Search Menu
DOE PAGES title logo U.S. Department of Energy
Office of Scientific and Technical Information
Search Scholarly Publications

Title: Incorporation of Membrane Proteins Into Bicontinuous Microemulsions Through Winsor‐III System‐Based Extraction

Abstract

Abstract The membrane proteins (MP) α‐synuclein (ASYN) and bacteriorhodopsin (BR) were readily incorporated into bicontinuous microemulsions (BμEs) formed by two microemulsion systems: water/heptane/Aerosol‐OT (AOT)/CK‐2,13 and water/dodecane/sodium dodecyl sulfate (SDS)/1‐pentanol. (CK‐2,13 is an alkyl ethoxylate possessing two alkyl tail groups of carbon chain length 2 and 13 and an average degree of ethoxylation of 5.6.) MP were encapsulated in BμEs through preparation of Winsor‐III systems at optimal salinity, with the anionic surfactants AOT and SDS providing the driving force for extraction. Dissolution of ASYN in BμEs greatly increased the former's α‐helicity, similar to ASYN's behavior in the presence of biomembranes, while BμE‐ and vesicle‐encapsulated BR possessed similar secondary structure. Small‐angle neutron scattering (SANS) results clearly demonstrated the direct interaction of MP with the surfactants, resulting in a decrease of surface area per volume for surfactant monolayers due to decreased surfactant efficiency. The SANS signal for ASYN was isolated through the use of neutron contrast matching for the surfactants through partial deuteration of water and oil, one of the first reports of contrast matching for BμEs in the literature. The SANS results of the contrast‐matched sample reflected similar aggregation for ASYN in BμEs as was reported previously for vesicles and SDS solution.more » This study demonstrates the potential use of BμEs as MP host systems for conducting biochemical reactions such as the conversion of sunlight into adenosine triphosphate by BR and studying the fundamental behavior of MP, such as the role of ASYN dysfunction in Parkinson's disease, as well as for isolation and purification of MP via Winsor‐III‐based extraction.« less

Authors:
ORCiD logo [1]; ORCiD logo [2]; ORCiD logo [1];  [3]; ORCiD logo [3]; ORCiD logo [3]; ORCiD logo [3]
+ Show Author Affiliations
  1. Department of Biosystems Engineering and Soil Science University of Tennessee Knoxville TN 37996 USA
  2. Neutron Scattering Division Oak Ridge National Laboratory 1 Bethel Valley Road Oak Ridge TN 37831 USA, Pfizer, Purification Process Development Andover MA 01810 USA
  3. Neutron Scattering Division Oak Ridge National Laboratory 1 Bethel Valley Road Oak Ridge TN 37831 USA
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
1786536
Resource Type:
Publisher's Accepted Manuscript
Journal Name:
Journal of Surfactants and Detergents
Additional Journal Information:
Journal Name: Journal of Surfactants and Detergents Journal Volume: 24 Journal Issue: 4; Journal ID: ISSN 1097-3958
Publisher:
Wiley Blackwell (John Wiley & Sons)
Country of Publication:
United States
Language:
English

