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Title: Kinetic understanding of nitrogen supply condition on biosynthesis of polyhydroxyalkanoate from benzoate by Pseudomonas putida KT2440

Abstract

Nitrogen supply is critical to the synthesis of intracellular PHA in various bacteria. However, the specific role of the nitrogen in synthesizing PHA from benzoate, a lignin model compound use for the study of bacteria catabolism of aromatics, is still not clear. Here, two culture conditions were maintained for Pseudomonas putida KT2440 to produce PHA using benzoate as a carbon source. Under nitrogen-limited and surplus conditions, the accumulation of PHA was to 37.3% and 0.25% of cell dry weight, respectively. A model fit to the kinetics of biomass growth and PHA accumulation showed good agreement with data. GC–MS and NMR showed that PHA contained six hydroxyl fatty acid monomers under nitrogen-limited conditions, while two monomers were identified under nitrogen surplus conditions. The average molecular weight of PHA increased after the nitrogen source was exhausted. Lastly, these results provide a promising strategy for optimization of lignin to PHA yields.

Authors:
 [1];  [1];  [2];  [1];  [1];  [1];  [1]; ORCiD logo [3];  [4];  [1]
  1. Washington State Univ., Richland, WA (United States)
  2. Univ. of Tennessee, Knoxville, TN (United States)
  3. Univ. of Tennessee, Knoxville, TN (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
  4. Texas A & M Univ., College Station, TX (United States)
Publication Date:
Research Org.:
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
Sponsoring Org.:
USDOE Office of Energy Efficiency and Renewable Energy (EERE)
OSTI Identifier:
1489561
Alternate Identifier(s):
OSTI ID: 1636706
Grant/Contract Number:  
AC05-00OR22725; EE0007104; EE0006112
Resource Type:
Accepted Manuscript
Journal Name:
Bioresource Technology
Additional Journal Information:
Journal Volume: 273; Journal Issue: C; Journal ID: ISSN 0960-8524
Publisher:
Elsevier
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; Polyhydroxyalkanoates; Pseudomonas putida KT2440; Benzoate; Nitrogen; Kinetic modeling

Citation Formats

Xu, Zhangyang, Li, Xiaolu, Hao, Naijia, Pan, Chummei, de la torre, Luis, Ahamed, Aftab, Miller, John H., Ragauskas, Arthur J., Yuan, Joshua, and Yang, Bin. Kinetic understanding of nitrogen supply condition on biosynthesis of polyhydroxyalkanoate from benzoate by Pseudomonas putida KT2440. United States: N. p., 2018. Web. doi:10.1016/j.biortech.2018.11.046.
Xu, Zhangyang, Li, Xiaolu, Hao, Naijia, Pan, Chummei, de la torre, Luis, Ahamed, Aftab, Miller, John H., Ragauskas, Arthur J., Yuan, Joshua, & Yang, Bin. Kinetic understanding of nitrogen supply condition on biosynthesis of polyhydroxyalkanoate from benzoate by Pseudomonas putida KT2440. United States. https://doi.org/10.1016/j.biortech.2018.11.046
Xu, Zhangyang, Li, Xiaolu, Hao, Naijia, Pan, Chummei, de la torre, Luis, Ahamed, Aftab, Miller, John H., Ragauskas, Arthur J., Yuan, Joshua, and Yang, Bin. Mon . "Kinetic understanding of nitrogen supply condition on biosynthesis of polyhydroxyalkanoate from benzoate by Pseudomonas putida KT2440". United States. https://doi.org/10.1016/j.biortech.2018.11.046. https://www.osti.gov/servlets/purl/1489561.
@article{osti_1489561,
title = {Kinetic understanding of nitrogen supply condition on biosynthesis of polyhydroxyalkanoate from benzoate by Pseudomonas putida KT2440},
author = {Xu, Zhangyang and Li, Xiaolu and Hao, Naijia and Pan, Chummei and de la torre, Luis and Ahamed, Aftab and Miller, John H. and Ragauskas, Arthur J. and Yuan, Joshua and Yang, Bin},
abstractNote = {Nitrogen supply is critical to the synthesis of intracellular PHA in various bacteria. However, the specific role of the nitrogen in synthesizing PHA from benzoate, a lignin model compound use for the study of bacteria catabolism of aromatics, is still not clear. Here, two culture conditions were maintained for Pseudomonas putida KT2440 to produce PHA using benzoate as a carbon source. Under nitrogen-limited and surplus conditions, the accumulation of PHA was to 37.3% and 0.25% of cell dry weight, respectively. A model fit to the kinetics of biomass growth and PHA accumulation showed good agreement with data. GC–MS and NMR showed that PHA contained six hydroxyl fatty acid monomers under nitrogen-limited conditions, while two monomers were identified under nitrogen surplus conditions. The average molecular weight of PHA increased after the nitrogen source was exhausted. Lastly, these results provide a promising strategy for optimization of lignin to PHA yields.},
doi = {10.1016/j.biortech.2018.11.046},
journal = {Bioresource Technology},
number = C,
volume = 273,
place = {United States},
year = {2018},
month = {11}
}

