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Title: Serpentine Ultralong Path with Extended Routing (SUPER) High Resolution Traveling Wave Ion Mobility-MS using Structures for Lossless Ion Manipulations

Abstract

Ion mobility (IM) separations have a broad range of analytical applications, but insufficient resolution limits many applications. Here we report on traveling wave (TW) ion mobility (IM) separations in a Serpentine Ultra-long Path with Extended Routing (SUPER) Structures for Lossless Ion Manipulations (SLIM) module in conjunction with mass spectrometry (MS). The extended routing utilized multiple passes was facilitated by the introduction of a lossless ion switch at the end of the ion path that either directed ions to the MS detector or to another pass through the serpentine separation region, providing theoretically unlimited TWIM path lengths. Ions were confined in the SLIM by rf fields in conjunction with a DC guard bias, enabling essentially lossless TW transmission over greatly extended paths (e.g., ~1094 meters over 81 passes through the 13.5 m serpentine path). In this multi-pass SUPER TWIM provided resolution approximately proportional to the square root of the number of passes (or path length). More than 30-fold higher IM resolution for Agilent tuning mix m/z 622 and 922 ions (~340 vs. ~10) was achieved for 40 passes compared to commercially available drift tube IM and other TWIM-based platforms. An initial evaluation of the isomeric sugars Lacto-N-hexaose and Lacto-N-neohexaose showed themore » isomeric structures to be baseline resolved, and a new conformational feature for Lacto-N-neohexaose was revealed after 9 passes. The new SLIM SUPER high resolution TWIM platform has broad utility in conjunction with MS and is expected to enable a broad range of previously challenging or intractable separations.« less

Authors:
; ; ; ; ORCiD logo; ; ; ; ; ORCiD logo; ORCiD logo; ORCiD logo
Publication Date:
Research Org.:
Pacific Northwest National Laboratory (PNNL), Richland, WA (US), Environmental Molecular Sciences Laboratory (EMSL)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
OSTI Identifier:
1361955
Report Number(s):
PNNL-SA-123438
Journal ID: ISSN 0003-2700; 49531; 48680; KP1601010
DOE Contract Number:  
AC05-76RL01830
Resource Type:
Journal Article
Resource Relation:
Journal Name: Analytical Chemistry; Journal Volume: 89; Journal Issue: 8
Country of Publication:
United States
Language:
English
Subject:
Environmental Molecular Sciences Laboratory

Citation Formats

Deng, Liulin, Webb, Ian K., Garimella, Sandilya V. B., Hamid, Ahmed M., Zheng, Xueyun, Norheim, Randolph V., Prost, Spencer A., Anderson, Gordon A., Sandoval, Jeremy A., Baker, Erin S., Ibrahim, Yehia M., and Smith, Richard D.. Serpentine Ultralong Path with Extended Routing (SUPER) High Resolution Traveling Wave Ion Mobility-MS using Structures for Lossless Ion Manipulations. United States: N. p., 2017. Web. doi:10.1021/acs.analchem.7b00185.
Deng, Liulin, Webb, Ian K., Garimella, Sandilya V. B., Hamid, Ahmed M., Zheng, Xueyun, Norheim, Randolph V., Prost, Spencer A., Anderson, Gordon A., Sandoval, Jeremy A., Baker, Erin S., Ibrahim, Yehia M., & Smith, Richard D.. Serpentine Ultralong Path with Extended Routing (SUPER) High Resolution Traveling Wave Ion Mobility-MS using Structures for Lossless Ion Manipulations. United States. doi:10.1021/acs.analchem.7b00185.
Deng, Liulin, Webb, Ian K., Garimella, Sandilya V. B., Hamid, Ahmed M., Zheng, Xueyun, Norheim, Randolph V., Prost, Spencer A., Anderson, Gordon A., Sandoval, Jeremy A., Baker, Erin S., Ibrahim, Yehia M., and Smith, Richard D.. Wed . "Serpentine Ultralong Path with Extended Routing (SUPER) High Resolution Traveling Wave Ion Mobility-MS using Structures for Lossless Ion Manipulations". United States. doi:10.1021/acs.analchem.7b00185.
@article{osti_1361955,
title = {Serpentine Ultralong Path with Extended Routing (SUPER) High Resolution Traveling Wave Ion Mobility-MS using Structures for Lossless Ion Manipulations},
author = {Deng, Liulin and Webb, Ian K. and Garimella, Sandilya V. B. and Hamid, Ahmed M. and Zheng, Xueyun and Norheim, Randolph V. and Prost, Spencer A. and Anderson, Gordon A. and Sandoval, Jeremy A. and Baker, Erin S. and Ibrahim, Yehia M. and Smith, Richard D.},
abstractNote = {Ion mobility (IM) separations have a broad range of analytical applications, but insufficient resolution limits many applications. Here we report on traveling wave (TW) ion mobility (IM) separations in a Serpentine Ultra-long Path with Extended Routing (SUPER) Structures for Lossless Ion Manipulations (SLIM) module in conjunction with mass spectrometry (MS). The extended routing utilized multiple passes was facilitated by the introduction of a lossless ion switch at the end of the ion path that either directed ions to the MS detector or to another pass through the serpentine separation region, providing theoretically unlimited TWIM path lengths. Ions were confined in the SLIM by rf fields in conjunction with a DC guard bias, enabling essentially lossless TW transmission over greatly extended paths (e.g., ~1094 meters over 81 passes through the 13.5 m serpentine path). In this multi-pass SUPER TWIM provided resolution approximately proportional to the square root of the number of passes (or path length). More than 30-fold higher IM resolution for Agilent tuning mix m/z 622 and 922 ions (~340 vs. ~10) was achieved for 40 passes compared to commercially available drift tube IM and other TWIM-based platforms. An initial evaluation of the isomeric sugars Lacto-N-hexaose and Lacto-N-neohexaose showed the isomeric structures to be baseline resolved, and a new conformational feature for Lacto-N-neohexaose was revealed after 9 passes. The new SLIM SUPER high resolution TWIM platform has broad utility in conjunction with MS and is expected to enable a broad range of previously challenging or intractable separations.},
doi = {10.1021/acs.analchem.7b00185},
journal = {Analytical Chemistry},
number = 8,
volume = 89,
place = {United States},
year = {Wed Apr 05 00:00:00 EDT 2017},
month = {Wed Apr 05 00:00:00 EDT 2017}
}