Intra‐species genetic variability drives carbon metabolism and symbiotic host interactions in the ectomycorrhizal fungus Pisolithus microcarpus
Abstract
Summary Ectomycorrhizal (ECM) fungi are integral to boreal and temperate forest ecosystem functioning and nutrient cycling. ECM fungi, however, originate from diverse saprotrophic lineages and the impacts of genetic variation across species, and especially within a given ECM species, on function and interactions with the environment is not well understood. Here, we explore the extent of intra‐species variation between four isolates of the ECM fungus Pisolithus microcarpus , in terms of gene regulation, carbon metabolism and growth, and interactions with a host, Eucalyptus grandis . We demonstrate that, while a core response to the host is maintained by all of the isolates tested, they have distinct patterns of gene expression and carbon metabolism, resulting in the differential expression of isolate‐specific response pathways in the host plant. Together, these results highlight the importance of using a wider range of individuals within a species to understand the broader ecological roles of ECM fungi and their host interactions.
- Authors:
-
[1];
[2];
[4];
[4];
[4];
[5];
[2];
[1];
[1]
- Hawkesbury Institute for the Environment Western Sydney University Richmond NSW 2753 Australia
- Université de Lorraine, INRAE, UMR Interactions Arbres/Microorganismes, Laboratory of Excellence ARBRE, INRAE GrandEst‐Nancy Champenoux 54280 France
- Royal Botanic Gardens Victoria Melbourne VIC 3004 Australia, Botanic Gardens and State Herbarium of South Australia Adelaide SA 5000 Australia
- US Department of Energy Joint Genome Institute Lawrence Berkeley National Laboratory Berkeley CA 94720 USA
- US Department of Energy Joint Genome Institute Lawrence Berkeley National Laboratory Berkeley CA 94720 USA, Department of Plant and Microbial Biology University of California Berkeley CA 94720 USA
- Publication Date:
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1804926
- Resource Type:
- Publisher's Accepted Manuscript
- Journal Name:
- Environmental Microbiology
- Additional Journal Information:
- Journal Name: Environmental Microbiology Journal Volume: 23 Journal Issue: 4; Journal ID: ISSN 1462-2912
- Publisher:
- Wiley-Blackwell
- Country of Publication:
- United Kingdom
- Language:
- English
Citation Formats
Plett, Krista L., Kohler, Annegret, Lebel, Teresa, Singan, Vasanth R., Bauer, Diane, He, Guifen, Ng, Vivian, Grigoriev, Igor V., Martin, Francis, Plett, Jonathan M., and Anderson, Ian C. Intra‐species genetic variability drives carbon metabolism and symbiotic host interactions in the ectomycorrhizal fungus Pisolithus microcarpus. United Kingdom: N. p., 2020.
Web. doi:10.1111/1462-2920.15320.
Plett, Krista L., Kohler, Annegret, Lebel, Teresa, Singan, Vasanth R., Bauer, Diane, He, Guifen, Ng, Vivian, Grigoriev, Igor V., Martin, Francis, Plett, Jonathan M., & Anderson, Ian C. Intra‐species genetic variability drives carbon metabolism and symbiotic host interactions in the ectomycorrhizal fungus Pisolithus microcarpus. United Kingdom. https://doi.org/10.1111/1462-2920.15320
Plett, Krista L., Kohler, Annegret, Lebel, Teresa, Singan, Vasanth R., Bauer, Diane, He, Guifen, Ng, Vivian, Grigoriev, Igor V., Martin, Francis, Plett, Jonathan M., and Anderson, Ian C. Wed .
"Intra‐species genetic variability drives carbon metabolism and symbiotic host interactions in the ectomycorrhizal fungus Pisolithus microcarpus". United Kingdom. https://doi.org/10.1111/1462-2920.15320.
@article{osti_1804926,
title = {Intra‐species genetic variability drives carbon metabolism and symbiotic host interactions in the ectomycorrhizal fungus Pisolithus microcarpus},
author = {Plett, Krista L. and Kohler, Annegret and Lebel, Teresa and Singan, Vasanth R. and Bauer, Diane and He, Guifen and Ng, Vivian and Grigoriev, Igor V. and Martin, Francis and Plett, Jonathan M. and Anderson, Ian C.},
abstractNote = {Summary Ectomycorrhizal (ECM) fungi are integral to boreal and temperate forest ecosystem functioning and nutrient cycling. ECM fungi, however, originate from diverse saprotrophic lineages and the impacts of genetic variation across species, and especially within a given ECM species, on function and interactions with the environment is not well understood. Here, we explore the extent of intra‐species variation between four isolates of the ECM fungus Pisolithus microcarpus , in terms of gene regulation, carbon metabolism and growth, and interactions with a host, Eucalyptus grandis . We demonstrate that, while a core response to the host is maintained by all of the isolates tested, they have distinct patterns of gene expression and carbon metabolism, resulting in the differential expression of isolate‐specific response pathways in the host plant. Together, these results highlight the importance of using a wider range of individuals within a species to understand the broader ecological roles of ECM fungi and their host interactions.},
doi = {10.1111/1462-2920.15320},
journal = {Environmental Microbiology},
number = 4,
volume = 23,
place = {United Kingdom},
year = {Wed Nov 18 00:00:00 EST 2020},
month = {Wed Nov 18 00:00:00 EST 2020}
}
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