Base-Catalyzed Depolymerization of Solid Lignin-Rich Streams Enables Microbial Conversion
Abstract
Lignin valorization offers significant potential to enhance the economic viability of lignocellulosic biorefineries. However, because of its heterogeneous and recalcitrant nature, conversion of lignin to value-added coproducts remains a considerable technical challenge. In this study, we employ base-catalyzed depolymerization (BCD) using a process-relevant solid lignin stream produced via deacetylation, mechanical refining, and enzymatic hydrolysis to enable biological lignin conversion. BCD was conducted with the solid lignin substrate over a range of temperatures at two NaOH concentrations, and the results demonstrate that the lignin can be partially extracted and saponified at temperatures as low as 60 °C. At 120 °C and 2% NaOH, the high extent of lignin solubility was accompanied by a considerable decrease in the lignin average molecular weight and the release of lignin-derived monomers including hydroxycinnamic acids. BCD liquors were tested for microbial growth using seven aromatic-catabolizing bacteria and two yeasts. Three organisms (Pseudomonas putida KT2440, Rhodotorula mucilaginosa, and Corynebacterium glutamicum) tolerate high BCD liquor concentrations (up to 90% v/v) and rapidly consume the main lignin-derived monomers, resulting in lignin conversion of up to 15%. Furthermore, as a proof of concept, muconic acid production from a representative lignin BCD liquor was demonstrated with an engineered P. putida KT2440more »
- Authors:
-
[1];
[2];
[2];
- Sandia National Lab. (SNL-CA), Livermore, CA (United States). Biomass Science and Conversion Technology Dept.
- National Renewable Energy Lab. (NREL), Golden, CO (United States). National Bioenergy Center
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Biological Systems and Engineering Division; USDOE Joint BioEnergy Inst., Emeryville, CA (United States)
- Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Biological Systems and Engineering Division; USDOE Joint BioEnergy Inst., Emeryville, CA (United States); Univ. of California, Berkeley, CA (United States). Dept. of Chemical and Biomolecular Engineering and Bioengineering; Novo Nordisk Foundation Center for Sustainability, Lyngby (Denmark)
- Sandia National Lab. (SNL-CA), Livermore, CA (United States). Biomass Science and Conversion Technology Dept.; USDOE Joint BioEnergy Inst., Emeryville, CA (United States); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Biological Systems and Engineering Division
- Sandia National Lab. (SNL-CA), Livermore, CA (United States). Biomass Science and Conversion Technology Dept.; USDOE Joint BioEnergy Inst., Emeryville, CA (United States)
- Publication Date:
- Research Org.:
- National Renewable Energy Laboratory (NREL), Golden, CO (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
- Sponsoring Org.:
- USDOE Office of Energy Efficiency and Renewable Energy (EERE), Sustainable Transportation Office. Bioenergy Technologies Office; USDOE Office of Science (SC), Biological and Environmental Research (BER)
- OSTI Identifier:
- 1394906
- Alternate Identifier(s):
- OSTI ID: 1476536
- Report Number(s):
- NREL/JA-5100-70189
Journal ID: ISSN 2168-0485
- Grant/Contract Number:
- AC36-08GO28308; AC02-05CH11231; NA0003525
- Resource Type:
- Accepted Manuscript
- Journal Name:
- ACS Sustainable Chemistry & Engineering
- Additional Journal Information:
- Journal Volume: 5; Journal Issue: 9; Journal ID: ISSN 2168-0485
- Publisher:
- American Chemical Society (ACS)
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 09 BIOMASS FUELS; 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; base-catalyzed depolymerization; biological conversion; lignin liquor; lignin monomers; muconic acid
Citation Formats
Rodriguez, Alberto, Salvachúa, Davinia, Katahira, Rui, Black, Brenna A., Cleveland, Nicholas S., Reed, Michelle, Smith, Holly, Baidoo, Edward E. K., Keasling, Jay D., Simmons, Blake A., Beckham, Gregg T., and Gladden, John M.. Base-Catalyzed Depolymerization of Solid Lignin-Rich Streams Enables Microbial Conversion. United States: N. p., 2017.
