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Title: Consolidated bioprocessing of Populus using Clostridium (Ruminiclostridium) thermocellum: a case study on the impact of lignin composition and structure

Abstract

Higher ratios of syringyl-to-guaiacyl (S/G) lignin components of Populus were shown to improve sugar release by enzymatic hydrolysis using commercial blends. Cellulolytic microbes are often robust biomass hydrolyzers and may offer cost advantages, however, it is unknown whether their activity can also be significantly influenced by the ratio of different monolignol types in Populus biomass. Hydrolysis and fermentation of autoclaved but otherwise not pretreated Populus trichocarpa by Clostridium thermocellum ATCC 27405 was compared using feedstocks that had similar carbohydrate and total lignin contents but differed in S/G ratios. Populus with an S/G ratio of 2.1 was converted more rapidly and to a greater extent compared to similar biomass that had a ratio of 1.2. For either microbes or commercial enzymes, an approximate 50% relative difference in total solids solubilization was measured for both biomasses, which suggests that the differences and limitations in the microbial breakdown of lignocellulose may be largely from the enzymatic hydrolytic process. Unexpectedly, the reduction in glucan content per gram solid in the residual microbially processed biomass was similar (17 18 %) irrespective of S/G ratio, pointing to a similar mechanism of solubilization that proceeded at different rates. Fermentation metabolome testing did not reveal the release ofmore » known biomass-derived alcohol and aldehyde inhibitors that could explain observed differences in microbial hydrolytic activity. Biomass-derived p-hydroxybenzoic acid was up to nine-fold higher in low S/G ratio biomass fermentations, but was not found to be inhibitory in subsequent test fermentations. Cellulose crystallinity and degree of polymerization did not vary between Populus lines and had minor changes after fermentation. However, lignin molecular weights and cellulose accessibility determined by Simons staining were positively correlated to the S/G content. Higher S/G ratios in Populus biomass lead to longer and more linear lignin chains and greater access to surface cellulosic content by microbe-bound enzymatic complexes. Substrate access limitation is suggested as a primary bottleneck in solubilization of minimally processed Populus, which has important implications for microbial deconstruction of lignocellulose biomass. Lastly, our findings will allow others to examine different Populus lines and to test if similar observations are possible for other plant species.« less