Citation Formats

Hayes, Douglas G., Anunciado, Divina B., Ye, Ran, Williams, Rachel N. D., O'Neill, Hugh M., Pingali, Sai Venkatesh, and Urban, Volker S. Incorporation of Membrane Proteins Into Bicontinuous Microemulsions Through Winsor‐III System‐Based Extraction. United States: N. p., 2021. Web. doi:10.1002/jsde.12500.
Copy to clipboard
Hayes, Douglas G., Anunciado, Divina B., Ye, Ran, Williams, Rachel N. D., O'Neill, Hugh M., Pingali, Sai Venkatesh, & Urban, Volker S. Incorporation of Membrane Proteins Into Bicontinuous Microemulsions Through Winsor‐III System‐Based Extraction. United States. https://doi.org/10.1002/jsde.12500
Copy to clipboard
Hayes, Douglas G., Anunciado, Divina B., Ye, Ran, Williams, Rachel N. D., O'Neill, Hugh M., Pingali, Sai Venkatesh, and Urban, Volker S. Sun . "Incorporation of Membrane Proteins Into Bicontinuous Microemulsions Through Winsor‐III System‐Based Extraction". United States. https://doi.org/10.1002/jsde.12500.
Copy to clipboard
@article{osti_1786536,
title = {Incorporation of Membrane Proteins Into Bicontinuous Microemulsions Through Winsor‐III System‐Based Extraction},
author = {Hayes, Douglas G. and Anunciado, Divina B. and Ye, Ran and Williams, Rachel N. D. and O'Neill, Hugh M. and Pingali, Sai Venkatesh and Urban, Volker S.},
abstractNote = {Abstract The membrane proteins (MP) α‐synuclein (ASYN) and bacteriorhodopsin (BR) were readily incorporated into bicontinuous microemulsions (BμEs) formed by two microemulsion systems: water/heptane/Aerosol‐OT (AOT)/CK‐2,13 and water/dodecane/sodium dodecyl sulfate (SDS)/1‐pentanol. (CK‐2,13 is an alkyl ethoxylate possessing two alkyl tail groups of carbon chain length 2 and 13 and an average degree of ethoxylation of 5.6.) MP were encapsulated in BμEs through preparation of Winsor‐III systems at optimal salinity, with the anionic surfactants AOT and SDS providing the driving force for extraction. Dissolution of ASYN in BμEs greatly increased the former's α‐helicity, similar to ASYN's behavior in the presence of biomembranes, while BμE‐ and vesicle‐encapsulated BR possessed similar secondary structure. Small‐angle neutron scattering (SANS) results clearly demonstrated the direct interaction of MP with the surfactants, resulting in a decrease of surface area per volume for surfactant monolayers due to decreased surfactant efficiency. The SANS signal for ASYN was isolated through the use of neutron contrast matching for the surfactants through partial deuteration of water and oil, one of the first reports of contrast matching for BμEs in the literature. The SANS results of the contrast‐matched sample reflected similar aggregation for ASYN in BμEs as was reported previously for vesicles and SDS solution. This study demonstrates the potential use of BμEs as MP host systems for conducting biochemical reactions such as the conversion of sunlight into adenosine triphosphate by BR and studying the fundamental behavior of MP, such as the role of ASYN dysfunction in Parkinson's disease, as well as for isolation and purification of MP via Winsor‐III‐based extraction.},
doi = {10.1002/jsde.12500},
journal = {Journal of Surfactants and Detergents},
number = 4,
volume = 24,
place = {United States},
year = {Sun Mar 14 00:00:00 EST 2021},
month = {Sun Mar 14 00:00:00 EST 2021}
}
Copy to clipboard
Journal Article:
Free Publicly Available Full Text
Accepted Manuscript (Publisher)
Publisher's Version of Record
https://doi.org/10.1002/jsde.12500
Copyright Statement
Other availability
Search WorldCat Search WorldCat to find libraries that may hold this journal
Save / Share:
Export Metadata
Save to My Library
You must Sign In or Create an Account in order to save documents to your library.

Works referenced in this record:

α-Synuclein – Regulator of Exocytosis, Endocytosis, or Both?
journal, July 2017

  • Lautenschläger, Janin; Kaminski, Clemens F.; Kaminski Schierle, Gabriele S.
  • Trends in Cell Biology, Vol. 27, Issue 7
  • DOI: 10.1016/j.tcb.2017.02.002

Biomimetic hybrid membranes: incorporation of transport proteins/peptides into polymer supports
journal, January 2019

  • Puiggalí-Jou, Anna; del Valle, Luis J.; Alemán, Carlos
  • Soft Matter, Vol. 15, Issue 13
  • DOI: 10.1039/C8SM02513D

