Towards engineering ectomycorrhization into switchgrass bioenergy crops via a lectin receptor‐like kinase
Abstract
Summary Soil‐borne microbes can establish compatible relationships with host plants, providing a large variety of nutritive and protective compounds in exchange for photosynthesized sugars. However, the molecular mechanisms mediating the establishment of these beneficial relationships remain unclear. Our previous genetic mapping and whole‐genome resequencing studies identified a gene deletion event of a Populus trichocarpa lectin receptor‐like kinase gene PtLecRLK1 in Populus deltoides that was associated with poor‐root colonization by the ectomycorrhizal fungus Laccaria bicolor . By introducing PtLecRLK1 into a perennial grass known to be a non‐host of L. bicolor , switchgrass ( Panicum virgatum L.), we found that L. bicolor colonizes ZmUbipro‐PtLecRLK1 transgenic switchgrass roots, which illustrates that the introduction of PtLecRLK1 has the potential to convert a non‐host to a host of L. bicolor . Furthermore, transcriptomic and proteomic analyses on inoculated‐transgenic switchgrass roots revealed genes/proteins overrepresented in the compatible interaction and underrepresented in the pathogenic defence pathway, consistent with the view that pathogenic defence response is down‐regulated during compatible interaction. Metabolomic profiling revealed that root colonization in the transgenic switchgrass was associated with an increase in N‐containing metabolites and a decrease in organic acids, sugars, and aromatic hydroxycinnamate conjugates, which are often seen in the early stepsmore »
- Authors:
-
[1];
[1];
[1]
- Biosciences Division Oak Ridge National Laboratory Oak Ridge TN USA
- Biosciences Division Oak Ridge National Laboratory Oak Ridge TN USA, Bredesen Center for Interdisciplinary Research and Graduate Education University of Tennessee Knoxville TN USA
- Genome Science and Technology University of Tennessee Knoxville TN USA, Chemical Science Division Oak Ridge National Laboratory Oak Ridge TN USA
- Noble Research Institute Ardmore OK USA
- Biosciences Division Oak Ridge National Laboratory Oak Ridge TN USA, Chemical Science Division Oak Ridge National Laboratory Oak Ridge TN USA
- Publication Date:
- Research Org.:
- Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1812816
- Alternate Identifier(s):
- OSTI ID: 1811404; OSTI ID: 1812817
- Grant/Contract Number:
- AC05-00OR22725
- Resource Type:
- Published Article
- Journal Name:
- Plant Biotechnology Journal
- Additional Journal Information:
- Journal Name: Plant Biotechnology Journal Journal Volume: 19 Journal Issue: 12; Journal ID: ISSN 1467-7644
- Publisher:
- Wiley-Blackwell
- Country of Publication:
- United Kingdom
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; PtLecRLK1; ectomycorrhizal symbiosis; Laccaria bicolor; switchgrass; Panicum virgatum
Citation Formats
Qiao, Zhenzhen, Yates, Timothy B., Shrestha, Him K., Engle, Nancy L., Flanagan, Amy, Morrell‐Falvey, Jennifer L., Sun, Yali, Tschaplinski, Timothy J., Abraham, Paul E., Labbé, Jessy, Wang, Zeng‐Yu, Hettich, Robert L., Tuskan, Gerald A., Muchero, Wellington, and Chen, Jin‐Gui. Towards engineering ectomycorrhization into switchgrass bioenergy crops via a lectin receptor‐like kinase. United Kingdom: N. p., 2021.
Web. doi:10.1111/pbi.13671.
Qiao, Zhenzhen, Yates, Timothy B., Shrestha, Him K., Engle, Nancy L., Flanagan, Amy, Morrell‐Falvey, Jennifer L., Sun, Yali, Tschaplinski, Timothy J., Abraham, Paul E., Labbé, Jessy, Wang, Zeng‐Yu, Hettich, Robert L., Tuskan, Gerald A., Muchero, Wellington, & Chen, Jin‐Gui. Towards engineering ectomycorrhization into switchgrass bioenergy crops via a lectin receptor‐like kinase. United Kingdom. https://doi.org/10.1111/pbi.13671
Qiao, Zhenzhen, Yates, Timothy B., Shrestha, Him K., Engle, Nancy L., Flanagan, Amy, Morrell‐Falvey, Jennifer L., Sun, Yali, Tschaplinski, Timothy J., Abraham, Paul E., Labbé, Jessy, Wang, Zeng‐Yu, Hettich, Robert L., Tuskan, Gerald A., Muchero, Wellington, and Chen, Jin‐Gui. Wed .
"Towards engineering ectomycorrhization into switchgrass bioenergy crops via a lectin receptor‐like kinase". United Kingdom. https://doi.org/10.1111/pbi.13671.
@article{osti_1812816,
title = {Towards engineering ectomycorrhization into switchgrass bioenergy crops via a lectin receptor‐like kinase},
author = {Qiao, Zhenzhen and Yates, Timothy B. and Shrestha, Him K. and Engle, Nancy L. and Flanagan, Amy and Morrell‐Falvey, Jennifer L. and Sun, Yali and Tschaplinski, Timothy J. and Abraham, Paul E. and Labbé, Jessy and Wang, Zeng‐Yu and Hettich, Robert L. and Tuskan, Gerald A. and Muchero, Wellington and Chen, Jin‐Gui},
abstractNote = {Summary Soil‐borne microbes can establish compatible relationships with host plants, providing a large variety of nutritive and protective compounds in exchange for photosynthesized sugars. However, the molecular mechanisms mediating the establishment of these beneficial relationships remain unclear. Our previous genetic mapping and whole‐genome resequencing studies identified a gene deletion event of a Populus trichocarpa lectin receptor‐like kinase gene PtLecRLK1 in Populus deltoides that was associated with poor‐root colonization by the ectomycorrhizal fungus Laccaria bicolor . By introducing PtLecRLK1 into a perennial grass known to be a non‐host of L. bicolor , switchgrass ( Panicum virgatum L.), we found that L. bicolor colonizes ZmUbipro‐PtLecRLK1 transgenic switchgrass roots, which illustrates that the introduction of PtLecRLK1 has the potential to convert a non‐host to a host of L. bicolor . Furthermore, transcriptomic and proteomic analyses on inoculated‐transgenic switchgrass roots revealed genes/proteins overrepresented in the compatible interaction and underrepresented in the pathogenic defence pathway, consistent with the view that pathogenic defence response is down‐regulated during compatible interaction. Metabolomic profiling revealed that root colonization in the transgenic switchgrass was associated with an increase in N‐containing metabolites and a decrease in organic acids, sugars, and aromatic hydroxycinnamate conjugates, which are often seen in the early steps of establishing compatible interactions. These studies illustrate that PtLecRLK1 is able to render a plant susceptible to colonization by the ectomycorrhizal fungus L. bicolor and shed light on engineering mycorrhizal symbiosis into a non‐host to enhance plant productivity and fitness on marginal lands.},
doi = {10.1111/pbi.13671},
journal = {Plant Biotechnology Journal},
number = 12,
volume = 19,
place = {United Kingdom},
year = {Wed Aug 11 00:00:00 EDT 2021},
month = {Wed Aug 11 00:00:00 EDT 2021}
}
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