Chloroplast lipid biosynthesis is fine-tuned to thylakoid membrane remodeling during light acclimation
Abstract
Reprogramming metabolism, in addition to modifying the structure and function of the photosynthetic machinery, are crucial for plant acclimation to changing light conditions. One of the key acclimatory responses involves reorganization of the photosynthetic membrane system including changes in thylakoid stacking. Glycerolipids are the main structural component of thylakoids and they are synthesized by two main pathways localized in the plastid and the endoplasmic reticulum (ER), however, the role of lipid metabolism in light acclimation remains largely unknown. We found that fatty acid synthesis, membrane lipid content and the plastid lipid biosynthetic pathway activity were significantly higher in plants grown under low light compared with plants grown under normal light. Plants grown under high light, on the other hand, showed a lower rate of fatty acid synthesis, a higher fatty acid flux through the ER pathway and higher triacylglycerol content. We additionally demonstrated that changes in rates of fatty acid synthesis under different growth light conditions are due to posttranslational regulation of the plastidic acetyl-CoA carboxylase activity. Furthermore, Arabidopsis mutants defective in one of the two glycerolipid biosynthetic pathways displayed altered growth patterns and a severely reduced ability to remodel thylakoid architecture particularly under high light. Overall, this study revealsmore »
- Authors:
-
[1];
[1];
[1]
- Biology Department, Brookhaven National Laboratory, Upton, New York 11973, USA
- Biology Department, Brookhaven National Laboratory, Upton, New York 11973, USA, Biology Department, Brookhaven National Laboratory, Upton, New York 11973, USA
- Publication Date:
- Research Org.:
- Brookhaven National Laboratory (BNL), Upton, NY (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1831301
- Alternate Identifier(s):
- OSTI ID: 1706605; OSTI ID: 1784481; OSTI ID: 1785110
- Report Number(s):
- BNL-220578-2020-JAAM; BNL-221407-2021-JAAM; BNL-221406-2021-JAAM
Journal ID: ISSN 0032-0889
- Grant/Contract Number:
- SC0012704
- Resource Type:
- Published Article
- Journal Name:
- Plant Physiology (Bethesda)
- Additional Journal Information:
- Journal Name: Plant Physiology (Bethesda) Journal Volume: 185 Journal Issue: 1; Journal ID: ISSN 0032-0889
- Publisher:
- Oxford University Press
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Yu, Linhui, Fan, Jilian, Zhou, Chao, and Xu, Changcheng. Chloroplast lipid biosynthesis is fine-tuned to thylakoid membrane remodeling during light acclimation. United States: N. p., 2020.
Web. doi:10.1093/plphys/kiaa013.
Yu, Linhui, Fan, Jilian, Zhou, Chao, & Xu, Changcheng. Chloroplast lipid biosynthesis is fine-tuned to thylakoid membrane remodeling during light acclimation. United States. https://doi.org/10.1093/plphys/kiaa013
Yu, Linhui, Fan, Jilian, Zhou, Chao, and Xu, Changcheng. Wed .
"Chloroplast lipid biosynthesis is fine-tuned to thylakoid membrane remodeling during light acclimation". United States. https://doi.org/10.1093/plphys/kiaa013.
@article{osti_1831301,
title = {Chloroplast lipid biosynthesis is fine-tuned to thylakoid membrane remodeling during light acclimation},
author = {Yu, Linhui and Fan, Jilian and Zhou, Chao and Xu, Changcheng},
abstractNote = {Reprogramming metabolism, in addition to modifying the structure and function of the photosynthetic machinery, are crucial for plant acclimation to changing light conditions. One of the key acclimatory responses involves reorganization of the photosynthetic membrane system including changes in thylakoid stacking. Glycerolipids are the main structural component of thylakoids and they are synthesized by two main pathways localized in the plastid and the endoplasmic reticulum (ER), however, the role of lipid metabolism in light acclimation remains largely unknown. We found that fatty acid synthesis, membrane lipid content and the plastid lipid biosynthetic pathway activity were significantly higher in plants grown under low light compared with plants grown under normal light. Plants grown under high light, on the other hand, showed a lower rate of fatty acid synthesis, a higher fatty acid flux through the ER pathway and higher triacylglycerol content. We additionally demonstrated that changes in rates of fatty acid synthesis under different growth light conditions are due to posttranslational regulation of the plastidic acetyl-CoA carboxylase activity. Furthermore, Arabidopsis mutants defective in one of the two glycerolipid biosynthetic pathways displayed altered growth patterns and a severely reduced ability to remodel thylakoid architecture particularly under high light. Overall, this study reveals how plants fine-tune fatty acid and glycerolipid biosynthesis to cellular metabolic needs in response to long-term changes in light conditions, highlighting the importance of lipid metabolism in light acclimation.},
doi = {10.1093/plphys/kiaa013},
journal = {Plant Physiology (Bethesda)},
number = 1,
volume = 185,
place = {United States},
year = {Wed Nov 18 00:00:00 EST 2020},
month = {Wed Nov 18 00:00:00 EST 2020}
}
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