Purification and biochemical characterization of photo-active membrane protein bacteriorhodopsin from Haloarcula marismortui, an extreme halophile from the Dead Sea
journal, October 2018

  • Alsafadi, Diya; Khalili, Fawwaz I.; Juwhari, Hassan
  • International Journal of Biological Macromolecules, Vol. 118
  • DOI: 10.1016/j.ijbiomac.2018.07.045

Synergism in binary mixtures of surfactants
journal, November 1982

Dynamic structural flexibility of α-synuclein
journal, April 2016

Effect of multiple scattering on SANS spectra from bicontinuous microemulsions
journal, January 2003

Protein extraction by Winsor-III microemulsion systems
journal, June 2011

  • Gomez del Rio, Javier A.; Hayes, Douglas G.
  • Biotechnology Progress, Vol. 27, Issue 4
  • DOI: 10.1002/btpr.611

The human transmembrane proteome
journal, May 2015

Molecular mechanism of vectorial proton translocation by bacteriorhodopsin
journal, August 2000

  • Subramaniam, Sriram; Henderson, Richard
  • Nature, Vol. 406, Issue 6796
  • DOI: 10.1038/35020614

Structure, viscoelasticity, and interfacial dynamics of a model polymeric bicontinuous microemulsion
journal, January 2016

  • Hickey, Robert J.; Gillard, Timothy M.; Irwin, Matthew T.
  • Soft Matter, Vol. 12, Issue 1
  • DOI: 10.1039/C5SM02009C

Synergism in binary mixtures of surfactants: II. Some experimental data
journal, December 1982

  • Rosen, M. J.; Hua, X. Y.
  • Journal of the American Oil Chemists' Society, Vol. 59, Issue 12
  • DOI: 10.1007/BF02636329

Removal of non-ionic organic pollutants from water via liquid–liquid extraction
journal, May 2005

Mass fractal dimension and the compactness of proteins
journal, January 2005

Activity of squalene-hopene cyclases in bicontinuous microemulsions
journal, November 2015

The critical micelle concentration of sodium dodecyl sulfate-bivalent metal dodecyl sulfate mixtures in aqueous solutions
journal, January 1974

  • Moroi, Yoshikiyo; Motomura, Kinsi; Matuura, Ryohei
  • Journal of Colloid and Interface Science, Vol. 46, Issue 1
  • DOI: 10.1016/0021-9797(74)90030-7

Current problems and future avenues in proteoliposome research
journal, August 2020

  • Amati, Andrea Marco; Graf, Simone; Deutschmann, Sabina
  • Biochemical Society Transactions, Vol. 48, Issue 4
  • DOI: 10.1042/BST20190966

Electrochemistry in bicontinuous microemulsions based on control of dynamic solution structures on electrode surfaces
journal, October 2016

  • Kunitake, Masashi; Kuraya, Eisuke; Kato, Dai
  • Current Opinion in Colloid & Interface Science, Vol. 25
  • DOI: 10.1016/j.cocis.2016.05.004

Large scale preparation of homogeneous bacteriorhodopsin
journal, February 1990

A new method for purification of recombinant human α-synuclein in Escherichia coli
journal, July 2005

  • Huang, Chunjuan; Ren, Guoping; Zhou, Hui
  • Protein Expression and Purification, Vol. 42, Issue 1
  • DOI: 10.1016/j.pep.2005.02.014

Production of membrane proteins in industry: The example of GPCRs
journal, May 2020

Synthesis of pH-Degradable Nonionic Surfactants and Their Applications in Microemulsions
journal, October 2001

  • Iyer, Maithili; Hayes, Douglas G.; Harris, J. Milton
  • Langmuir, Vol. 17, Issue 22
  • DOI: 10.1021/la010494t

Purification of a Membrane Protein with Conjugated Engineered Micelles
journal, June 2013

