Inorganic nitrogen availability alters Eucalyptus grandis receptivity to the ectomycorrhizal fungus Pisolithus albus but not symbiotic nitrogen transfer
Abstract
Forest trees are able to thrive in nutrient-poor soils in part because they obtain growth-limiting nutrients, especially nitrogen (N), through mutualistic symbiosis with ectomycorrhizal (ECM) fungi. Addition of inorganic N into these soils is known to disrupt this mutualism and reduce the diversity of ECM fungi. Despite its ecological impact, the mechanisms governing the observed effects of elevated inorganic N on mycorrhizal communities remain unknown. Here, we address this by using a compartmentalized in vitro system to independently alter nutrients to each symbiont. Using stable isotopes, we traced the nutrient flux under different nutrient regimes between Eucalyptus grandis and its ectomycorrhizal symbiont, Pisolithus albus. We demonstrate that giving E. grandis independent access to N causes a significant reduction in root colonization by P. albus. Transcriptional analysis suggests that the observed reduction in colonization may be caused, in part, by altered transcription of microbe perception genes and defence genes. We show that delivery of N to host leaves is not increased by host nutrient deficiency but by fungal nutrient availability instead. Overall, this advances our understanding of the effects of N fertilization on ECM fungi and the factors governing nutrient transfer in the E. grandis-P. microcarpus interaction.
- Authors:
-
[1];
[2];
[2];
[2];
[2];
[3];
[1];
- Western Sydney Univ., Richmond, NSW (Australia)
- USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
- INRA, Champenoux (France)
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC); Australian Research Council
- OSTI Identifier:
- 1619172
- Alternate Identifier(s):
- OSTI ID: 1579597
- Grant/Contract Number:
- AC02-05CH11231; DP160102684; CSP1953; AC02‐05CH11231
- Resource Type:
- Accepted Manuscript
- Journal Name:
- New Phytologist
- Additional Journal Information:
- Journal Volume: 226; Journal Issue: 1; Journal ID: ISSN 0028-646X
- Publisher:
- Wiley
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; ectomycorrhizal (ECM) fungi; nitrogen deposition; nutrient trading; stable isotope tracing; transcriptomic analysis
Citation Formats
Plett, Krista L., Singan, Vasanth R., Wang, Mei, Ng, Vivian, Grigoriev, Igor V., Martin, Francis, Plett, Jonathan M., and Anderson, Ian C. Inorganic nitrogen availability alters Eucalyptus grandis receptivity to the ectomycorrhizal fungus Pisolithus albus but not symbiotic nitrogen transfer. United States: N. p., 2019.
Web. doi:10.1111/nph.16322.
Plett, Krista L., Singan, Vasanth R., Wang, Mei, Ng, Vivian, Grigoriev, Igor V., Martin, Francis, Plett, Jonathan M., & Anderson, Ian C. Inorganic nitrogen availability alters Eucalyptus grandis receptivity to the ectomycorrhizal fungus Pisolithus albus but not symbiotic nitrogen transfer. United States. https://doi.org/10.1111/nph.16322
Plett, Krista L., Singan, Vasanth R., Wang, Mei, Ng, Vivian, Grigoriev, Igor V., Martin, Francis, Plett, Jonathan M., and Anderson, Ian C. Fri .
"Inorganic nitrogen availability alters Eucalyptus grandis receptivity to the ectomycorrhizal fungus Pisolithus albus but not symbiotic nitrogen transfer". United States. https://doi.org/10.1111/nph.16322. https://www.osti.gov/servlets/purl/1619172.
@article{osti_1619172,
title = {Inorganic nitrogen availability alters Eucalyptus grandis receptivity to the ectomycorrhizal fungus Pisolithus albus but not symbiotic nitrogen transfer},
author = {Plett, Krista L. and Singan, Vasanth R. and Wang, Mei and Ng, Vivian and Grigoriev, Igor V. and Martin, Francis and Plett, Jonathan M. and Anderson, Ian C.},
abstractNote = {Forest trees are able to thrive in nutrient-poor soils in part because they obtain growth-limiting nutrients, especially nitrogen (N), through mutualistic symbiosis with ectomycorrhizal (ECM) fungi. Addition of inorganic N into these soils is known to disrupt this mutualism and reduce the diversity of ECM fungi. Despite its ecological impact, the mechanisms governing the observed effects of elevated inorganic N on mycorrhizal communities remain unknown. Here, we address this by using a compartmentalized in vitro system to independently alter nutrients to each symbiont. Using stable isotopes, we traced the nutrient flux under different nutrient regimes between Eucalyptus grandis and its ectomycorrhizal symbiont, Pisolithus albus. We demonstrate that giving E. grandis independent access to N causes a significant reduction in root colonization by P. albus. Transcriptional analysis suggests that the observed reduction in colonization may be caused, in part, by altered transcription of microbe perception genes and defence genes. We show that delivery of N to host leaves is not increased by host nutrient deficiency but by fungal nutrient availability instead. Overall, this advances our understanding of the effects of N fertilization on ECM fungi and the factors governing nutrient transfer in the E. grandis-P. microcarpus interaction.},
doi = {10.1111/nph.16322},
journal = {New Phytologist},
number = 1,
volume = 226,
place = {United States},
year = {Fri Nov 15 00:00:00 EST 2019},
month = {Fri Nov 15 00:00:00 EST 2019}
}
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