skip to main content

DOE PAGESDOE PAGES

Title: The Arabidopsis WRINKLED1 transcription factor affects auxin homeostasis in roots

WRINKLED1 (WRI1) is a key transcriptional regulator of fatty acid biosynthesis genes in diverse oil-containing tissues. Loss of function of Arabidopsis WRI1 leads to a reduction in the expression of genes for fatty acid biosynthesis and glycolysis, and concomitant strong reduction of seed oil content. The wri1-1 loss-of-function mutant shows reduced primary root growth and decreased acidification of the growth medium. The content of a conjugated form of the plant growth hormone auxin, indole-3-acetic acid (IAA)-Asp, was higher in wri1-1 plants compared with the wild-type. GH3.3, a gene encoding an enzyme involved in auxin degradation, displayed higher expression in the wri1-1 mutant. EMSAs demonstrated that AtWRI1 bound to the promoter of GH3.3. Specific AtWRI1-binding motifs were identified in the promoter of GH3.3. In addition, wri1-1 displayed decreased auxin transport. Expression of some PIN genes, which encode IAA carrier proteins, was reduced in wri1-1 plants as well. Correspondingly, AtWRI1 bound to the promoter regions of some PIN genes. It is well known that auxin exerts its maximum effects at a specific, optimal concentration in roots requiring a finely balanced auxin homeostasis. This process appears to be disrupted when the expression of WRI1 and in turn a subset of its target genesmore » are misregulated, highlighting a role for WRI1 in root auxin homeostasis.« less
Authors:
 [1] ;  [2] ;  [3] ;  [3] ;  [4] ; ORCiD logo [5]
  1. Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Laboratory, Department of Biochemistry and Molecular Biology, and Great Lakes Bioenergy Research Center
  2. Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Laboratory, Great Lakes Bioenergy Research Center, and Department of Plant Biology
  3. Purdue Univ., West Lafayette, IN (United States). Department of Horticulture and Landscape Architecture
  4. Michigan State Univ., East Lansing, MI (United States). Department of Biochemistry and Molecular Biology
  5. Michigan State Univ., East Lansing, MI (United States). MSU-DOE Plant Research Laboratory, Department of Biochemistry and Molecular Biology, Great Lakes Bioenergy Research Center and Department of Plant Biology
Publication Date:
Grant/Contract Number:
FC02-07ER64494
Type:
Accepted Manuscript
Journal Name:
Journal of Experimental Botany
Additional Journal Information:
Journal Volume: 68; Journal Issue: 16; Journal ID: ISSN 0022-0957
Publisher:
Oxford University Press
Research Org:
Univ. of Wisconsin, Madison, WI (United States)
Sponsoring Org:
USDOE
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Arabidopsis; auxin; DNA-binding motif; gene expression; transcription factor; root development
OSTI Identifier:
1427578

Kong, Que, Ma, Wei, Yang, Haibing, Ma, Guojie, Mantyla, Jenny J., and Benning, Christoph. The Arabidopsis WRINKLED1 transcription factor affects auxin homeostasis in roots. United States: N. p., Web. doi:10.1093/jxb/erx275.
Kong, Que, Ma, Wei, Yang, Haibing, Ma, Guojie, Mantyla, Jenny J., & Benning, Christoph. The Arabidopsis WRINKLED1 transcription factor affects auxin homeostasis in roots. United States. doi:10.1093/jxb/erx275.
Kong, Que, Ma, Wei, Yang, Haibing, Ma, Guojie, Mantyla, Jenny J., and Benning, Christoph. 2017. "The Arabidopsis WRINKLED1 transcription factor affects auxin homeostasis in roots". United States. doi:10.1093/jxb/erx275. https://www.osti.gov/servlets/purl/1427578.
@article{osti_1427578,
title = {The Arabidopsis WRINKLED1 transcription factor affects auxin homeostasis in roots},
author = {Kong, Que and Ma, Wei and Yang, Haibing and Ma, Guojie and Mantyla, Jenny J. and Benning, Christoph},
abstractNote = {WRINKLED1 (WRI1) is a key transcriptional regulator of fatty acid biosynthesis genes in diverse oil-containing tissues. Loss of function of Arabidopsis WRI1 leads to a reduction in the expression of genes for fatty acid biosynthesis and glycolysis, and concomitant strong reduction of seed oil content. The wri1-1 loss-of-function mutant shows reduced primary root growth and decreased acidification of the growth medium. The content of a conjugated form of the plant growth hormone auxin, indole-3-acetic acid (IAA)-Asp, was higher in wri1-1 plants compared with the wild-type. GH3.3, a gene encoding an enzyme involved in auxin degradation, displayed higher expression in the wri1-1 mutant. EMSAs demonstrated that AtWRI1 bound to the promoter of GH3.3. Specific AtWRI1-binding motifs were identified in the promoter of GH3.3. In addition, wri1-1 displayed decreased auxin transport. Expression of some PIN genes, which encode IAA carrier proteins, was reduced in wri1-1 plants as well. Correspondingly, AtWRI1 bound to the promoter regions of some PIN genes. It is well known that auxin exerts its maximum effects at a specific, optimal concentration in roots requiring a finely balanced auxin homeostasis. This process appears to be disrupted when the expression of WRI1 and in turn a subset of its target genes are misregulated, highlighting a role for WRI1 in root auxin homeostasis.},
doi = {10.1093/jxb/erx275},
journal = {Journal of Experimental Botany},
number = 16,
volume = 68,
place = {United States},
year = {2017},
month = {8}
}