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Title: Fermentation of sweet sorghum derived sugars to butyric acid at high titer and productivity by a moderate thermophile Clostridium thermobutyricum at 50 °C

Abstract

In this study, a moderate thermophile Clostridium thermobutyricum is shown to ferment the sugars in sweet sorghum juice treated with invertase and supplemented with tryptone (10 g L–1) and yeast extract (10 g L–1) at 50 °C to 44 g L–1 butyrate at a calculated highest volumetric productivity of 1.45 g L–1 h–1 (molar butyrate yield of 0.85 based on sugars fermented). This volumetric productivity is among the highest reported for batch fermentations. Sugars from acid and enzyme-treated sweet sorghum bagasse were also fermented to butyrate by this organism with a molar yield of 0.81 (based on the amount of cellulose and hemicellulose). By combining the results from juice and bagasse, the calculated yield of butyric acid is approximately 90 kg per tonne of fresh sweet sorghum stalk. Here, this study demonstrates that C. thermobutyricum can be an effective microbial biocatalyst for production of bio-based butyrate from renewable feedstocks at 50 °C.

Authors:
 [1];  [1];  [1];  [1];  [1];  [1];  [1]
  1. Univ. of Florida, Gainesville, FL (United States)
Publication Date:
Research Org.:
Univ. of Florida, Gainesville, FL (United States)
Sponsoring Org.:
USDOE Office of International Affairs (IA)
OSTI Identifier:
1766805
Alternate Identifier(s):
OSTI ID: 1250354; OSTI ID: 1771308
Grant/Contract Number:  
PI0000031
Resource Type:
Accepted Manuscript
Journal Name:
Bioresource Technology
Additional Journal Information:
Journal Volume: 198; Journal Issue: 198; Journal ID: ISSN 0960-8524
Publisher:
Elsevier
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Butyrate; Fermentation; Clostridium thermobutyricum; Sweet sorghum juice; Sweet sorghum bagasse; Butyrate Fermentation Clostridium thermobutyricum Sweet sorghum juice Sweet sorghum bagasse

Citation Formats

Wang, Liang, Ou, Mark S., Nieves, Ismael, Erickson, John E., Vermerris, Wilfred, Ingram, L. O., and Shanmugam, K. T.. Fermentation of sweet sorghum derived sugars to butyric acid at high titer and productivity by a moderate thermophile Clostridium thermobutyricum at 50 °C. United States: N. p., 2015. Web. https://doi.org/10.1016/j.biortech.2015.09.062.
Wang, Liang, Ou, Mark S., Nieves, Ismael, Erickson, John E., Vermerris, Wilfred, Ingram, L. O., & Shanmugam, K. T.. Fermentation of sweet sorghum derived sugars to butyric acid at high titer and productivity by a moderate thermophile Clostridium thermobutyricum at 50 °C. United States. https://doi.org/10.1016/j.biortech.2015.09.062
Wang, Liang, Ou, Mark S., Nieves, Ismael, Erickson, John E., Vermerris, Wilfred, Ingram, L. O., and Shanmugam, K. T.. Sat . "Fermentation of sweet sorghum derived sugars to butyric acid at high titer and productivity by a moderate thermophile Clostridium thermobutyricum at 50 °C". United States. https://doi.org/10.1016/j.biortech.2015.09.062. https://www.osti.gov/servlets/purl/1766805.
@article{osti_1766805,
title = {Fermentation of sweet sorghum derived sugars to butyric acid at high titer and productivity by a moderate thermophile Clostridium thermobutyricum at 50 °C},
author = {Wang, Liang and Ou, Mark S. and Nieves, Ismael and Erickson, John E. and Vermerris, Wilfred and Ingram, L. O. and Shanmugam, K. T.},
abstractNote = {In this study, a moderate thermophile Clostridium thermobutyricum is shown to ferment the sugars in sweet sorghum juice treated with invertase and supplemented with tryptone (10 g L–1) and yeast extract (10 g L–1) at 50 °C to 44 g L–1 butyrate at a calculated highest volumetric productivity of 1.45 g L–1 h–1 (molar butyrate yield of 0.85 based on sugars fermented). This volumetric productivity is among the highest reported for batch fermentations. Sugars from acid and enzyme-treated sweet sorghum bagasse were also fermented to butyrate by this organism with a molar yield of 0.81 (based on the amount of cellulose and hemicellulose). By combining the results from juice and bagasse, the calculated yield of butyric acid is approximately 90 kg per tonne of fresh sweet sorghum stalk. Here, this study demonstrates that C. thermobutyricum can be an effective microbial biocatalyst for production of bio-based butyrate from renewable feedstocks at 50 °C.},
doi = {10.1016/j.biortech.2015.09.062},
journal = {Bioresource Technology},
number = 198,
volume = 198,
place = {United States},
year = {2015},
month = {9}
}

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Cited by: 6 works
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    Works referencing / citing this record:

    H-B-E (hexanol-butanol-ethanol) fermentation for the production of higher alcohols from syngas/waste gas
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    Techno-Economic Evaluation of Cellulosic Ethanol Production Based on Pilot Biorefinery Data: a Case Study of Sweet Sorghum Bagasse Processed via L+SScF
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