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Title: Sweet Sorghum Juice and Bagasse as Feedstocks for the Production of Optically Pure Lactic Acid by Native and Engineered Bacillus coagulans Strains

Abstract

Sweet sorghum is a bioenergy crop that produces large amounts of soluble sugars in its stems (3-7 Mg. ha-1) and generates significant amounts of bagasse (15-20 Mg .ha-1) as a lignocellulosic feedstock. These sugars can be fermented to biofuels, but also to bio-based chemicals. The market potential of the latter may be higher given the current prices of petroleum and natural gas. The yield and rate of production of optically pure D-(-)- and L-(+)-lactic acid as precursors for the bio-degradable plastic polylactide was optimized for two thermotolerant Bacillus coagulans strains. Strain 36D1 fermented the sugars in unsterilized sweet sorghum juice -1 -1 -1 at 50°C to L-(+)-lactic acid (~150 g .L ; productivity, 7.2 g .L .h ). B. coagulans strain QZ19- 2 was used to ferment sorghum juice to D-(-)-lactic acid (~125 g .L Carbohydrates in the sorghum bagasse were also fermented after pretreatment with 0.5% phosphoric acid at 190°C for 5 min. Simultaneous saccharification and co-fermentation of all the sugars (SScF) by B. coagulans resulted in a conversion of 80% of available carbohydrates to optically pure lactic acid depending on the B. coagulans strain used as the microbial biocatalyst. Liquefaction of pre-treated bagasse with cellulases before SScF (L+SScF)more » increased the productivity of lactic acid. These results show that B. coagulans is an effective biocatalyst for fermentation of all the sugars present in sweet sorghum juice and bagasse to optically pure lactic acid at high titer and productivity as feedstock for bio-based plastics.« less

Authors:
 [1];  [1];  [1];  [1];  [1];  [2];  [1];  [1]
  1. Univ. of Florida, Gainesville, FL (United States)
  2. Univ. of Florida, Gainesville, FL (United States). UF Genetics Inst.
Publication Date:
Research Org.:
Univ. of Florida, Gainesville, FL (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE), Transportation Office. Bioenergy Technologies Office; USDA; USDOE Office of International Affairs (IA)
OSTI Identifier:
1661477
Alternate Identifier(s):
OSTI ID: 1766558
Grant/Contract Number:  
PI0000031; 2012-67009-19596; 2011- 10006-30358
Resource Type:
Accepted Manuscript
Journal Name:
BioEnergy Research
Additional Journal Information:
Journal Volume: 9; Journal Issue: 1; Journal ID: ISSN 1939-1234
Publisher:
Springer
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; Bacillus coagulans; Fermentation; Lactic acid; Sorghumbicolor; Bacillus coagulans - fermentation - lactic acid –Sorghum bicolor

