In situ liquid cell crystallization and imaging of thiamethoxam by helium ion microscopy

Belianinov, Alex; Pawlicki, Alison A.; Burch, Matt J.; Kim, Songkil; Ievlev, Anton; Fowler, Jeff; Ovchinnikova, Olga S.

doi:10.1116/1.5040849

Title: In situ liquid cell crystallization and imaging of thiamethoxam by helium ion microscopy

Abstract

Pest insect control is an essential component for crop protection and public health. Neonicotinoids are a relatively new class of insecticides developed in the last four decades. Thiamethoxam, a member of the neonicotinoid class, has shown outstanding potency for crop protection against a variety of piercing-sucking pests. However, its use in industrial-volume packing and transportation is complicated by crystallization dynamics. In this work, a helium ion microscope (HIM) with a Protochips liquid cell was utilized to crystallize and image thiamethoxam in situ. The results of the study illustrate the growth and morphology of the thiamethoxam crystals at different He⁺ exposure doses, which is markedly different from what has been typically observed. Energy-dispersive x-ray spectroscopy results confirm the presence of the thiamethoxam on the liquid cell membrane. This imaging study illustrates the HIM ability to image and induce the crystallization in soft materials in liquid environments, and attempts to shed light onto the key processes involved in liquid imaging.

Authors:

^[1]; Pawlicki, Alison A. ^[2];

^[1];

^[3];

^[1]; Fowler, Jeff ^[4];

^[1]

Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States)
Pusan National Univ., Busan (South Korea)
Syngenta Crop Protection, Greensboro, NC (United States)

Publication Date:: Fri Aug 31 00:00:00 EDT 2018

Research Org.:: Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Sponsoring Org.:: USDOE

OSTI Identifier:: 1524876

Grant/Contract Number:: AC05-00OR22725

Resource Type:: Accepted Manuscript

Journal Name:: Journal of Vacuum Science and Technology. B, Nanotechnology and Microelectronics

Additional Journal Information:: Journal Volume: 36; Journal Issue: 5; Journal ID: ISSN 2166-2746

Publisher:: American Vacuum Society/AIP

Country of Publication:: United States

Language:: English

Subject:: 60 APPLIED LIFE SCIENCES; 77 NANOSCIENCE AND NANOTECHNOLOGY

Citation Formats


                    Belianinov, Alex, Pawlicki, Alison A., Burch, Matt J., Kim, Songkil, Ievlev, Anton, Fowler, Jeff, and Ovchinnikova, Olga S. In situ liquid cell crystallization and imaging of thiamethoxam by helium ion microscopy.  United States: N. p., 2018. 
Web.  doi:10.1116/1.5040849.

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                    Belianinov, Alex, Pawlicki, Alison A., Burch, Matt J., Kim, Songkil, Ievlev, Anton, Fowler, Jeff, & Ovchinnikova, Olga S. In situ liquid cell crystallization and imaging of thiamethoxam by helium ion microscopy.  United States.  https://doi.org/10.1116/1.5040849

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                    Belianinov, Alex, Pawlicki, Alison A., Burch, Matt J., Kim, Songkil, Ievlev, Anton, Fowler, Jeff, and Ovchinnikova, Olga S. Fri .  
"In situ liquid cell crystallization and imaging of thiamethoxam by helium ion microscopy".  United States.  https://doi.org/10.1116/1.5040849.  https://www.osti.gov/servlets/purl/1524876.

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@article{osti_1524876,

  title        = {In situ liquid cell crystallization and imaging of thiamethoxam by helium ion microscopy},

  author       = {Belianinov, Alex and Pawlicki, Alison A. and Burch, Matt J. and Kim, Songkil and Ievlev, Anton and Fowler, Jeff and Ovchinnikova, Olga S.},

  abstractNote = {Pest insect control is an essential component for crop protection and public health. Neonicotinoids are a relatively new class of insecticides developed in the last four decades. Thiamethoxam, a member of the neonicotinoid class, has shown outstanding potency for crop protection against a variety of piercing-sucking pests. However, its use in industrial-volume packing and transportation is complicated by crystallization dynamics. In this work, a helium ion microscope (HIM) with a Protochips liquid cell was utilized to crystallize and image thiamethoxam in situ. The results of the study illustrate the growth and morphology of the thiamethoxam crystals at different He+ exposure doses, which is markedly different from what has been typically observed. Energy-dispersive x-ray spectroscopy results confirm the presence of the thiamethoxam on the liquid cell membrane. This imaging study illustrates the HIM ability to image and induce the crystallization in soft materials in liquid environments, and attempts to shed light onto the key processes involved in liquid imaging.},

  doi          = {10.1116/1.5040849},

  journal      = {Journal of Vacuum Science and Technology. B, Nanotechnology and Microelectronics},

  number       = 5,

  volume       = 36,

  place        = {United States},

  year         = {Fri Aug 31 00:00:00 EDT 2018},

  month        = {Fri Aug 31 00:00:00 EDT 2018}

}

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Figures / Tables:

FIG. 1: HIM, with the use of e⁻flood gun, displaying TMX crystals on a glass surface. (a) HIM image of many large crystallites that form upon drop casting an aqueous supersaturated solution of TMX and drying. (b) HIM image of two particular TMX crystals. (c) HIM image with amore »

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