Biological lignocellulose solubilization: Comparative evaluation of biocatalysts and enhancement via cotreatment

Paye, Julie M. D.; Guseva, Anna; Hammer, Sarah K.; Gjersing, Erica; Davis, Mark F.; Davison, Brian H.; Olstad, Jessica; Donohoe, Bryon S.; Nguyen, Thanh Yen; Wyman, Charles E.; Pattathil, Sivakumar; Hahn, Michael G.; Lynd, Lee R.

doi:10.1186/s13068-015-0412-y

Title: Biological lignocellulose solubilization: Comparative evaluation of biocatalysts and enhancement via cotreatment

Journal Article · 11 January 2016 · Biotechnology for Biofuels

DOI: https://doi.org/10.1186/s13068-015-0412-y · OSTI ID:1236762

Paye, Julie M. D. ^[1]; Guseva, Anna ^[1]; Hammer, Sarah K. ^[1]; Gjersing, Erica ^[2]; Davis, Mark F. ^[2]; Davison, Brian H. ^[3]; Olstad, Jessica ^[2]; Donohoe, Bryon S. ^[2]; Nguyen, Thanh Yen ^[4]; Wyman, Charles E. ^[4]; Pattathil, Sivakumar ^[5]; Hahn, Michael G. ^[5]; Lynd, Lee R. ^[1]

Dartmouth College, Hanover, NH (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); National Renewable Energy Lab. (NREL), Golden, CO (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of California, Riverside, CA (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Georgia, Athens, GA (United States)

Feedstock recalcitrance is the most important barrier impeding cost-effective production of cellulosic biofuels. Pioneer commercial cellulosic ethanol facilities employ thermochemical pretreatment and addition of fungal cellulase, reflecting the main research emphasis in the field. However, it has been suggested that it may be possible to process cellulosic biomass without thermochemical pretreatment using thermophilic, cellulolytic bacteria. Thus, to further explore this idea, we examine the ability of various biocatalysts to solubilize autoclaved but otherwise unpretreated cellulosic biomass under controlled but not industrial conditions.

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Research Organization:: NREL (National Renewable Energy Laboratory (NREL), Golden, CO (United States))

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC36-08GO28308

OSTI ID:: 1236762

Report Number(s):: NREL/JA--5100-65764

Journal Information:: Biotechnology for Biofuels, Journal Name: Biotechnology for Biofuels Journal Issue: 1 Vol. 9; ISSN 1754-6834

Publisher:: BioMed CentralCopyright Statement

Country of Publication:: United States

Language:: English

References (84)

