Sweet Sorghum Juice and Bagasse as Feedstocks for the Production of Optically Pure Lactic Acid by Native and Engineered Bacillus coagulans Strains
Abstract
Sweet sorghum is a bioenergy crop that produces large amounts of soluble sugars in its stems (3-7 Mg. ha-1) and generates significant amounts of bagasse (15-20 Mg .ha-1) as a lignocellulosic feedstock. These sugars can be fermented to biofuels, but also to bio-based chemicals. The market potential of the latter may be higher given the current prices of petroleum and natural gas. The yield and rate of production of optically pure D-(-)- and L-(+)-lactic acid as precursors for the bio-degradable plastic polylactide was optimized for two thermotolerant Bacillus coagulans strains. Strain 36D1 fermented the sugars in unsterilized sweet sorghum juice -1 -1 -1 at 50°C to L-(+)-lactic acid (~150 g .L ; productivity, 7.2 g .L .h ). B. coagulans strain QZ19- 2 was used to ferment sorghum juice to D-(-)-lactic acid (~125 g .L Carbohydrates in the sorghum bagasse were also fermented after pretreatment with 0.5% phosphoric acid at 190°C for 5 min. Simultaneous saccharification and co-fermentation of all the sugars (SScF) by B. coagulans resulted in a conversion of 80% of available carbohydrates to optically pure lactic acid depending on the B. coagulans strain used as the microbial biocatalyst. Liquefaction of pre-treated bagasse with cellulases before SScF (L+SScF)more »
- Authors:
-
- Univ. of Florida, Gainesville, FL (United States)
- Univ. of Florida, Gainesville, FL (United States). UF Genetics Inst.
- Publication Date:
- Research Org.:
- Univ. of Florida, Gainesville, FL (United States)
- Sponsoring Org.:
- USDOE Office of Energy Efficiency and Renewable Energy (EERE), Transportation Office. Bioenergy Technologies Office; USDA; USDOE Office of International Affairs (IA)
- OSTI Identifier:
- 1661477
- Alternate Identifier(s):
- OSTI ID: 1766558
- Grant/Contract Number:
- PI0000031; 2012-67009-19596; 2011- 10006-30358
- Resource Type:
- Accepted Manuscript
- Journal Name:
- BioEnergy Research
- Additional Journal Information:
- Journal Volume: 9; Journal Issue: 1; Journal ID: ISSN 1939-1234
- Publisher:
- Springer
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; Bacillus coagulans; Fermentation; Lactic acid; Sorghumbicolor; Bacillus coagulans - fermentation - lactic acid –Sorghum bicolor
Citation Formats
Ou, Mark S., Awasthi, Deepika, Nieves, Ismael, Wang, Liang, Erickson, John, Vermerris, Wilfred, Ingram, L. O., and Shanmugam, K. T. Sweet Sorghum Juice and Bagasse as Feedstocks for the Production of Optically Pure Lactic Acid by Native and Engineered Bacillus coagulans Strains. United States: N. p., 2015.
Web. doi:10.1007/s12155-015-9670-6.
Ou, Mark S., Awasthi, Deepika, Nieves, Ismael, Wang, Liang, Erickson, John, Vermerris, Wilfred, Ingram, L. O., & Shanmugam, K. T. Sweet Sorghum Juice and Bagasse as Feedstocks for the Production of Optically Pure Lactic Acid by Native and Engineered Bacillus coagulans Strains. United States. https://doi.org/10.1007/s12155-015-9670-6
Ou, Mark S., Awasthi, Deepika, Nieves, Ismael, Wang, Liang, Erickson, John, Vermerris, Wilfred, Ingram, L. O., and Shanmugam, K. T. Sun .
"Sweet Sorghum Juice and Bagasse as Feedstocks for the Production of Optically Pure Lactic Acid by Native and Engineered Bacillus coagulans Strains". United States. https://doi.org/10.1007/s12155-015-9670-6. https://www.osti.gov/servlets/purl/1661477.
@article{osti_1661477,
title = {Sweet Sorghum Juice and Bagasse as Feedstocks for the Production of Optically Pure Lactic Acid by Native and Engineered Bacillus coagulans Strains},
author = {Ou, Mark S. and Awasthi, Deepika and Nieves, Ismael and Wang, Liang and Erickson, John and Vermerris, Wilfred and Ingram, L. O. and Shanmugam, K. T.},
abstractNote = {Sweet sorghum is a bioenergy crop that produces large amounts of soluble sugars in its stems (3-7 Mg. ha-1) and generates significant amounts of bagasse (15-20 Mg .ha-1) as a lignocellulosic feedstock. These sugars can be fermented to biofuels, but also to bio-based chemicals. The market potential of the latter may be higher given the current prices of petroleum and natural gas. The yield and rate of production of optically pure D-(-)- and L-(+)-lactic acid as precursors for the bio-degradable plastic polylactide was optimized for two thermotolerant Bacillus coagulans strains. Strain 36D1 fermented the sugars in unsterilized sweet sorghum juice -1 -1 -1 at 50°C to L-(+)-lactic acid (~150 g .L ; productivity, 7.2 g .L .h ). B. coagulans strain QZ19- 2 was used to ferment sorghum juice to D-(-)-lactic acid (~125 g .L Carbohydrates in the sorghum bagasse were also fermented after pretreatment with 0.5% phosphoric acid at 190°C for 5 min. Simultaneous saccharification and co-fermentation of all the sugars (SScF) by B. coagulans resulted in a conversion of 80% of available carbohydrates to optically pure lactic acid depending on the B. coagulans strain used as the microbial biocatalyst. Liquefaction of pre-treated bagasse with cellulases before SScF (L+SScF) increased the productivity of lactic acid. These results show that B. coagulans is an effective biocatalyst for fermentation of all the sugars present in sweet sorghum juice and bagasse to optically pure lactic acid at high titer and productivity as feedstock for bio-based plastics.},
doi = {10.1007/s12155-015-9670-6},
journal = {BioEnergy Research},
number = 1,
volume = 9,
place = {United States},
year = {Sun Aug 30 00:00:00 EDT 2015},
month = {Sun Aug 30 00:00:00 EDT 2015}
}
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