Citation Formats

Ou, Mark S., Awasthi, Deepika, Nieves, Ismael, Wang, Liang, Erickson, John, Vermerris, Wilfred, Ingram, L. O., and Shanmugam, K. T. Sweet Sorghum Juice and Bagasse as Feedstocks for the Production of Optically Pure Lactic Acid by Native and Engineered Bacillus coagulans Strains. United States: N. p., 2015. Web. doi:10.1007/s12155-015-9670-6.
Ou, Mark S., Awasthi, Deepika, Nieves, Ismael, Wang, Liang, Erickson, John, Vermerris, Wilfred, Ingram, L. O., & Shanmugam, K. T. Sweet Sorghum Juice and Bagasse as Feedstocks for the Production of Optically Pure Lactic Acid by Native and Engineered Bacillus coagulans Strains. United States. https://doi.org/10.1007/s12155-015-9670-6
Ou, Mark S., Awasthi, Deepika, Nieves, Ismael, Wang, Liang, Erickson, John, Vermerris, Wilfred, Ingram, L. O., and Shanmugam, K. T. Sun . "Sweet Sorghum Juice and Bagasse as Feedstocks for the Production of Optically Pure Lactic Acid by Native and Engineered Bacillus coagulans Strains". United States. https://doi.org/10.1007/s12155-015-9670-6. https://www.osti.gov/servlets/purl/1661477.
@article{osti_1661477,
title = {Sweet Sorghum Juice and Bagasse as Feedstocks for the Production of Optically Pure Lactic Acid by Native and Engineered Bacillus coagulans Strains},
author = {Ou, Mark S. and Awasthi, Deepika and Nieves, Ismael and Wang, Liang and Erickson, John and Vermerris, Wilfred and Ingram, L. O. and Shanmugam, K. T.},
abstractNote = {Sweet sorghum is a bioenergy crop that produces large amounts of soluble sugars in its stems (3-7 Mg. ha-1) and generates significant amounts of bagasse (15-20 Mg .ha-1) as a lignocellulosic feedstock. These sugars can be fermented to biofuels, but also to bio-based chemicals. The market potential of the latter may be higher given the current prices of petroleum and natural gas. The yield and rate of production of optically pure D-(-)- and L-(+)-lactic acid as precursors for the bio-degradable plastic polylactide was optimized for two thermotolerant Bacillus coagulans strains. Strain 36D1 fermented the sugars in unsterilized sweet sorghum juice -1 -1 -1 at 50°C to L-(+)-lactic acid (~150 g .L ; productivity, 7.2 g .L .h ). B. coagulans strain QZ19- 2 was used to ferment sorghum juice to D-(-)-lactic acid (~125 g .L Carbohydrates in the sorghum bagasse were also fermented after pretreatment with 0.5% phosphoric acid at 190°C for 5 min. Simultaneous saccharification and co-fermentation of all the sugars (SScF) by B. coagulans resulted in a conversion of 80% of available carbohydrates to optically pure lactic acid depending on the B. coagulans strain used as the microbial biocatalyst. Liquefaction of pre-treated bagasse with cellulases before SScF (L+SScF) increased the productivity of lactic acid. These results show that B. coagulans is an effective biocatalyst for fermentation of all the sugars present in sweet sorghum juice and bagasse to optically pure lactic acid at high titer and productivity as feedstock for bio-based plastics.},
doi = {10.1007/s12155-015-9670-6},
journal = {BioEnergy Research},
number = 1,
volume = 9,
place = {United States},
year = {Sun Aug 30 00:00:00 EDT 2015},
month = {Sun Aug 30 00:00:00 EDT 2015}
}

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Cited by: 24 works
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Works referenced in this record:

Optimizing cellulase usage for improved mixing and rheological properties of acid-pretreated sugarcane bagasse
journal, December 2010


Control of the shift from homolactic acid to mixed-acid fermentation in Lactococcus lactis: predominant role of the NADH/NAD+ ratio.
journal, January 1997


Fuel Versus Food
journal, October 2009


Production of L(+) lactic acid from sweet sorghum, date palm, and golden syrup as alternative carbon sources
journal, June 2011

  • Yadav, Anil K.; Bipinraj, Nirichan K.; Chaudhari, Ambalal B.
  • Starch - Stärke, Vol. 63, Issue 10
  • DOI: 10.1002/star.201100006

Flux through Citrate Synthase Limits the Growth of Ethanologenic Escherichia coli KO11 during Xylose Fermentation
journal, March 2002


Planting Date Affects Biomass and Brix of Sweet Sorghum Grown for Biofuel across Florida
journal, November 2011


Food vs. Fuel: Diversion of Crops Could Cause More Hunger
journal, June 2008

  • Tenenbaum, David J.
  • Environmental Health Perspectives, Vol. 116, Issue 6
  • DOI: 10.1289/ehp.116-a254

Engineering furfural tolerance in Escherichia coli improves the fermentation of lignocellulosic sugars into renewable chemicals
journal, February 2013

  • Wang, X.; Yomano, L. P.; Lee, J. Y.
  • Proceedings of the National Academy of Sciences, Vol. 110, Issue 10, p. 4021-4026
  • DOI: 10.1073/pnas.1217958110

Composition of sugar cane, energy cane, and sweet sorghum suitable for ethanol production at Louisiana sugar mills
journal, August 2010

  • Kim, Misook; Day, Donal F.
  • Journal of Industrial Microbiology & Biotechnology, Vol. 38, Issue 7
  • DOI: 10.1007/s10295-010-0812-8

Pyruvate Formate Lyase and Acetate Kinase Are Essential for Anaerobic Growth of Escherichia coli on Xylose
journal, November 2004


Evolution of D-lactate dehydrogenase activity from glycerol dehydrogenase and its utility for D-lactate production from lignocellulose
journal, November 2011

  • Wang, Q.; Ingram, L. O.; Shanmugam, K. T.
  • Proceedings of the National Academy of Sciences, Vol. 108, Issue 47, p. 18920-18925
  • DOI: 10.1073/pnas.1111085108

Silencing of NADPH-Dependent Oxidoreductase Genes (yqhD and dkgA) in Furfural-Resistant Ethanologenic Escherichia coli
journal, May 2009

