Synthesis of calcium carbonate nanoparticles in erythrocytes enables efficient removal of extracellular lead ions
Abstract
The “cyborg cells”, living cells with built-in nanoscaffolds, which could integrate the biological function of the cells with the functionality of nanomaterials, have been rarely explored. Here we report a method to construct “cyborg erythrocytes” through the in situ reaction of exogenous calcium and carbonate ions to generate calcium carbonate nanodots inside erythrocytes. The intracellular calcium carbonate nanodots combined with proteins are hidden under the membrane of erythrocytes, which can restrict migration and unexpected accumulation of nanodots in the body, improving the biosecurity of the nanodots. Most importantly, the calcium carbonate nanodots not only do not bring adverse effects on the nature of erythrocytes, but also endow erythrocytes with new properties. The in vitro and in vivo results show that the “cyborg erythrocytes” can remove 80% of lead ions in a blood poisoning model and reduce the lead level in the kidney and liver of mice through a precipitation transformation mechanism.
- Authors:
-
- Henan Normal Univ., Henan (China). Collaborative Innovation Center of Henan Province for Green Manufacturing of Fine Chemicals
- Henan Normal Univ., Henan (China). Key Lab. of Green Chemical Media and Reactions
- Argonne National Lab. (ANL), Lemont, IL (United States)
- Publication Date:
- Research Org.:
- Argonne National Lab. (ANL), Argonne, IL (United States)
- Sponsoring Org.:
- National Natural Science Foundation of China (NSFC); USDOE Office of Energy Efficiency and Renewable Energy (EERE), Vehicle Technologies Office (EE-3V)
- OSTI Identifier:
- 1581001
- Grant/Contract Number:
- AC02-06CH11357
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Communications Chemistry
- Additional Journal Information:
- Journal Volume: 2; Journal Issue: 1; Journal ID: ISSN 2399-3669
- Publisher:
- Springer Nature
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
Citation Formats
Ru, Xiangli, Guo, Yuming, Bai, Zhengyu, Xie, Xiaoxiao, Ma, Xiaoming, Zhu, Lin, Wang, Kui, Wang, Feifei, Yang, Lin, and Lu, Jun. Synthesis of calcium carbonate nanoparticles in erythrocytes enables efficient removal of extracellular lead ions. United States: N. p., 2019.
Web. doi:10.1038/s42004-019-0199-z.
Ru, Xiangli, Guo, Yuming, Bai, Zhengyu, Xie, Xiaoxiao, Ma, Xiaoming, Zhu, Lin, Wang, Kui, Wang, Feifei, Yang, Lin, & Lu, Jun. Synthesis of calcium carbonate nanoparticles in erythrocytes enables efficient removal of extracellular lead ions. United States. https://doi.org/10.1038/s42004-019-0199-z
Ru, Xiangli, Guo, Yuming, Bai, Zhengyu, Xie, Xiaoxiao, Ma, Xiaoming, Zhu, Lin, Wang, Kui, Wang, Feifei, Yang, Lin, and Lu, Jun. Fri .
"Synthesis of calcium carbonate nanoparticles in erythrocytes enables efficient removal of extracellular lead ions". United States. https://doi.org/10.1038/s42004-019-0199-z. https://www.osti.gov/servlets/purl/1581001.
@article{osti_1581001,
title = {Synthesis of calcium carbonate nanoparticles in erythrocytes enables efficient removal of extracellular lead ions},
author = {Ru, Xiangli and Guo, Yuming and Bai, Zhengyu and Xie, Xiaoxiao and Ma, Xiaoming and Zhu, Lin and Wang, Kui and Wang, Feifei and Yang, Lin and Lu, Jun},
abstractNote = {The “cyborg cells”, living cells with built-in nanoscaffolds, which could integrate the biological function of the cells with the functionality of nanomaterials, have been rarely explored. Here we report a method to construct “cyborg erythrocytes” through the in situ reaction of exogenous calcium and carbonate ions to generate calcium carbonate nanodots inside erythrocytes. The intracellular calcium carbonate nanodots combined with proteins are hidden under the membrane of erythrocytes, which can restrict migration and unexpected accumulation of nanodots in the body, improving the biosecurity of the nanodots. Most importantly, the calcium carbonate nanodots not only do not bring adverse effects on the nature of erythrocytes, but also endow erythrocytes with new properties. The in vitro and in vivo results show that the “cyborg erythrocytes” can remove 80% of lead ions in a blood poisoning model and reduce the lead level in the kidney and liver of mice through a precipitation transformation mechanism.},
doi = {10.1038/s42004-019-0199-z},
journal = {Communications Chemistry},
number = 1,
volume = 2,
place = {United States},
year = {Fri Sep 06 00:00:00 EDT 2019},
month = {Fri Sep 06 00:00:00 EDT 2019}
}
Web of Science
Works referenced in this record:
Magnetite biomineralization in the human brain.
journal, August 1992
- Kirschvink, J. L.; Kobayashi-Kirschvink, A.; Woodford, B. J.
