Systematic discovery of pseudomonad genetic factors involved in sensitivity to tailocins
Abstract
Tailocins are bactericidal protein complexes produced by a wide variety of bacteria that kill closely related strains and may play a role in microbial community structure. Thanks to their high specificity, tailocins have been proposed as precision antibacterial agents for therapeutic applications. Compared to tailed phages, with whom they share an evolutionary and morphological relationship, bacterially produced tailocins kill their host upon production but producing strains display resistance to self-intoxication. Though lipopolysaccharide (LPS) has been shown to act as a receptor for tailocins, the breadth of factors involved in tailocin sensitivity, and the mechanisms behind resistance to self-intoxication, remain unclear. Here, we employed genome-wide screens in four non-model pseudomonads to identify mutants with altered fitness in the presence of tailocins produced by closely related pseudomonads. Our mutant screens identified O-antigen composition and display as most important in defining sensitivity to our tailocins. In addition, the screens suggest LPS thinning as a mechanism by which resistant strains can become more sensitive to tailocins. We validate many of these novel findings, and extend these observations of tailocin sensitivity to 130 genome-sequenced pseudomonads. This work offers insights into tailocin-bacteria interactions, informing the potential use of tailocins in microbiome manipulation and antibacterial therapy.
- Authors:
-
- Univ. of California, Berkeley, CA (United States)
- Univ. of California, Berkeley, CA (United States). Innovative Genomics Inst.
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- Univ. of California, Berkeley, CA (United States). Innovative Genomics Inst.; Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Biological and Environmental Research (BER); National Institutes of Health (NIH); National Science Foundation (NSF); National Center for Advancing Translational Sciences
- OSTI Identifier:
- 1773739
- Alternate Identifier(s):
- OSTI ID: 1777972
- Grant/Contract Number:
- AC02-05CH11231; S10RR025622; S10RR029668; S10RR027303; OD018174; DGE 1752814
- Resource Type:
- Accepted Manuscript
- Journal Name:
- The ISME Journal
- Additional Journal Information:
- Journal Volume: 15; Journal ID: ISSN 1751-7362
- Publisher:
- Nature Publishing Group
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Bacterial genetics; functional genomics; microbial ecology; soil microbiology
Citation Formats
Carim, Sean, Azadeh, Ashley L., Kazakov, Alexey E., Price, Morgan N., Walian, Peter J., Lui, Lauren M., Nielsen, Torben N., Chakraborty, Romy, Deutschbauer, Adam M., Mutalik, Vivek K., and Arkin, Adam P. Systematic discovery of pseudomonad genetic factors involved in sensitivity to tailocins. United States: N. p., 2021.
Web. doi:10.1038/s41396-021-00921-1.
Carim, Sean, Azadeh, Ashley L., Kazakov, Alexey E., Price, Morgan N., Walian, Peter J., Lui, Lauren M., Nielsen, Torben N., Chakraborty, Romy, Deutschbauer, Adam M., Mutalik, Vivek K., & Arkin, Adam P. Systematic discovery of pseudomonad genetic factors involved in sensitivity to tailocins. United States. https://doi.org/10.1038/s41396-021-00921-1
Carim, Sean, Azadeh, Ashley L., Kazakov, Alexey E., Price, Morgan N., Walian, Peter J., Lui, Lauren M., Nielsen, Torben N., Chakraborty, Romy, Deutschbauer, Adam M., Mutalik, Vivek K., and Arkin, Adam P. Mon .
"Systematic discovery of pseudomonad genetic factors involved in sensitivity to tailocins". United States. https://doi.org/10.1038/s41396-021-00921-1. https://www.osti.gov/servlets/purl/1773739.
@article{osti_1773739,
title = {Systematic discovery of pseudomonad genetic factors involved in sensitivity to tailocins},
author = {Carim, Sean and Azadeh, Ashley L. and Kazakov, Alexey E. and Price, Morgan N. and Walian, Peter J. and Lui, Lauren M. and Nielsen, Torben N. and Chakraborty, Romy and Deutschbauer, Adam M. and Mutalik, Vivek K. and Arkin, Adam P.},
abstractNote = {Tailocins are bactericidal protein complexes produced by a wide variety of bacteria that kill closely related strains and may play a role in microbial community structure. Thanks to their high specificity, tailocins have been proposed as precision antibacterial agents for therapeutic applications. Compared to tailed phages, with whom they share an evolutionary and morphological relationship, bacterially produced tailocins kill their host upon production but producing strains display resistance to self-intoxication. Though lipopolysaccharide (LPS) has been shown to act as a receptor for tailocins, the breadth of factors involved in tailocin sensitivity, and the mechanisms behind resistance to self-intoxication, remain unclear. Here, we employed genome-wide screens in four non-model pseudomonads to identify mutants with altered fitness in the presence of tailocins produced by closely related pseudomonads. Our mutant screens identified O-antigen composition and display as most important in defining sensitivity to our tailocins. In addition, the screens suggest LPS thinning as a mechanism by which resistant strains can become more sensitive to tailocins. We validate many of these novel findings, and extend these observations of tailocin sensitivity to 130 genome-sequenced pseudomonads. This work offers insights into tailocin-bacteria interactions, informing the potential use of tailocins in microbiome manipulation and antibacterial therapy.},
doi = {10.1038/s41396-021-00921-1},
journal = {The ISME Journal},
number = ,
volume = 15,
place = {United States},
year = {Mon Mar 01 00:00:00 EST 2021},
month = {Mon Mar 01 00:00:00 EST 2021}
}
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Dramatic evolution of body length due to postembryonic changes in cell size in a newly discovered close relative of Caenorhabditis elegans: BODY SIZE EVOLUTION IN CAENORHABDITIS
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Reconstructing the genetic history of late Neanderthals
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Biodiversity of Magnetotactic Bacteria in the Freshwater Lake Beloe Bordukovskoe, Russia
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Interlaboratory Comparison of Six Real-Time PCR Assays for Detection of Bovine Leukemia Virus Proviral DNA
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The complete chloroplast genome of Rosa berberifolia
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A high-throughput sequencing assay to comprehensively detect and characterize unicellular eukaryotes and helminths from biological and environmental samples
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Complete chloroplast genome of Exacum affine (Gentianaceae): the first plastome of the tribe Exaceae in the family Gentianaceae
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The complete chloroplast genome and phylogenetic analysis of Begonia pulchrifolia , a near endangered Begoniaceae plant
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