skip to main content

DOE PAGESDOE PAGES

Title: Biological lignocellulose solubilization: Comparative evaluation of biocatalysts and enhancement via cotreatment

Feedstock recalcitrance is the most important barrier impeding cost-effective production of cellulosic biofuels. Pioneer commercial cellulosic ethanol facilities employ thermochemical pretreatment and addition of fungal cellulase, reflecting the main research emphasis in the field. However, it has been suggested that it may be possible to process cellulosic biomass without thermochemical pretreatment using thermophilic, cellulolytic bacteria. Thus, to further explore this idea, we examine the ability of various biocatalysts to solubilize autoclaved but otherwise unpretreated cellulosic biomass under controlled but not industrial conditions.
Authors:
 [1] ;  [1] ;  [1] ;  [2] ;  [2] ;  [3] ;  [2] ;  [2] ;  [4] ;  [4] ;  [5] ;  [5] ;  [1]
  1. Dartmouth College, Hanover, NH (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  2. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); National Renewable Energy Lab. (NREL), Golden, CO (United States)
  3. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  4. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of California, Riverside, CA (United States)
  5. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Georgia, Athens, GA (United States)
Publication Date:
Report Number(s):
NREL/JA-5100-65764
Journal ID: ISSN 1754-6834
Grant/Contract Number:
AC36-08GO28308; AC05-00OR22725
Type:
Accepted Manuscript
Journal Name:
Biotechnology for Biofuels
Additional Journal Information:
Journal Volume: 9; Journal Issue: 1; Related Information: Biotechnology for Biofuels; Journal ID: ISSN 1754-6834
Publisher:
BioMed Central
Research Org:
National Renewable Energy Lab. (NREL), Golden, CO (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC)
Sponsoring Org:
USDOE Office of Science (SC)
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; 59 BASIC BIOLOGICAL SCIENCES; biological solubilization; lignocellulose; cotreatment
OSTI Identifier:
1236762
Alternate Identifier(s):
OSTI ID: 1327645

Paye, Julie M. D., Guseva, Anna, Hammer, Sarah K., Gjersing, Erica, Davis, Mark F., Davison, Brian H., Olstad, Jessica, Donohoe, Bryon S., Nguyen, Thanh Yen, Wyman, Charles E., Pattathil, Sivakumar, Hahn, Michael G., and Lynd, Lee R.. Biological lignocellulose solubilization: Comparative evaluation of biocatalysts and enhancement via cotreatment. United States: N. p., Web. doi:10.1186/s13068-015-0412-y.
Paye, Julie M. D., Guseva, Anna, Hammer, Sarah K., Gjersing, Erica, Davis, Mark F., Davison, Brian H., Olstad, Jessica, Donohoe, Bryon S., Nguyen, Thanh Yen, Wyman, Charles E., Pattathil, Sivakumar, Hahn, Michael G., & Lynd, Lee R.. Biological lignocellulose solubilization: Comparative evaluation of biocatalysts and enhancement via cotreatment. United States. doi:10.1186/s13068-015-0412-y.
Paye, Julie M. D., Guseva, Anna, Hammer, Sarah K., Gjersing, Erica, Davis, Mark F., Davison, Brian H., Olstad, Jessica, Donohoe, Bryon S., Nguyen, Thanh Yen, Wyman, Charles E., Pattathil, Sivakumar, Hahn, Michael G., and Lynd, Lee R.. 2016. "Biological lignocellulose solubilization: Comparative evaluation of biocatalysts and enhancement via cotreatment". United States. doi:10.1186/s13068-015-0412-y. https://www.osti.gov/servlets/purl/1236762.
@article{osti_1236762,
title = {Biological lignocellulose solubilization: Comparative evaluation of biocatalysts and enhancement via cotreatment},
author = {Paye, Julie M. D. and Guseva, Anna and Hammer, Sarah K. and Gjersing, Erica and Davis, Mark F. and Davison, Brian H. and Olstad, Jessica and Donohoe, Bryon S. and Nguyen, Thanh Yen and Wyman, Charles E. and Pattathil, Sivakumar and Hahn, Michael G. and Lynd, Lee R.},
abstractNote = {Feedstock recalcitrance is the most important barrier impeding cost-effective production of cellulosic biofuels. Pioneer commercial cellulosic ethanol facilities employ thermochemical pretreatment and addition of fungal cellulase, reflecting the main research emphasis in the field. However, it has been suggested that it may be possible to process cellulosic biomass without thermochemical pretreatment using thermophilic, cellulolytic bacteria. Thus, to further explore this idea, we examine the ability of various biocatalysts to solubilize autoclaved but otherwise unpretreated cellulosic biomass under controlled but not industrial conditions.},
doi = {10.1186/s13068-015-0412-y},
journal = {Biotechnology for Biofuels},
number = 1,
volume = 9,
place = {United States},
year = {2016},
month = {1}
}

Works referenced in this record:

Microbial Cellulose Utilization: Fundamentals and Biotechnology
journal, September 2002
  • Lynd, L. R.; Weimer, P. J.; van Zyl, W. H.
  • Microbiology and Molecular Biology Reviews, Vol. 66, Issue 3, p. 506-577
  • DOI: 10.1128/MMBR.66.3.506-577.2002

Partial acid hydrolysis of cellulosic materials as a pretreatment for enzymatic hydrolysis
journal, July 1980
  • Knappert, Diane; Grethlein, Hans; Converse, Alvin
  • Biotechnology and Bioengineering, Vol. 22, Issue 7, p. 1449-1463
  • DOI: 10.1002/bit.260220711

Quantitative Saccharification of Wood and Cellulose
journal, January 1945
  • Saeman, Jerome F.; Bubl, Janet L.; Harris, Elwin E.
  • Industrial & Engineering Chemistry Analytical Edition, Vol. 17, Issue 1, p. 35-37
  • DOI: 10.1021/i560137a008

Methods for Pretreatment of Lignocellulosic Biomass for Efficient Hydrolysis and Biofuel Production
journal, April 2009
  • Kumar, Parveen; Barrett, Diane M.; Delwiche, Michael J.
  • Industrial & Engineering Chemistry Research, Vol. 48, Issue 8, p. 3713-3729
  • DOI: 10.1021/ie801542g

Efficient Degradation of Lignocellulosic Plant Biomass, without Pretreatment, by the Thermophilic Anaerobe "Anaerocellum thermophilum" DSM 6725
journal, May 2009
  • Yang, Sung-Jae; Kataeva, Irina; Hamilton-Brehm, Scott D.
  • Applied and Environmental Microbiology, Vol. 75, Issue 14, p. 4762-4769
  • DOI: 10.1128/AEM.00236-09