Kinetic understanding of nitrogen supply condition on biosynthesis of polyhydroxyalkanoate from benzoate by Pseudomonas putida KT2440
Abstract
Nitrogen supply is critical to the synthesis of intracellular PHA in various bacteria. However, the specific role of the nitrogen in synthesizing PHA from benzoate, a lignin model compound use for the study of bacteria catabolism of aromatics, is still not clear. Here, two culture conditions were maintained for Pseudomonas putida KT2440 to produce PHA using benzoate as a carbon source. Under nitrogen-limited and surplus conditions, the accumulation of PHA was to 37.3% and 0.25% of cell dry weight, respectively. A model fit to the kinetics of biomass growth and PHA accumulation showed good agreement with data. GC–MS and NMR showed that PHA contained six hydroxyl fatty acid monomers under nitrogen-limited conditions, while two monomers were identified under nitrogen surplus conditions. The average molecular weight of PHA increased after the nitrogen source was exhausted. Lastly, these results provide a promising strategy for optimization of lignin to PHA yields.
- Authors:
-
[3];
- Washington State Univ., Richland, WA (United States)
- Univ. of Tennessee, Knoxville, TN (United States)
- Univ. of Tennessee, Knoxville, TN (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Texas A & M Univ., College Station, TX (United States)
- Publication Date:
- Research Org.:
- Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
- Sponsoring Org.:
- USDOE Office of Energy Efficiency and Renewable Energy (EERE)
- OSTI Identifier:
- 1489561
- Alternate Identifier(s):
- OSTI ID: 1636706
- Grant/Contract Number:
- AC05-00OR22725; EE0007104; EE0006112
- Resource Type:
- Accepted Manuscript
- Journal Name:
- Bioresource Technology
- Additional Journal Information:
- Journal Volume: 273; Journal Issue: C; Journal ID: ISSN 0960-8524
- Publisher:
- Elsevier
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; Polyhydroxyalkanoates; Pseudomonas putida KT2440; Benzoate; Nitrogen; Kinetic modeling
Citation Formats
Xu, Zhangyang, Li, Xiaolu, Hao, Naijia, Pan, Chummei, de la torre, Luis, Ahamed, Aftab, Miller, John H., Ragauskas, Arthur J., Yuan, Joshua, and Yang, Bin. Kinetic understanding of nitrogen supply condition on biosynthesis of polyhydroxyalkanoate from benzoate by Pseudomonas putida KT2440. United States: N. p., 2018.
Web. doi:10.1016/j.biortech.2018.11.046.
Xu, Zhangyang, Li, Xiaolu, Hao, Naijia, Pan, Chummei, de la torre, Luis, Ahamed, Aftab, Miller, John H., Ragauskas, Arthur J., Yuan, Joshua, & Yang, Bin. Kinetic understanding of nitrogen supply condition on biosynthesis of polyhydroxyalkanoate from benzoate by Pseudomonas putida KT2440. United States. https://doi.org/10.1016/j.biortech.2018.11.046
Xu, Zhangyang, Li, Xiaolu, Hao, Naijia, Pan, Chummei, de la torre, Luis, Ahamed, Aftab, Miller, John H., Ragauskas, Arthur J., Yuan, Joshua, and Yang, Bin. Mon .
"Kinetic understanding of nitrogen supply condition on biosynthesis of polyhydroxyalkanoate from benzoate by Pseudomonas putida KT2440". United States. https://doi.org/10.1016/j.biortech.2018.11.046. https://www.osti.gov/servlets/purl/1489561.