Web. doi:10.1021/acssuschemeng.7b01818.
Rodriguez, Alberto, Salvachúa, Davinia, Katahira, Rui, Black, Brenna A., Cleveland, Nicholas S., Reed, Michelle, Smith, Holly, Baidoo, Edward E. K., Keasling, Jay D., Simmons, Blake A., Beckham, Gregg T., & Gladden, John M.. Base-Catalyzed Depolymerization of Solid Lignin-Rich Streams Enables Microbial Conversion. United States. https://doi.org/10.1021/acssuschemeng.7b01818
Rodriguez, Alberto, Salvachúa, Davinia, Katahira, Rui, Black, Brenna A., Cleveland, Nicholas S., Reed, Michelle, Smith, Holly, Baidoo, Edward E. K., Keasling, Jay D., Simmons, Blake A., Beckham, Gregg T., and Gladden, John M.. Tue .
"Base-Catalyzed Depolymerization of Solid Lignin-Rich Streams Enables Microbial Conversion". United States. https://doi.org/10.1021/acssuschemeng.7b01818. https://www.osti.gov/servlets/purl/1394906.
@article{osti_1394906,
title = {Base-Catalyzed Depolymerization of Solid Lignin-Rich Streams Enables Microbial Conversion},
author = {Rodriguez, Alberto and Salvachúa, Davinia and Katahira, Rui and Black, Brenna A. and Cleveland, Nicholas S. and Reed, Michelle and Smith, Holly and Baidoo, Edward E. K. and Keasling, Jay D. and Simmons, Blake A. and Beckham, Gregg T. and Gladden, John M.},
abstractNote = {Lignin valorization offers significant potential to enhance the economic viability of lignocellulosic biorefineries. However, because of its heterogeneous and recalcitrant nature, conversion of lignin to value-added coproducts remains a considerable technical challenge. In this study, we employ base-catalyzed depolymerization (BCD) using a process-relevant solid lignin stream produced via deacetylation, mechanical refining, and enzymatic hydrolysis to enable biological lignin conversion. BCD was conducted with the solid lignin substrate over a range of temperatures at two NaOH concentrations, and the results demonstrate that the lignin can be partially extracted and saponified at temperatures as low as 60 °C. At 120 °C and 2% NaOH, the high extent of lignin solubility was accompanied by a considerable decrease in the lignin average molecular weight and the release of lignin-derived monomers including hydroxycinnamic acids. BCD liquors were tested for microbial growth using seven aromatic-catabolizing bacteria and two yeasts. Three organisms (Pseudomonas putida KT2440, Rhodotorula mucilaginosa, and Corynebacterium glutamicum) tolerate high BCD liquor concentrations (up to 90% v/v) and rapidly consume the main lignin-derived monomers, resulting in lignin conversion of up to 15%. Furthermore, as a proof of concept, muconic acid production from a representative lignin BCD liquor was demonstrated with an engineered P. putida KT2440 strain. These results highlight the potential for a mild lignin depolymerization process to enhance the microbial conversion of solid lignin-rich biorefinery streams.},
doi = {10.1021/acssuschemeng.7b01818},
journal = {ACS Sustainable Chemistry & Engineering},
number = 9,
volume = 5,
place = {United States},
year = {2017},
month = {8}
}
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Works referenced in this record:
Aromatic catabolic pathway selection for optimal production of pyruvate and lactate from lignin
journal, March 2015
- Johnson, Christopher W.; Beckham, Gregg T.
- Metabolic Engineering, Vol. 28
Towards lignin consolidated bioprocessing: simultaneous lignin depolymerization and product generation by bacteria
journal, January 2015
- Salvachúa, Davinia; Karp, Eric M.; Nimlos, Claire T.
