Structure of Fission Yeast Transcription Factor Pho7 Bound to pho1 Promoter DNA and Effect of Pho7 Mutations on DNA Binding and Phosphate Homeostasis
Abstract
Pho7 is the Schizosaccharomyces pombe fission yeast Zn2Cys6 transcriptional factor that drives a response to phosphate starvation in which phosphate acquisition genes are upregulated. Here we report a crystal structure at 1.6-Å resolution of the Pho7 DNA-binding domain (DBD) bound at its target site 2 in the pho1 promoter (5'-TCGGAAATTAAAAA). Comparison to the previously reported structure of Pho7 DBD in complex with its binding site in the tgp1 promoter (5'-TCGGACATTCAAAT) reveals shared determinants of target site specificity as well as variations in the protein-DNA interface that accommodate different promoter DNA sequences. Mutagenesis of Pho7 amino acids at the DNA interface identified nucleobase contacts at the periphery of the footprint that are essential for the induction of pho1 expression in response to phosphate starvation and for Pho7 binding to site 1 in the pho1 promoter.
- Authors:
-
- Sloan-Kettering Inst., New York, NY (United States)
- Cornell Univ., Ithaca, NY (United States). Weill Medical College
- Publication Date:
- Research Org.:
- Argonne National Lab. (ANL), Argonne, IL (United States)
- Sponsoring Org.:
- National Institutes of Health (NIH)
- OSTI Identifier:
- 1543005
- Grant/Contract Number:
- R35-GM126945; R01- GM52470; P30-CA008748; P41GM103403; HEI-S10RR029205; AC02-06CH11357
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Molecular and Cellular Biology
- Additional Journal Information:
- Journal Volume: 39; Journal Issue: 13; Journal ID: ISSN 0270-7306
- Publisher:
- American Society for Microbiology
- Country of Publication:
- United States
- Language:
- ENGLISH
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; DNA-protein interaction; Schizosaccharomyces pombe; mutagenesis; protein structure; transcription factor
Citation Formats
Garg, Angad, Goldgur, Yehuda, Sanchez, Ana M., Schwer, Beate, and Shuman, Stewart. Structure of Fission Yeast Transcription Factor Pho7 Bound to pho1 Promoter DNA and Effect of Pho7 Mutations on DNA Binding and Phosphate Homeostasis. United States: N. p., 2019.
Web. doi:10.1128/MCB.00132-19.
Garg, Angad, Goldgur, Yehuda, Sanchez, Ana M., Schwer, Beate, & Shuman, Stewart. Structure of Fission Yeast Transcription Factor Pho7 Bound to pho1 Promoter DNA and Effect of Pho7 Mutations on DNA Binding and Phosphate Homeostasis. United States. https://doi.org/10.1128/MCB.00132-19
Garg, Angad, Goldgur, Yehuda, Sanchez, Ana M., Schwer, Beate, and Shuman, Stewart. Mon .
"Structure of Fission Yeast Transcription Factor Pho7 Bound to pho1 Promoter DNA and Effect of Pho7 Mutations on DNA Binding and Phosphate Homeostasis". United States. https://doi.org/10.1128/MCB.00132-19. https://www.osti.gov/servlets/purl/1543005.
@article{osti_1543005,
title = {Structure of Fission Yeast Transcription Factor Pho7 Bound to pho1 Promoter DNA and Effect of Pho7 Mutations on DNA Binding and Phosphate Homeostasis},
author = {Garg, Angad and Goldgur, Yehuda and Sanchez, Ana M. and Schwer, Beate and Shuman, Stewart},
abstractNote = {Pho7 is the Schizosaccharomyces pombe fission yeast Zn2Cys6 transcriptional factor that drives a response to phosphate starvation in which phosphate acquisition genes are upregulated. Here we report a crystal structure at 1.6-Å resolution of the Pho7 DNA-binding domain (DBD) bound at its target site 2 in the pho1 promoter (5'-TCGGAAATTAAAAA). Comparison to the previously reported structure of Pho7 DBD in complex with its binding site in the tgp1 promoter (5'-TCGGACATTCAAAT) reveals shared determinants of target site specificity as well as variations in the protein-DNA interface that accommodate different promoter DNA sequences. Mutagenesis of Pho7 amino acids at the DNA interface identified nucleobase contacts at the periphery of the footprint that are essential for the induction of pho1 expression in response to phosphate starvation and for Pho7 binding to site 1 in the pho1 promoter.},
doi = {10.1128/MCB.00132-19},
journal = {Molecular and Cellular Biology},
number = 13,
volume = 39,
place = {United States},
year = {Mon Apr 22 00:00:00 EDT 2019},
month = {Mon Apr 22 00:00:00 EDT 2019}
}
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