Heterogeneous Distribution of Erucic Acid in Brassica napus Seeds
Abstract
Brassica napus (B. napus) is the world's most widely grown temperate oilseed crop. Although breeding for human consumption has led to removal of erucic acid from refined canola oils, there is renewed interest in the industrial uses of erucic acid derived from B. napus, and there is a rich germplasm available for use. Here, low- and high-erucic acid accessions of B. napus seeds were examined for the distribution of erucic acid-containing lipids and the gene transcripts encoding the enzymes involved in pathways for its incorporation into triacylglycerols (TAGs) across the major tissues of the seeds. In general, the results indicate that a heterogeneous distribution of erucic acid across B. napus seed tissues was contributed by two isoforms (out of six) of FATTY ACYL COA ELONGASE (FAE1) and a combination of phospholipid:diacylglycerol acyltransferase (PDAT)- and diacylglycerol acyltransferase (DGAT)-mediated incorporation of erucic acid into TAGs in cotyledonary tissues. An absence of the expression of these two FAE1 isoforms accounted for the absence of erucic acid in the TAGs of the lowerucic accession.
- Authors:
-
- Huazhong Agricultural Univ., Wuhan (China). National Key Lab. of Crop Genetic Improvement
- Univ. of North Texas, Denton, TX (United States). Dept. of Biological Sciences. Center for Plant Lipid Research; Univ. of North Texas, Denton, TX (United States). BioDiscovery Inst.
- Univ. of North Texas, Denton, TX (United States). Dept. of Biological Sciences. Center for Plant Lipid Research; Univ. of North Texas, Denton, TX (United States). BioDiscovery Inst.; Univ. of Texas Southwestern Medical Center, Dallas, TX (United States)
- Publication Date:
- Research Org.:
- Univ. of North Texas, Denton, TX (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1803957
- Grant/Contract Number:
- SC0020325
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Frontiers in Plant Science
- Additional Journal Information:
- Journal Volume: 10; Journal ID: ISSN 1664-462X
- Publisher:
- Frontiers Research Foundation
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Plant Sciences; Brassica napus; canola; erucic acid; spatial distribution; matrix assisted laser desorption/ionizationmass spectrometry imaging
Citation Formats
Lu, Shaoping, Aziz, Mina, Sturtevant, Drew, Chapman, Kent D., and Guo, Liang. Heterogeneous Distribution of Erucic Acid in Brassica napus Seeds. United States: N. p., 2020.
Web. doi:10.3389/fpls.2019.01744.
Lu, Shaoping, Aziz, Mina, Sturtevant, Drew, Chapman, Kent D., & Guo, Liang. Heterogeneous Distribution of Erucic Acid in Brassica napus Seeds. United States. https://doi.org/10.3389/fpls.2019.01744
Lu, Shaoping, Aziz, Mina, Sturtevant, Drew, Chapman, Kent D., and Guo, Liang. Wed .
"Heterogeneous Distribution of Erucic Acid in Brassica napus Seeds". United States. https://doi.org/10.3389/fpls.2019.01744. https://www.osti.gov/servlets/purl/1803957.
@article{osti_1803957,
title = {Heterogeneous Distribution of Erucic Acid in Brassica napus Seeds},
author = {Lu, Shaoping and Aziz, Mina and Sturtevant, Drew and Chapman, Kent D. and Guo, Liang},
abstractNote = {Brassica napus (B. napus) is the world's most widely grown temperate oilseed crop. Although breeding for human consumption has led to removal of erucic acid from refined canola oils, there is renewed interest in the industrial uses of erucic acid derived from B. napus, and there is a rich germplasm available for use. Here, low- and high-erucic acid accessions of B. napus seeds were examined for the distribution of erucic acid-containing lipids and the gene transcripts encoding the enzymes involved in pathways for its incorporation into triacylglycerols (TAGs) across the major tissues of the seeds. In general, the results indicate that a heterogeneous distribution of erucic acid across B. napus seed tissues was contributed by two isoforms (out of six) of FATTY ACYL COA ELONGASE (FAE1) and a combination of phospholipid:diacylglycerol acyltransferase (PDAT)- and diacylglycerol acyltransferase (DGAT)-mediated incorporation of erucic acid into TAGs in cotyledonary tissues. An absence of the expression of these two FAE1 isoforms accounted for the absence of erucic acid in the TAGs of the lowerucic accession.},
doi = {10.3389/fpls.2019.01744},
journal = {Frontiers in Plant Science},
number = ,
volume = 10,
place = {United States},
year = {Wed Jan 29 00:00:00 EST 2020},
month = {Wed Jan 29 00:00:00 EST 2020}
}
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Modified oleic cottonseeds show altered content, composition and tissue-specific distribution of triacylglycerol molecular species
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A jojoba beta-Ketoacyl-CoA synthase cDNA complements the canola fatty acid elongation mutation in transgenic plants.
journal, February 1996
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Development of ultra-high erucic acid oil in the industrial oil crop Crambe abyssinica : Development of ultra-high erucic acid oil
journal, May 2012
- Li, Xueyuan; van Loo, Eibertus N.; Gruber, Jens
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A novel quantitative trait locus on chromosome A9 controlling oleic acid content in Brassica napus
journal, May 2019
- Zhao, Qing; Wu, Jian; Cai, Guangqin
- Plant Biotechnology Journal, Vol. 17, Issue 12
The impact of reducing fatty acid desaturation on the composition and thermal stability of rapeseed oil
journal, October 2019
- Kaur, Harjeevan; Wang, Lihong; Stawniak, Natalia
- Plant Biotechnology Journal, Vol. 18, Issue 4
Imaging heterogeneity of membrane and storage lipids in transgenic Camelina sativa seeds with altered fatty acid profiles
journal, July 2013
- Horn, Patrick J.; Silva, Jillian E.; Anderson, Danielle
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Quantitative profiling and pattern analysis of triacylglycerol species in Arabidopsis seeds by electrospray ionization mass spectrometry
journal, November 2013
- Li, Maoyin; Baughman, Ethan; Roth, Mary R.
- The Plant Journal, Vol. 77, Issue 1
Spatial analysis of lipid metabolites and expressed genes reveals tissue-specific heterogeneity of lipid metabolism in high- and low-oil Brassica napus L. seeds
journal, June 2018
- Lu, Shaoping; Sturtevant, Drew; Aziz, Mina
- The Plant Journal, Vol. 94, Issue 6
Characterization of FAE1 in the zero erucic acid germplasm of Brassica rapa L.
journal, January 2015
- Yan, Guixin; Li, Dan; Cai, Mengxian
- Breeding Science, Vol. 65, Issue 3
Effect of replacing solvent-extracted canola meal with high-oil traditional canola, high-oleic acid canola, or high-erucic acid rapeseed meals on rumen fermentation, digestibility, milk production, and milk fatty acid composition in lactating dairy cows
journal, August 2011
- Hristov, A. N.; Domitrovich, C.; Wachter, A.
- Journal of Dairy Science, Vol. 94, Issue 8