Enhancement of cellulose accessibility and enzymatic hydrolysis by simultaneous wet milling Kelsey, Rick G.; Shafizadeh, Fred Biotechnology and Bioengineering, Vol. 22, Issue 5 https://doi.org/10.1002/bit.260220511	journal	May 1980
Enhancement of enzymatic hydrolysis by simultaneous attrition of cellulosic substrates Neilson, Martin J.; Kelsey, Rick G.; Shafizadeh, Fred Biotechnology and Bioengineering, Vol. 24, Issue 2 https://doi.org/10.1002/bit.260240204	journal	February 1982
Bioconversion of waste cellulose by using an attrition bioreactor Ryu, S. K.; Lee, J. M. Biotechnology and Bioengineering, Vol. 25, Issue 1 https://doi.org/10.1002/bit.260250106	journal	January 1983
Hydrolysis of dilute acid pretreated mixed hardwood and purified microcrystalline cellulose by cell-free broth fromClostridium thermocellum Lynd, L. R.; Grethlein, H. E. Biotechnology and Bioengineering, Vol. 29, Issue 1 https://doi.org/10.1002/bit.260290114	journal	January 1987
Comparative sugar recovery and fermentation data following pretreatment of poplar wood by leading technologies Wyman, Charles E.; Dale, Bruce E.; Elander, Richard T. Biotechnology Progress, Vol. 25, Issue 2 https://doi.org/10.1002/btpr.142	journal	March 2009
Simultaneous wet ball milling and mild acid hydrolysis of rice hull Zhou, Jinxiang; Chen, Ding; Zhu, Yonghua Journal of Chemical Technology & Biotechnology, Vol. 85, Issue 1 https://doi.org/10.1002/jctb.2270	journal	January 2010
A defined growth medium with very low background carbon for culturing Clostridium thermocellum Holwerda, Evert K.; Hirst, Kyle D.; Lynd, Lee R. Journal of Industrial Microbiology & Biotechnology, Vol. 39, Issue 6 https://doi.org/10.1007/s10295-012-1091-3	journal	February 2012
Summary of findings from the Biomass Refining Consortium for Applied Fundamentals and Innovation (CAFI): corn stover pretreatment Elander, Richard T.; Dale, Bruce E.; Holtzapple, Mark Cellulose, Vol. 16, Issue 4 https://doi.org/10.1007/s10570-009-9308-y	journal	June 2009
Lessons from the cow: What the ruminant animal can teach us about consolidated bioprocessing of cellulosic biomass Weimer, Paul J.; Russell, James B.; Muck, Richard E. Bioresource Technology, Vol. 100, Issue 21 https://doi.org/10.1016/j.biortech.2009.04.075	journal	November 2009
Conversion for Avicel and AFEX pretreated corn stover by Clostridium thermocellum and simultaneous saccharification and fermentation: Insights into microbial conversion of pretreated cellulosic biomass Shao, Xiongjun; Jin, Mingjie; Guseva, Anna Bioresource Technology, Vol. 102, Issue 17 https://doi.org/10.1016/j.biortech.2011.05.021	journal	September 2011
Comparative data on effects of leading pretreatments and enzyme loadings and formulations on sugar yields from different switchgrass sources Wyman, Charles E.; Balan, Venkatesh; Dale, Bruce E. Bioresource Technology, Vol. 102, Issue 24 https://doi.org/10.1016/j.biortech.2011.06.069	journal	December 2011
Efficient saccharification of ammonia soaked rice straw by combination of Clostridium thermocellum cellulosome and Thermoanaerobacter brockii β-glucosidase Waeonukul, Rattiya; Kosugi, Akihiko; Tachaapaikoon, Chakrit Bioresource Technology, Vol. 107 https://doi.org/10.1016/j.biortech.2011.12.126	journal	March 2012
Dry fractionation process as an important step in current and future lignocellulose biorefineries: A review Barakat, Abdellatif; de Vries, Hugo; Rouau, Xavier Bioresource Technology, Vol. 134 https://doi.org/10.1016/j.biortech.2013.01.169	journal	April 2013
Cellulose fermentation by Clostridium thermocellum and a mixed consortium in an automated repetitive batch reactor Reed, Parker T.; Izquierdo, Javier A.; Lynd, Lee R. Bioresource Technology, Vol. 155 https://doi.org/10.1016/j.biortech.2013.12.051	journal	March 2014
Combined pretreatment using alkaline hydrothermal and ball milling to enhance enzymatic hydrolysis of oil palm mesocarp fiber Zakaria, Mohd Rafein; Hirata, Satoshi; Hassan, Mohd Ali Bioresource Technology, Vol. 169 https://doi.org/10.1016/j.biortech.2014.06.095	journal	October 2014
Impact of ball milling on maize (Zea mays L.) stem structural components and on enzymatic hydrolysis of carbohydrates Sipponen, Mika Henrikki; Laakso, Simo; Baumberger, Stéphanie Industrial Crops and Products, Vol. 61 https://doi.org/10.1016/j.indcrop.2014.06.052	journal	November 2014
