pH selectively regulates citric acid and lipid production in Yarrowia lipolytica W29 during nitrogen-limited growth on glucose

doi:10.1016/j.jbiotec.2018.10.012

Title: pH selectively regulates citric acid and lipid production in Yarrowia lipolytica W29 during nitrogen-limited growth on glucose

Abstract

Yarrowia lipolytica has been used to produce both citric acid and lipid-based bioproducts at high titers. In this study, we found that pH differentially affects citric acid and lipid production in Y. lipolytica W29, with citric acid production enhanced at more neutral pH’s and lipid production enhanced at more acid pH’s. To determine the mechanism governing this pH-dependent switch between citric acid and lipid production, we profiled gene expression at different pH’s and found that the relative expression of multiple transporters is increased at neutral pH. These results suggest that this pH-dependent switch is mediated at the level of citric acid transport rather than changes in the expression of the enzymes involved in citric acid and lipid metabolism. In further support of this mechanism, thermodynamic calculations suggest that citric acid secretion is more energetically favorable at neutral pH’s, assuming the fully protonated acid is the substrate for secretion. Finally, collectively, these results provide new insights regarding citric acid and lipid production in Y. lipolytica and may offer new strategies for metabolic engineering and process design.

Authors:

Zhang, Shuyan ^[1]; Jagtap, Sujit Sadashiv ^[1]; Deewan, Anshu ^[1]; Rao, Christopher V. ^[1]

Univ. of Illinois, Urbana-Champaign, IL (United States). DOE Center for Advanced Bioenergy and Bioproducts Innovation, Carl R. Woese Inst. of Genomic Biology, Dept. of Chemical and Biomolecular Engineering

Publication Date:: 2018-11-26

Research Org.:: Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), Urbana, IL (United States)

Sponsoring Org.:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

OSTI Identifier:: 1484862

Grant/Contract Number:: SC0018420

Resource Type:: Accepted Manuscript

Journal Name:: Journal of Biotechnology

Additional Journal Information:: Journal Volume: 290; Journal ID: ISSN 0168-1656

Publisher:: Elsevier

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; Yarrowia lipolytica; citric acid; oleaginous yeast; lipids; transporter; RNAseq

Citation Formats


                    Zhang, Shuyan, Jagtap, Sujit Sadashiv, Deewan, Anshu, and Rao, Christopher V. pH selectively regulates citric acid and lipid production in Yarrowia lipolytica W29 during nitrogen-limited growth on glucose.  United States: N. p., 2018. 
        Web.  doi:10.1016/j.jbiotec.2018.10.012.

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                    Zhang, Shuyan, Jagtap, Sujit Sadashiv, Deewan, Anshu, & Rao, Christopher V. pH selectively regulates citric acid and lipid production in Yarrowia lipolytica W29 during nitrogen-limited growth on glucose.  United States.  doi:10.1016/j.jbiotec.2018.10.012.

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                    Zhang, Shuyan, Jagtap, Sujit Sadashiv, Deewan, Anshu, and Rao, Christopher V. Mon .  
        "pH selectively regulates citric acid and lipid production in Yarrowia lipolytica W29 during nitrogen-limited growth on glucose".  United States.  doi:10.1016/j.jbiotec.2018.10.012.  https://www.osti.gov/servlets/purl/1484862.

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@article{osti_1484862,

  title        = {pH selectively regulates citric acid and lipid production in Yarrowia lipolytica W29 during nitrogen-limited growth on glucose},

  author       = {Zhang, Shuyan and Jagtap, Sujit Sadashiv and Deewan, Anshu and Rao, Christopher V.},

  abstractNote = {Yarrowia lipolytica has been used to produce both citric acid and lipid-based bioproducts at high titers. In this study, we found that pH differentially affects citric acid and lipid production in Y. lipolytica W29, with citric acid production enhanced at more neutral pH’s and lipid production enhanced at more acid pH’s. To determine the mechanism governing this pH-dependent switch between citric acid and lipid production, we profiled gene expression at different pH’s and found that the relative expression of multiple transporters is increased at neutral pH. These results suggest that this pH-dependent switch is mediated at the level of citric acid transport rather than changes in the expression of the enzymes involved in citric acid and lipid metabolism. In further support of this mechanism, thermodynamic calculations suggest that citric acid secretion is more energetically favorable at neutral pH’s, assuming the fully protonated acid is the substrate for secretion. Finally, collectively, these results provide new insights regarding citric acid and lipid production in Y. lipolytica and may offer new strategies for metabolic engineering and process design.},

  doi          = {10.1016/j.jbiotec.2018.10.012},

  journal      = {Journal of Biotechnology},

  number       = ,

  volume       = 290,

  place        = {United States},

  year         = {2018},

  month        = {11}

}

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DOI: 10.1016/j.jbiotec.2018.10.012

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Works referencing / citing this record:

Multi-Omics Analysis of Fatty Alcohol Production in Engineered Yeasts Saccharomyces cerevisiae and Yarrowia lipolytica
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Dahlin, Jonathan; Holkenbrink, Carina; Marella, Eko Roy
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Multi-Omics Analysis of Fatty Alcohol Production in Engineered Yeasts Saccharomyces cerevisiae and Yarrowia lipolytica
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Dahlin, Jonathan; Holkenbrink, Carina; Marella, Eko Roy
Frontiers in Genetics, Vol. 10
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Microbial production of (2 R ,3 S )-isocitric acid: state of the arts and prospects
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Kamzolova, Svetlana V.; Morgunov, Igor G.
Applied Microbiology and Biotechnology, Vol. 103, Issue 23-24
DOI: 10.1007/s00253-019-10207-4

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Title: pH selectively regulates citric acid and lipid production in Yarrowia lipolytica W29 during nitrogen-limited growth on glucose

Abstract

Citation Formats

Multi-Omics Analysis of Fatty Alcohol Production in Engineered Yeasts Saccharomyces cerevisiae and Yarrowia lipolytica journal, August 2019

Multi-Omics Analysis of Fatty Alcohol Production in Engineered Yeasts Saccharomyces cerevisiae and Yarrowia lipolytica journal, August 2019

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Multi-Omics Analysis of Fatty Alcohol Production in Engineered Yeasts Saccharomyces cerevisiae and Yarrowia lipolytica
journal, August 2019

Multi-Omics Analysis of Fatty Alcohol Production in Engineered Yeasts Saccharomyces cerevisiae and Yarrowia lipolytica
journal, August 2019

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