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Title: Comparative evaluation of Populus variants total sugar release and structural features following pretreatment and digestion by two distinct biological systems

Abstract

Populus natural variants have been shown to realize a broad range of sugar yields during saccharification, however, the structural features responsible for higher sugar release from natural variants are not clear. In addition, the sugar release patterns resulting from digestion with two distinct biological systems, fungal enzymes and Clostridium thermocellum, have yet to be evaluated and compared. This study evaluates the effect of structural features of three natural variant Populus lines, which includes the line BESC standard, with respect to the overall process of sugar release for two different biological systems.

Authors:
; ; ; ; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Biological and Environmental Research (BER)
OSTI Identifier:
1618710
Alternate Identifier(s):
OSTI ID: 1412034
Grant/Contract Number:  
PS02-06ER64304; AC05-00OR22725
Resource Type:
Published Article
Journal Name:
Biotechnology for Biofuels
Additional Journal Information:
Journal Name: Biotechnology for Biofuels Journal Volume: 10 Journal Issue: 1; Journal ID: ISSN 1754-6834
Publisher:
Springer Science + Business Media
Country of Publication:
Netherlands
Language:
English
Subject:
09 BIOMASS FUELS; Populus; Natural variants; Hydrothermal pretreatment; Structural changes; Enzymatic hydrolysis; Consolidated bioprocessing; Clostridium thermocellum

Citation Formats

Thomas, Vanessa A., Kothari, Ninad, Bhagia, Samarthya, Akinosho, Hannah, Li, Mi, Pu, Yunqiao, Yoo, Chang Geun, Pattathil, Sivakumar, Hahn, Michael G., Raguaskas, Arthur J., Wyman, Charles E., and Kumar, Rajeev. Comparative evaluation of Populus variants total sugar release and structural features following pretreatment and digestion by two distinct biological systems. Netherlands: N. p., 2017. Web. doi:10.1186/s13068-017-0975-x.
Thomas, Vanessa A., Kothari, Ninad, Bhagia, Samarthya, Akinosho, Hannah, Li, Mi, Pu, Yunqiao, Yoo, Chang Geun, Pattathil, Sivakumar, Hahn, Michael G., Raguaskas, Arthur J., Wyman, Charles E., & Kumar, Rajeev. Comparative evaluation of Populus variants total sugar release and structural features following pretreatment and digestion by two distinct biological systems. Netherlands. https://doi.org/10.1186/s13068-017-0975-x
Thomas, Vanessa A., Kothari, Ninad, Bhagia, Samarthya, Akinosho, Hannah, Li, Mi, Pu, Yunqiao, Yoo, Chang Geun, Pattathil, Sivakumar, Hahn, Michael G., Raguaskas, Arthur J., Wyman, Charles E., and Kumar, Rajeev. Thu . "Comparative evaluation of Populus variants total sugar release and structural features following pretreatment and digestion by two distinct biological systems". Netherlands. https://doi.org/10.1186/s13068-017-0975-x.
@article{osti_1618710,
title = {Comparative evaluation of Populus variants total sugar release and structural features following pretreatment and digestion by two distinct biological systems},
author = {Thomas, Vanessa A. and Kothari, Ninad and Bhagia, Samarthya and Akinosho, Hannah and Li, Mi and Pu, Yunqiao and Yoo, Chang Geun and Pattathil, Sivakumar and Hahn, Michael G. and Raguaskas, Arthur J. and Wyman, Charles E. and Kumar, Rajeev},
abstractNote = {Populus natural variants have been shown to realize a broad range of sugar yields during saccharification, however, the structural features responsible for higher sugar release from natural variants are not clear. In addition, the sugar release patterns resulting from digestion with two distinct biological systems, fungal enzymes and Clostridium thermocellum, have yet to be evaluated and compared. This study evaluates the effect of structural features of three natural variant Populus lines, which includes the line BESC standard, with respect to the overall process of sugar release for two different biological systems.},
doi = {10.1186/s13068-017-0975-x},
journal = {Biotechnology for Biofuels},
number = 1,
volume = 10,
place = {Netherlands},
year = {Thu Nov 30 00:00:00 EST 2017},
month = {Thu Nov 30 00:00:00 EST 2017}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
https://doi.org/10.1186/s13068-017-0975-x

Citation Metrics:
Cited by: 16 works
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Works referenced in this record:

The characterization of pretreated lignocellulosic substrates prior to enzymatic hydrolysis, part 1: A modified Simons' staining technique
journal, September 2008

  • Chandra, Richard; Ewanick, Shannon; Hsieh, Carmen
  • Biotechnology Progress, Vol. 24, Issue 5
  • DOI: 10.1002/btpr.33

Two-year field analysis of reduced recalcitrance transgenic switchgrass
journal, April 2014

  • Baxter, Holly L.; Mazarei, Mitra; Labbe, Nicole
  • Plant Biotechnology Journal, Vol. 12, Issue 7
  • DOI: 10.1111/pbi.12195

Natural genetic variability reduces recalcitrance in poplar
journal, May 2016

  • Bhagia, Samarthya; Muchero, Wellington; Kumar, Rajeev
  • Biotechnology for Biofuels, Vol. 9, Issue 1
  • DOI: 10.1186/s13068-016-0521-2

Dilute Acid and Autohydrolysis Pretreatment
book, August 2009


Lignin content in natural Populus variants affects sugar release
journal, March 2011

  • Studer, M. H.; DeMartini, J. D.; Davis, M. F.
  • Proceedings of the National Academy of Sciences, Vol. 108, Issue 15, p. 6300-6305
  • DOI: 10.1073/pnas.1009252108

Small-scale and automatable high-throughput compositional analysis of biomass
journal, October 2010