Degradation of microcrystalline cellulose and non-pretreated plant biomass by a cell-free extracellular cellulase/hemicellulase system from the extreme thermophilic bacterium Caldicellulosiruptor bescii Kanafusa-Shinkai, Sumiyo; Wakayama, Jun'ichi; Tsukamoto, Kazumi Journal of Bioscience and Bioengineering, Vol. 115, Issue 1 https://doi.org/10.1016/j.jbiosc.2012.07.019	journal	January 2013
Quantitative Saccharification of Wood and Cellulose Saeman, Jerome F.; Bubl, Janet L.; Harris, Elwin E. Industrial & Engineering Chemistry Analytical Edition, Vol. 17, Issue 1, p. 35-37 https://doi.org/10.1021/i560137a008	journal	January 1945
Methods for Pretreatment of Lignocellulosic Biomass for Efficient Hydrolysis and Biofuel Production Kumar, Parveen; Barrett, Diane M.; Delwiche, Michael J. Industrial & Engineering Chemistry Research, Vol. 48, Issue 8, p. 3713-3729 https://doi.org/10.1021/ie801542g	journal	April 2009
Carbon-13 NMR and order in cellulose Maciel, Gary E.; Kolodziejski, Waclaw L.; Bertran, Maria S. Macromolecules, Vol. 15, Issue 2 https://doi.org/10.1021/ma00230a097	journal	March 1982
Genome sequencing and analysis of the biomass-degrading fungus Trichoderma reesei (syn. Hypocrea jecorina) Martinez, Diego; Berka, Randy M.; Henrissat, Bernard Nature Biotechnology, Vol. 26, Issue 5 https://doi.org/10.1038/nbt1403	journal	May 2008
Application of monoclonal antibodies to investigate plant cell wall deconstruction for biofuels production DeMartini, Jaclyn D.; Pattathil, Sivakumar; Avci, Utku Energy & Environmental Science, Vol. 4, Issue 10 https://doi.org/10.1039/c1ee02112e	journal	January 2011
Fungal cellulases and complexed cellulosomal enzymes exhibit synergistic mechanisms in cellulose deconstruction Resch, Michael G.; Donohoe, Bryon S.; Baker, John O. Energy & Environmental Science, Vol. 6, Issue 6 https://doi.org/10.1039/c3ee00019b	journal	January 2013
Carbohydrate and lignin are simultaneously solubilized from unpretreated switchgrass by microbial action at high temperature Kataeva, Irina; Foston, Marcus B.; Yang, Sung-Jae Energy & Environmental Science, Vol. 6, Issue 7 https://doi.org/10.1039/c3ee40932e	journal	January 2013
Growth inhibition of Clostridium cellulolyticum by an inefficiently regulated carbon flow Guedon, E.; Desvaux, M.; Payot, S. Microbiology, Vol. 145, Issue 8 https://doi.org/10.1099/13500872-145-8-1831	journal	August 1999
Thermophilic lignocellulose deconstruction Blumer-Schuette, Sara E.; Brown, Steven D.; Sander, Kyle B. FEMS Microbiology Reviews, Vol. 38, Issue 3 https://doi.org/10.1111/1574-6976.12044	journal	May 2014
Comparison of Extracellular Cellulase Activities of Clostridium thermocellum LQRI and Trichoderma reesei QM9414 Ng, Thomas K.; Zeikus, J. G. Applied and Environmental Microbiology, Vol. 42, Issue 2 https://doi.org/10.1128/aem.42.2.231-240.1981	journal	January 1981
Saccharification of Complex Cellulosic Substrates by the Cellulase System from Clostridium thermocellum Johnson, Eric A.; Sakajoh, Mitsuji; Halliwell, Geoffrey Applied and Environmental Microbiology, Vol. 43, Issue 5 https://doi.org/10.1128/aem.43.5.1125-1132.1982	journal	January 1982
Enzymatic Hydrolysis of Cellulose Reese, Elwyn T. Applied Microbiology, Vol. 4, Issue 1 https://doi.org/10.1128/am.4.1.39-45.1956	journal	January 1956
Integrated analysis of hydrothermal flow through pretreatment Archambault-Leger, Veronique; Shao, Xiongjun; Lynd, Lee R. Biotechnology for Biofuels, Vol. 5, Issue 1 https://doi.org/10.1186/1754-6834-5-49	journal	January 2012
Evaluation of the bioconversion of genetically modified switchgrass using simultaneous saccharification and fermentation and a consolidated bioprocessing approach Yee, Kelsey L.; Rodriguez Jr, Miguel; Tschaplinski, Timothy J. Biotechnology for Biofuels, Vol. 5, Issue 1 https://doi.org/10.1186/1754-6834-5-81	journal	January 2012
Comparative analysis of the ability of Clostridium clariflavum strains and Clostridium thermocellumto utilize hemicellulose and unpretreated plant material Izquierdo, Javier A.; Pattathil, Sivakumar; Guseva, Anna Biotechnology for Biofuels, Vol. 7, Issue 1 https://doi.org/10.1186/s13068-014-0136-4	journal	November 2014
Winter rye as a bioenergy feedstock: impact of crop maturity on composition, biological solubilization and potential revenue Shao, Xiongjun; DiMarco, Kay; Richard, Tom L. Biotechnology for Biofuels, Vol. 8, Issue 1 https://doi.org/10.1186/s13068-015-0225-z	journal	January 2015
