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Title: Promoting microbial utilization of phenolic substrates from bio-oil

Abstract

The economic viability of the biorefinery concept is limited by the valorization of lignin. One possible method of lignin valorization is biological upgrading with aromatic-catabolic microbes. In conjunction, lignin monomers can be produced by fast pyrolysis and fractionation. However, biological upgrading of these lignin monomers is limited by low water solubility. Here, we address the problem of low water solubility with an emulsifier blend containing approximately 70 wt% Tween ® 20 and 30 wt% Span ® 80. Pseudomonas putida KT2440 grew to an optical density (OD 600) of 1.0 ± 0.2 when supplied with 1.6 wt% emulsified phenolic monomer-rich product produced by fast pyrolysis of red oak using an emulsifier dose of 0.076 ± 0.002g emulsifier blend per g of phenolic monomer-rich product. This approach partially mitigated the toxicity of the model phenolic monomer p-coumarate to the microbe, but not benzoate or vanillin. This study provides a proof of concept that processing of biomass-derived phenolics to increase aqueous availability can enhance microbial utilization.

Authors:
 [1];  [2]; ORCiD logo [3];  [2]; ORCiD logo [3];  [4];  [2];  [1]
  1. Iowa State Univ., Ames, IA (United States). Chemical and Biological Engineering
  2. Iowa State Univ., Ames, IA (United States). Bioeconomy Inst.
  3. National Renewable Energy Laboratory (NREL), Golden, CO (United States)
  4. Iowa State Univ., Ames, IA (United States). Dept. of Food Science and Human Nutrition
Publication Date:
Research Org.:
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE)
OSTI Identifier:
1543118
Report Number(s):
NREL/JA-5100-74319
Journal ID: ISSN 1367-5435
Grant/Contract Number:  
AC36-08GO28308
Resource Type:
Accepted Manuscript
Journal Name:
Journal of Industrial Microbiology and Biotechnology
Additional Journal Information:
Journal Volume: none; Journal Issue: none; Journal ID: ISSN 1367-5435
Publisher:
Springer
Country of Publication:
United States
Language:
English
Subject:
09 BIOMASS FUELS; Pseudomonas putida KT2440; lignin; bio-oil; emulsion; phenols

Citation Formats

Davis, Kirsten, Rover, Marjorie R., Salvachua Rodriguez, Davinia, Smith, Ryan G., Beckham, Gregg T, Wen, Zhiyou, Brown, Robert C., and Jarboe, Laura R. Promoting microbial utilization of phenolic substrates from bio-oil. United States: N. p., 2019. Web. doi:10.1007/s10295-019-02208-z.
Davis, Kirsten, Rover, Marjorie R., Salvachua Rodriguez, Davinia, Smith, Ryan G., Beckham, Gregg T, Wen, Zhiyou, Brown, Robert C., & Jarboe, Laura R. Promoting microbial utilization of phenolic substrates from bio-oil. United States. doi:10.1007/s10295-019-02208-z.
Davis, Kirsten, Rover, Marjorie R., Salvachua Rodriguez, Davinia, Smith, Ryan G., Beckham, Gregg T, Wen, Zhiyou, Brown, Robert C., and Jarboe, Laura R. Thu . "Promoting microbial utilization of phenolic substrates from bio-oil". United States. doi:10.1007/s10295-019-02208-z.
@article{osti_1543118,
title = {Promoting microbial utilization of phenolic substrates from bio-oil},
author = {Davis, Kirsten and Rover, Marjorie R. and Salvachua Rodriguez, Davinia and Smith, Ryan G. and Beckham, Gregg T and Wen, Zhiyou and Brown, Robert C. and Jarboe, Laura R.},
abstractNote = {The economic viability of the biorefinery concept is limited by the valorization of lignin. One possible method of lignin valorization is biological upgrading with aromatic-catabolic microbes. In conjunction, lignin monomers can be produced by fast pyrolysis and fractionation. However, biological upgrading of these lignin monomers is limited by low water solubility. Here, we address the problem of low water solubility with an emulsifier blend containing approximately 70 wt% Tween® 20 and 30 wt% Span® 80. Pseudomonas putida KT2440 grew to an optical density (OD600) of 1.0 ± 0.2 when supplied with 1.6 wt% emulsified phenolic monomer-rich product produced by fast pyrolysis of red oak using an emulsifier dose of 0.076 ± 0.002g emulsifier blend per g of phenolic monomer-rich product. This approach partially mitigated the toxicity of the model phenolic monomer p-coumarate to the microbe, but not benzoate or vanillin. This study provides a proof of concept that processing of biomass-derived phenolics to increase aqueous availability can enhance microbial utilization.},
doi = {10.1007/s10295-019-02208-z},
journal = {Journal of Industrial Microbiology and Biotechnology},
number = none,
volume = none,
place = {United States},
year = {2019},
month = {7}
}

Journal Article:
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Works referenced in this record:

Genomic analysis of the aromatic catabolic pathways from Pseudomonas putida KT2440
journal, December 2002


Production of Clean Pyrolytic Sugars for Fermentation
journal, April 2014

  • Rover, Marjorie R.; Johnston, Patrick A.; Jin, Tao
  • ChemSusChem, Vol. 7, Issue 6, p. 1662-1668
  • DOI: 10.1002/cssc.201301259

Lignin valorization through integrated biological funneling and chemical catalysis
journal, August 2014

  • Linger, J. G.; Vardon, D. R.; Guarnieri, M. T.
  • Proceedings of the National Academy of Sciences, Vol. 111, Issue 33, p. 12013-12018
  • DOI: 10.1073/pnas.1410657111

Effect of selected aldehydes on the growth and fermentation of ethanologenic Escherichia coli
journal, October 1999


    Works referencing / citing this record:

    Lignin valorization through integrated biological funneling and chemical catalysis
    journal, August 2014

    • Linger, J. G.; Vardon, D. R.; Guarnieri, M. T.
    • Proceedings of the National Academy of Sciences, Vol. 111, Issue 33, p. 12013-12018
    • DOI: 10.1073/pnas.1410657111

    Genomic analysis of the aromatic catabolic pathways from Pseudomonas putida KT2440
    journal, December 2002


    Production of Clean Pyrolytic Sugars for Fermentation
    journal, April 2014

    • Rover, Marjorie R.; Johnston, Patrick A.; Jin, Tao
    • ChemSusChem, Vol. 7, Issue 6, p. 1662-1668
    • DOI: 10.1002/cssc.201301259