- Proceedings of the National Academy of Sciences, Vol. 89, Issue 16
Aggregation-Based Crystal Growth and Microstructure Development in Natural Iron Oxyhydroxide Biomineralization Products
journal, August 2000
- Banfield, J. F.
- Science, Vol. 289, Issue 5480
Molecular tectonics in biomineralization and biomimetic materials chemistry
journal, October 1993
- Mann, Stephen
- Nature, Vol. 365, Issue 6446
Biomineralization of ferrimagnetic greigite (Fe3S4) and iron pyrite (FeS2) in a magnetotactic bacterium
journal, January 1990
- Mann, Stephen; Sparks, Nicholas H. C.; Frankel, Richard B.
- Nature, Vol. 343, Issue 6255
Cyborg cells: functionalisation of living cells with polymers and nanomaterials
journal, January 2012
- Fakhrullin, Rawil F.; Zamaleeva, Alsu I.; Minullina, Renata T.
- Chemical Society Reviews, Vol. 41, Issue 11
Polymeric Multilayer Capsules in Drug Delivery
journal, July 2010
- De Cock, Liesbeth J.; De Koker, Stefaan; De Geest, Bruno G.
- Angewandte Chemie International Edition, Vol. 49, Issue 39
Fungal Templates for Noble-Metal Nanoparticles and Their Application in Catalysis
journal, September 2008
- Bigall, Nadja C.; Reitzig, Manuela; Naumann, Wolfgang
- Angewandte Chemie International Edition, Vol. 47, Issue 41
Biogenic synthesis of silver nanoparticles and their synergistic effect with antibiotics: a study against gram-positive and gram-negative bacteria
journal, February 2010
- Fayaz, Amanulla Mohammed; Balaji, Kulandaivelu; Girilal, Morukattu
- Nanomedicine: Nanotechnology, Biology and Medicine, Vol. 6, Issue 1
Biological synthesis of metallic nanoparticles
journal, April 2010
- Thakkar, Kaushik N.; Mhatre, Snehit S.; Parikh, Rasesh Y.
- Nanomedicine: Nanotechnology, Biology and Medicine, Vol. 6, Issue 2
Biosynthesis of nanoparticles: technological concepts and future applications
journal, August 2007
- Mohanpuria, Prashant; Rana, Nisha K.; Yadav, Sudesh Kumar
- Journal of Nanoparticle Research, Vol. 10, Issue 3
Rational design of thermostable vaccines by engineered peptide-induced virus self-biomineralization under physiological conditions
journal, April 2013
- Wang, G.; Cao, R. -Y.; Chen, R.
- Proceedings of the National Academy of Sciences, Vol. 110, Issue 19
Manganese Dioxide Nanozymes as Responsive Cytoprotective Shells for Individual Living Cell Encapsulation
journal, October 2017
- Li, Wei; Liu, Zhen; Liu, Chaoqun
- Angewandte Chemie International Edition, Vol. 56, Issue 44
Silver-based crystalline nanoparticles, microbially fabricated
journal, November 1999
- Klaus, T.; Joerger, R.; Olsson, E.
- Proceedings of the National Academy of Sciences, Vol. 96, Issue 24
Scalable One-pot Bacteria-templating Synthesis Route toward Hierarchical, Porous-Co3O4 Superstructures for Supercapacitor Electrodes
journal, July 2013
- Shim, Hyun-Woo; Lim, Ah-Hyeon; Kim, Jae-Chan
- Scientific Reports, Vol. 3, Issue 1
Biosynthesis of cadmium sulphide quantum semiconductor crystallites
journal, April 1989
- Dameron, C. T.; Reese, R. N.; Mehra, R. K.
- Nature, Vol. 338, Issue 6216, p. 596-597
Bioreduction of AuCl4− Ions by the Fungus, Verticillium sp. and Surface Trapping of the Gold Nanoparticles Formed D.M. and S.S. thank the Council of Scientific and Industrial Research (CSIR), Government of India, for financial assistance.
journal, October 2001
- Mukherjee, Priyabrata; Ahmad, Absar; Mandal, Deendayal
- Angewandte Chemie International Edition, Vol. 40, Issue 19
Ordering of Quantum Dots Using Genetically Engineered Viruses
journal, May 2002
- Lee, S. -W.
- Science, Vol. 296, Issue 5569
Viral assembly of oriented quantum dot nanowires
journal, May 2003
- Mao, C.; Flynn, C. E.; Hayhurst, A.
- Proceedings of the National Academy of Sciences, Vol. 100, Issue 12
Green Synthesis of Metallic Nanoparticles via Biological Entities
journal, October 2015
- Shah, Monaliben; Fawcett, Derek; Sharma, Shashi
- Materials, Vol. 8, Issue 11
Synthesis of near-Infrared Quantum Dots in Cultured Cancer Cells
journal, December 2013
- Tan, Lianjiang; Wan, Ajun; Li, Huili
- ACS Applied Materials & Interfaces, Vol. 6, Issue 1
Microbe-mediated synthesis of antimicrobial semiconductor nanoparticles by marine bacteria
journal, March 2014
- Rajeshkumar, S.; Ponnanikajamideen, M.; Malarkodi, C.
