WRINKLED1 regulates BIOTIN ATTACHMENT DOMAIN-CONTAINING proteins that inhibit fatty acid synthesis
Abstract
WRINKLED1 (WRI1) is a transcriptional activator that binds to a conserved sequence (designated as AW box) boxes in the promoters of many genes from central metabolism and fatty acid (FA) synthesis, resulting in their transcription. BIOTIN ATTACHMENT DOMAIN-CONTAINING (BADC) proteins lack a biotin-attachment domain and are therefore inactive, but in the presence of excess FA, BADC1 and BADC3 are primarily responsible for the observed long-term irreversible inhibition of ACETYL-COA CARBOXYLASE, and consequently FA synthesis. Here, we tested the interaction of WRI1 with BADC genes in Arabidopsis (Arabidopsis thaliana) and found purified WRI1 bound with high affinity to canonical AW boxes from the promoters of all three BADC genes. Consistent with this observation, both expression of BADC1, BADC2, and BADC3 genes and BADC1 protein levels were reduced in wri1-1 relative to the wild type, and elevated upon WRI1 overexpression. The double mutant badc1 badc2 phenocopied wri1-1 with respect to both reduction in root length and elevation of indole-3-acetic acid-Asp levels relative to the wild type. Overexpression of BADC1 in wri1-1 decreased indole-3-acetic acid-Asp content and partially rescued its short-root phenotype, demonstrating a role for BADCs in seedling establishment. That WRI1 positively regulates genes encoding both FA synthesis and BADC proteins (i.e.more »
- Authors:
- Publication Date:
- Research Org.:
- Brookhaven National Laboratory (BNL), Upton, NY (United States)
- Sponsoring Org.:
- USDOE Office of Science (SC), Basic Energy Sciences (BES)
- OSTI Identifier:
- 1526665
- Alternate Identifier(s):
- OSTI ID: 1525402
- Report Number(s):
- BNL-211766-2019-JAAM
Journal ID: ISSN 0032-0889; plantphysiol;pp.19.00587v1
- Grant/Contract Number:
- SC0012704
- Resource Type:
- Published Article
- Journal Name:
- Plant Physiology (Bethesda)
- Additional Journal Information:
- Journal Name: Plant Physiology (Bethesda); Journal ID: ISSN 0032-0889
- Publisher:
- American Society of Plant Biologists (ASPB)
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Metabolic regulation; homeostatic mechanism; lipid metabolism; triacylglycerol
Citation Formats
Liu, Hui, Zhai, Zhiyang, Kuczynski, Kate, Keereetaweep, Jantana, Schwender, Jorg, and Shanklin, John. WRINKLED1 regulates BIOTIN ATTACHMENT DOMAIN-CONTAINING proteins that inhibit fatty acid synthesis. United States: N. p., 2019.
Web. doi:10.1104/pp.19.00587.
Liu, Hui, Zhai, Zhiyang, Kuczynski, Kate, Keereetaweep, Jantana, Schwender, Jorg, & Shanklin, John. WRINKLED1 regulates BIOTIN ATTACHMENT DOMAIN-CONTAINING proteins that inhibit fatty acid synthesis. United States. https://doi.org/10.1104/pp.19.00587
Liu, Hui, Zhai, Zhiyang, Kuczynski, Kate, Keereetaweep, Jantana, Schwender, Jorg, and Shanklin, John. Mon .
"WRINKLED1 regulates BIOTIN ATTACHMENT DOMAIN-CONTAINING proteins that inhibit fatty acid synthesis". United States. https://doi.org/10.1104/pp.19.00587.
@article{osti_1526665,
title = {WRINKLED1 regulates BIOTIN ATTACHMENT DOMAIN-CONTAINING proteins that inhibit fatty acid synthesis},
author = {Liu, Hui and Zhai, Zhiyang and Kuczynski, Kate and Keereetaweep, Jantana and Schwender, Jorg and Shanklin, John},
abstractNote = {WRINKLED1 (WRI1) is a transcriptional activator that binds to a conserved sequence (designated as AW box) boxes in the promoters of many genes from central metabolism and fatty acid (FA) synthesis, resulting in their transcription. BIOTIN ATTACHMENT DOMAIN-CONTAINING (BADC) proteins lack a biotin-attachment domain and are therefore inactive, but in the presence of excess FA, BADC1 and BADC3 are primarily responsible for the observed long-term irreversible inhibition of ACETYL-COA CARBOXYLASE, and consequently FA synthesis. Here, we tested the interaction of WRI1 with BADC genes in Arabidopsis (Arabidopsis thaliana) and found purified WRI1 bound with high affinity to canonical AW boxes from the promoters of all three BADC genes. Consistent with this observation, both expression of BADC1, BADC2, and BADC3 genes and BADC1 protein levels were reduced in wri1-1 relative to the wild type, and elevated upon WRI1 overexpression. The double mutant badc1 badc2 phenocopied wri1-1 with respect to both reduction in root length and elevation of indole-3-acetic acid-Asp levels relative to the wild type. Overexpression of BADC1 in wri1-1 decreased indole-3-acetic acid-Asp content and partially rescued its short-root phenotype, demonstrating a role for BADCs in seedling establishment. That WRI1 positively regulates genes encoding both FA synthesis and BADC proteins (i.e. conditional inhibitors of FA synthesis), represents a coordinated mechanism to achieve lipid homeostasis in which plants couple the transcription of their FA synthetic capacity with their capacity to biochemically downregulate it.},
doi = {10.1104/pp.19.00587},
journal = {Plant Physiology (Bethesda)},
number = ,
volume = ,
place = {United States},
year = {Mon Jun 17 00:00:00 EDT 2019},
month = {Mon Jun 17 00:00:00 EDT 2019}
}
https://doi.org/10.1104/pp.19.00587
Search WorldCat to find libraries that may hold this journalWeb of Science