Ultra-High-Resolution Ion Mobility Separations Over Extended Path Lengths and Mobility Ranges Achieved using a Multilevel Structures for Lossless Ion Manipulations Module
Abstract
Over the last few years, structures for lossless ion manipulations (SLIM) have used radiofrequency (RF) fields to confine ions and traveling wave (TW) electric fields to move ions over long serpentine ion paths, and have also allowed for extended serpentine ultralong paths with extended routing (SUPER) separations that can be further enhanced by routing ions through multiple passages through the same path. Such SLIM SUPER ‘multi-pass’ separations provide unprecedentedly high ion mobility resolving powers but are ultimately limited in their ion mobility range to prevent ion ‘lapping’. To achieve ultrahigh resolution separations over a broad mobility range, in this work we have developed a new multilevel SLIM possessing multiple stacked serpentine paths. Ions are transferred between SLIM levels through apertures in the SLIM surfaces. The initial multilevel SLIM module incorporates four levels and three inter-level passages, providing a total path length of 43.2 m. Additionally, using the full path length and helium buffer gas, high resolution separations were achieved for Agilent tuning mixture phosphazene ions over a broad mobility range (K0 ˜ 3.0 to 1.2 cm2/(V*s). High sensitivity was achieved using ‘in-SLIM’ ion accumulation over an extended trapping region of the first SLIM level. High transmission efficiency of ions overmore »
- Authors:
-
[1];
[1];
[1];
[1]
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
- GAA Custom Engineering, LLC, Benton City, WA (United States)
- Publication Date:
- Research Org.:
- Pacific Northwest National Lab. (PNNL), Richland, WA (United States)
- Sponsoring Org.:
- USDOE; National Cancer Institute (NCI); National Institute of General Medical Sciences
- OSTI Identifier:
- 1642387
- Report Number(s):
- PNNL-SA-152421
Journal ID: ISSN 0003-2700
- Grant/Contract Number:
- AC05-76RL01830; R33CA21769; R01 GM130709-01; P41 GM103493-15
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Analytical Chemistry
- Additional Journal Information:
- Journal Volume: 92; Journal Issue: 11; Journal ID: ISSN 0003-2700
- Publisher:
- American Chemical Society (ACS)
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; complex mixture, ion mobility, mass spectrometry, mobility range, SLIM, SLIM SUPER, ultrahigh resolution, multilevel; mixtures; peptides and proteins; power; electrodes; ions
Citation Formats
Hollerbach, Adam L., Li, Ailin, Prabhakaran, Aneesh, Nagy, Gabe, Harrilal, Christopher P., Conant, Christopher R., Norheim, Randolph V., Schimelfenig, Colby E., Anderson, Gordon A., Garimella, Sandilya V. B., Smith, Richard D., and Ibrahim, Yehia M. Ultra-High-Resolution Ion Mobility Separations Over Extended Path Lengths and Mobility Ranges Achieved using a Multilevel Structures for Lossless Ion Manipulations Module. United States: N. p., 2020.
Web. doi:10.1021/acs.analchem.0c01397.
Hollerbach, Adam L., Li, Ailin, Prabhakaran, Aneesh, Nagy, Gabe, Harrilal, Christopher P., Conant, Christopher R., Norheim, Randolph V., Schimelfenig, Colby E., Anderson, Gordon A., Garimella, Sandilya V. B., Smith, Richard D., & Ibrahim, Yehia M. Ultra-High-Resolution Ion Mobility Separations Over Extended Path Lengths and Mobility Ranges Achieved using a Multilevel Structures for Lossless Ion Manipulations Module. United States. https://doi.org/10.1021/acs.analchem.0c01397
Hollerbach, Adam L., Li, Ailin, Prabhakaran, Aneesh, Nagy, Gabe, Harrilal, Christopher P., Conant, Christopher R., Norheim, Randolph V., Schimelfenig, Colby E., Anderson, Gordon A., Garimella, Sandilya V. B., Smith, Richard D., and Ibrahim, Yehia M. Fri .
"Ultra-High-Resolution Ion Mobility Separations Over Extended Path Lengths and Mobility Ranges Achieved using a Multilevel Structures for Lossless Ion Manipulations Module". United States. https://doi.org/10.1021/acs.analchem.0c01397. https://www.osti.gov/servlets/purl/1642387.
@article{osti_1642387,
title = {Ultra-High-Resolution Ion Mobility Separations Over Extended Path Lengths and Mobility Ranges Achieved using a Multilevel Structures for Lossless Ion Manipulations Module},
author = {Hollerbach, Adam L. and Li, Ailin and Prabhakaran, Aneesh and Nagy, Gabe and Harrilal, Christopher P. and Conant, Christopher R. and Norheim, Randolph V. and Schimelfenig, Colby E. and Anderson, Gordon A. and Garimella, Sandilya V. B. and Smith, Richard D. and Ibrahim, Yehia M.},
abstractNote = {Over the last few years, structures for lossless ion manipulations (SLIM) have used radiofrequency (RF) fields to confine ions and traveling wave (TW) electric fields to move ions over long serpentine ion paths, and have also allowed for extended serpentine ultralong paths with extended routing (SUPER) separations that can be further enhanced by routing ions through multiple passages through the same path. Such SLIM SUPER ‘multi-pass’ separations provide unprecedentedly high ion mobility resolving powers but are ultimately limited in their ion mobility range to prevent ion ‘lapping’. To achieve ultrahigh resolution separations over a broad mobility range, in this work we have developed a new multilevel SLIM possessing multiple stacked serpentine paths. Ions are transferred between SLIM levels through apertures in the SLIM surfaces. The initial multilevel SLIM module incorporates four levels and three inter-level passages, providing a total path length of 43.2 m. Additionally, using the full path length and helium buffer gas, high resolution separations were achieved for Agilent tuning mixture phosphazene ions over a broad mobility range (K0 ˜ 3.0 to 1.2 cm2/(V*s). High sensitivity was achieved using ‘in-SLIM’ ion accumulation over an extended trapping region of the first SLIM level. High transmission efficiency of ions over a broad mobility range (e.g. K0 ˜ 3.0 to 1.67 cm2/(V*s)) was achieved, with transmission efficiency rolling off for the lower mobility ions (e.g. K0 ˜ 1.2 cm2/(V*s)). Resolving powers of up to ~400 were achievable using all four ion levels to separate reverse peptides (SDGRG1+ and GRGDS1+). A complex mixture of phosphopeptides showed similar coverage could be achieved using one or all four SLIM levels, and doubly-charged phosphosite isomers not significantly separated using one SLIM level were well resolved when four levels were used. The new multilevel SLIM technology thus enables significantly wider mobility range ultrahigh-resolution ion mobility separations and expands on the ability of SLIM to obtain improved separations of complex mixtures.},
doi = {10.1021/acs.analchem.0c01397},
journal = {Analytical Chemistry},
number = 11,
volume = 92,
place = {United States},
year = {Fri May 08 00:00:00 EDT 2020},
month = {Fri May 08 00:00:00 EDT 2020}
}
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