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Title: Synthesis of calcium carbonate nanoparticles in erythrocytes enables efficient removal of extracellular lead ions

Abstract

The “cyborg cells”, living cells with built-in nanoscaffolds, which could integrate the biological function of the cells with the functionality of nanomaterials, have been rarely explored. Here we report a method to construct “cyborg erythrocytes” through the in situ reaction of exogenous calcium and carbonate ions to generate calcium carbonate nanodots inside erythrocytes. The intracellular calcium carbonate nanodots combined with proteins are hidden under the membrane of erythrocytes, which can restrict migration and unexpected accumulation of nanodots in the body, improving the biosecurity of the nanodots. Most importantly, the calcium carbonate nanodots not only do not bring adverse effects on the nature of erythrocytes, but also endow erythrocytes with new properties. The in vitro and in vivo results show that the “cyborg erythrocytes” can remove 80% of lead ions in a blood poisoning model and reduce the lead level in the kidney and liver of mice through a precipitation transformation mechanism.

Authors:
 [1];  [1];  [1];  [1];  [1];  [1];  [2];  [2];  [2]; ORCiD logo [3]
  1. Henan Normal Univ., Henan (China). Collaborative Innovation Center of Henan Province for Green Manufacturing of Fine Chemicals
  2. Henan Normal Univ., Henan (China). Key Lab. of Green Chemical Media and Reactions
  3. Argonne National Lab. (ANL), Lemont, IL (United States)
Publication Date:
Research Org.:
Argonne National Lab. (ANL), Argonne, IL (United States)
Sponsoring Org.:
National Natural Science Foundation of China (NNSFC); USDOE Office of Energy Efficiency and Renewable Energy (EERE), Vehicle Technologies Office (EE-3V)
OSTI Identifier:
1581001
Grant/Contract Number:  
AC02-06CH11357
Resource Type:
Accepted Manuscript
Journal Name:
Communications Chemistry
Additional Journal Information:
Journal Volume: 2; Journal Issue: 1; Journal ID: ISSN 2399-3669
Publisher:
Springer Nature
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY

Citation Formats

Ru, Xiangli, Guo, Yuming, Bai, Zhengyu, Xie, Xiaoxiao, Ma, Xiaoming, Zhu, Lin, Wang, Kui, Wang, Feifei, Yang, Lin, and Lu, Jun. Synthesis of calcium carbonate nanoparticles in erythrocytes enables efficient removal of extracellular lead ions. United States: N. p., 2019. Web. https://doi.org/10.1038/s42004-019-0199-z.
Ru, Xiangli, Guo, Yuming, Bai, Zhengyu, Xie, Xiaoxiao, Ma, Xiaoming, Zhu, Lin, Wang, Kui, Wang, Feifei, Yang, Lin, & Lu, Jun. Synthesis of calcium carbonate nanoparticles in erythrocytes enables efficient removal of extracellular lead ions. United States. https://doi.org/10.1038/s42004-019-0199-z
Ru, Xiangli, Guo, Yuming, Bai, Zhengyu, Xie, Xiaoxiao, Ma, Xiaoming, Zhu, Lin, Wang, Kui, Wang, Feifei, Yang, Lin, and Lu, Jun. Fri . "Synthesis of calcium carbonate nanoparticles in erythrocytes enables efficient removal of extracellular lead ions". United States. https://doi.org/10.1038/s42004-019-0199-z. https://www.osti.gov/servlets/purl/1581001.
@article{osti_1581001,
title = {Synthesis of calcium carbonate nanoparticles in erythrocytes enables efficient removal of extracellular lead ions},
author = {Ru, Xiangli and Guo, Yuming and Bai, Zhengyu and Xie, Xiaoxiao and Ma, Xiaoming and Zhu, Lin and Wang, Kui and Wang, Feifei and Yang, Lin and Lu, Jun},
abstractNote = {The “cyborg cells”, living cells with built-in nanoscaffolds, which could integrate the biological function of the cells with the functionality of nanomaterials, have been rarely explored. Here we report a method to construct “cyborg erythrocytes” through the in situ reaction of exogenous calcium and carbonate ions to generate calcium carbonate nanodots inside erythrocytes. The intracellular calcium carbonate nanodots combined with proteins are hidden under the membrane of erythrocytes, which can restrict migration and unexpected accumulation of nanodots in the body, improving the biosecurity of the nanodots. Most importantly, the calcium carbonate nanodots not only do not bring adverse effects on the nature of erythrocytes, but also endow erythrocytes with new properties. The in vitro and in vivo results show that the “cyborg erythrocytes” can remove 80% of lead ions in a blood poisoning model and reduce the lead level in the kidney and liver of mice through a precipitation transformation mechanism.},
doi = {10.1038/s42004-019-0199-z},
journal = {Communications Chemistry},
number = 1,
volume = 2,
place = {United States},
year = {2019},
month = {9}
}

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