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Title: Promoting microbial utilization of phenolic substrates from bio-oil

Abstract

The economic viability of the biorefinery concept is limited by the valorization of lignin. One possible method of lignin valorization is biological upgrading with aromatic-catabolic microbes. In conjunction, lignin monomers can be produced by fast pyrolysis and fractionation. However, biological upgrading of these lignin monomers is limited by low water solubility. Here, we address the problem of low water solubility with an emulsifier blend containing approximately 70 wt% Tween® 20 and 30 wt% Span® 80. Pseudomonas putida KT2440 grew to an optical density (OD600) of 1.0 ± 0.2 when supplied with 1.6 wt% emulsified phenolic monomer-rich product produced by fast pyrolysis of red oak using an emulsifier dose of 0.076 ± 0.002g emulsifier blend per g of phenolic monomer-rich product. This approach partially mitigated the toxicity of the model phenolic monomer p-coumarate to the microbe, but not benzoate or vanillin. This study provides a proof of concept that processing of biomass-derived phenolics to increase aqueous availability can enhance microbial utilization.

Authors:
 [1];  [2];  [3];  [2];  [3];  [4];  [2];  [1]
+ Show Author Affiliations
  1. grid.34421.30 0000 0004 1936 7312 Chemical and Biological Engineering, 4134 Biorenewable Research Laboratory Iowa State University 50011 Ames IA USA
  2. grid.34421.30 0000 0004 1936 7312 Bioeconomy Institute Iowa State University 1140 BRL Building, 617 Bissell Road 50011 Ames IA USA
  3. grid.419357.d 0000 0001 2199 3636 National Bioenergy Center National Renewable Energy Laboratory 80401 Golden CO USA
  4. grid.34421.30 0000 0004 1936 7312 Department of Food Science and Human Nutrition Iowa State University 50011 Ames IA USA
Publication Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE)
OSTI Identifier:
1772502
Alternate Identifier(s):
OSTI ID: 1543118
Report Number(s):
NREL/JA-5100-74319
Journal ID: ISSN 1367-5435
Grant/Contract Number:  
AC36-08GO28308
Resource Type:
Published Article
Journal Name:
Journal of Industrial Microbiology and Biotechnology
Additional Journal Information:
Journal Name: Journal of Industrial Microbiology and Biotechnology Journal Volume: 46 Journal Issue: 11; Journal ID: ISSN 1367-5435
Publisher:
Oxford University Press
Country of Publication:
Germany
Language:
English
Subject:
09 BIOMASS FUELS; Pseudomonas putida KT2440; lignin; bio-oil; emulsion; phenols

Citation Formats

Davis, Kirsten, Rover, Marjorie R., Salvachúa, Davinia, Smith, Ryan G., Beckham, Gregg T., Wen, Zhiyou, Brown, Robert C., and Jarboe, Laura R. Promoting microbial utilization of phenolic substrates from bio-oil. Germany: N. p., 2019. Web. doi:10.1007/s10295-019-02208-z.
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Davis, Kirsten, Rover, Marjorie R., Salvachúa, Davinia, Smith, Ryan G., Beckham, Gregg T., Wen, Zhiyou, Brown, Robert C., & Jarboe, Laura R. Promoting microbial utilization of phenolic substrates from bio-oil. Germany. https://doi.org/10.1007/s10295-019-02208-z
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Davis, Kirsten, Rover, Marjorie R., Salvachúa, Davinia, Smith, Ryan G., Beckham, Gregg T., Wen, Zhiyou, Brown, Robert C., and Jarboe, Laura R. Fri . "Promoting microbial utilization of phenolic substrates from bio-oil". Germany. https://doi.org/10.1007/s10295-019-02208-z.
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@article{osti_1772502,
title = {Promoting microbial utilization of phenolic substrates from bio-oil},
author = {Davis, Kirsten and Rover, Marjorie R. and Salvachúa, Davinia and Smith, Ryan G. and Beckham, Gregg T. and Wen, Zhiyou and Brown, Robert C. and Jarboe, Laura R.},
abstractNote = {The economic viability of the biorefinery concept is limited by the valorization of lignin. One possible method of lignin valorization is biological upgrading with aromatic-catabolic microbes. In conjunction, lignin monomers can be produced by fast pyrolysis and fractionation. However, biological upgrading of these lignin monomers is limited by low water solubility. Here, we address the problem of low water solubility with an emulsifier blend containing approximately 70 wt% Tween® 20 and 30 wt% Span® 80. Pseudomonas putida KT2440 grew to an optical density (OD600) of 1.0 ± 0.2 when supplied with 1.6 wt% emulsified phenolic monomer-rich product produced by fast pyrolysis of red oak using an emulsifier dose of 0.076 ± 0.002g emulsifier blend per g of phenolic monomer-rich product. This approach partially mitigated the toxicity of the model phenolic monomer p-coumarate to the microbe, but not benzoate or vanillin. This study provides a proof of concept that processing of biomass-derived phenolics to increase aqueous availability can enhance microbial utilization.},
doi = {10.1007/s10295-019-02208-z},
journal = {Journal of Industrial Microbiology and Biotechnology},
number = 11,
volume = 46,
place = {Germany},
year = {Fri Nov 01 00:00:00 EDT 2019},
month = {Fri Nov 01 00:00:00 EDT 2019}
}
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Journal Article:
Free Publicly Available Full Text
Publisher's Version of Record
https://doi.org/10.1007/s10295-019-02208-z
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Cited by: 12 works
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Figures / Tables:

Fig. 1 Fig. 1: Schematic of the fast pyrolysis reactor and the heavy and light ends of bio-oil recovery. The heavy ends are subjected to liquid–liquid extraction using water as the solvent during bio-oil production. The products from the liquid–liquid extraction are unrefined sugars and water-insoluble phenolic oil. The cyclones collect themore » bio-char while the light ends contain the water and is rich in acetate. The heavy-end water-insoluble phenolics are subjected to liquid–liquid extraction utilizing toluene as the solvent. Following removal of the toluene, the phenolic monomer-rich product is subjected to microbial utilization« less
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    Table 1: Part 3 (p. 15)table
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