Morphological and chemical evidence for cyclic bone growth in a fossil hyaena
Abstract
Trace element inventories are known to correlate with specific histological structures in bone, reflecting organismal physiology and life histories. By studying trace elements in fossilised bone, particularly in individuals with cyclic bone growth (alternating fast/slow bone deposition), we can improve our understanding of the physiology of extinct organisms. In this study we present the first direct comparison between optical histology (bone tissue identification) and synchrotron-based chemical mapping, quantification, and characterisation of trace elements (biochemistry) within cyclic growth tissues, in this case within bones of a cave hyaena (Crocuta crocuta spelaea). Results show distributions of zinc, an element strongly associated with active ossification and bone growth, correlating with (1) fast-growing tissue of zonal bone (cyclic growth) in an extinct hyaena and (2) secondary osteons (remodelling) in both extant and extinct hyaena. Concentrations and coordination chemistry of zinc within the fossil sample are comparable to those seen in extant bone suggesting that zinc is endogenous to the sample and that the chemistry of bone growth has been preserved for 40 ka. Furthermore, these results demonstrate that the study of trace elements as part of the histochemistry has wide utility for reconstructing growth, diet and other lifestyle factors in archaeological and fossil bone.
- Authors:
-
[1];
[3];
[2];
- Univ. of Manchester, Manchester (United Kingdom); The Children's Museum of Indianapolis, Indianapolis, IN (United States)
- Univ. of Manchester, Manchester (United Kingdom)
- Univ. of Manchester, Manchester (United Kingdom); SLAC National Accelerator Lab., Menlo Park, CA (United States)
- Univ. of Manchester, Manchester (United Kingdom); Univ. of Southampton, Southampton (United Kingdom)
- SLAC National Accelerator Lab., Menlo Park, CA (United States)
- Publication Date:
- Research Org.:
- SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)
- Sponsoring Org.:
- USDOE
- OSTI Identifier:
- 1490883
- Alternate Identifier(s):
- OSTI ID: 1478122
- Grant/Contract Number:
- NE/J023426/1; ST/M001814/1; Deans Award; UF120473; AC02-76SF00515; P41GM103393
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Journal of Analytical Atomic Spectrometry
- Additional Journal Information:
- Journal Volume: 33; Journal Issue: 12; Journal ID: ISSN 0267-9477
- Publisher:
- Royal Society of Chemistry
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY; 59 BASIC BIOLOGICAL SCIENCES
Citation Formats
Anné, Jennifer, Wogelius, Roy A., Edwards, Nicholas P., van Veelen, Arjen, Buckley, Michael, Sellers, William I., Bergmann, Uwe, Sokaras, Dimosthenis, Alonso-Mori, Roberto, Harvey, Virginia L., Egerton, Victoria M., and Manning, Phillip L. Morphological and chemical evidence for cyclic bone growth in a fossil hyaena. United States: N. p., 2018.
Web. doi:10.1039/c8ja00314a.
Anné, Jennifer, Wogelius, Roy A., Edwards, Nicholas P., van Veelen, Arjen, Buckley, Michael, Sellers, William I., Bergmann, Uwe, Sokaras, Dimosthenis, Alonso-Mori, Roberto, Harvey, Virginia L., Egerton, Victoria M., & Manning, Phillip L. Morphological and chemical evidence for cyclic bone growth in a fossil hyaena. United States. https://doi.org/10.1039/c8ja00314a
Anné, Jennifer, Wogelius, Roy A., Edwards, Nicholas P., van Veelen, Arjen, Buckley, Michael, Sellers, William I., Bergmann, Uwe, Sokaras, Dimosthenis, Alonso-Mori, Roberto, Harvey, Virginia L., Egerton, Victoria M., and Manning, Phillip L. Thu .
"Morphological and chemical evidence for cyclic bone growth in a fossil hyaena". United States. https://doi.org/10.1039/c8ja00314a. https://www.osti.gov/servlets/purl/1490883.
@article{osti_1490883,
title = {Morphological and chemical evidence for cyclic bone growth in a fossil hyaena},
author = {Anné, Jennifer and Wogelius, Roy A. and Edwards, Nicholas P. and van Veelen, Arjen and Buckley, Michael and Sellers, William I. and Bergmann, Uwe and Sokaras, Dimosthenis and Alonso-Mori, Roberto and Harvey, Virginia L. and Egerton, Victoria M. and Manning, Phillip L.},
abstractNote = {Trace element inventories are known to correlate with specific histological structures in bone, reflecting organismal physiology and life histories. By studying trace elements in fossilised bone, particularly in individuals with cyclic bone growth (alternating fast/slow bone deposition), we can improve our understanding of the physiology of extinct organisms. In this study we present the first direct comparison between optical histology (bone tissue identification) and synchrotron-based chemical mapping, quantification, and characterisation of trace elements (biochemistry) within cyclic growth tissues, in this case within bones of a cave hyaena (Crocuta crocuta spelaea). Results show distributions of zinc, an element strongly associated with active ossification and bone growth, correlating with (1) fast-growing tissue of zonal bone (cyclic growth) in an extinct hyaena and (2) secondary osteons (remodelling) in both extant and extinct hyaena. Concentrations and coordination chemistry of zinc within the fossil sample are comparable to those seen in extant bone suggesting that zinc is endogenous to the sample and that the chemistry of bone growth has been preserved for 40 ka. Furthermore, these results demonstrate that the study of trace elements as part of the histochemistry has wide utility for reconstructing growth, diet and other lifestyle factors in archaeological and fossil bone.},
doi = {10.1039/c8ja00314a},
journal = {Journal of Analytical Atomic Spectrometry},
number = 12,
volume = 33,
place = {United States},
year = {Thu Oct 18 00:00:00 EDT 2018},
month = {Thu Oct 18 00:00:00 EDT 2018}
}
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Figures / Tables:
Fig. 1: Optical histology of extant hyena. Optical histology under cross-polarized light of C. crocuta ribs in adult male (AMNH 187769, 83593) and female (AMNH 114226, 114227) specimens. All specimens can be divided into two tissue types: highly remodelled with secondary osteons ('2°') or woven tissues ('W'). Scale bar ismore »
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Figures / Tables found in this record:
Figures/Tables have been extracted from DOE-funded journal article accepted manuscripts.