Enhancing the Enzymatic Hydrolysis of Cellulosic Materials Using Simultaneous Ball Milling Mais, Ursula; Esteghlalian, Ali R.; Saddler, John N. Applied Biochemistry and Biotechnology, Vol. 98-100, Issue 1-9 https://doi.org/10.1385/abab:98-100:1-9:815	journal	January 2002
An in vitro Technique for Estimating Digestibility of Treated and Untreated Wood Mellenberger, R. W.; Satter, L. D.; Millett, M. A. Journal of Animal Science, Vol. 30, Issue 6 https://doi.org/10.2527/jas1970.3061005x	journal	June 1970
Enhancement of cellulose accessibility and enzymatic hydrolysis by simultaneous wet milling Kelsey, Rick G.; Shafizadeh, Fred Biotechnology and Bioengineering, Vol. 22, Issue 5 https://doi.org/10.1002/bit.260220511	journal	May 1980
Partial acid hydrolysis of cellulosic materials as a pretreatment for enzymatic hydrolysis Knappert, Diane; Grethlein, Hans; Converse, Alvin Biotechnology and Bioengineering, Vol. 22, Issue 7, p. 1449-1463 https://doi.org/10.1002/bit.260220711	journal	July 1980
Enhancement of enzymatic hydrolysis by simultaneous attrition of cellulosic substrates Neilson, Martin J.; Kelsey, Rick G.; Shafizadeh, Fred Biotechnology and Bioengineering, Vol. 24, Issue 2 https://doi.org/10.1002/bit.260240204	journal	February 1982
Bioconversion of waste cellulose by using an attrition bioreactor Ryu, S. K.; Lee, J. M. Biotechnology and Bioengineering, Vol. 25, Issue 1 https://doi.org/10.1002/bit.260250106	journal	January 1983
Hydrolysis of dilute acid pretreated mixed hardwood and purified microcrystalline cellulose by cell-free broth fromClostridium thermocellum Lynd, L. R.; Grethlein, H. E. Biotechnology and Bioengineering, Vol. 29, Issue 1 https://doi.org/10.1002/bit.260290114	journal	January 1987
Comparative sugar recovery and fermentation data following pretreatment of poplar wood by leading technologies Wyman, Charles E.; Dale, Bruce E.; Elander, Richard T. Biotechnology Progress, Vol. 25, Issue 2 https://doi.org/10.1002/btpr.142	journal	March 2009
Simultaneous wet ball milling and mild acid hydrolysis of rice hull Zhou, Jinxiang; Chen, Ding; Zhu, Yonghua Journal of Chemical Technology & Biotechnology, Vol. 85, Issue 1 https://doi.org/10.1002/jctb.2270	journal	January 2010
Enhancing the Enzymatic Hydrolysis of Cellulosic Materials Using Simultaneous Ball Milling Mais, Ursula; Esteghlalian, Ali R.; Saddler, John N. Biotechnology for Fuels and Chemicals https://doi.org/10.1007/978-1-4612-0119-9_66	book	January 2002
Continuous attrition bioreactor with enzyme recycling for the bioconversion of cellulose Lee, J. M.; Wolf, J. H. Applied Biochemistry and Biotechnology, Vol. 18, Issue 1 https://doi.org/10.1007/BF02930826	journal	August 1988
Development of a low-cost fermentation medium for ethanol production from biomass Kadam, K. L.; Newman, M. M. Applied Microbiology and Biotechnology, Vol. 47, Issue 6 https://doi.org/10.1007/s002530050985	journal	June 1997
A defined growth medium with very low background carbon for culturing Clostridium thermocellum Holwerda, Evert K.; Hirst, Kyle D.; Lynd, Lee R. Journal of Industrial Microbiology & Biotechnology, Vol. 39, Issue 6 https://doi.org/10.1007/s10295-012-1091-3	journal	February 2012
Summary of findings from the Biomass Refining Consortium for Applied Fundamentals and Innovation (CAFI): corn stover pretreatment Elander, Richard T.; Dale, Bruce E.; Holtzapple, Mark Cellulose, Vol. 16, Issue 4 https://doi.org/10.1007/s10570-009-9308-y	journal	June 2009
Anaerobic digestion and dewatering characteristics of mechanically disintegrated excess sludge Kopp, Julia; Müller, Johannes; Dichtl, Norbert Water Science and Technology, Vol. 36, Issue 11, 129–136 https://doi.org/10.1016/S0273-1223(97)00694-X	journal	January 1997
Development of switchgrass (Panicum virgatum) as a bioenergy feedstock in the United States McLaughlin, Samuel B.; Adams Kszos, Lynn Biomass and Bioenergy, Vol. 28, Issue 6 https://doi.org/10.1016/j.biombioe.2004.05.006	journal	June 2005
Lessons from the cow: What the ruminant animal can teach us about consolidated bioprocessing of cellulosic biomass Weimer, Paul J.; Russell, James B.; Muck, Richard E. Bioresource Technology, Vol. 100, Issue 21 https://doi.org/10.1016/j.biortech.2009.04.075	journal	November 2009
Comparative material balances around pretreatment technologies for the conversion of switchgrass to soluble sugars Garlock, Rebecca J.; Balan, Venkatesh; Dale, Bruce E. Bioresource Technology, Vol. 102, Issue 24 https://doi.org/10.1016/j.biortech.2011.04.002	journal	December 2011
