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Title: Experimental 3D coherent diffractive imaging from photon-sparse random projections

Abstract

The routine atomic resolution structure determination of single particles is expected to have profound implications for probing structure–function relationships in systems ranging from energy-storage materials to biological molecules. Extremely bright ultrashort-pulse X-ray sources – X-ray free-electron lasers (XFELs) – provide X-rays that can be used to probe ensembles of nearly identical nanoscale particles. When combined with coherent diffractive imaging, these objects can be imaged; however, as the resolution of the images approaches the atomic scale, the measured data are increasingly difficult to obtain and, during an X-ray pulse, the number of photons incident on the 2D detector is much smaller than the number of pixels. This latter concern, the signal `sparsity', materially impedes the application of the method. An experimental analog using a conventional X-ray source is demonstrated and yields signal levels comparable with those expected from single biomolecules illuminated by focused XFEL pulses. The analog experiment provides an invaluable cross check on the fidelity of the reconstructed data that is not available during XFEL experiments. Using these experimental data, it is established that a sparsity of order 1.3 × 10 −3  photons per pixel per frame can be overcome, lending vital insight to the solution of the atomic resolutionmore » XFEL single-particle imaging problem by experimentally demonstrating 3D coherent diffractive imaging from photon-sparse random projections.« less

Authors:
; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ; ;
Publication Date:
Research Org.:
Brookhaven National Lab. (BNL), Upton, NY (United States)
Sponsoring Org.:
USDOE Office of Science (SC), Basic Energy Sciences (BES)
OSTI Identifier:
1617929
Alternate Identifier(s):
OSTI ID: 1504880
Report Number(s):
BNL-211503-2019-JAAM
Journal ID: ISSN 2052-2525; IUCRAJ; PII: S2052252519002781
Grant/Contract Number:  
SC0004079; SC0016035; SC0017631; AC02-76SF00515; SC0012704
Resource Type:
Published Article
Journal Name:
IUCrJ
Additional Journal Information:
Journal Name: IUCrJ Journal Volume: 6 Journal Issue: 3; Journal ID: ISSN 2052-2525
Publisher:
International Union of Crystallography (IUCr)
Country of Publication:
United Kingdom
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; coherent X-ray diffractive imaging (CXDI); X-ray free-electron lasers; XFELs; phase problem; single particles

Citation Formats

Giewekemeyer, K., Aquila, A., Loh, N. -T. D., Chushkin, Y., Shanks, K. S., Weiss, J. T., Tate, M. W., Philipp, H. T., Stern, S., Vagovic, P., Mehrjoo, M., Teo, C., Barthelmess, M., Zontone, F., Chang, C., Tiberio, R. C., Sakdinawat, A., Williams, G. J., Gruner, S. M., and Mancuso, A. P. Experimental 3D coherent diffractive imaging from photon-sparse random projections. United Kingdom: N. p., 2019. Web. doi:10.1107/S2052252519002781.
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Giewekemeyer, K., Aquila, A., Loh, N. -T. D., Chushkin, Y., Shanks, K. S., Weiss, J. T., Tate, M. W., Philipp, H. T., Stern, S., Vagovic, P., Mehrjoo, M., Teo, C., Barthelmess, M., Zontone, F., Chang, C., Tiberio, R. C., Sakdinawat, A., Williams, G. J., Gruner, S. M., & Mancuso, A. P. Experimental 3D coherent diffractive imaging from photon-sparse random projections. United Kingdom. https://doi.org/10.1107/S2052252519002781
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Giewekemeyer, K., Aquila, A., Loh, N. -T. D., Chushkin, Y., Shanks, K. S., Weiss, J. T., Tate, M. W., Philipp, H. T., Stern, S., Vagovic, P., Mehrjoo, M., Teo, C., Barthelmess, M., Zontone, F., Chang, C., Tiberio, R. C., Sakdinawat, A., Williams, G. J., Gruner, S. M., and Mancuso, A. P. Wed . "Experimental 3D coherent diffractive imaging from photon-sparse random projections". United Kingdom. https://doi.org/10.1107/S2052252519002781.
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@article{osti_1617929,
title = {Experimental 3D coherent diffractive imaging from photon-sparse random projections},
author = {Giewekemeyer, K. and Aquila, A. and Loh, N. -T. D. and Chushkin, Y. and Shanks, K. S. and Weiss, J. T. and Tate, M. W. and Philipp, H. T. and Stern, S. and Vagovic, P. and Mehrjoo, M. and Teo, C. and Barthelmess, M. and Zontone, F. and Chang, C. and Tiberio, R. C. and Sakdinawat, A. and Williams, G. J. and Gruner, S. M. and Mancuso, A. P.},
abstractNote = {The routine atomic resolution structure determination of single particles is expected to have profound implications for probing structure–function relationships in systems ranging from energy-storage materials to biological molecules. Extremely bright ultrashort-pulse X-ray sources – X-ray free-electron lasers (XFELs) – provide X-rays that can be used to probe ensembles of nearly identical nanoscale particles. When combined with coherent diffractive imaging, these objects can be imaged; however, as the resolution of the images approaches the atomic scale, the measured data are increasingly difficult to obtain and, during an X-ray pulse, the number of photons incident on the 2D detector is much smaller than the number of pixels. This latter concern, the signal `sparsity', materially impedes the application of the method. An experimental analog using a conventional X-ray source is demonstrated and yields signal levels comparable with those expected from single biomolecules illuminated by focused XFEL pulses. The analog experiment provides an invaluable cross check on the fidelity of the reconstructed data that is not available during XFEL experiments. Using these experimental data, it is established that a sparsity of order 1.3 × 10 −3  photons per pixel per frame can be overcome, lending vital insight to the solution of the atomic resolution XFEL single-particle imaging problem by experimentally demonstrating 3D coherent diffractive imaging from photon-sparse random projections.},
doi = {10.1107/S2052252519002781},
journal = {IUCrJ},
number = 3,
volume = 6,
place = {United Kingdom},
year = {Wed Mar 20 00:00:00 EDT 2019},
month = {Wed Mar 20 00:00:00 EDT 2019}
}
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Journal Article:
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https://doi.org/10.1107/S2052252519002781
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Cited by: 19 works
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Figures / Tables:

FIG. 1 FIG. 1: Schematic of the experiment. The sample, a gold nanostructure, supported on a silicon nitride membrane, was rotated about the y-axis to obtain diffraction patterns at different orientations with respect to the optical axis, z. A first rotation series about the y-axis was followed by an in-plane rotation ofmore » the sample about z and a subsequent second rotation series. The beam attenuation and illumination time were adjusted, so that each data frame contains only about 50 scattered photons. An example of a single diffraction pattern is shown in the inset on the upper left.« less
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    Works referencing / citing this record:

    Experimental 3D Coherent Diffractive Imaging from photon-sparse random projections
    dataset, January 2019

    • Giewekemeyer, Giewekemeyer; K., K.
    • Coherent X-ray Imaging Data Bank (Lawrence Berkeley National Laboratory); European XFEL
    • DOI: 10.11577/1498641
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