- Green Chemistry, Vol. 17, Issue 11
Inhibition of ethanol-producing yeast and bacteria by degradation products produced during pre-treatment of biomass
journal, August 2004
- Klinke, H. B.; Thomsen, A. B.; Ahring, B. K.
- Applied Microbiology and Biotechnology, Vol. 66, Issue 1, p. 10-26
Combining Metabolic Engineering and Electrocatalysis: Application to the Production of Polyamides from Sugar
journal, January 2016
- Suastegui, Miguel; Matthiesen, John E.; Carraher, Jack M.
- Angewandte Chemie International Edition, Vol. 55, Issue 7
Biological valorization of low molecular weight lignin
journal, December 2016
- Abdelaziz, Omar Y.; Brink, Daniel P.; Prothmann, Jens
- Biotechnology Advances, Vol. 34, Issue 8
Alkaline Pretreatment of Corn Stover: Bench-Scale Fractionation and Stream Characterization
journal, May 2014
- Karp, Eric M.; Donohoe, Bryon S.; O’Brien, Marykate H.
- ACS Sustainable Chemistry & Engineering, Vol. 2, Issue 6, p. 1481-1491
Conversion of lignin to aromatic-based chemicals (L-chems) and biofuels (L-fuels)
journal, October 2012
- Beauchet, R.; Monteil-Rivera, F.; Lavoie, J. M.
- Bioresource Technology, Vol. 121
Catalytic Transformation of Lignin for the Production of Chemicals and Fuels
journal, October 2015
- Li, Changzhi; Zhao, Xiaochen; Wang, Aiqin
- Chemical Reviews, Vol. 115, Issue 21, p. 11559-11624
Cultivation of oleaginous Rhodotorula mucilaginosa in airlift bioreactor by using seawater
journal, February 2016
- Yen, Hong-Wei; Liao, Yu-Ting; Liu, Yi Xian
- Journal of Bioscience and Bioengineering, Vol. 121, Issue 2
cis,cis-Muconic acid: separation and catalysis to bio-adipic acid for nylon-6,6 polymerization
journal, January 2016
- Vardon, Derek R.; Rorrer, Nicholas A.; Salvachúa, Davinia
- Green Chemistry, Vol. 18, Issue 11
From dirt to industrial applications: Pseudomonas putida as a Synthetic Biology chassis for hosting harsh biochemical reactions
journal, October 2016
- Nikel, Pablo I.; Chavarría, Max; Danchin, Antoine
- Current Opinion in Chemical Biology, Vol. 34
Biomass-derived monomers for performance-differentiated fiber reinforced polymer composites
journal, January 2017
- Rorrer, Nicholas A.; Vardon, Derek R.; Dorgan, John R.
- Green Chemistry, Vol. 19, Issue 12
Lignin valorization through integrated biological funneling and chemical catalysis
journal, August 2014
- Linger, J. G.; Vardon, D. R.; Guarnieri, M. T.
- Proceedings of the National Academy of Sciences, Vol. 111, Issue 33, p. 12013-12018
Degradation and assimilation of aromatic compounds by Corynebacterium glutamicum: another potential for applications for this bacterium?
journal, May 2012
- Shen, Xi-Hui; Zhou, Ning-Yi; Liu, Shuang-Jiang
- Applied Microbiology and Biotechnology, Vol. 95, Issue 1
Systems biology-guided biodesign of consolidated lignin conversion
journal, January 2016
- Lin, Lu; Cheng, Yanbing; Pu, Yunqiao
- Green Chemistry, Vol. 18, Issue 20
Lignin depolymerization by fungal secretomes and a microbial sink
journal, January 2016
- Salvachúa, Davinia; Katahira, Rui; Cleveland, Nicholas S.