Conversion for Avicel and AFEX pretreated corn stover by Clostridium thermocellum and simultaneous saccharification and fermentation: Insights into microbial conversion of pretreated cellulosic biomass Shao, Xiongjun; Jin, Mingjie; Guseva, Anna Bioresource Technology, Vol. 102, Issue 17 https://doi.org/10.1016/j.biortech.2011.05.021	journal	September 2011
Comparative data on effects of leading pretreatments and enzyme loadings and formulations on sugar yields from different switchgrass sources Wyman, Charles E.; Balan, Venkatesh; Dale, Bruce E. Bioresource Technology, Vol. 102, Issue 24 https://doi.org/10.1016/j.biortech.2011.06.069	journal	December 2011
Efficient saccharification of ammonia soaked rice straw by combination of Clostridium thermocellum cellulosome and Thermoanaerobacter brockii β-glucosidase Waeonukul, Rattiya; Kosugi, Akihiko; Tachaapaikoon, Chakrit Bioresource Technology, Vol. 107 https://doi.org/10.1016/j.biortech.2011.12.126	journal	March 2012
Dry fractionation process as an important step in current and future lignocellulose biorefineries: A review Barakat, Abdellatif; de Vries, Hugo; Rouau, Xavier Bioresource Technology, Vol. 134 https://doi.org/10.1016/j.biortech.2013.01.169	journal	April 2013
Cellulose fermentation by Clostridium thermocellum and a mixed consortium in an automated repetitive batch reactor Reed, Parker T.; Izquierdo, Javier A.; Lynd, Lee R. Bioresource Technology, Vol. 155 https://doi.org/10.1016/j.biortech.2013.12.051	journal	March 2014
Combined pretreatment using alkaline hydrothermal and ball milling to enhance enzymatic hydrolysis of oil palm mesocarp fiber Zakaria, Mohd Rafein; Hirata, Satoshi; Hassan, Mohd Ali Bioresource Technology, Vol. 169 https://doi.org/10.1016/j.biortech.2014.06.095	journal	October 2014
Degradation of microcrystalline cellulose and non-pretreated plant biomass by a cell-free extracellular cellulase/hemicellulase system from the extreme thermophilic bacterium Caldicellulosiruptor bescii Kanafusa-Shinkai, Sumiyo; Wakayama, Jun'ichi; Tsukamoto, Kazumi Journal of Bioscience and Bioengineering, Vol. 115, Issue 1 https://doi.org/10.1016/j.jbiosc.2012.07.019	journal	January 2013
Quantitative Saccharification of Wood and Cellulose Saeman, Jerome F.; Bubl, Janet L.; Harris, Elwin E. Industrial & Engineering Chemistry Analytical Edition, Vol. 17, Issue 1, p. 35-37 https://doi.org/10.1021/i560137a008	journal	January 1945
Methods for Pretreatment of Lignocellulosic Biomass for Efficient Hydrolysis and Biofuel Production Kumar, Parveen; Barrett, Diane M.; Delwiche, Michael J. Industrial & Engineering Chemistry Research, Vol. 48, Issue 8, p. 3713-3729 https://doi.org/10.1021/ie801542g	journal	April 2009
Carbon-13 NMR and order in cellulose Maciel, Gary E.; Kolodziejski, Waclaw L.; Bertran, Maria S. Macromolecules, Vol. 15, Issue 2 https://doi.org/10.1021/ma00230a097	journal	March 1982
Genome sequencing and analysis of the biomass-degrading fungus Trichoderma reesei (syn. Hypocrea jecorina) Martinez, Diego; Berka, Randy M.; Henrissat, Bernard Nature Biotechnology, Vol. 26, Issue 5 https://doi.org/10.1038/nbt1403	journal	May 2008
Application of monoclonal antibodies to investigate plant cell wall deconstruction for biofuels production DeMartini, Jaclyn D.; Pattathil, Sivakumar; Avci, Utku Energy & Environmental Science, Vol. 4, Issue 10 https://doi.org/10.1039/c1ee02112e	journal	January 2011
Fungal cellulases and complexed cellulosomal enzymes exhibit synergistic mechanisms in cellulose deconstruction Resch, Michael G.; Donohoe, Bryon S.; Baker, John O. Energy & Environmental Science, Vol. 6, Issue 6 https://doi.org/10.1039/c3ee00019b	journal	January 2013
Carbohydrate and lignin are simultaneously solubilized from unpretreated switchgrass by microbial action at high temperature Kataeva, Irina; Foston, Marcus B.; Yang, Sung-Jae Energy & Environmental Science, Vol. 6, Issue 7 https://doi.org/10.1039/c3ee40932e	journal	January 2013
Effect of rumination on reduction of particle size of rumen digesta by cattle Kennedy, Pm Australian Journal of Agricultural Research, Vol. 36, Issue 6 https://doi.org/10.1071/AR9850819	journal	January 1985
Enzyme-microbe synergy during cellulose hydrolysis by Clostridium thermocellum Lu, Y.; Zhang, Y. -H. P.; Lynd, L. R. Proceedings of the National Academy of Sciences, Vol. 103, Issue 44 https://doi.org/10.1073/pnas.0605381103	journal	October 2006
