Heterogeneous Distribution of Erucic Acid in Brassica napus Seeds

Lu, Shaoping; Aziz, Mina; Sturtevant, Drew; Chapman, Kent D.; Guo, Liang

doi:10.3389/fpls.2019.01744

Title: Heterogeneous Distribution of Erucic Acid in Brassica napus Seeds

Journal Article · 28 January 2020 · Frontiers in Plant Science

DOI: https://doi.org/10.3389/fpls.2019.01744 · OSTI ID:1803957

Lu, Shaoping ^[1]; Aziz, Mina ^[2]; Sturtevant, Drew ^[3]; Chapman, Kent D. ^[2]; Guo, Liang ^[4]

Huazhong Agricultural Univ., Wuhan (China). National Key Lab. of Crop Genetic Improvement; OSTI
Univ. of North Texas, Denton, TX (United States). Dept. of Biological Sciences. Center for Plant Lipid Research; Univ. of North Texas, Denton, TX (United States). BioDiscovery Inst.
Univ. of North Texas, Denton, TX (United States). Dept. of Biological Sciences. Center for Plant Lipid Research; Univ. of North Texas, Denton, TX (United States). BioDiscovery Inst.; Univ. of Texas Southwestern Medical Center, Dallas, TX (United States)
Huazhong Agricultural Univ., Wuhan (China). National Key Lab. of Crop Genetic Improvement

Brassica napus (B. napus) is the world's most widely grown temperate oilseed crop. Although breeding for human consumption has led to removal of erucic acid from refined canola oils, there is renewed interest in the industrial uses of erucic acid derived from B. napus, and there is a rich germplasm available for use. Here, low- and high-erucic acid accessions of B. napus seeds were examined for the distribution of erucic acid-containing lipids and the gene transcripts encoding the enzymes involved in pathways for its incorporation into triacylglycerols (TAGs) across the major tissues of the seeds. In general, the results indicate that a heterogeneous distribution of erucic acid across B. napus seed tissues was contributed by two isoforms (out of six) of FATTY ACYL COA ELONGASE (FAE1) and a combination of phospholipid:diacylglycerol acyltransferase (PDAT)- and diacylglycerol acyltransferase (DGAT)-mediated incorporation of erucic acid into TAGs in cotyledonary tissues. An absence of the expression of these two FAE1 isoforms accounted for the absence of erucic acid in the TAGs of the lowerucic accession.

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Research Organization:: Univ. of North Texas, Denton, TX (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0020325

OSTI ID:: 1803957

Journal Information:: Frontiers in Plant Science, Journal Name: Frontiers in Plant Science Vol. 10; ISSN 1664-462X

Publisher:: Frontiers Research FoundationCopyright Statement

Country of Publication:: United States

Language:: English

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The impact of reducing fatty acid desaturation on the composition and thermal stability of rapeseed oil Kaur, Harjeevan; Wang, Lihong; Stawniak, Natalia Plant Biotechnology Journal, Vol. 18, Issue 4 https://doi.org/10.1111/pbi.13263	journal	October 2019
Imaging heterogeneity of membrane and storage lipids in transgenic Camelina sativa seeds with altered fatty acid profiles Horn, Patrick J.; Silva, Jillian E.; Anderson, Danielle The Plant Journal https://doi.org/10.1111/tpj.12278	journal	July 2013
Quantitative profiling and pattern analysis of triacylglycerol species in Arabidopsis seeds by electrospray ionization mass spectrometry Li, Maoyin; Baughman, Ethan; Roth, Mary R. The Plant Journal, Vol. 77, Issue 1 https://doi.org/10.1111/tpj.12365	journal	November 2013
Spatial analysis of lipid metabolites and expressed genes reveals tissue-specific heterogeneity of lipid metabolism in high- and low-oil Brassica napus L. seeds Lu, Shaoping; Sturtevant, Drew; Aziz, Mina The Plant Journal, Vol. 94, Issue 6 https://doi.org/10.1111/tpj.13959	journal	June 2018
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Effect of replacing solvent-extracted canola meal with high-oil traditional canola, high-oleic acid canola, or high-erucic acid rapeseed meals on rumen fermentation, digestibility, milk production, and milk fatty acid composition in lactating dairy cows Hristov, A. N.; Domitrovich, C.; Wachter, A. Journal of Dairy Science, Vol. 94, Issue 8 https://doi.org/10.3168/jds.2011-4283	journal	August 2011

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59 BASIC BIOLOGICAL SCIENCES
Brassica napus
Plant Sciences
canola
erucic acid
matrix assisted laser desorption/ionizationmass spectrometry imaging
spatial distribution

Title: Heterogeneous Distribution of Erucic Acid in Brassica napus Seeds

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