- Green Chemistry, Vol. 18, Issue 22
Comparison of alkali treatments for efficient release of p-coumaric acid and enzymatic saccharification of sorghum pith
journal, May 2016
- Jiang, Kankan; Li, Lulu; Long, Liangkun
- Bioresource Technology, Vol. 207
The β-Ketoadipate Pathway And The Biology Of Self-Identity
journal, October 1996
- Harwood, Caroline S.; Parales, Rebecca E.
- Annual Review of Microbiology, Vol. 50, Issue 1, p. 553-590
Genetic and Biochemical Investigations on Bacterial Catabolic Pathways for Lignin-Derived Aromatic Compounds
journal, January 2007
- Masai, Eiji; Katayama, Yoshihiro; Fukuda, Masao
- Bioscience, Biotechnology, and Biochemistry, Vol. 71, Issue 1
Rapid base-catalyzed decarboxylation and amide-forming reaction of substituted cinnamic acids via microwave heating
journal, January 2005
- Nomura, Eisaku; Hosoda, Asao; Mori, Hajime
- Green Chemistry, Vol. 7, Issue 12
Grass lignin acylation: p-coumaroyl transferase activity and cell wall characteristics of C3 and C4 grasses
journal, March 2009
- Hatfield, Ronald D.; Marita, Jane M.; Frost, Kenneth
- Planta, Vol. 229, Issue 6, p. 1253-1267
Lignin Biosynthesis and Structure
journal, May 2010
- Vanholme, R.; Demedts, B.; Morreel, K.
- Plant Physiology, Vol. 153, Issue 3, p. 895-905
Structural Characterization of Wheat Straw Lignin as Revealed by Analytical Pyrolysis, 2D-NMR, and Reductive Cleavage Methods
journal, December 2011
- del Río, José C.; Rencoret, Jorge; Prinsen, Pepijn
- Journal of Agricultural and Food Chemistry, Vol. 60, Issue 23
Structural Redesigning Arabidopsis Lignins into Alkali-Soluble Lignins through the Expression of p- Coumaroyl-CoA:Monolignol Transferase PMT
journal, January 2016
- Sibout, Richard; Le Bris, Philippe; Legée, Frédéric
- Plant Physiology, Vol. 170, Issue 3
Genomic analysis of the aromatic catabolic pathways from Pseudomonas putida KT2440
journal, December 2002
- Jimenez, Jose Ignacio; Minambres, Baltasar; Garcia, Jose Luis
- Environmental Microbiology, Vol. 4, Issue 12, p. 824-841
Tricin-lignins: occurrence and quantitation of tricin in relation to phylogeny
journal, November 2016
- Lan, Wu; Rencoret, Jorge; Lu, Fachuang
- The Plant Journal, Vol. 88, Issue 6
Production of Diethyl Terephthalate from Biomass-Derived Muconic Acid
journal, November 2015
- Lu, Rui; Lu, Fang; Chen, Jiazhi
- Angewandte Chemie International Edition, Vol. 55, Issue 1
Use of Pseudomonas spp. for the bioremediation of environmental pollutants: a review
journal, March 2013
- Wasi, Samina; Tabrez, Shams; Ahmad, Masood
- Environmental Monitoring and Assessment, Vol. 185, Issue 10
Lignin Valorization: Improving Lignin Processing in the Biorefinery
journal, May 2014
- Ragauskas, A. J.; Beckham, G. T.; Biddy, M. J.
- Science, Vol. 344, Issue 6185, p. 1246843-1246843
The contribution of proteomics to the unveiling of the survival strategies used by Pseudomonas putida in changing and hostile environments
journal, June 2013
- Moreno, Renata; Rojo, Fernando
- PROTEOMICS, Vol. 13, Issue 18-19
Pathways for degradation of lignin in bacteria and fungi
journal, January 2011
- Bugg, Timothy D. H.; Ahmad, Mark; Hardiman, Elizabeth M.
