Overexpression of phospholipid: diacylglycerol acyltransferase in Brassica napus results in changes in lipid metabolism and oil accumulation

Fenyk, Stepan; Woodfield, Helen K.; Romsdahl, Trevor B.; Wallington, Emma J.; Bates, Ruth E.; Fell, David A.; Chapman, Kent D.; Fawcett, Tony; Harwood, John L.

doi:10.1042/bcj20220003

Title: Overexpression of phospholipid: diacylglycerol acyltransferase in Brassica napus results in changes in lipid metabolism and oil accumulation

Journal Article · Thu Mar 31 00:00:00 EDT 2022 · Biochemical Journal

DOI:https://doi.org/10.1042/bcj20220003· OSTI ID:1978923

Fenyk, Stepan ^[1]; Woodfield, Helen K. ^[2]; Romsdahl, Trevor B. ^[3]; Wallington, Emma J. ^[4]; Bates, Ruth E. ^[4];

^[5];

^[3];

^[1];

^[2]

Department of Biosciences, Durham University, Durham DH1 3LE, U.K.
School of Biosciences, Cardiff University, Cardiff CF10 3AX, U.K.
Department of Biological Sciences, BioDiscovery Institute, University of North Texas, Denton, Texas 76203-5017, U.S.A
NIAB, 93 Lawrence Weaver Road, Cambridge CB3 0LE, U.K.
Department of Biological and Medical Sciences, Oxford Brookes University, Oxford OX3 0BP, U.K.

The regulation of lipid metabolism in oil seeds is still not fully understood and increasing our knowledge in this regard is of great economic, as well as intellectual, importance. Oilseed rape (Brassica napus) is a major global oil crop where increases in triacylglycerol (TAG) accumulation have been achieved by overexpression of relevant biosynthetic enzymes. In this study, we expressed Arabidopsis phospholipid: diacylglycerol acyltransferase (PDAT1), one of the two major TAG-forming plant enzymes in B. napus DH12075 to evaluate its effect on lipid metabolism in developing seeds and to estimate its flux control coefficient. Despite several-fold increase in PDAT activity, seeds of three independently generated PDAT transgenic events showed a small but consistent decrease in seed oil content and had altered fatty acid composition of phosphoglycerides and TAG, towards less unsaturation. Mass spectrometry imaging of seed sections confirmed the shift in lipid compositions and indicated that PDAT overexpression altered the distinct heterogeneous distributions of phosphatidylcholine (PC) molecular species. Similar, but less pronounced, changes in TAG molecular species distributions were observed. Our data indicate that PDAT exerts a small, negative, flux control on TAG biosynthesis and could have under-appreciated effects in fine-tuning of B. napus seed lipid composition in a tissue-specific manner. This has important implications for efforts to increase oil accumulation in similar crops.

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Research Organization:: Univ. of North Texas, Denton, TX (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

DOE Contract Number:: SC0020325

OSTI ID:: 1978923

Journal Information:: Biochemical Journal, Vol. 479, Issue 6; ISSN 0264-6021

Publisher:: Biochemical Society

Country of Publication:: United States

Language:: English

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