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Cited by: 4 works
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Works referenced in this record:

Production of hydrocarbon compounds by endophytic fungi Gliocladium species grown on cellulose
journal, October 2011


Making lignin accessible for anaerobic digestion by wet-explosion pretreatment
journal, January 2015


Polyhydroxyalkanoates: Current applications in the medical field
journal, February 2016


Characterization of key triacylglycerol biosynthesis processes in rhodococci
journal, April 2016

  • Amara, Sawsan; Seghezzi, Nicolas; Otani, Hiroshi
  • Scientific Reports, Vol. 6, Issue 1
  • DOI: 10.1038/srep24985

Dual role of lignin in plant litter decomposition in terrestrial ecosystems
journal, February 2010

  • Austin, A. T.; Ballare, C. L.
  • Proceedings of the National Academy of Sciences, Vol. 107, Issue 10
  • DOI: 10.1073/pnas.0909396107

PHA Productivity and Yield of Ralstonia eutropha When Intermittently or Continuously Fed a Mixture of Short Chain Fatty Acids
journal, January 2012

  • Chakraborty, Panchali; Muthukumarappan, Kasiviswanathan; Gibbons, William R.
  • Journal of Biomedicine and Biotechnology, Vol. 2012
  • DOI: 10.1155/2012/506153

Production of Medium-Chain-Length Polyhydroxyalkanoates by Pseudomonas aeruginosa With Fatty Acids and Alternative Carbon Sources
journal, January 2006

  • Chan, Pui-Ling; Yu, Vincent; Wai, Lam
  • Applied Biochemistry and Biotechnology, Vol. 132, Issue 1-3
  • DOI: 10.1385/ABAB:132:1:933

Heteronuclear NMR analysis of unsaturated fatty acids in poly(3-hydroxyalkanoates). Study of beta-oxidation in Pseudomonas putida.
journal, January 1993


Analysis of the time course of degradation for fully biobased nonwoven agricultural mulches in compost-enriched soil
journal, November 2015

  • Dharmalingam, Sathiskumar; Hayes, Douglas G.; Wadsworth, Larry C.
  • Textile Research Journal, Vol. 86, Issue 13
  • DOI: 10.1177/0040517515612358

Modeling of single-cell oil production under nitrogen-limited and substrate inhibition conditions
journal, December 2010

  • Economou, C. N.; Aggelis, G.; Pavlou, S.
  • Biotechnology and Bioengineering, Vol. 108, Issue 5
  • DOI: 10.1002/bit.23026

Enhancement of Stress Tolerance in the Polyhydroxyalkanoate Producers without Mobilization of the Accumulated Granules
journal, November 2013

  • Goh, Lay-Koon; Purama, Ravi Kiran; Sudesh, Kumar
  • Applied Biochemistry and Biotechnology, Vol. 172, Issue 3
  • DOI: 10.1007/s12010-013-0634-z

Biosynthesis and Characterization of Polyhydroxyalkanoates Copolymers Produced by Pseudomonas putida Bet001 Isolated from Palm Oil Mill Effluent
journal, September 2012


NtrC-Dependent Regulatory Network for Nitrogen Assimilation in Pseudomonas putida
journal, July 2009

  • Hervás, Ana B.; Canosa, Inés; Little, Richard
  • Journal of Bacteriology, Vol. 191, Issue 19
  • DOI: 10.1128/JB.00744-09

Compatibilization in bio-based and biodegradable polymer blends
journal, June 2013


Metabolomics-based component profiling of Halomonas sp. KM-1 during different growth phases in poly(3-hydroxybutyrate) production
journal, July 2013


Recent Advances and Challenges towards Sustainable Polyhydroxyalkanoate (PHA) Production
journal, June 2017


Genomic and proteomic analysis of lignin degrading and polyhydroxyalkanoate accumulating β-proteobacterium Pandoraea sp. ISTKB
journal, June 2018

  • Kumar, Madan; Verma, Sandhya; Gazara, Rajesh Kumar
  • Biotechnology for Biofuels, Vol. 11, Issue 1
  • DOI: 10.1186/s13068-018-1148-2

Use of thiol-ene click chemistry to modify mechanical and thermal properties of polyhydroxyalkanoates (PHAs)
journal, February 2016

  • Levine, Alex C.; Heberlig, Graham W.; Nomura, Christopher T.
  • International Journal of Biological Macromolecules, Vol. 83
  • DOI: 10.1016/j.ijbiomac.2015.11.048

Systems biology-guided biodesign of consolidated lignin conversion
journal, January 2016

  • Lin, Lu; Cheng, Yanbing; Pu, Yunqiao
  • Green Chemistry, Vol. 18, Issue 20
  • DOI: 10.1039/C6GC01131D