- Natural Product Reports, Vol. 28, Issue 12, p. 1883-1896
Reductive Catalytic Fractionation of Corn Stover Lignin
journal, October 2016
- Anderson, Eric M.; Katahira, Rui; Reed, Michelle
- ACS Sustainable Chemistry & Engineering, Vol. 4, Issue 12
Bioprivileged molecules: creating value from biomass
journal, January 2017
- Shanks, Brent H.; Keeling, Peter L.
- Green Chemistry, Vol. 19, Issue 14
Correction: Production of hydroxycinnamoyl anthranilates from glucose in Escherichia coli
journal, January 2014
- Eudes, Aymerick; Juminaga, Darmawi; Baidoo, Edward E. K.
- Microbial Cell Factories, Vol. 13, Issue 1
Base-Catalyzed Depolymerization of Biorefinery Lignins
journal, December 2015
- Katahira, Rui; Mittal, Ashutosh; McKinney, Kellene
- ACS Sustainable Chemistry & Engineering, Vol. 4, Issue 3
Renewable Unsaturated Polyesters from Muconic Acid
journal, October 2016
- Rorrer, Nicholas A.; Dorgan, John R.; Vardon, Derek R.
- ACS Sustainable Chemistry & Engineering, Vol. 4, Issue 12
The Catalytic Valorization of Lignin for the Production of Renewable Chemicals
journal, June 2010
- Zakzeski, Joseph; Bruijnincx, Pieter C. A.; Jongerius, Anna L.
- Chemical Reviews, Vol. 110, Issue 6, p. 3552-3599
Opportunities and challenges in biological lignin valorization
journal, December 2016
- Beckham, Gregg T.; Johnson, Christopher W.; Karp, Eric M.
- Current Opinion in Biotechnology, Vol. 42
Adipic acid production from lignin
journal, January 2015
- Vardon, Derek R.; Franden, Mary Ann; Johnson, Christopher W.
- Energy & Environmental Science, Vol. 8, Issue 2
Alkaline Pretreatment of Switchgrass
journal, May 2015
- Karp, Eric M.; Resch, Michael G.; Donohoe, Bryon S.
- ACS Sustainable Chemistry & Engineering, Vol. 3, Issue 7
Production of hydroxycinnamoyl anthranilates from glucose in Escherichia coli
journal, January 2013
- Eudes, Aymerick; Juminaga, Darmawi; Baidoo, Edward E. K.
- Microbial Cell Factories, Vol. 12, Issue 1
Comparison of submerged and solid state pretreatment of sugarcane bagasse by Pandoraea sp. ISTKB: Enzymatic and structural analysis
journal, March 2016
- Kumar, Madan; Singhal, Anjali; Thakur, Indu Shekhar
- Bioresource Technology, Vol. 203
Total Utilization of Miscanthus Biomass, Lignin and Carbohydrates, Using Earth Abundant Nickel Catalyst
journal, February 2016
- Luo, Hao; Klein, Ian M.; Jiang, Yuan
- ACS Sustainable Chemistry & Engineering, Vol. 4, Issue 4
DMR (deacetylation and mechanical refining) processing of corn stover achieves high monomeric sugar concentrations (230 g L −1 ) during enzymatic hydrolysis and high ethanol concentrations (>10% v/v) during fermentation without hydrolysate purification or concentration
journal, January 2016
- Chen, Xiaowen; Kuhn, Erik; Jennings, Edward W.
- Energy & Environmental Science, Vol. 9, Issue 4
Synergistic enzymatic and microbial lignin conversion
journal, January 2016
- Zhao, Cheng; Xie, Shangxian; Pu, Yunqiao
- Green Chemistry, Vol. 18, Issue 5
Enhancing muconic acid production from glucose and lignin-derived aromatic compounds via increased protocatechuate decarboxylase activity
journal, December 2016
- Johnson, Christopher W.; Salvachúa, Davinia; Khanna, Payal
- Metabolic Engineering Communications, Vol. 3
Paving the Way for Lignin Valorisation: Recent Advances in Bioengineering, Biorefining and Catalysis
journal, June 2016
- Rinaldi, Roberto; Jastrzebski, Robin; Clough, Matthew T.
