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Title: Strategies for sample delivery for femtosecond crystallography

Abstract

Highly efficient data-collection methods are required for successful macromolecular crystallography (MX) experiments at X-ray free-electron lasers (XFELs). XFEL beamtime is scarce, and the high peak brightness of each XFEL pulse destroys the exposed crystal volume. It is therefore necessary to combine diffraction images from a large number of crystals (hundreds to hundreds of thousands) to obtain a final data set, bringing about sample-refreshment challenges that have previously been unknown to the MX synchrotron community. In view of this experimental complexity, a number of sample delivery methods have emerged, each with specific requirements, drawbacks and advantages. To provide useful selection criteria for future experiments, this review summarizes the currently available sample delivery methods, emphasising the basic principles and the specific sample requirements. Two main approaches to sample delivery are first covered: (i) injector methods with liquid or viscous media and (ii) fixed-target methods using large crystals or using microcrystals inside multi-crystal holders or chips. Additionally, hybrid methods such as acoustic droplet ejection and crystal extraction are covered, which combine the advantages of both fixed-target and injector approaches.

Authors:
ORCiD logo; ; ORCiD logo
Publication Date:
Sponsoring Org.:
USDOE
OSTI Identifier:
1617923
Resource Type:
Published Article
Journal Name:
Acta Crystallographica. Section D. Structural Biology
Additional Journal Information:
Journal Name: Acta Crystallographica. Section D. Structural Biology Journal Volume: 75 Journal Issue: 2; Journal ID: ISSN 2059-7983
Publisher:
International Union of Crystallography (IUCr)
Country of Publication:
United Kingdom
Language:
English

Citation Formats

Martiel, Isabelle, Müller-Werkmeister, Henrike M., and Cohen, Aina E. Strategies for sample delivery for femtosecond crystallography. United Kingdom: N. p., 2019. Web. doi:10.1107/S2059798318017953.
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Martiel, Isabelle, Müller-Werkmeister, Henrike M., & Cohen, Aina E. Strategies for sample delivery for femtosecond crystallography. United Kingdom. https://doi.org/10.1107/S2059798318017953
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Martiel, Isabelle, Müller-Werkmeister, Henrike M., and Cohen, Aina E. Tue . "Strategies for sample delivery for femtosecond crystallography". United Kingdom. https://doi.org/10.1107/S2059798318017953.
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@article{osti_1617923,
title = {Strategies for sample delivery for femtosecond crystallography},
author = {Martiel, Isabelle and Müller-Werkmeister, Henrike M. and Cohen, Aina E.},
abstractNote = {Highly efficient data-collection methods are required for successful macromolecular crystallography (MX) experiments at X-ray free-electron lasers (XFELs). XFEL beamtime is scarce, and the high peak brightness of each XFEL pulse destroys the exposed crystal volume. It is therefore necessary to combine diffraction images from a large number of crystals (hundreds to hundreds of thousands) to obtain a final data set, bringing about sample-refreshment challenges that have previously been unknown to the MX synchrotron community. In view of this experimental complexity, a number of sample delivery methods have emerged, each with specific requirements, drawbacks and advantages. To provide useful selection criteria for future experiments, this review summarizes the currently available sample delivery methods, emphasising the basic principles and the specific sample requirements. Two main approaches to sample delivery are first covered: (i) injector methods with liquid or viscous media and (ii) fixed-target methods using large crystals or using microcrystals inside multi-crystal holders or chips. Additionally, hybrid methods such as acoustic droplet ejection and crystal extraction are covered, which combine the advantages of both fixed-target and injector approaches.},
doi = {10.1107/S2059798318017953},
journal = {Acta Crystallographica. Section D. Structural Biology},
number = 2,
volume = 75,
place = {United Kingdom},
year = {Tue Feb 19 00:00:00 EST 2019},
month = {Tue Feb 19 00:00:00 EST 2019}
}
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  • DOI: 10.1038/nature13991

Crystal structure of the β2 adrenergic receptor–Gs protein complex
journal, July 2011

  • Rasmussen, Søren G. F.; DeVree, Brian T.; Zou, Yaozhong
  • Nature, Vol. 477, Issue 7366
  • DOI: 10.1038/nature10361

Concentric-flow electrokinetic injector enables serial crystallography of ribosome and photosystem II
journal, November 2015

  • Sierra, Raymond G.; Gati, Cornelius; Laksmono, Hartawan
  • Nature Methods, Vol. 13, Issue 1
  • DOI: 10.1038/nmeth.3667

‘Hit and run’ serial femtosecond crystallography of a membrane kinase in the lipid cubic phase
journal, July 2014