@article{osti_1489561,
title = {Kinetic understanding of nitrogen supply condition on biosynthesis of polyhydroxyalkanoate from benzoate by Pseudomonas putida KT2440},
author = {Xu, Zhangyang and Li, Xiaolu and Hao, Naijia and Pan, Chummei and de la torre, Luis and Ahamed, Aftab and Miller, John H. and Ragauskas, Arthur J. and Yuan, Joshua and Yang, Bin},
abstractNote = {Nitrogen supply is critical to the synthesis of intracellular PHA in various bacteria. However, the specific role of the nitrogen in synthesizing PHA from benzoate, a lignin model compound use for the study of bacteria catabolism of aromatics, is still not clear. Here, two culture conditions were maintained for Pseudomonas putida KT2440 to produce PHA using benzoate as a carbon source. Under nitrogen-limited and surplus conditions, the accumulation of PHA was to 37.3% and 0.25% of cell dry weight, respectively. A model fit to the kinetics of biomass growth and PHA accumulation showed good agreement with data. GC–MS and NMR showed that PHA contained six hydroxyl fatty acid monomers under nitrogen-limited conditions, while two monomers were identified under nitrogen surplus conditions. The average molecular weight of PHA increased after the nitrogen source was exhausted. Lastly, these results provide a promising strategy for optimization of lignin to PHA yields.},
doi = {10.1016/j.biortech.2018.11.046},
journal = {Bioresource Technology},
number = C,
volume = 273,
place = {United States},
year = {Mon Nov 12 00:00:00 EST 2018},
month = {Mon Nov 12 00:00:00 EST 2018}
}
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Works referenced in this record:
Production of hydrocarbon compounds by endophytic fungi Gliocladium species grown on cellulose
journal, October 2011
- Ahamed, Aftab; Ahring, Birgitte K.
- Bioresource Technology, Vol. 102, Issue 20
Making lignin accessible for anaerobic digestion by wet-explosion pretreatment
journal, January 2015
- Ahring, Birgitte K.; Biswas, Rajib; Ahamed, Aftab
- Bioresource Technology, Vol. 175
Polyhydroxyalkanoates: Current applications in the medical field
journal, February 2016
- Ali, Iftikhar; Jamil, Nazia
- Frontiers in Biology, Vol. 11, Issue 1
Characterization of key triacylglycerol biosynthesis processes in rhodococci
journal, April 2016
- Amara, Sawsan; Seghezzi, Nicolas; Otani, Hiroshi
- Scientific Reports, Vol. 6, Issue 1
Dual role of lignin in plant litter decomposition in terrestrial ecosystems
journal, February 2010
- Austin, A. T.; Ballare, C. L.
- Proceedings of the National Academy of Sciences, Vol. 107, Issue 10
Enhanced production of medium-chain-length polyhydroxyalkanoates (PHA) by PHA depolymerase knockout mutant of Pseudomonas putida KT2442
journal, April 2009
- Cai, L.; Yuan, M.; Liu, F.
- Bioresource Technology, Vol. 100, Issue 7
PHA Productivity and Yield of Ralstonia eutropha When Intermittently or Continuously Fed a Mixture of Short Chain Fatty Acids
journal, January 2012
- Chakraborty, Panchali; Muthukumarappan, Kasiviswanathan; Gibbons, William R.
- Journal of Biomedicine and Biotechnology, Vol. 2012
Production of Medium-Chain-Length Polyhydroxyalkanoates by Pseudomonas aeruginosa With Fatty Acids and Alternative Carbon Sources
journal, January 2006
- Chan, Pui-Ling; Yu, Vincent; Wai, Lam
- Applied Biochemistry and Biotechnology, Vol. 132, Issue 1-3
Exploiting metagenomic diversity for novel polyhydroxyalkanoate synthases: Production of a terpolymer poly(3-hydroxybutyrate-co-3-hydroxyhexanoate-co-3-hydroxyoctanoate) with a recombinant Pseudomonas putida strain
journal, January 2012
- Cheema, Simrita; Bassas-Galia, Monica; Sarma, Priyangshu M.
- Bioresource Technology, Vol. 103, Issue 1
Heteronuclear NMR analysis of unsaturated fatty acids in poly(3-hydroxyalkanoates). Study of beta-oxidation in Pseudomonas putida.
journal, January 1993
- de Waard, P.; van der Wal, H.; Huijberts, G. N.
- Journal of Biological Chemistry, Vol. 268, Issue 1
Analysis of the time course of degradation for fully biobased nonwoven agricultural mulches in compost-enriched soil
journal, November 2015
- Dharmalingam, Sathiskumar; Hayes, Douglas G.; Wadsworth, Larry C.
- Textile Research Journal, Vol. 86, Issue 13
Modeling of single-cell oil production under nitrogen-limited and substrate inhibition conditions
journal, December 2010
- Economou, C. N.; Aggelis, G.; Pavlou, S.
