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Title: From powerhouse to processing plant: conserved roles of mitochondrial outer membrane proteins in tRNA splicing

Abstract

The mitochondrial cytoplasmic surface serves as a processing site for numerous RNAs from budding yeast to metazoans. We report that budding yeast mitochondrial outer membrane (MOM) proteins that are subunits of the translocase of the outer mitochondrial membrane (Tom70 and Tom 22) and sorting and assembly machinery (Sam37) are required for efficient pretransfer RNA (pre-tRNA) splicing. Defective pre-tRNA splicing in MOM mutants is due not to loss of respiratory metabolism but instead inefficient targeting/tethering of tRNA splicing endonuclease (SEN) subunits to mitochondria. Schizosaccharomyces pombe SEN subunits also localize to mitochondria, and Tom70 is required for this localization and pre-tRNA splicing. Thus, the role of MOM protein in targeting/tethering SEN subunits to mitochondria has been conserved for >500 million years.

Authors:
 [1];  [1]
  1. The Ohio State Univ., Columbus, OH (United States)
Publication Date:
Research Org.:
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Sponsoring Org.:
USDOE Office of Science (SC)
OSTI Identifier:
1560569
Grant/Contract Number:  
AC02-05CH11231
Resource Type:
Accepted Manuscript
Journal Name:
Genes & Development
Additional Journal Information:
Journal Volume: 32; Journal Issue: 19-20; Journal ID: ISSN 0890-9369
Publisher:
Cold Springs Harbor Press
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Wan, Yao, and Hopper, Anita K. From powerhouse to processing plant: conserved roles of mitochondrial outer membrane proteins in tRNA splicing. United States: N. p., 2018. Web. doi:10.1101/gad.316257.118.
Wan, Yao, & Hopper, Anita K. From powerhouse to processing plant: conserved roles of mitochondrial outer membrane proteins in tRNA splicing. United States. doi:10.1101/gad.316257.118.
Wan, Yao, and Hopper, Anita K. Tue . "From powerhouse to processing plant: conserved roles of mitochondrial outer membrane proteins in tRNA splicing". United States. doi:10.1101/gad.316257.118. https://www.osti.gov/servlets/purl/1560569.
@article{osti_1560569,
title = {From powerhouse to processing plant: conserved roles of mitochondrial outer membrane proteins in tRNA splicing},
author = {Wan, Yao and Hopper, Anita K.},
abstractNote = {The mitochondrial cytoplasmic surface serves as a processing site for numerous RNAs from budding yeast to metazoans. We report that budding yeast mitochondrial outer membrane (MOM) proteins that are subunits of the translocase of the outer mitochondrial membrane (Tom70 and Tom 22) and sorting and assembly machinery (Sam37) are required for efficient pretransfer RNA (pre-tRNA) splicing. Defective pre-tRNA splicing in MOM mutants is due not to loss of respiratory metabolism but instead inefficient targeting/tethering of tRNA splicing endonuclease (SEN) subunits to mitochondria. Schizosaccharomyces pombe SEN subunits also localize to mitochondria, and Tom70 is required for this localization and pre-tRNA splicing. Thus, the role of MOM protein in targeting/tethering SEN subunits to mitochondria has been conserved for >500 million years.},
doi = {10.1101/gad.316257.118},
journal = {Genes & Development},
number = 19-20,
volume = 32,
place = {United States},
year = {2018},
month = {9}
}

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Figures / Tables:

Figure 1 Figure 1: Respiratory metabolism is not required for efficient pretRNA splicing in S. cerevisiae. (A) Northern analysis of tRNAIleUAU (probe JW0041) in cells lacking mitochondrial DNA. Wild-type cells lacking mitochondrial DNA via treatment with ethidium bromide [wild-type (EtBr)] and mip cells. (P) Primary tRNA transcript; (I) end-processed intron-containing tRNA; (5S)more » 5S rRNA stained with EtBr; (I/P) signal intensity of end-processed intron-containing tRNA/primary tRNA transcript, normalized to wild type. (B) Statistical analysis of end-processed intron-containing tRNA/primary tRNA transcript ratios compared with wild type from three biological repeats. (ns) P > 0.05; (∗∗) P≤ 0.01; (∗∗∗) P≤0.001; (∗∗∗∗) P≤0.0001.« less

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