Growth inhibition of Clostridium cellulolyticum by an inefficiently regulated carbon flow Guedon, E.; Desvaux, M.; Payot, S. Microbiology, Vol. 145, Issue 8 https://doi.org/10.1099/13500872-145-8-1831	journal	August 1999
Thermophilic lignocellulose deconstruction Blumer-Schuette, Sara E.; Brown, Steven D.; Sander, Kyle B. FEMS Microbiology Reviews, Vol. 38, Issue 3 https://doi.org/10.1111/1574-6976.12044	journal	May 2014
Efficient Degradation of Lignocellulosic Plant Biomass, without Pretreatment, by the Thermophilic Anaerobe "Anaerocellum thermophilum" DSM 6725 Yang, Sung-Jae; Kataeva, Irina; Hamilton-Brehm, Scott D. Applied and Environmental Microbiology, Vol. 75, Issue 14, p. 4762-4769 https://doi.org/10.1128/AEM.00236-09	journal	May 2009
Enzymatic Hydrolysis of Cellulose Reese, Elwyn T. Applied Microbiology, Vol. 4, Issue 1 https://doi.org/10.1128/AEM.4.1.39-45.1956	journal	January 1956
Comparison of Extracellular Cellulase Activities of Clostridium thermocellum LQRI and Trichoderma reesei QM9414 Ng, Thomas K.; Zeikus, J. G. Applied and Environmental Microbiology, Vol. 42, Issue 2 https://doi.org/10.1128/AEM.42.2.231-240.1981	journal	January 1981
Saccharification of Complex Cellulosic Substrates by the Cellulase System from Clostridium thermocellum Johnson, Eric A.; Sakajoh, Mitsuji; Halliwell, Geoffrey Applied and Environmental Microbiology, Vol. 43, Issue 5 https://doi.org/10.1128/AEM.43.5.1125-1132.1982	journal	January 1982
Microbial Cellulose Utilization: Fundamentals and Biotechnology Lynd, L. R.; Weimer, P. J.; van Zyl, W. H. Microbiology and Molecular Biology Reviews, Vol. 66, Issue 3, p. 506-577 https://doi.org/10.1128/MMBR.66.3.506-577.2002	journal	September 2002
Microbial Cellulose Utilization: Fundamentals and Biotechnology Lynd, L. R.; Weimer, P. J.; van Zyl, W. H. Microbiology and Molecular Biology Reviews, Vol. 66, Issue 4, p. 739-739 https://doi.org/10.1128/mmbr.66.4.739.2002	journal	December 2002
Integrated analysis of hydrothermal flow through pretreatment Archambault-Leger, Veronique; Shao, Xiongjun; Lynd, Lee R. Biotechnology for Biofuels, Vol. 5, Issue 1 https://doi.org/10.1186/1754-6834-5-49	journal	January 2012
Evaluation of the bioconversion of genetically modified switchgrass using simultaneous saccharification and fermentation and a consolidated bioprocessing approach Yee, Kelsey L.; Rodriguez Jr, Miguel; Tschaplinski, Timothy J. Biotechnology for Biofuels, Vol. 5, Issue 1 https://doi.org/10.1186/1754-6834-5-81	journal	January 2012
Comparative analysis of the ability of Clostridium clariflavum strains and Clostridium thermocellumto utilize hemicellulose and unpretreated plant material Izquierdo, Javier A.; Pattathil, Sivakumar; Guseva, Anna Biotechnology for Biofuels, Vol. 7, Issue 1 https://doi.org/10.1186/s13068-014-0136-4	journal	November 2014
Winter rye as a bioenergy feedstock: impact of crop maturity on composition, biological solubilization and potential revenue Shao, Xiongjun; DiMarco, Kay; Richard, Tom L. Biotechnology for Biofuels, Vol. 8, Issue 1 https://doi.org/10.1186/s13068-015-0225-z	journal	January 2015
Enhancing the Enzymatic Hydrolysis of Cellulosic Materials Using Simultaneous Ball Milling Mais, Ursula; Esteghlalian, Ali R.; Saddler, John N. Applied Biochemistry and Biotechnology, Vol. 98-100, Issue 1-9 https://doi.org/10.1385/ABAB:98-100:1-9:815	journal	January 2002
Anaerobic digestion and dewatering characteristics of mechanically disintegrated excess sludge Kopp, Julia; Müller, Johannes; Dichtl, Norbert Water Science and Technology, Vol. 36, Issue 11 https://doi.org/10.2166/wst.1997.0403	journal	December 1997
An in vitro Technique for Estimating Digestibility of Treated and Untreated Wood Mellenberger, R. W.; Satter, L. D.; Millett, M. A. Journal of Animal Science, Vol. 30, Issue 6 https://doi.org/10.2527/jas1970.3061005x	journal	June 1970
Nutritive Value of Corn Silage as Affected by Maturity and Mechanical Processing: A Contemporary Review Johnson, L.; Harrison, J. H.; Hunt, C. Journal of Dairy Science, Vol. 82, Issue 12 https://doi.org/10.3168/jds.S0022-0302(99)75540-2	journal	December 1999
Biological lignocellulose solubilization: comparative evaluation of biocatalysts and enhancement via cotreatment [Supplemental Data] Paye, Julie; Guseva, Anna; Hammer, Sarah figshare-Supplementary information for journal article at DOI: 10.1186/s13068-015-0412-y, 1 DOCX file (5.42 MB) https://doi.org/10.6084/m9.figshare.c.3609365	figure	January 2016

