In Vitro Spoilation of Silicone-Hydrogel Soft Contact Lenses in a Model-Blink Cell
Abstract
In this work, we developed an in vitro model-blink cell that reproduces the mechanism of in vivo fouling of soft contact lenses. In the model-blink cell, model tear lipid directly contacts the lens surface after forced aqueous rupture, mirroring the pre-lens tear-film breakup during interblink. Soft contact lenses are attached to a Teflon holder and immersed in artificial tear solution with protein, salts, and mucins. Artificial tear-lipid solution is spread over the air/tear interface as a duplex lipid layer. The aqueous tear film is periodically ruptured and reformed by withdrawing and reinjecting tear solution into the cell, mimicking the blink-rupture process. Fouled deposits appear on the lenses after cycling, and their compositions and spatial distributions are subsequently analyzed by optical microscopy, laser ablation electrospray ionization mass spectrometry, and two-photon fluorescence confocal scanning laser microscopy. Discrete deposit (white) spots with an average size of 20 to 300 μm are observed on the studied lenses, confirming what is seen in vivo and validating the in vitro model-blink cell. Targeted lipids (cholesterol) and proteins (albumin from bovine serum) are identified in the discrete surface deposits. Both lipid and protein occur simultaneously in the surface deposits and overlap with the white spots observed bymore »
- Authors:
-
- Univ. of California, Berkeley, CA (United States)
- Protea Biosciences Group, Morgantown, WV (United States)
- Univ. of California, Berkeley, CA (United States); Univ. of California, Berkeley, CA (United States)
- Publication Date:
- Research Org.:
- Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC)
- OSTI Identifier:
- 1571026
- Grant/Contract Number:
- AC02-05CH11231
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Optometry and Vision Science
- Additional Journal Information:
- Journal Volume: 92; Journal Issue: 7; Journal ID: ISSN 1040-5488
- Publisher:
- American Academy of Optometry
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 60 APPLIED LIFE SCIENCES; contact lens; spread lipid; protein; tear-film rupture; deposition fouling
Citation Formats
Peng, Cheng-Chun, Fajardo, Neil P., Razunguzwa, Trust, and Radke, Clayton J. In Vitro Spoilation of Silicone-Hydrogel Soft Contact Lenses in a Model-Blink Cell. United States: N. p., 2015.
Web. doi:10.1097/OPX.0000000000000625.
Peng, Cheng-Chun, Fajardo, Neil P., Razunguzwa, Trust, & Radke, Clayton J. In Vitro Spoilation of Silicone-Hydrogel Soft Contact Lenses in a Model-Blink Cell. United States. https://doi.org/10.1097/OPX.0000000000000625
Peng, Cheng-Chun, Fajardo, Neil P., Razunguzwa, Trust, and Radke, Clayton J. Wed .
"In Vitro Spoilation of Silicone-Hydrogel Soft Contact Lenses in a Model-Blink Cell". United States. https://doi.org/10.1097/OPX.0000000000000625. https://www.osti.gov/servlets/purl/1571026.
@article{osti_1571026,
title = {In Vitro Spoilation of Silicone-Hydrogel Soft Contact Lenses in a Model-Blink Cell},
author = {Peng, Cheng-Chun and Fajardo, Neil P. and Razunguzwa, Trust and Radke, Clayton J.},
abstractNote = {In this work, we developed an in vitro model-blink cell that reproduces the mechanism of in vivo fouling of soft contact lenses. In the model-blink cell, model tear lipid directly contacts the lens surface after forced aqueous rupture, mirroring the pre-lens tear-film breakup during interblink. Soft contact lenses are attached to a Teflon holder and immersed in artificial tear solution with protein, salts, and mucins. Artificial tear-lipid solution is spread over the air/tear interface as a duplex lipid layer. The aqueous tear film is periodically ruptured and reformed by withdrawing and reinjecting tear solution into the cell, mimicking the blink-rupture process. Fouled deposits appear on the lenses after cycling, and their compositions and spatial distributions are subsequently analyzed by optical microscopy, laser ablation electrospray ionization mass spectrometry, and two-photon fluorescence confocal scanning laser microscopy. Discrete deposit (white) spots with an average size of 20 to 300 μm are observed on the studied lenses, confirming what is seen in vivo and validating the in vitro model-blink cell. Targeted lipids (cholesterol) and proteins (albumin from bovine serum) are identified in the discrete surface deposits. Both lipid and protein occur simultaneously in the surface deposits and overlap with the white spots observed by optical microscopy. Additionally, lipid and protein penetrate into the bulk of tested silicone-hydrogel lenses, likely attributed to the bicontinuous microstructure of oleophilic silicone and hydrophilic polymer phases of the lens. In vitro spoilation of soft contact lenses is successfully achieved by the model-blink cell confirming the tear rupture/deposition mechanism of lens fouling. The model-blink cell provides a reliable laboratory tool for screening new antifouling lens materials, surface coatings, and care solutions.},
doi = {10.1097/OPX.0000000000000625},
journal = {Optometry and Vision Science},
number = 7,
volume = 92,
place = {United States},
year = {Wed Jul 01 00:00:00 EDT 2015},
month = {Wed Jul 01 00:00:00 EDT 2015}
}
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