  • Caffrey, Martin; Li, Dianfan; Howe, Nicole
  • Philosophical Transactions of the Royal Society B: Biological Sciences, Vol. 369, Issue 1647
  • DOI: 10.1098/rstb.2013.0621

7 Å resolution in protein two-dimensional-crystal X-ray diffraction at Linac Coherent Light Source
journal, July 2014

  • Pedrini, Bill; Tsai, Ching-Ju; Capitani, Guido
  • Philosophical Transactions of the Royal Society B: Biological Sciences, Vol. 369, Issue 1647
  • DOI: 10.1098/rstb.2013.0500

Photon Beam Transport and Scientific Instruments at the European XFEL
journal, June 2017

  • Tschentscher, Thomas; Bressler, Christian; Grünert, Jan
  • Applied Sciences, Vol. 7, Issue 6
  • DOI: 10.3390/app7060592

Light-induced structural changes and the site of O=O bond formation in PSII caught by XFEL
journal, February 2017

  • Suga, Michihiro; Akita, Fusamichi; Sugahara, Michihiro
  • Nature, Vol. 543, Issue 7643
  • DOI: 10.1038/nature21400

Raster-scanning serial protein crystallography using micro- and nano-focused synchrotron beams
journal, April 2015

  • Coquelle, Nicolas; Brewster, Aaron S.; Kapp, Ulrike
  • Acta Crystallographica Section D Biological Crystallography, Vol. 71, Issue 5
  • DOI: 10.1107/S1399004715004514

Lipidic cubic phase serial millisecond crystallography using synchrotron radiation
journal, March 2015

Pink-beam serial crystallography
journal, November 2017

Batch crystallization of rhodopsin for structural dynamics using an X-ray free-electron laser
journal, June 2015

  • Wu, Wenting; Nogly, Przemyslaw; Rheinberger, Jan
  • Acta Crystallographica Section F Structural Biology Communications, Vol. 71, Issue 7
  • DOI: 10.1107/S2053230X15009966

A micro-patterned silicon chip as sample holder for macromolecular crystallography experiments with minimal background scattering
journal, May 2015

  • Roedig, P.; Vartiainen, I.; Duman, R.
  • Scientific Reports, Vol. 5, Issue 1
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The X-ray Pump–Probe instrument at the Linac Coherent Light Source
journal, April 2015

  • Chollet, Matthieu; Alonso-Mori, Roberto; Cammarata, Marco
  • Journal of Synchrotron Radiation, Vol. 22, Issue 3
  • DOI: 10.1107/S1600577515005135

Assessment of microcrystal quality by transmission electron microscopy for efficient serial femtosecond crystallography
journal, July 2016

  • Barnes, Christopher O.; Kovaleva, Elena G.; Fu, Xiaofeng
  • Archives of Biochemistry and Biophysics, Vol. 602
  • DOI: 10.1016/j.abb.2016.02.011

A three-dimensional movie of structural changes in bacteriorhodopsin
journal, December 2016

  • Nango, Eriko; Royant, Antoine; Kubo, Minoru
  • Science, Vol. 354, Issue 6319, p. 1552-1557
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Use of transmission electron microscopy to identify nanocrystals of challenging protein targets
journal, May 2014

  • Stevenson, H. P.; Makhov, A. M.; Calero, M.
  • Proceedings of the National Academy of Sciences, Vol. 111, Issue 23
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A modular and compact portable mini-endstation for high-precision, high-speed fixed target serial crystallography at FEL and synchrotron sources
journal, October 2015

  • Sherrell, Darren A.; Foster, Andrew J.; Hudson, Lee
  • Journal of Synchrotron Radiation, Vol. 22, Issue 6
  • DOI: 10.1107/S1600577515016938

Crystallography on a chip – without the chip: sheet-on-sheet sandwich
journal, October 2018

  • Doak, R. Bruce; Nass Kovacs, Gabriela; Gorel, Alexander
  • Acta Crystallographica Section D Structural Biology, Vol. 74, Issue 10
  • DOI: 10.1107/S2059798318011634

An anti-settling sample delivery instrument for serial femtosecond crystallography
journal, July 2012

  • Lomb, Lukas; Steinbrener, Jan; Bari, Sadia
  • Journal of Applied Crystallography, Vol. 45, Issue 4
  • DOI: 10.1107/S0021889812024557

Acoustic Injectors for Drop-On-Demand Serial Femtosecond Crystallography
journal, April 2016

Direct observation of ultrafast collective motions in CO myoglobin upon ligand dissociation
journal, September 2015

The New Macromolecular Femtosecond Crystallography (MFX) Instrument at LCLS
journal, January 2016

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