- Journal of Nanostructure in Chemistry, Vol. 4, Issue 2
The use of microorganisms for the formation of metal nanoparticles and their application
journal, November 2005
- Mandal, Deendayal; Bolander, Mark E.; Mukhopadhyay, Debabrata
- Applied Microbiology and Biotechnology, Vol. 69, Issue 5
The unexpected effect of PEGylated gold nanoparticles on the primary function of erythrocytes
journal, January 2014
- He, Zeng; Liu, Jiaxin; Du, Libo
- Nanoscale, Vol. 6, Issue 15
Antigenically shielded universal red blood cells by polydopamine-based cell surface engineering
journal, January 2014
- Wang, Ben; Wang, Guangchuan; Zhao, Binjie
- Chem. Sci., Vol. 5, Issue 9
Engineered red blood cells as carriers for systemic delivery of a wide array of functional probes
journal, June 2014
- Shi, J.; Kundrat, L.; Pishesha, N.
- Proceedings of the National Academy of Sciences, Vol. 111, Issue 28
Multifunctional Theranostic Red Blood Cells For Magnetic-Field-Enhanced in vivo Combination Therapy of Cancer
journal, May 2014
- Wang, Chao; Sun, Xiaoqi; Cheng, Liang
- Advanced Materials, Vol. 26, Issue 28
Heavy Metal Toxicity, Part I: Arsenic and Mercury
journal, January 1998
- Graeme, Kimberlie A.; Pollack, Charles V.
- The Journal of Emergency Medicine, Vol. 16, Issue 1
Heavy metal toxicity, part ii: lead and metal fume fever
journal, March 1998
- Graeme, Kimberlie A.; Pollack, Charles V.
- The Journal of Emergency Medicine, Vol. 16, Issue 2
Chelation: Harnessing and Enhancing Heavy Metal Detoxification—A Review
journal, January 2013
- Sears, Margaret E.
- The Scientific World Journal, Vol. 2013
An Update and Review of Unconventional Metals Testing and Treatment
journal, August 2014
- Felton, Diana; Kales, Stefanos; Goldman, Rose
- Toxics, Vol. 2, Issue 3
EDTA Chelation Effects on Urinary Losses of Cadmium, Calcium, Chromium, Cobalt, Copper, Lead, Magnesium, and Zinc
journal, January 2001
- Waters, Robert S.; Bryden, Noella A.; Patterson, Kristine Y.
- Biological Trace Element Research, Vol. 83, Issue 3
Novel Oral Detoxification of Mercury, Cadmium, And Lead with Thiol-Modified Nanoporous Silica
journal, April 2014
- Sangvanich, Thanapon; Morry, Jingga; Fox, Cade
- ACS Applied Materials & Interfaces, Vol. 6, Issue 8
CuS Nanodots with Ultrahigh Efficient Renal Clearance for Positron Emission Tomography Imaging and Image-Guided Photothermal Therapy
journal, June 2015
- Zhou, Min; Li, Junjie; Liang, Su
- ACS Nano, Vol. 9, Issue 7
Control of Aragonite or Calcite Polymorphism by Mollusk Shell Macromolecules
journal, January 1996
- Falini, G.; Albeck, S.; Weiner, S.
- Science, Vol. 271, Issue 5245
Facile green synthesis of calcium carbonate/folate porous hollow spheres for the targeted pH-responsive release of anticancer drugs
journal, January 2016
- Guo, Yuming; Jia, Weili; Li, Han
- Journal of Materials Chemistry B, Vol. 4, Issue 34
Studies on the interaction between docetaxel and human hemoglobin by spectroscopic analysis and molecular docking
journal, November 2011
- Cheng, Hongxia; Liu, Hui; Bao, Wei
- Journal of Photochemistry and Photobiology B: Biology, Vol. 105, Issue 2
Prophylactic fibrinolysis through selective dissolution of nascent clots by tPA-carrying erythrocytes
journal, July 2003
- Murciano, Juan-Carlos; Medinilla, Sandra; Eslin, Donald
- Nature Biotechnology, Vol. 21, Issue 8
Clearance properties of nano-sized particles and molecules as imaging agents: considerations and caveats
journal, October 2008
- Longmire, Michelle; Choyke, Peter L.; Kobayashi, Hisataka
- Nanomedicine, Vol. 3, Issue 5
Manganese Dioxide Nanozymes as Responsive Cytoprotective Shells for Individual Living Cell Encapsulation
journal, October 2017
- Li, Wei; Liu, Zhen; Liu, Chaoqun
- Angewandte Chemie, Vol. 129, Issue 44
Fungal Templates for Noble-Metal Nanoparticles and Their Application in Catalysis
journal, September 2008
- Bigall, Nadja C.; Reitzig, Manuela; Naumann, Wolfgang
- Angewandte Chemie, Vol. 120, Issue 41
Magnetotactic Bacteria
journal, October 1982
- Blakemore, R. P.
- Annual Review of Microbiology, Vol. 36, Issue 1