T cell activation and immune synapse organization respond to the microscale mechanics of structured surfaces
Abstract
Cells have the remarkable ability to sense the mechanical stiffness of their surroundings. This has been studied extensively in the context of cells interacting with planar surfaces, a conceptually elegant model that also has application in biomaterial design. However, physiological interfaces are spatially complex, exhibiting topographical features that are described over multiple scales. This report explores mechanosensing of microstructured elastomer surfaces by CD4 + T cells, key mediators of the adaptive immune response. We show that T cells form complex interactions with elastomer micropillar arrays, extending processes into spaces between structures and forming local areas of contraction and expansion dictated by the layout of microtubules within this interface. Conversely, cytoskeletal reorganization and intracellular signaling are sensitive to the pillar dimensions and flexibility. Unexpectedly, these measures show different responses to substrate rigidity, suggesting competing processes in overall T cell mechanosensing. The results of this study demonstrate that T cells sense the local rigidity of their environment, leading to strategies for biomaterial design.
- Authors:
- Publication Date:
- Research Org.:
- Brookhaven National Laboratory (BNL), Upton, NY (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1562313
- Alternate Identifier(s):
- OSTI ID: 1606184
- Report Number(s):
- BNL-213752-2020-JAAM
Journal ID: ISSN 0027-8424
- Grant/Contract Number:
- SC0012704
- Resource Type:
- Published Article
- Journal Name:
- Proceedings of the National Academy of Sciences of the United States of America
- Additional Journal Information:
- Journal Name: Proceedings of the National Academy of Sciences of the United States of America Journal Volume: 116 Journal Issue: 40; Journal ID: ISSN 0027-8424
- Publisher:
- National Academy of Sciences
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 77 NANOSCIENCE AND NANOTECHNOLOGY; 59 BASIC BIOLOGICAL SCIENCES; mechanobiology; T cell; microstructure
Citation Formats
Jin, Weiyang, Tamzalit, Fella, Chaudhuri, Parthiv Kant, Black, Charles T., Huse, Morgan, and Kam, Lance C. T cell activation and immune synapse organization respond to the microscale mechanics of structured surfaces. United States: N. p., 2019.
Web. doi:10.1073/pnas.1906986116.
Jin, Weiyang, Tamzalit, Fella, Chaudhuri, Parthiv Kant, Black, Charles T., Huse, Morgan, & Kam, Lance C. T cell activation and immune synapse organization respond to the microscale mechanics of structured surfaces. United States. https://doi.org/10.1073/pnas.1906986116
Jin, Weiyang, Tamzalit, Fella, Chaudhuri, Parthiv Kant, Black, Charles T., Huse, Morgan, and Kam, Lance C. Mon .
"T cell activation and immune synapse organization respond to the microscale mechanics of structured surfaces". United States. https://doi.org/10.1073/pnas.1906986116.
@article{osti_1562313,
title = {T cell activation and immune synapse organization respond to the microscale mechanics of structured surfaces},
author = {Jin, Weiyang and Tamzalit, Fella and Chaudhuri, Parthiv Kant and Black, Charles T. and Huse, Morgan and Kam, Lance C.},
abstractNote = {Cells have the remarkable ability to sense the mechanical stiffness of their surroundings. This has been studied extensively in the context of cells interacting with planar surfaces, a conceptually elegant model that also has application in biomaterial design. However, physiological interfaces are spatially complex, exhibiting topographical features that are described over multiple scales. This report explores mechanosensing of microstructured elastomer surfaces by CD4 + T cells, key mediators of the adaptive immune response. We show that T cells form complex interactions with elastomer micropillar arrays, extending processes into spaces between structures and forming local areas of contraction and expansion dictated by the layout of microtubules within this interface. Conversely, cytoskeletal reorganization and intracellular signaling are sensitive to the pillar dimensions and flexibility. Unexpectedly, these measures show different responses to substrate rigidity, suggesting competing processes in overall T cell mechanosensing. The results of this study demonstrate that T cells sense the local rigidity of their environment, leading to strategies for biomaterial design.},
doi = {10.1073/pnas.1906986116},
journal = {Proceedings of the National Academy of Sciences of the United States of America},
number = 40,
volume = 116,
place = {United States},
year = {Mon Sep 16 00:00:00 EDT 2019},
month = {Mon Sep 16 00:00:00 EDT 2019}
}
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https://doi.org/10.1073/pnas.1906986116
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