Authors:
; ; ; ; ; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States). BioEnergy Science Center (BESC)
Sponsoring Org.:
USDOE Office of Science (SC), Office of Biological and Environmental Research; USDOE Office of Science (SC)
OSTI Identifier:
1618635
Alternate Identifier(s):
OSTI ID: 1240075; OSTI ID: 1240560
Report Number(s):
NREL/JA-5100-65961
Journal ID: ISSN 1754-6834; 31; PII: 445
Grant/Contract Number:  
Bioenergy Science Center (BESC) which is a U.S. Department of Energy Bioenergy Research Center supported by the Office of Biological and Environmental Research in the DOE Office of Science.; AC36-08GO28308; AC05-00OR22725
Resource Type:
Published Article
Journal Name:
Biotechnology for Biofuels
Additional Journal Information:
Journal Name: Biotechnology for Biofuels Journal Volume: 9 Journal Issue: 1; Journal ID: ISSN 1754-6834
Publisher:
Springer Science + Business Media
Country of Publication:
Netherlands
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANAYLYTICAL CHEMISTRY; lignin; syringyl; guaiacyl; S/G ratio; consolidated bioprocessing; Populus; Clostridium thermocellum; molecular weight; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Dumitrache, Alexandru, Akinosho, Hannah, Rodriguez, Jr., Miguel, Meng, Xianzhi, Yoo, Chang Geun, Natzke, Jace, Engle, Nancy L., Sykes, Robert W., Tschaplinski, Timothy J., Muchero, Wellington, Ragauskas, Arthur J., Davison, Brian H., and Brown, Steven D. Consolidated bioprocessing of Populus using Clostridium (Ruminiclostridium) thermocellum: a case study on the impact of lignin composition and structure. Netherlands: N. p., 2016. Web. https://doi.org/10.1186/s13068-016-0445-x.
Dumitrache, Alexandru, Akinosho, Hannah, Rodriguez, Jr., Miguel, Meng, Xianzhi, Yoo, Chang Geun, Natzke, Jace, Engle, Nancy L., Sykes, Robert W., Tschaplinski, Timothy J., Muchero, Wellington, Ragauskas, Arthur J., Davison, Brian H., & Brown, Steven D. Consolidated bioprocessing of Populus using Clostridium (Ruminiclostridium) thermocellum: a case study on the impact of lignin composition and structure. Netherlands. https://doi.org/10.1186/s13068-016-0445-x
Dumitrache, Alexandru, Akinosho, Hannah, Rodriguez, Jr., Miguel, Meng, Xianzhi, Yoo, Chang Geun, Natzke, Jace, Engle, Nancy L., Sykes, Robert W., Tschaplinski, Timothy J., Muchero, Wellington, Ragauskas, Arthur J., Davison, Brian H., and Brown, Steven D. Thu . "Consolidated bioprocessing of Populus using Clostridium (Ruminiclostridium) thermocellum: a case study on the impact of lignin composition and structure". Netherlands. https://doi.org/10.1186/s13068-016-0445-x.
@article{osti_1618635,
title = {Consolidated bioprocessing of Populus using Clostridium (Ruminiclostridium) thermocellum: a case study on the impact of lignin composition and structure},
author = {Dumitrache, Alexandru and Akinosho, Hannah and Rodriguez, Jr., Miguel and Meng, Xianzhi and Yoo, Chang Geun and Natzke, Jace and Engle, Nancy L. and Sykes, Robert W. and Tschaplinski, Timothy J. and Muchero, Wellington and Ragauskas, Arthur J. and Davison, Brian H. and Brown, Steven D.},
abstractNote = {Higher ratios of syringyl-to-guaiacyl (S/G) lignin components of Populus were shown to improve sugar release by enzymatic hydrolysis using commercial blends. Cellulolytic microbes are often robust biomass hydrolyzers and may offer cost advantages, however, it is unknown whether their activity can also be significantly influenced by the ratio of different monolignol types in Populus biomass. Hydrolysis and fermentation of autoclaved but otherwise not pretreated Populus trichocarpa by Clostridium thermocellum ATCC 27405 was compared using feedstocks that had similar carbohydrate and total lignin contents but differed in S/G ratios. Populus with an S/G ratio of 2.1 was converted more rapidly and to a greater extent compared to similar biomass that had a ratio of 1.2. For either microbes or commercial enzymes, an approximate 50% relative difference in total solids solubilization was measured for both biomasses, which suggests that the differences and limitations in the microbial breakdown of lignocellulose may be largely from the enzymatic hydrolytic process. Unexpectedly, the reduction in glucan content per gram solid in the residual microbially processed biomass was similar (17 18 %) irrespective of S/G ratio, pointing to a similar mechanism of solubilization that proceeded at different rates. Fermentation metabolome testing did not reveal the release of known biomass-derived alcohol and aldehyde inhibitors that could explain observed differences in microbial hydrolytic activity. Biomass-derived p-hydroxybenzoic acid was up to nine-fold higher in low S/G ratio biomass fermentations, but was not found to be inhibitory in subsequent test fermentations. Cellulose crystallinity and degree of polymerization did not vary between Populus lines and had minor changes after fermentation. However, lignin molecular weights and cellulose accessibility determined by Simons staining were positively correlated to the S/G content. Higher S/G ratios in Populus biomass lead to longer and more linear lignin chains and greater access to surface cellulosic content by microbe-bound enzymatic complexes. Substrate access limitation is suggested as a primary bottleneck in solubilization of minimally processed Populus, which has important implications for microbial deconstruction of lignocellulose biomass. Lastly, our findings will allow others to examine different Populus lines and to test if similar observations are possible for other plant species.},
doi = {10.1186/s13068-016-0445-x},
journal = {Biotechnology for Biofuels},
number = 1,
volume = 9,
place = {Netherlands},
year = {2016},
month = {2}
}

Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
https://doi.org/10.1186/s13068-016-0445-x

Citation Metrics:
Cited by: 19 works
Citation information provided by
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Figures / Tables:

Fig. 1 Fig. 1: Top The three primary monolignols (from left to right) that form H, G, and S lignin units (respectively) and the most common linkage in the lignin polymer, the $β$-O-4 ether bond (right-most); Bottom highly resistant 5-5 (left) and $β$-5 (right) lignin linkages facilitated by the G unit

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