- Biotechnology and Bioengineering, Vol. 108, Issue 5
Quantitative ‘Omics Analyses of Medium Chain Length Polyhydroxyalkanaote Metabolism in Pseudomonas putida LS46 Cultured with Waste Glycerol and Waste Fatty Acids
journal, November 2015
- Fu, Jilagamazhi; Sharma, Parveen; Spicer, Vic
- PLOS ONE, Vol. 10, Issue 11
Enhancement of Stress Tolerance in the Polyhydroxyalkanoate Producers without Mobilization of the Accumulated Granules
journal, November 2013
- Goh, Lay-Koon; Purama, Ravi Kiran; Sudesh, Kumar
- Applied Biochemistry and Biotechnology, Vol. 172, Issue 3
Biosynthesis and Characterization of Polyhydroxyalkanoates Copolymers Produced by Pseudomonas putida Bet001 Isolated from Palm Oil Mill Effluent
journal, September 2012
- Gumel, Ahmad Mohammed; Annuar, Mohamad Suffian Mohamad; Heidelberg, Thorsten
- PLoS ONE, Vol. 7, Issue 9
Polyhydroxyalkanoate (PHA) Accumulation in Sulfate-Reducing Bacteria and Identification of a Class III PHA Synthase (PhaEC) in Desulfococcus multivorans
journal, August 2004
- Hai, T.; Lange, D.; Rabus, R.
- Applied and Environmental Microbiology, Vol. 70, Issue 8
NtrC-Dependent Regulatory Network for Nitrogen Assimilation in Pseudomonas putida
journal, July 2009
- Hervás, Ana B.; Canosa, Inés; Little, Richard
- Journal of Bacteriology, Vol. 191, Issue 19
Compatibilization in bio-based and biodegradable polymer blends
journal, June 2013
- Imre, B.; Pukánszky, B.
- European Polymer Journal, Vol. 49, Issue 6
Metabolomics-based component profiling of Halomonas sp. KM-1 during different growth phases in poly(3-hydroxybutyrate) production
journal, July 2013
- Jin, You-Xun; Shi, Lian-Hua; Kawata, Yoshikazu
- Bioresource Technology, Vol. 140
Recent Advances and Challenges towards Sustainable Polyhydroxyalkanoate (PHA) Production
journal, June 2017
- Kourmentza, Constantina; Plácido, Jersson; Venetsaneas, Nikolaos
- Bioengineering, Vol. 4, Issue 4
Burkholderia thailandensis as a microbial cell factory for the bioconversion of used cooking oil to polyhydroxyalkanoates and rhamnolipids
journal, January 2018
- Kourmentza, C.; Costa, J.; Azevedo, Z.
- Bioresource Technology, Vol. 247
Genomic and proteomic analysis of lignin degrading and polyhydroxyalkanoate accumulating β-proteobacterium Pandoraea sp. ISTKB
journal, June 2018
- Kumar, Madan; Verma, Sandhya; Gazara, Rajesh Kumar
- Biotechnology for Biofuels, Vol. 11, Issue 1
Use of thiol-ene click chemistry to modify mechanical and thermal properties of polyhydroxyalkanoates (PHAs)
journal, February 2016
- Levine, Alex C.; Heberlig, Graham W.; Nomura, Christopher T.
- International Journal of Biological Macromolecules, Vol. 83
Systems biology-guided biodesign of consolidated lignin conversion
journal, January 2016
- Lin, Lu; Cheng, Yanbing; Pu, Yunqiao
- Green Chemistry, Vol. 18, Issue 20
Lignin valorization through integrated biological funneling and chemical catalysis
journal, August 2014
- Linger, J. G.; Vardon, D. R.; Guarnieri, M. T.
- Proceedings of the National Academy of Sciences, Vol. 111, Issue 33, p. 12013-12018
Synergistic maximization of the carbohydrate output and lignin processability by combinatorial pretreatment
journal, January 2017
- Liu, Zhi-Hua; Olson, Michelle L.; Shinde, Somnath
- Green Chem., Vol. 19, Issue 20
Medium chain length polyhydroxyalkanoates consisting primarily of unsaturated 3-hydroxy-5-cis-dodecanoate synthesized by newly isolated bacteria using crude glycerol
journal, March 2016
- Muangwong, Amtiga; Boontip, Thanawat; Pachimsawat, Jittakan
- Microbial Cell Factories, Vol. 15, Issue 1
A genome-scale metabolic reconstruction of Pseudomonas putida KT2440: iJN746 as a cell factory
journal, January 2008
- Nogales, Juan; Palsson, Bernhard Ø; Thiele, Ines
- BMC Systems Biology, Vol. 2, Issue 1
Towards lignin consolidated bioprocessing: simultaneous lignin depolymerization and product generation by bacteria
journal, January 2015
- Salvachúa, Davinia; Karp, Eric M.; Nimlos, Claire T.