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The effect of switchgrass loadings on feedstock solubilization and biofuel production by Clostridium thermocellum Verbeke, Tobin J.; Garcia, Gabriela M.; Elkins, James G. Biotechnology for Biofuels, Vol. 10, Issue 1 https://doi.org/10.1186/s13068-017-0917-7	journal	November 2017
Metabolome analysis reveals a role for glyceraldehyde 3-phosphate dehydrogenase in the inhibition of C. thermocellum by ethanol Tian, Liang; Perot, Skyler J.; Stevenson, David Biotechnology for Biofuels, Vol. 10, Issue 1 https://doi.org/10.1186/s13068-017-0961-3	journal	November 2017
Development and characterization of stable anaerobic thermophilic methanogenic microbiomes fermenting switchgrass at decreasing residence times Liang, Xiaoyu; Whitham, Jason M.; Holwerda, Evert K. Biotechnology for Biofuels, Vol. 11, Issue 1 https://doi.org/10.1186/s13068-018-1238-1	journal	September 2018
Multiple levers for overcoming the recalcitrance of lignocellulosic biomass Holwerda, Evert K.; Worthen, Robert S.; Kothari, Ninad Biotechnology for Biofuels, Vol. 12, Issue 1 https://doi.org/10.1186/s13068-019-1353-7	journal	January 2019
Metabolic and evolutionary responses of Clostridium thermocellum to genetic interventions aimed at improving ethanol production Holwerda, Evert K.; Olson, Daniel G.; Ruppertsberger, Natalie M. Biotechnology for Biofuels, Vol. 13, Issue 1 https://doi.org/10.1186/s13068-020-01680-5	journal	March 2020
Utilization of Monosaccharides by Hungateiclostridium thermocellum ATCC 27405 through Adaptive Evolution Ha-Tran, Dung Minh; Nguyen, Trinh Thi My; Lo, Shou-Chen Microorganisms, Vol. 9, Issue 7 https://doi.org/10.3390/microorganisms9071445	journal	July 2021
Lignocellulose solubilization and conversion by extremely thermophilic Caldicellulosiruptor bescii improves by maintaining metabolic activity Straub, Christopher T.; Khatibi, Piyum A.; Otten, Jonathan K. Biotechnology and Bioengineering, Vol. 116, Issue 8 https://doi.org/10.1002/bit.26993	journal	May 2019
Bioethanol from Lignocellulosic Biomass Wyman, Charles E.; Cai, Charles M.; Kumar, Rajeev Encyclopedia of Sustainability Science and Technology https://doi.org/10.1007/978-1-4939-2493-6_521-3	book	January 2017
Bioethanol from Lignocellulosic Biomass Wyman, Charles E.; Cai, Charles M.; Kumar, Rajeev Energy from Organic Materials (Biomass) https://doi.org/10.1007/978-1-4939-7813-7_521	book	July 2018
Thermoanaerobacter species differ in their potential to reduce organic acids to their corresponding alcohols Hitschler, Lisa; Kuntz, Michelle; Langschied, Felix Applied Microbiology and Biotechnology, Vol. 102, Issue 19 https://doi.org/10.1007/s00253-018-9210-3	journal	July 2018
Lignocellulosic biomass: Hurdles and challenges in its valorization Singhvi, Mamata S.; Gokhale, Digambar V. Applied Microbiology and Biotechnology, Vol. 103, Issue 23-24 https://doi.org/10.1007/s00253-019-10212-7	journal	November 2019
Structural features influential to enzymatic hydrolysis of cellulose-solvent-based pretreated pinewood and elmwood for ethanol production Satari, Behzad; Karimi, Keikhosro; Molaverdi, Maryam Bioprocess and Biosystems Engineering, Vol. 41, Issue 2 https://doi.org/10.1007/s00449-017-1863-2	journal	November 2017
Harnessing yeast organelles for metabolic engineering Hammer, Sarah K.; Avalos, José L. Nature Chemical Biology, Vol. 13, Issue 8 https://doi.org/10.1038/nchembio.2429	journal	July 2017
Lignocellulose fermentation and residual solids characterization for senescent switchgrass fermentation by Clostridium thermocellum in the presence and absence of continuous in situ ball-milling Balch, Michael L.; Holwerda, Evert K.; Davis, Mark F. Energy & Environmental Science, Vol. 10, Issue 5 https://doi.org/10.1039/c6ee03748h	journal	January 2017
Nanomechanics of cellulose deformation reveal molecular defects that facilitate natural deconstruction Ciesielski, Peter N.; Wagner, Ryan; Bharadwaj, Vivek S. Proceedings of the National Academy of Sciences https://doi.org/10.1073/pnas.1900161116	journal	April 2019
Cellulosomes localise on the surface of membrane vesicles from the cellulolytic bacterium Clostridium thermocellum Ichikawa, Shunsuke; Ogawa, Satoru; Nishida, Ayami FEMS Microbiology Letters, Vol. 366, Issue 12 https://doi.org/10.1093/femsle/fnz145	journal	June 2019
Global Distribution Patterns and Pangenomic Diversity of the Candidate Phylum “Latescibacteria” (WS3) Farag, Ibrahim F.; Youssef, Noha H.; Elshahed, Mostafa S. Applied and Environmental Microbiology, Vol. 83, Issue 10 https://doi.org/10.1128/aem.00521-17	journal	March 2017
Bioavailability of Carbohydrate Content in Natural and Transgenic Switchgrasses for the Extreme Thermophile Caldicellulosiruptor bescii Zurawski, Jeffrey V.; Khatibi, Piyum A.; Akinosho, Hannah O. Applied and Environmental Microbiology, Vol. 83, Issue 17 https://doi.org/10.1128/aem.00969-17	journal	June 2017
Enhanced depolymerization and utilization of raw lignocellulosic material by co-cultures of Ruminiclostridium thermocellum with hemicellulose-utilizing partners Froese, Alan; Schellenberg, John; Sparling, Richard Canadian Journal of Microbiology, Vol. 65, Issue 4 https://doi.org/10.1139/cjm-2018-0535	journal	April 2019
Strain and bioprocess improvement of a thermophilic anaerobe for the production of ethanol from wood Herring, Christopher D.; Kenealy, William R.; Joe Shaw, A. Biotechnology for Biofuels, Vol. 9, Issue 1 https://doi.org/10.1186/s13068-016-0536-8	journal	June 2016
Engineering a highly active thermophilic β-glucosidase to enhance its pH stability and saccharification performance Xia, Wei; Xu, Xinxin; Qian, Lichun Biotechnology for Biofuels, Vol. 9, Issue 1 https://doi.org/10.1186/s13068-016-0560-8	journal	July 2016
Cellulosic ethanol production via consolidated bioprocessing by a novel thermophilic anaerobic bacterium isolated from a Himalayan hot spring Singh, Nisha; Mathur, Anshu S.; Tuli, Deepak K. Biotechnology for Biofuels, Vol. 10, Issue 1 https://doi.org/10.1186/s13068-017-0756-6	journal	March 2017
Efficient whole-cell-catalyzing cellulose saccharification using engineered Clostridium thermocellum Zhang, Jie; Liu, Shiyue; Li, Renmin Biotechnology for Biofuels, Vol. 10, Issue 1 https://doi.org/10.1186/s13068-017-0796-y	journal	May 2017
Metabolome analysis reveals a role for glyceraldehyde 3-phosphate dehydrogenase in the inhibition of C. thermocellum by ethanol Tian, Liang; Perot, Skyler J.; Stevenson, David Biotechnology for Biofuels, Vol. 10, Issue 1 https://doi.org/10.1186/s13068-017-0961-3	journal	November 2017
Multiple levers for overcoming the recalcitrance of lignocellulosic biomass Holwerda, Evert K.; Worthen, Robert S.; Kothari, Ninad Biotechnology for Biofuels, Vol. 12, Issue 1 https://doi.org/10.1186/s13068-019-1353-7	journal	January 2019
Metabolic and evolutionary responses of Clostridium thermocellum to genetic interventions aimed at improving ethanol production Holwerda, Evert K.; Olson, Daniel G.; Ruppertsberger, Natalie M. Biotechnology for Biofuels, Vol. 13, Issue 1 https://doi.org/10.1186/s13068-020-01680-5	journal	March 2020