- Angewandte Chemie International Edition, Vol. 55, Issue 29
Potential applications of ferulic acid from natural sources
journal, December 2014
- Kumar, Naresh; Pruthi, Vikas
- Biotechnology Reports, Vol. 4
Recent Development in Chemical Depolymerization of Lignin: A Review
journal, January 2013
- Wang, Hai; Tucker, Melvin; Ji, Yun
- Journal of Applied Chemistry, Vol. 2013, p. 1-9
Regulation of Aromatic Metabolism in the Fungi: Metabolic Control of the 3-Oxoadipate Pathway in the Yeast Rhodotorula mucilaginosa
journal, November 1974
- Cook, K. A.; Cain, R. B.
- Journal of General Microbiology, Vol. 85, Issue 1
Hydroxycinnamates in lignification
journal, August 2009
- Ralph, John
- Phytochemistry Reviews, Vol. 9, Issue 1, p. 65-83
Biotransformations Catalyzed by the Genus Rhodococcus
journal, January 1994
- Warhurst, A. Michael; Fewson, Charles A.
- Critical Reviews in Biotechnology, Vol. 14, Issue 1
Potential applications of ferulic acid from natural sources
journal, December 2014
- Kumar, Naresh; Pruthi, Vikas
- Biotechnology Reports, Vol. 4
Enhancing muconic acid production from glucose and lignin-derived aromatic compounds via increased protocatechuate decarboxylase activity
journal, December 2016
- Johnson, Christopher W.; Salvachúa, Davinia; Khanna, Payal
- Metabolic Engineering Communications, Vol. 3
Tricin-lignins: occurrence and quantitation of tricin in relation to phylogeny
journal, November 2016
- Lan, Wu; Rencoret, Jorge; Lu, Fachuang
- The Plant Journal, Vol. 88, Issue 6
Lignin Valorization: Improving Lignin Processing in the Biorefinery
journal, May 2014
- Ragauskas, A. J.; Beckham, G. T.; Biddy, M. J.
- Science, Vol. 344, Issue 6185, p. 1246843-1246843
Works referencing / citing this record:
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journal, September 2018
- Ravi, Krithika; Abdelaziz, Omar Y.; Nöbel, Matthias
- Biotechnology for Biofuels, Vol. 11, Issue 1
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journal, March 2018
- Ravi, Krithika; García-Hidalgo, Javier; Nöbel, Matthias
- AMB Express, Vol. 8, Issue 1
Integrated diesel production from lignocellulosic sugars via oleaginous yeast
journal, January 2018
- Sànchez i. Nogué, Violeta; Black, Brenna A.; Kruger, Jacob S.
- Green Chemistry, Vol. 20, Issue 18
Metabolic engineering of Pseudomonas putida for increased polyhydroxyalkanoate production from lignin
journal, January 2020
- Salvachúa, Davinia; Rydzak, Thomas; Auwae, Raquel
- Microbial Biotechnology, Vol. 13, Issue 1
Isolation of a novel platform bacterium for lignin valorization and its application in glucose-free cis,cis-muconate production
journal, May 2019
- Shinoda, Eri; Takahashi, Kenji; Abe, Nanase
- Journal of Industrial Microbiology & Biotechnology, Vol. 46, Issue 8
Efficient conversion of lignin into a water-soluble polymer by a chelator-mediated Fenton reaction: optimization of H 2 O 2 use and performance as a dispersant
journal, January 2018
- Kent, Michael S.; Zeng, Jijiao; Rader, Nadeya
- Green Chemistry, Vol. 20, Issue 13
Bioprocess development for muconic acid production from aromatic compounds and lignin
journal, January 2018
- Salvachúa, Davinia; Johnson, Christopher W.; Singer, Christine A.