- Green Chemistry, Vol. 17, Issue 11
Impact of carbon source and variable nitrogen conditions on bacterial biosynthesis of polyhydroxyalkanoates: Evidence of an atypical metabolism in Bacillus megaterium DSM 509
journal, September 2013
- Shahid, Salma; Mosrati, Ridha; Ledauphin, Jérôme
- Journal of Bioscience and Bioengineering, Vol. 116, Issue 3
Production and characterization of homopolymer poly(3-hydroxyvalerate) (PHV) accumulated by wild type and recombinant Aeromonas hydrophila strain 4AK4
journal, September 2009
- Shen, Xiao-Wen; Yang, Yun; Jian, Jia
- Bioresource Technology, Vol. 100, Issue 18
Process optimization for efficient biomediated PHA production from animal-based waste streams
journal, February 2012
- Titz, Michaela; Kettl, Karl-Heinz; Shahzad, Khurram
- Clean Technologies and Environmental Policy, Vol. 14, Issue 3
Understanding the Limitations in the Biosynthesis of Polyhydroxyalkanoate (PHA) from Lignin Derivatives
journal, March 2014
- Tomizawa, Satoshi; Chuah, Jo-Ann; Matsumoto, Keiji
- ACS Sustainable Chemistry & Engineering, Vol. 2, Issue 5
The impact of succinate trace on pWW0 and ortho -cleavage pathway transcription in Pseudomonas putida mt-2 during toluene biodegradation
journal, June 2017
- Tsipa, Argyro; Koutinas, Michalis; Vernardis, Spyros I.
- Bioresource Technology, Vol. 234
cis,cis-Muconic acid: separation and catalysis to bio-adipic acid for nylon-6,6 polymerization
journal, January 2016
- Vardon, Derek R.; Rorrer, Nicholas A.; Salvachúa, Davinia
- Green Chemistry, Vol. 18, Issue 11
Simultaneous Improvements of Pseudomonas Cell Growth and Polyhydroxyalkanoate Production from a Lignin Derivative for Lignin-Consolidated Bioprocessing
journal, July 2018
- Wang, Xiaopeng; Lin, Lu; Dong, Junde
- Applied and Environmental Microbiology, Vol. 84, Issue 18
Biosynthesis and characterization of novel poly(3-hydroxybutyrate-co-3-hydroxy-2-methylbutyrate): thermal behavior associated with α-carbon methylation
journal, January 2015
- Watanabe, Yoriko; Ishizuka, Koya; Furutate, Sho
- RSC Advances, Vol. 5, Issue 72
Lignin depolymerization and utilization by bacteria
journal, December 2018
- Xu, Rong; Zhang, Kai; Liu, Pu
- Bioresource Technology, Vol. 269
NtrC-sensed nitrogen availability is important for oxidative stress defense in Pseudomonas putida KT2440
journal, April 2010
- Yeom, Sujin; Yeom, Jinki; Park, Woojun
- The Journal of Microbiology, Vol. 48, Issue 2
Works referencing / citing this record:
Production of polyhydroxyalkanoates by halotolerant bacteria with volatile fatty acids from food waste as carbon source
journal, November 2019
- Wang, Pan; Chen, Xi Teng; Qiu, Yin Quan
- Biotechnology and Applied Biochemistry
Discovery of potential pathways for biological conversion of poplar wood into lipids by co-fermentation of Rhodococci strains
journal, March 2019
- Li, Xiaolu; He, Yucai; Zhang, Libing
- Biotechnology for Biofuels, Vol. 12, Issue 1
Bioconversion of lignin and its derivatives into polyhydroxyalkanoates: Challenges and opportunities
journal, December 2018
- Kumar, Prasun; Maharjan, Anoth; Jun, Hang‐Bae
- Biotechnology and Applied Biochemistry, Vol. 66, Issue 2
Recent advances in lignin valorization with bacterial cultures: microorganisms, metabolic pathways, and bio-products
journal, February 2019
- Xu, Zhaoxian; Lei, Peng; Zhai, Rui
- Biotechnology for Biofuels, Vol. 12, Issue 1