a versatile strategy that not only transforms an inhibitor into a valuable substrate but also expands the capacity of acetyl-CoA supply in S. cerevisiae for efficient bioconversion of cellulosic biomass.
- Publication Date:
- Research Org.:
- Univ. of Illinois at Urbana-Champaign, IL (United States). Center for Advanced Bioenergy and Bioproducts Innovation (CABBI)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1813624
- Alternate Identifier(s):
- OSTI ID: 1834391
- Grant/Contract Number:
- SC0018420
- Resource Type:
- Published Article
- Journal Name:
- Nature Communications
- Additional Journal Information:
- Journal Name: Nature Communications Journal Volume: 12 Journal Issue: 1; Journal ID: ISSN 2041-1723
- Publisher:
- Nature Publishing Group
- Country of Publication:
- United Kingdom
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Xylose; acetate; hemicellulose; acetyl-CoA; triacetic acid lactone; vitamin A; Saccharomyces cerevisiae
Citation Formats
Sun, Liang, Lee, Jae Won, Yook, Sangdo, Lane, Stephan, Sun, Ziqiao, Kim, Soo Rin, and Jin, Yong-Su. Complete and efficient conversion of plant cell wall hemicellulose into high-value bioproducts by engineered yeast. United Kingdom: N. p., 2021.
Web. doi:10.1038/s41467-021-25241-y.
Sun, Liang, Lee, Jae Won, Yook, Sangdo, Lane, Stephan, Sun, Ziqiao, Kim, Soo Rin, & Jin, Yong-Su. Complete and efficient conversion of plant cell wall hemicellulose into high-value bioproducts by engineered yeast. United Kingdom. https://doi.org/10.1038/s41467-021-25241-y
Sun, Liang, Lee, Jae Won, Yook, Sangdo, Lane, Stephan, Sun, Ziqiao, Kim, Soo Rin, and Jin, Yong-Su. Tue .
"Complete and efficient conversion of plant cell wall hemicellulose into high-value bioproducts by engineered yeast". United Kingdom. https://doi.org/10.1038/s41467-021-25241-y.
@article{osti_1813624,
title = {Complete and efficient conversion of plant cell wall hemicellulose into high-value bioproducts by engineered yeast},
author = {Sun, Liang and Lee, Jae Won and Yook, Sangdo and Lane, Stephan and Sun, Ziqiao and Kim, Soo Rin and Jin, Yong-Su},
abstractNote = {Abstract Plant cell wall hydrolysates contain not only sugars but also substantial amounts of acetate, a fermentation inhibitor that hinders bioconversion of lignocellulose. Despite the toxic and non-consumable nature of acetate during glucose metabolism, we demonstrate that acetate can be rapidly co-consumed with xylose by engineered Saccharomyces cerevisiae . The co-consumption leads to a metabolic re-configuration that boosts the synthesis of acetyl-CoA derived bioproducts, including triacetic acid lactone (TAL) and vitamin A, in engineered strains. Notably, by co-feeding xylose and acetate, an enginered strain produces 23.91 g/L TAL with a productivity of 0.29 g/L/h in bioreactor fermentation. This strain also completely converts a hemicellulose hydrolysate of switchgrass into 3.55 g/L TAL. These findings establish a versatile strategy that not only transforms an inhibitor into a valuable substrate but also expands the capacity of acetyl-CoA supply in S. cerevisiae for efficient bioconversion of cellulosic biomass.},
doi = {10.1038/s41467-021-25241-y},
journal = {Nature Communications},
number = 1,
volume = 12,
place = {United Kingdom},
year = {Tue Aug 17 00:00:00 EDT 2021},
month = {Tue Aug 17 00:00:00 EDT 2021}
}
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