- Green Chemistry, Vol. 20, Issue 21
Alkane and wax ester production from lignin‐related aromatic compounds
journal, May 2019
- Salmela, Milla; Lehtinen, Tapio; Efimova, Elena
- Biotechnology and Bioengineering, Vol. 116, Issue 8
Plasma-deposition of α-FeOOH particles on biochar using a gliding arc discharge in humid air: a green and sustainable route for producing oxidation catalysts
journal, January 2019
- Tiya-Djowe, Antoine; Dourges, Marie-Anne; Bruneel, Jean-Luc
- RSC Advances, Vol. 9, Issue 9
Across the Board: Mark Mascal on the Challenges of Lignin Biorefining
journal, December 2019
- Mascal, Mark
- ChemSusChem, Vol. 13, Issue 1
Alternatives for Chemical and Biochemical Lignin Valorization: Hot Topics from a Bibliometric Analysis of the Research Published During the 2000–2016 Period
journal, July 2018
- Abejón, Ricardo; Pérez-Acebo, Heriberto; Clavijo, Leonardo
- Processes, Vol. 6, Issue 8
Restoration of biofuel production levels and increased tolerance under ionic liquid stress is enabled by a mutation in the essential Escherichia coli gene cydC
text, January 2018
- Eng, Thomas; Demling, Philipp; Herbert, Robin A.
- RWTH Aachen University
High value‐added monomer chemicals and functional bio‐based materials derived from polymeric components of lignocellulose by organosolv fractionation
journal, November 2019
- Zhang, Jingzhi; Cai, Di; Qin, Yanlin
- Biofuels, Bioproducts and Biorefining
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journal, October 2018
- Eng, Thomas; Demling, Philipp; Herbert, Robin A.
- Microbial Cell Factories, Vol. 17, Issue 1
Engineering Corynebacterium glutamicum to produce the biogasoline isopentenol from plant biomass hydrolysates
journal, February 2019
- Sasaki, Yusuke; Eng, Thomas; Herbert, Robin A.
- Biotechnology for Biofuels, Vol. 12, Issue 1
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journal, March 2019
- Ravi, Krithika; Abdelaziz, Omar Y.; Nöbel, Matthias
- Biotechnology for Biofuels, Vol. 12, Issue 1
Identification of parallel and divergent optimization solutions for homologous metabolic enzymes
journal, June 2018
- Standaert, Robert F.; Giannone, Richard J.; Michener, Joshua K.
- Metabolic Engineering Communications, Vol. 6
Vanillin Production in Pseudomonas : Whole-Genome Sequencing of Pseudomonas sp. Strain 9.1 and Reannotation of Pseudomonas putida CalA as a Vanillin Reductase
journal, January 2020
- García-Hidalgo, Javier; Brink, Daniel P.; Ravi, Krithika
- Applied and Environmental Microbiology, Vol. 86, Issue 6
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journal, October 2018
- Eng, Thomas; Demling, Philipp; Herbert, Robin A.
- Microbial Cell Factories, Vol. 17, Issue 1
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journal, March 2019
- Ravi, Krithika; Abdelaziz, Omar Y.; Nöbel, Matthias
- Biotechnology for Biofuels, Vol. 12, Issue 1
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journal, March 2018
- Ravi, Krithika; García-Hidalgo, Javier; Nöbel, Matthias
- AMB Express, Vol. 8, Issue 1
Bacterial Valorization of Lignin: Strains, Enzymes, Conversion Pathways, Biosensors, and Perspectives
journal, September 2019
- Lee, Siseon; Kang, Minsik; Bae, Jung-Hoon
- Frontiers in Bioengineering and Biotechnology, Vol. 7
Effects of Process Parameters on Hydrolytic Treatment of Black Liquor for the Production of Low-Molecular-Weight Depolymerized Kraft Lignin
journal, September 2018
- Ahmad, Zaid; Mahmood, Nubla; Yuan, Zhongshun
- Molecules, Vol. 23, Issue 10