Incorporation of Melittin Enhances Interfacial Fluidity of Bicontinuous Microemulsions
Abstract
The effect of an antimicrobial peptide, melittin, on the long-range collective and local undulation motions of bicontinuous microemulsions (BμEs) was evaluated using dynamic light scattering (DLS) and neutron spin echo (NSE) spectroscopy. Small-angle neutron scattering (SANS) was also used to study the effect of melittin on the structure and the correlation length of BμEs. BμEs were isolated from Winsor-III systems formed by mixing aqueous melittin solutions containing sodium dodecyl sulfate with dodecane/1-pentanol mixtures at optimal salinity. An increase of melittin concentration linearly increased the diffusivity of the microemulsions (DDLS) and decreased the bending rigidity (κNSE) up to an aqueous melittin concentration of 2 g/L, thus indicating an increase of interfacial fluidity. Further increase of melittin concentration above 2 g/L did not change DDLS and slightly increased κNSE. The changes reflect differences of interaction between melittin and BμE surfactant monolayers at different melittin concentrations. At low concentrations, melittin is highly associated with the surfactant monolayers, likely ion-paired to the sulfate surfactant head groups and perhaps partially penetrating into the surfactant tail region. Above 2 g/L, an apparent saturation concentration, added melittin is less strongly associated with the monolayers. In contrast, the bare bending constant derived from SANS data (κbare) underwent onlymore »
- Authors:
-
- Bhabha Atomic Research Centre, Mumbai (India)
- Univ. of Tennessee, Knoxville, TN (United States)
- Louisiana State Univ., Baton Rouge, LA (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Spallation Neutron Source (SNS) outstation Juelich Centre for Neutron Science (JCNS), Forschungszentrum Juelich GmbH
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Neutron Sciences Directorate
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). Spallation Neutron Source (SNS) outstation Juelich Centre for Neutron Science (JCNS), Forschungszentrum Juelich GmbH
- Publication Date:
- Research Org.:
- Louisiana State Univ., Baton Rouge, LA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1615940
- Grant/Contract Number:
- SC0012432
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Journal of Physical Chemistry. C
- Additional Journal Information:
- Journal Volume: 123; Journal Issue: 17; Journal ID: ISSN 1932-7447
- Publisher:
- American Chemical Society
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 75 CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY; Lipids; Sodium dodecyl sulfate; Dynamic light scattering; Monolayers; Surfactants; antimicrobial peptides; bicontinuous microemulsions; dynamics; collective motions; elasticity; bending modulus; neutron spin echo; small-angle neutron scattering; dynamic light scattering; sodium dodecyl sulfate; winsor-III systems; melittin
Citation Formats
Sharma, V. K., Hayes, D. G., Gupta, S., Urban, V. S., O’Neill, H. M., Pingali, S. V., Ohl, M., and Mamontov, E. Incorporation of Melittin Enhances Interfacial Fluidity of Bicontinuous Microemulsions. United States: N. p., 2019.
Web. doi:10.1021/acs.jpcc.9b00103.
Sharma, V. K., Hayes, D. G., Gupta, S., Urban, V. S., O’Neill, H. M., Pingali, S. V., Ohl, M., & Mamontov, E. Incorporation of Melittin Enhances Interfacial Fluidity of Bicontinuous Microemulsions. United States. https://doi.org/10.1021/acs.jpcc.9b00103
Sharma, V. K., Hayes, D. G., Gupta, S., Urban, V. S., O’Neill, H. M., Pingali, S. V., Ohl, M., and Mamontov, E. Fri .
"Incorporation of Melittin Enhances Interfacial Fluidity of Bicontinuous Microemulsions". United States. https://doi.org/10.1021/acs.jpcc.9b00103. https://www.osti.gov/servlets/purl/1615940.