Lignin valorization through integrated biological funneling and chemical catalysis
journal, August 2014

  • Linger, J. G.; Vardon, D. R.; Guarnieri, M. T.
  • Proceedings of the National Academy of Sciences, Vol. 111, Issue 33, p. 12013-12018
  • DOI: 10.1073/pnas.1410657111

Synergistic maximization of the carbohydrate output and lignin processability by combinatorial pretreatment
journal, January 2017

  • Liu, Zhi-Hua; Olson, Michelle L.; Shinde, Somnath
  • Green Chem., Vol. 19, Issue 20
  • DOI: 10.1039/C7GC02057K

Medium chain length polyhydroxyalkanoates consisting primarily of unsaturated 3-hydroxy-5-cis-dodecanoate synthesized by newly isolated bacteria using crude glycerol
journal, March 2016

  • Muangwong, Amtiga; Boontip, Thanawat; Pachimsawat, Jittakan
  • Microbial Cell Factories, Vol. 15, Issue 1
  • DOI: 10.1186/s12934-016-0454-2

A genome-scale metabolic reconstruction of Pseudomonas putida KT2440: iJN746 as a cell factory
journal, January 2008

  • Nogales, Juan; Palsson, Bernhard Ø; Thiele, Ines
  • BMC Systems Biology, Vol. 2, Issue 1
  • DOI: 10.1186/1752-0509-2-79

Towards lignin consolidated bioprocessing: simultaneous lignin depolymerization and product generation by bacteria
journal, January 2015

  • Salvachúa, Davinia; Karp, Eric M.; Nimlos, Claire T.
  • Green Chemistry, Vol. 17, Issue 11
  • DOI: 10.1039/C5GC01165E

Impact of carbon source and variable nitrogen conditions on bacterial biosynthesis of polyhydroxyalkanoates: Evidence of an atypical metabolism in Bacillus megaterium DSM 509
journal, September 2013

  • Shahid, Salma; Mosrati, Ridha; Ledauphin, Jérôme
  • Journal of Bioscience and Bioengineering, Vol. 116, Issue 3
  • DOI: 10.1016/j.jbiosc.2013.02.017

Process optimization for efficient biomediated PHA production from animal-based waste streams
journal, February 2012

  • Titz, Michaela; Kettl, Karl-Heinz; Shahzad, Khurram
  • Clean Technologies and Environmental Policy, Vol. 14, Issue 3
  • DOI: 10.1007/s10098-012-0464-7

Understanding the Limitations in the Biosynthesis of Polyhydroxyalkanoate (PHA) from Lignin Derivatives
journal, March 2014

  • Tomizawa, Satoshi; Chuah, Jo-Ann; Matsumoto, Keiji
  • ACS Sustainable Chemistry & Engineering, Vol. 2, Issue 5
  • DOI: 10.1021/sc500066f

The impact of succinate trace on pWW0 and ortho -cleavage pathway transcription in Pseudomonas putida mt-2 during toluene biodegradation
journal, June 2017


cis,cis-Muconic acid: separation and catalysis to bio-adipic acid for nylon-6,6 polymerization
journal, January 2016

  • Vardon, Derek R.; Rorrer, Nicholas A.; Salvachúa, Davinia
  • Green Chemistry, Vol. 18, Issue 11
  • DOI: 10.1039/C5GC02844B

Simultaneous Improvements of Pseudomonas Cell Growth and Polyhydroxyalkanoate Production from a Lignin Derivative for Lignin-Consolidated Bioprocessing
journal, July 2018

  • Wang, Xiaopeng; Lin, Lu; Dong, Junde
  • Applied and Environmental Microbiology, Vol. 84, Issue 18
  • DOI: 10.1128/AEM.01469-18

Lignin depolymerization and utilization by bacteria
journal, December 2018


NtrC-sensed nitrogen availability is important for oxidative stress defense in Pseudomonas putida KT2440
journal, April 2010


Works referencing / citing this record:

Production of polyhydroxyalkanoates by halotolerant bacteria with volatile fatty acids from food waste as carbon source
journal, November 2019

  • Wang, Pan; Chen, Xi Teng; Qiu, Yin Quan
  • Biotechnology and Applied Biochemistry
  • DOI: 10.1002/bab.1848

Discovery of potential pathways for biological conversion of poplar wood into lipids by co-fermentation of Rhodococci strains
journal, March 2019


Bioconversion of lignin and its derivatives into polyhydroxyalkanoates: Challenges and opportunities
journal, December 2018

  • Kumar, Prasun; Maharjan, Anoth; Jun, Hang‐Bae
  • Biotechnology and Applied Biochemistry, Vol. 66, Issue 2
  • DOI: 10.1002/bab.1720

Recent advances in lignin valorization with bacterial cultures: microorganisms, metabolic pathways, and bio-products
journal, February 2019