  • Patchornik, Guy; Danino, Dganit; Kesselman, Ellina
  • Bioconjugate Chemistry, Vol. 24, Issue 7
  • DOI: 10.1021/bc400069w

Confinement Effects in Block Copolymer Modified Bicontinuous Microemulsions
journal, April 2013

  • Maccarrone, Simona; Byelov, Dmytro V.; Auth, Thorsten
  • The Journal of Physical Chemistry B, Vol. 117, Issue 18
  • DOI: 10.1021/jp402825d

Scattering intensity of bicontinuous microemulsions and sponge phases
journal, April 2012

  • Peltomäki, Matti; Gompper, Gerhard; Kroll, Daniel M.
  • The Journal of Chemical Physics, Vol. 136, Issue 13
  • DOI: 10.1063/1.3701265

Bicontinuous microemulsions as a biomembrane mimetic system for melittin
journal, February 2018

  • Hayes, Douglas G.; Ye, Ran; Dunlap, Rachel N.
  • Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1860, Issue 2
  • DOI: 10.1016/j.bbamem.2017.11.005

Small‐angle neutron scattering from microemulsions near the disorder line in water/formamide–octane‐C i E j systems
journal, December 1991

  • Schubert, K. ‐V.; Strey, R.
  • The Journal of Chemical Physics, Vol. 95, Issue 11
  • DOI: 10.1063/1.461282

One-step purification of delipidated Bacteriorhodopsin by aqueous-three-phase system from purple membrane of Halobacterium
journal, April 2014

  • Shiu, Pei-Jiun; Chen, Hsiu-Mei; Lee, Cheng-Kang
  • Food and Bioproducts Processing, Vol. 92, Issue 2
  • DOI: 10.1016/j.fbp.2014.01.003

Small-angle neutron scattering reveals the assembly of alpha-synuclein in lipid membranes
journal, December 2015

  • Anunciado, Divina; Rai, Durgesh K.; Qian, Shuo
  • Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics, Vol. 1854, Issue 12
  • DOI: 10.1016/j.bbapap.2015.08.009

Approximations Leading to a Unified Exponential/Power-Law Approach to Small-Angle Scattering
journal, December 1995

Available Know-how in Transforming an Emulsified Drilling Fluid To Be Removed from an Unwanted Location into a Low-Viscosity Single-Phase System
journal, April 2012

  • Salager, Jean-Louis; Forgiarini, Ana María; Antón, Raquel Elena
  • Energy & Fuels, Vol. 26, Issue 7
  • DOI: 10.1021/ef300260v

Measuring bending rigidity and spatial renormalization in bicontinuous microemulsions
journal, December 2001

α-synuclein toxicity in neurodegeneration: mechanism and therapeutic strategies
journal, February 2017

  • Wong, Yvette C.; Krainc, Dimitri
  • Nature Medicine, Vol. 23, Issue 2
  • DOI: 10.1038/nm.4269

Triacylglycerol microemulsions stabilized by alkyl ethoxylate surfactants—A basic study
journal, August 2007

  • Engelskirchen, S.; Elsner, N.; Sottmann, T.
  • Journal of Colloid and Interface Science, Vol. 312, Issue 1
  • DOI: 10.1016/j.jcis.2006.09.022

Current strategies for protein production and purification enabling membrane protein structural biology
journal, December 2016

  • Pandey, Aditya; Shin, Kyungsoo; Patterson, Robin E.
  • Biochemistry and Cell Biology, Vol. 94, Issue 6
  • DOI: 10.1139/bcb-2015-0143

Experimental determination of bending rigidity and saddle splay modulus in bicontinuous microemulsions
journal, January 2013

  • Holderer, Olaf; Frielinghaus, Henrich; Monkenbusch, Michael
  • Soft Matter, Vol. 9, Issue 7
  • DOI: 10.1039/c2sm27449c