@article{osti_1615940,
title = {Incorporation of Melittin Enhances Interfacial Fluidity of Bicontinuous Microemulsions},
author = {Sharma, V. K. and Hayes, D. G. and Gupta, S. and Urban, V. S. and O’Neill, H. M. and Pingali, S. V. and Ohl, M. and Mamontov, E.},
abstractNote = {The effect of an antimicrobial peptide, melittin, on the long-range collective and local undulation motions of bicontinuous microemulsions (BμEs) was evaluated using dynamic light scattering (DLS) and neutron spin echo (NSE) spectroscopy. Small-angle neutron scattering (SANS) was also used to study the effect of melittin on the structure and the correlation length of BμEs. BμEs were isolated from Winsor-III systems formed by mixing aqueous melittin solutions containing sodium dodecyl sulfate with dodecane/1-pentanol mixtures at optimal salinity. An increase of melittin concentration linearly increased the diffusivity of the microemulsions (DDLS) and decreased the bending rigidity (κNSE) up to an aqueous melittin concentration of 2 g/L, thus indicating an increase of interfacial fluidity. Further increase of melittin concentration above 2 g/L did not change DDLS and slightly increased κNSE. The changes reflect differences of interaction between melittin and BμE surfactant monolayers at different melittin concentrations. At low concentrations, melittin is highly associated with the surfactant monolayers, likely ion-paired to the sulfate surfactant head groups and perhaps partially penetrating into the surfactant tail region. Above 2 g/L, an apparent saturation concentration, added melittin is less strongly associated with the monolayers. In contrast, the bare bending constant derived from SANS data (κbare) underwent only a minor decrease. Noting that κbare reflects contributions from both the elasticity and saddle-splay moduli, this suggests that κbare is not an effective indicator of dynamics for the BμEs investigated. Our study demonstrates that amphiphilic solubilizates can have a complex impact on dynamics of surfactant monolayers in microemulsions, with implications for transmembrane diffusion and the kinetics of release from the microemulsion phase, which are important characteristics for the use of BμEs as delivery vehicles and host systems for (bio)chemical reactions.},
doi = {10.1021/acs.jpcc.9b00103},
journal = {Journal of Physical Chemistry. C},
number = 17,
volume = 123,
place = {United States},
year = {Fri Mar 22 00:00:00 EDT 2019},
month = {Fri Mar 22 00:00:00 EDT 2019}
}
Web of Science
Works referenced in this record:
Process of inducing pores in membranes by melittin
journal, August 2013
- Lee, M. -T.; Sun, T. -L.; Hung, W. -C.
- Proceedings of the National Academy of Sciences, Vol. 110, Issue 35
Analysis of Macromolecular Polydispersity in Intensity Correlation Spectroscopy: The Method of Cumulants
journal, December 1972
- Koppel, Dennis E.
- The Journal of Chemical Physics, Vol. 57, Issue 11
Making Sense of Brownian Motion: Colloid Characterization by Dynamic Light Scattering
journal, July 2014
- Hassan, Puthusserickal A.; Rana, Suman; Verma, Gunjan
- Langmuir, Vol. 31, Issue 1
Dynamics of Phospholipid Membranes beyond Thermal Undulations
journal, May 2018
- Gupta, Sudipta; De Mel, Judith U.; Perera, Rasangi M.
- The Journal of Physical Chemistry Letters, Vol. 9, Issue 11
Shape and size fluctuations of microemulsion droplets: The role of cosurfactant
journal, December 1990
- Farago, B.; Richter, D.; Huang, J. S.
- Physical Review Letters, Vol. 65, Issue 26
Role of lipids in the interaction of antimicrobial peptides with membranes
journal, April 2012
- Teixeira, Vitor; Feio, Maria J.; Bastos, Margarida
- Progress in Lipid Research, Vol. 51, Issue 2
Bending moduli of microemulsions; comparison of results from small angle neutron scattering and neutron spin-echo spectroscopy
journal, July 2005
- Monkenbusch, M.; Holderer, O.; Frielinghaus, H.
- Journal of Physics: Condensed Matter, Vol. 17, Issue 31
Phase Behavior of Ionic Microemulsions
journal, March 1996
- Lekkerkerker, H. N. W.; Kegel, W. K.; Overbeek, J. Th. G.
- Berichte der Bunsengesellschaft für physikalische Chemie, Vol. 100, Issue 3
The role of electrostatic interactions in the membrane binding of melittin
journal, March 2010
- Hall, Kristopher; Lee, Tzong-Hsien; Aguilar, Marie-Isabel
- Journal of Molecular Recognition, Vol. 24, Issue 1
The high-resolution neutron spin-echo spectrometer for the SNS with τ⩾1μs
journal, July 2004
- Ohl, M.; Monkenbusch, M.; Richter, D.
- Physica B: Condensed Matter, Vol. 350, Issue 1-3
Contribution of the electric double layer to the curvature elasticity of charged amphiphilic monolayers
journal, September 1989
- Lekkerkerker, H. N. W.
- Physica A: Statistical Mechanics and its Applications, Vol. 159, Issue 3
Antimicrobial Peptide Action on Parasites
journal, July 2012
- Torrent, Marc; Pulido, David; Rivas, Luis
- Current Drug Targets, Vol. 13, Issue 9
Protein extraction by Winsor-III microemulsion systems
journal, June 2011
- Gomez del Rio, Javier A.; Hayes, Douglas G.