  • DeMartini, Jaclyn D.; Studer, Michael H.; Wyman, Charles E.
  • Biotechnology and Bioengineering, Vol. 108, Issue 2, p. 306-312
  • DOI: 10.1002/bit.22937

Genetic manipulation of lignin reduces recalcitrance and improves ethanol production from switchgrass
journal, February 2011

  • Fu, Chunxiang; Mielenz, Jonathan R.; Xiao, Xirong
  • Proceedings of the National Academy of Sciences, Vol. 108, Issue 9, p. 3803-3808
  • DOI: 10.1073/pnas.1100310108

Immunological Approaches to Plant Cell Wall and Biomass Characterization: Glycome Profiling
book, January 2012


Application of monoclonal antibodies to investigate plant cell wall deconstruction for biofuels production
journal, January 2011

  • DeMartini, Jaclyn D.; Pattathil, Sivakumar; Avci, Utku
  • Energy & Environmental Science, Vol. 4, Issue 10
  • DOI: 10.1039/c1ee02112e

Phenomenological kinetics of complex systems: the development of a generalized severity parameter and its application to lignocellulosics fractionation
journal, April 1992


Clostridium thermocellum releases coumaric acid during degradation of untreated grasses by the action of an unknown enzyme
journal, January 2016

  • Herring, Christopher D.; Thorne, Philip G.; Lynd, Lee R.
  • Applied Microbiology and Biotechnology, Vol. 100, Issue 6
  • DOI: 10.1007/s00253-016-7294-1

Morphological and crystalline characterization of NaOH and NaOCl treated Agave americana L. fiber
journal, March 2012

  • Ben Sghaier, Asma El Oudiani; Chaabouni, Yassin; Msahli, Slah
  • Industrial Crops and Products, Vol. 36, Issue 1
  • DOI: 10.1016/j.indcrop.2011.09.012

The impact of dilute sulfuric acid on the selectivity of xylooligomer depolymerization to monomers
journal, February 2008


Effect of liquid hot water pretreatment severity on properties of hardwood lignin and enzymatic hydrolysis of cellulose: Lignin Effect on Enzyme Hydrolysis of Cellulose
journal, October 2014

  • Ko, Ja Kyong; Kim, Youngmi; Ximenes, Eduardo
  • Biotechnology and Bioengineering, Vol. 112, Issue 2
  • DOI: 10.1002/bit.25349

Direct conversion of plant biomass to ethanol by engineered Caldicellulosiruptor bescii
journal, June 2014

  • Chung, D.; Cha, M.; Guss, A. M.
  • Proceedings of the National Academy of Sciences, Vol. 111, Issue 24, p. 8931-8936
  • DOI: 10.1073/pnas.1402210111

Features of promising technologies for pretreatment of lignocellulosic biomass
journal, April 2005


A Comprehensive Toolkit of Plant Cell Wall Glycan-Directed Monoclonal Antibodies
journal, April 2010

  • Pattathil, S.; Avci, U.; Baldwin, D.
  • Plant Physiology, Vol. 153, Issue 2, p. 514-525
  • DOI: 10.1104/pp.109.151985

Flowthrough pretreatment with very dilute acid provides insights into high lignin contribution to biomass recalcitrance
journal, November 2016


Cellulosic ethanol: status and innovation
journal, June 2017


Recent advances in understanding the role of cellulose accessibility in enzymatic hydrolysis of lignocellulosic substrates
journal, June 2014


The need for biofuels as part of a low carbon energy future
journal, June 2015

  • Fulton, Lewis M.; Lynd, Lee R.; Körner, Alexander
  • Biofuels, Bioproducts and Biorefining, Vol. 9, Issue 5
  • DOI: 10.1002/bbb.1559

The challenge of enzyme cost in the production of lignocellulosic biofuels
journal, November 2011

  • Klein-Marcuschamer, Daniel; Oleskowicz-Popiel, Piotr; Simmons, Blake A.
  • Biotechnology and Bioengineering, Vol. 109, Issue 4
  • DOI: 10.1002/bit.24370

Recent progress in consolidated bioprocessing
journal, June 2012

  • Olson, Daniel G.; McBride, John E.; Joe Shaw, A.
  • Current Opinion in Biotechnology, Vol. 23, Issue 3, p. 396-405
  • DOI: 10.1016/j.copbio.2011.11.026

Comparison of enzymatic reactivity of corn stover solids prepared by dilute acid, AFEX™, and ionic liquid pretreatments
journal, January 2014

  • Gao, Xiadi; Kumar, Rajeev; Singh, Seema
  • Biotechnology for Biofuels, Vol. 7, Issue 1
  • DOI: 10.1186/1754-6834-7-71

Comparison of laboratory delignification methods, their selectivity, and impacts on physiochemical characteristics of cellulosic biomass
journal, February 2013


Water sorption in pretreated grasses as a predictor of enzymatic hydrolysis yields
journal, December 2017


Combined sugar yields for dilute sulfuric acid pretreatment of corn stover followed by enzymatic hydrolysis of the remaining solids
journal, December 2005


An In-Depth Understanding of Biomass Recalcitrance Using Natural Poplar Variants as the Feedstock
journal, December 2016


Works referencing / citing this record:

Biomass Recalcitrance in Willow Under Two Biological Conversion Paradigms: Enzymatic Hydrolysis and Anaerobic Digestion
journal, December 2019


Cellulose hydrolysis by Clostridium thermocellum is agnostic to substrate structural properties in contrast to fungal cellulases
journal, January 2019

  • Kothari, Ninad; Bhagia, Samarthya; Zaher, Maher
  • Green Chemistry, Vol. 21, Issue 10
  • DOI: 10.1039/c9gc00262f

Construction of consolidated bio-saccharification biocatalyst and process optimization for highly efficient lignocellulose solubilization
journal, February 2019