  • Miller, E. N.; Jarboe, L. R.; Yomano, L. P.
  • Applied and Environmental Microbiology, Vol. 75, Issue 13, p. 4315-4323
  • DOI: 10.1128/AEM.00567-09

Forage and biomass feedstock production from hybrid forage sorghum and sorghum-sudangrass hybrids
journal, December 2008


Simplified process for ethanol production from sugarcane bagasse using hydrolysate-resistant Escherichia coli strain MM160
journal, February 2011


Recent advances in lactic acid production by microbial fermentation processes
journal, November 2013


High-temperature fermentation: how can processes for ethanol production at high temperatures become superior to the traditional process using mesophilic yeast?
journal, October 2009

  • Abdel-Banat, Babiker M. A.; Hoshida, Hisashi; Ano, Akihiko
  • Applied Microbiology and Biotechnology, Vol. 85, Issue 4
  • DOI: 10.1007/s00253-009-2248-5

The level of pyruvate-formate lyase controls the shift from homolactic to mixed-acid product formation in Lactococcus lactis
journal, March 2002


The challenge of enzyme cost in the production of lignocellulosic biofuels
journal, November 2011

  • Klein-Marcuschamer, Daniel; Oleskowicz-Popiel, Piotr; Simmons, Blake A.
  • Biotechnology and Bioengineering, Vol. 109, Issue 4
  • DOI: 10.1002/bit.24370

Fermentative production of lactic acid from renewable materials: Recent achievements, prospects, and limits
journal, January 2015

  • Wang, Ying; Tashiro, Yukihiro; Sonomoto, Kenji
  • Journal of Bioscience and Bioengineering, Vol. 119, Issue 1
  • DOI: 10.1016/j.jbiosc.2014.06.003

Biobutanol from sweet sorghum bagasse hydrolysate by a hybrid pervaporation process
journal, October 2013


Seed train development for the fermentation of bagasse from sweet sorghum and sugarcane using a simplified fermentation process
journal, January 2013


Consensus and Contention in the Food-Versus-Fuel Debate
journal, October 2014


Biorefinery of sweet sorghum stem
journal, July 2012


Sweet sorghum as a bioenergy crop: Literature review
journal, May 2014


Isolation and Characterization of Acid-Tolerant, Thermophilic Bacteria for Effective Fermentation of Biomass-Derived Sugars to Lactic Acid
journal, May 2006


Efficient Production of Lactic Acid from Sweet Sorghum Juice by a Newly Isolated Lactobacillus salivarius CGMCC 7.75
journal, February 2013

  • Liu, Quanlan; Wang, Shanglong; Zhi, Jian-Fei
  • Indian Journal of Microbiology, Vol. 53, Issue 3
  • DOI: 10.1007/s12088-013-0377-0

Detoxification of Dilute Acid Hydrolysates of Lignocellulose with Lime: DETOXIFICATION OF DILUTE ACID HYDROLYSATES OF LIGNOCELLULOSE WITH LIME
journal, January 2001

  • Martinez, Alfredo; Rodriguez, Maria E.; Wells, Melissa L.
  • Biotechnology Progress, Vol. 17, Issue 2
  • DOI: 10.1021/bp0001720

Processing of materials derived from sweet sorghum for biobased products
journal, May 2012


Simultaneous saccharification and fermentation of cellulose to lactic acid
journal, January 1991

  • Abe, Shin-ichiro; Takagi, Motoyoshi
  • Biotechnology and Bioengineering, Vol. 37, Issue 1
  • DOI: 10.1002/bit.260370113

Poly(lactide) Stereocomplexes: Formation, Structure, Properties, Degradation, and Applications
journal, July 2005


Functionalized Polymers from Lignocellulosic Biomass: State of the Art
journal, May 2013


Works referencing / citing this record:

Efficient l ‐lactic acid production from purified sweet sorghum juice coupled with soybean hydrolysate as nitrogen source by Lactobacillus thermophilus A69 strain
journal, March 2019

  • Tian, Xue‐jiao; Jiang, Ai‐lian; Mao, Yan‐qin
  • Journal of Chemical Technology & Biotechnology, Vol. 94, Issue 6
  • DOI: 10.1002/jctb.5939

Techno-Economic Evaluation of Cellulosic Ethanol Production Based on Pilot Biorefinery Data: a Case Study of Sweet Sorghum Bagasse Processed via L+SScF
journal, April 2018


Potential Use of Bacillus coagulans in the Food Industry
journal, June 2018


Potential Use of Bacillus coagulans in the Food Industry
journal, June 2018