- Biotechnology Progress, Vol. 27, Issue 4
Observation of a Topological Relaxation Mode in Microemulsions
journal, April 2001
- Peter, U.; Roux, D.; Sood, A. K.
- Physical Review Letters, Vol. 86, Issue 15
Amphiphilic block copolymers in oil-water-surfactant mixtures: efficiency boosting, structure, phase behaviour and mechanism
journal, September 2001
- Gompper, G.; Richter, D.; Strey, R.
- Journal of Physics: Condensed Matter, Vol. 13, Issue 41
Viscosity of deuterium oxide and water in the range 5 to 125 C
journal, June 1949
- Hardy, Robert C.; Cottington, Robert L.
- Journal of Research of the National Bureau of Standards, Vol. 42, Issue 6
Nanoscopic dynamics of bicontinous microemulsions: effect of membrane associated protein
journal, January 2017
- Sharma, V. K.; Hayes, Douglas G.; Urban, Volker S.
- Soft Matter, Vol. 13, Issue 28
Removal of non-ionic organic pollutants from water via liquid–liquid extraction
journal, May 2005
- López-Montilla, Juan C.; Pandey, Samir; Shah, Dinesh O.
- Water Research, Vol. 39, Issue 9
APD3: the antimicrobial peptide database as a tool for research and education
journal, November 2015
- Wang, Guangshun; Li, Xia; Wang, Zhe
- Nucleic Acids Research, Vol. 44, Issue D1
Effect of Protein Incorporation on the Nanostructure of the Bicontinuous Microemulsion Phase of Winsor-III Systems: A Small-Angle Neutron Scattering Study
journal, February 2015
- Hayes, Douglas G.; Gomez del Rio, Javier A.; Ye, Ran
- Langmuir, Vol. 31, Issue 6
Dynamic Light Scattering Study of Concentrated W/O Microemulsions
journal, December 1980
- Bellocq, A. M.; Fourche, G.; Chabrat, P.
- Optica Acta: International Journal of Optics, Vol. 27, Issue 12
Internal Dynamics in SDS Micelles: Neutron Scattering Study
journal, December 2010
- Sharma, V. K.; Mitra, S.; Verma, G.
- The Journal of Physical Chemistry B, Vol. 114, Issue 51
Dynamics of bicontinuous microemulsion phases with and without amphiphilic block-copolymers
journal, November 2001
- Mihailescu, M.; Monkenbusch, M.; Endo, H.
- The Journal of Chemical Physics, Vol. 115, Issue 20
Interlayer distance dependence of thickness fluctuations in a swollen lamellar phase
journal, January 2011
- Nagao, Michihiro; Chawang, Sukhum; Hawa, Takumi
- Soft Matter, Vol. 7, Issue 14
The membrane interactions of antimicrobial peptides revealed by solid-state NMR spectroscopy
journal, April 2012
- Bechinger, Burkhard; Salnikov, Evgeniy S.
- Chemistry and Physics of Lipids, Vol. 165, Issue 3
Dynamical and Phase Behavior of a Phospholipid Membrane Altered by an Antimicrobial Peptide at Low Concentration
journal, June 2016
- Sharma, V. K.; Mamontov, E.; Tyagi, M.
- The Journal of Physical Chemistry Letters, Vol. 7, Issue 13
Confinement Effects in Block Copolymer Modified Bicontinuous Microemulsions
journal, April 2013
- Maccarrone, Simona; Byelov, Dmytro V.; Auth, Thorsten
- The Journal of Physical Chemistry B, Vol. 117, Issue 18
Dynamics of ternary polymer blends: Disordered, ordered and bicontinuous microemulsion phases
journal, January 1999
- Morkved, Terry L.; Chapman, Bryan R.; Bates, Frank S.
- Faraday Discussions, Vol. 112
Scattering intensity of bicontinuous microemulsions and sponge phases
journal, April 2012
- Peltomäki, Matti; Gompper, Gerhard; Kroll, Daniel M.
- The Journal of Chemical Physics, Vol. 136, Issue 13
Melittin peptide kills Trypanosoma cruzi parasites by inducing different cell death pathways
journal, July 2013
- Adade, Camila M.; Oliveira, Isabelle R. S.; Pais, Joana A. R.
- Toxicon, Vol. 69
Bicontinuous microemulsions as a biomembrane mimetic system for melittin
journal, February 2018
- Hayes, Douglas G.; Ye, Ran; Dunlap, Rachel N.
- Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1860, Issue 2
Detergent-like actions of linear amphipathic cationic antimicrobial peptides
journal, September 2006
- Bechinger, Burkhard; Lohner, Karl
- Biochimica et Biophysica Acta (BBA) - Biomembranes, Vol. 1758, Issue 9
Small‐angle neutron scattering from microemulsions near the disorder line in water/formamide–octane‐C i E j systems
journal, December 1991
- Schubert, K. ‐V.; Strey, R.
- The Journal of Chemical Physics, Vol. 95, Issue 11
The Bio-SANS instrument at the High Flux Isotope Reactor of Oak Ridge National Laboratory
journal, June 2014
- Heller, William T.; Urban, Volker S.; Lynn, Gary W.
- Journal of Applied Crystallography, Vol. 47, Issue 4
Measuring bending rigidity and spatial renormalization in bicontinuous microemulsions
journal, December 2001
- Gompper, G.; Endo, H.; Mihailescu, M.
- Europhysics Letters (EPL), Vol. 56, Issue 5
Triacylglycerol microemulsions stabilized by alkyl ethoxylate surfactants—A basic study
journal, August 2007
- Engelskirchen, S.; Elsner, N.; Sottmann, T.
- Journal of Colloid and Interface Science, Vol. 312, Issue 1
Experimental determination of bending rigidity and saddle splay modulus in bicontinuous microemulsions
journal, January 2013
- Holderer, Olaf; Frielinghaus, Henrich; Monkenbusch, Michael
- Soft Matter, Vol. 9, Issue 7
Correlation of high-pressure diffusion and viscosity coefficients for n-alkanes
journal, September 1989
- Dymond, J. H.; Awan, M. A.
- International Journal of Thermophysics, Vol. 10, Issue 5
Silicone Microemulsion Structures Are Maintained During Polymerization with Reactive Surfactants
journal, March 2018
- Whinton, Marlena; Hughes, Timothy C.; Peng, Shuhua
- Langmuir, Vol. 34, Issue 14
Protein extraction into the bicontinuous microemulsion phase of a Water/SDS/pentanol/dodecane winsor-III system: Effect on nanostructure and protein conformation
journal, December 2017
- Hayes, Douglas G.; Ye, Ran; Dunlap, Rachel N.
- Colloids and Surfaces B: Biointerfaces, Vol. 160
Antimicrobial peptides: pore formers or metabolic inhibitors in bacteria?
journal, March 2005
- Brogden, Kim A.
- Nature Reviews Microbiology, Vol. 3, Issue 3
The Anti-Arthritic Effects of Synthetic Melittin on the Complete Freund's Adjuvant-Induced Rheumatoid Arthritis Model in Rats
journal, January 2010
- Li, Jinghua; Ke, Tao; He, Chao
- The American Journal of Chinese Medicine, Vol. 38, Issue 06
UV-LED induced bicontinuous microemulsions polymerisation for surface modification of commercial membranes – Enhancing the antifouling properties
journal, April 2018
- Galiano, Francesco; André Schmidt, Stefan; Ye, Xiaoyun
- Separation and Purification Technology, Vol. 194
The spin-echo spectrometer at the Spallation Neutron Source (SNS)
journal, December 2012
- Ohl, M.; Monkenbusch, M.; Arend, N.
- Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment, Vol. 696
Small angle neutron scattering near Lifshitz lines: Transition from weakly structured mixtures to microemulsions
journal, September 1994
- Schubert, K. ‐V.; Strey, R.; Kline, S. R.
- The Journal of Chemical Physics, Vol. 101, Issue 6
Segregation of Amphiphilic Polymer-Coated Nanoparticles to Bicontinuous Oil/Water Microemulsion Phases
journal, February 2017
- Qi, Luqing; ShamsiJazeyi, Hadi; Ruan, Gedeng
- Energy & Fuels, Vol. 31, Issue 2
Dynamic properties of microemulsions modified with homopolymers and diblock copolymers: The determination of bending moduli and renormalization effects
journal, March 2005
- Holderer, Olaf; Frielinghaus, Henrich; Byelov, Dmytro
- The Journal of Chemical Physics, Vol. 122, Issue 9
Melittin restores proteasome function in an animal model of ALS
journal, January 2011
- Yang, Eun Jin; Kim, Seon Hwy; Yang, Sun Choel
- Journal of Neuroinflammation, Vol. 8, Issue 1
The structure of melittin. II. Interpretation of the structure.
journal, June 1982
- Terwilliger, T. C.; Eisenberg, D.
- Journal of Biological Chemistry, Vol. 257, Issue 11