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Title: Morphological and chemical evidence for cyclic bone growth in a fossil hyaena

Abstract

Trace element inventories are known to correlate with specific histological structures in bone, reflecting organismal physiology and life histories. By studying trace elements in fossilised bone, particularly in individuals with cyclic bone growth (alternating fast/slow bone deposition), we can improve our understanding of the physiology of extinct organisms. In this study we present the first direct comparison between optical histology (bone tissue identification) and synchrotron-based chemical mapping, quantification, and characterisation of trace elements (biochemistry) within cyclic growth tissues, in this case within bones of a cave hyaena ( Crocuta crocuta spelaea). Results show distributions of zinc, an element strongly associated with active ossification and bone growth, correlating with (1) fast-growing tissue of zonal bone (cyclic growth) in an extinct hyaena and (2) secondary osteons (remodelling) in both extant and extinct hyaena. Concentrations and coordination chemistry of zinc within the fossil sample are comparable to those seen in extant bone suggesting that zinc is endogenous to the sample and that the chemistry of bone growth has been preserved for 40 ka. Furthermore, these results demonstrate that the study of trace elements as part of the histochemistry has wide utility for reconstructing growth, diet and other lifestyle factors in archaeological and fossilmore » bone.« less

Authors:
ORCiD logo [1];  [2]; ORCiD logo [3];  [4];  [2];  [2];  [5];  [5];  [5]; ORCiD logo [2];  [1];  [1]
  1. Univ. of Manchester, Manchester (United Kingdom); The Children's Museum of Indianapolis, Indianapolis, IN (United States)
  2. Univ. of Manchester, Manchester (United Kingdom)
  3. Univ. of Manchester, Manchester (United Kingdom); SLAC National Accelerator Lab., Menlo Park, CA (United States)
  4. Univ. of Manchester, Manchester (United Kingdom); Univ. of Southampton, Southampton (United Kingdom)
  5. SLAC National Accelerator Lab., Menlo Park, CA (United States)
Publication Date:
Research Org.:
SLAC National Accelerator Lab., Menlo Park, CA (United States)
Sponsoring Org.:
USDOE
OSTI Identifier:
1490883
Alternate Identifier(s):
OSTI ID: 1478122
Grant/Contract Number:  
NE/J023426/1; ST/M001814/1; Deans Award; UF120473; AC02-76SF00515; P41GM103393
Resource Type:
Accepted Manuscript
Journal Name:
Journal of Analytical Atomic Spectrometry
Additional Journal Information:
Journal Volume: 33; Journal Issue: 12; Journal ID: ISSN 0267-9477
Publisher:
Royal Society of Chemistry
Country of Publication:
United States
Language:
English
Subject:
37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; 59 BASIC BIOLOGICAL SCIENCES

Citation Formats

Anné, Jennifer, Wogelius, Roy A., Edwards, Nicholas P., van Veelen, Arjen, Buckley, Michael, Sellers, William I., Bergmann, Uwe, Sokaras, Dimosthenis, Alonso-Mori, Roberto, Harvey, Virginia L., Egerton, Victoria M., and Manning, Phillip L. Morphological and chemical evidence for cyclic bone growth in a fossil hyaena. United States: N. p., 2018. Web. doi:10.1039/c8ja00314a.
Anné, Jennifer, Wogelius, Roy A., Edwards, Nicholas P., van Veelen, Arjen, Buckley, Michael, Sellers, William I., Bergmann, Uwe, Sokaras, Dimosthenis, Alonso-Mori, Roberto, Harvey, Virginia L., Egerton, Victoria M., & Manning, Phillip L. Morphological and chemical evidence for cyclic bone growth in a fossil hyaena. United States. doi:10.1039/c8ja00314a.
Anné, Jennifer, Wogelius, Roy A., Edwards, Nicholas P., van Veelen, Arjen, Buckley, Michael, Sellers, William I., Bergmann, Uwe, Sokaras, Dimosthenis, Alonso-Mori, Roberto, Harvey, Virginia L., Egerton, Victoria M., and Manning, Phillip L. Thu . "Morphological and chemical evidence for cyclic bone growth in a fossil hyaena". United States. doi:10.1039/c8ja00314a. https://www.osti.gov/servlets/purl/1490883.
@article{osti_1490883,
title = {Morphological and chemical evidence for cyclic bone growth in a fossil hyaena},
author = {Anné, Jennifer and Wogelius, Roy A. and Edwards, Nicholas P. and van Veelen, Arjen and Buckley, Michael and Sellers, William I. and Bergmann, Uwe and Sokaras, Dimosthenis and Alonso-Mori, Roberto and Harvey, Virginia L. and Egerton, Victoria M. and Manning, Phillip L.},
abstractNote = {Trace element inventories are known to correlate with specific histological structures in bone, reflecting organismal physiology and life histories. By studying trace elements in fossilised bone, particularly in individuals with cyclic bone growth (alternating fast/slow bone deposition), we can improve our understanding of the physiology of extinct organisms. In this study we present the first direct comparison between optical histology (bone tissue identification) and synchrotron-based chemical mapping, quantification, and characterisation of trace elements (biochemistry) within cyclic growth tissues, in this case within bones of a cave hyaena (Crocuta crocuta spelaea). Results show distributions of zinc, an element strongly associated with active ossification and bone growth, correlating with (1) fast-growing tissue of zonal bone (cyclic growth) in an extinct hyaena and (2) secondary osteons (remodelling) in both extant and extinct hyaena. Concentrations and coordination chemistry of zinc within the fossil sample are comparable to those seen in extant bone suggesting that zinc is endogenous to the sample and that the chemistry of bone growth has been preserved for 40 ka. Furthermore, these results demonstrate that the study of trace elements as part of the histochemistry has wide utility for reconstructing growth, diet and other lifestyle factors in archaeological and fossil bone.},
doi = {10.1039/c8ja00314a},
journal = {Journal of Analytical Atomic Spectrometry},
number = 12,
volume = 33,
place = {United States},
year = {2018},
month = {10}
}

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