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Related Subjects

09 BIOMASS FUELS
59 BASIC BIOLOGICAL SCIENCES
biological solubilization
cotreatment
lignocellulose

Biological lignocellulose solubilization: comparative evaluation of biocatalysts and enhancement via cotreatment [Supplemental Data] Paye, Julie; Guseva, Anna; Hammer, Sarah https://doi.org/10.6084/m9.figshare.c.3609365 The current record is supplemented by this figure	figure	January 2016
Biological lignocellulose solubilization: comparative evaluation of biocatalysts and enhancement via cotreatment Paye, Julie; Guseva, Anna; Hammer, Sarah https://doi.org/10.6084/m9.figshare.c.3609365.v1 The current record is supplemented by this dataset	dataset	January 2016
MOESM1 of Biological lignocellulose solubilization: comparative evaluation of biocatalysts and enhancement via cotreatment Paye, Julie; Guseva, Anna; Hammer, Sarah https://doi.org/10.6084/m9.figshare.c.3609365_d1 The current record is supplemented by this text	text	January 2016
MOESM1 of Biological lignocellulose solubilization: comparative evaluation of biocatalysts and enhancement via cotreatment Paye, Julie; Guseva, Anna; Hammer, Sarah https://doi.org/10.6084/m9.figshare.c.3609365_d1.v1 The current record is supplemented by this dataset	dataset	January 2016