Temperature dependence of the surfactant film bending elasticity in a bicontinuous sugar surfactant based microemulsion: a quasielastic scattering study
journal, January 2011

  • Wellert, Stefan; Karg, Matthias; Holderer, Olaf
  • Phys. Chem. Chem. Phys., Vol. 13, Issue 8
  • DOI: 10.1039/C0CP02044C

Silicone Microemulsion Structures Are Maintained During Polymerization with Reactive Surfactants
journal, March 2018

Reduction and analysis of SANS and USANS data using IGOR Pro
journal, November 2006

Reconstitution of Membrane Proteins into Model Membranes: Seeking Better Ways to Retain Protein Activities
journal, January 2013

  • Shen, Hsin-Hui; Lithgow, Trevor; Martin, Lisa
  • International Journal of Molecular Sciences, Vol. 14, Issue 1
  • DOI: 10.3390/ijms14011589

Membrane protein nanoparticles: the shape of things to come
journal, November 2018

  • Simon, Kailene S.; Pollock, Naomi L.; Lee, Sarah C.
  • Biochemical Society Transactions, Vol. 46, Issue 6
  • DOI: 10.1042/BST20180139

UV-LED induced bicontinuous microemulsions polymerisation for surface modification of commercial membranes – Enhancing the antifouling properties
journal, April 2018

  • Galiano, Francesco; André Schmidt, Stefan; Ye, Xiaoyun
  • Separation and Purification Technology, Vol. 194
  • DOI: 10.1016/j.seppur.2017.10.063

Small angle neutron scattering near Lifshitz lines: Transition from weakly structured mixtures to microemulsions
journal, September 1994

  • Schubert, K. ‐V.; Strey, R.; Kline, S. R.
  • The Journal of Chemical Physics, Vol. 101, Issue 6
  • DOI: 10.1063/1.467387

Protein Data Bank: the single global archive for 3D macromolecular structure data
journal, October 2018

  • Burley, Stephen K.; Berman, Helen M.; Bhikadiya, Charmi
  • Nucleic Acids Research, Vol. 47, Issue D1
  • DOI: 10.1093/nar/gky949

The Bio-SANS instrument at the High Flux Isotope Reactor of Oak Ridge National Laboratory
journal, June 2014

  • Heller, William T.; Urban, Volker S.; Lynn, Gary W.
  • Journal of Applied Crystallography, Vol. 47, Issue 4
  • DOI: 10.1107/S1600576714011285

Segregation of Amphiphilic Polymer-Coated Nanoparticles to Bicontinuous Oil/Water Microemulsion Phases
journal, February 2017

Lecithin-Linker Microemulsion Gelatin Gels for Extended Drug Delivery
journal, January 2012

Using self-cleavable ternary fusion pattern for efficient preparation of Bacteriorhodopsin
journal, January 2018

Effect of amphiphilic block copolymers on the structure and phase behavior of oil–water-surfactant mixtures
journal, July 2001

  • Endo, H.; Mihailescu, M.; Monkenbusch, M.
  • The Journal of Chemical Physics, Vol. 115, Issue 1
  • DOI: 10.1063/1.1377881

Bio-nano hybrid materials based on bacteriorhodopsin: Potential applications and future strategies
journal, November 2015

  • Mahyad, Baharak; Janfaza, Sajjad; Hosseini, Elaheh Sadat
  • Advances in Colloid and Interface Science, Vol. 225
  • DOI: 10.1016/j.cis.2015.09.006

Biomimetic Membranes with Transmembrane Proteins: State-of-the-Art in Transmembrane Protein Applications
journal, March 2019

  • Ryu, Hyunil; Fuwad, Ahmed; Yoon, Sunhee
  • International Journal of Molecular Sciences, Vol. 20, Issue 6
  • DOI: 10.3390/ijms20061437
    Sort by:
    [ × clear filter / sort ]

    Similar Records in DOE PAGES and OSTI.GOV collections: