pH selectively regulates citric acid and lipid production in Yarrowia lipolytica W29 during nitrogen-limited growth on glucose

doi:10.1016/j.jbiotec.2018.10.012

Title: pH selectively regulates citric acid and lipid production in Yarrowia lipolytica W29 during nitrogen-limited growth on glucose

Full Record
Other Related Research

Abstract

Yarrowia lipolytica has been used to produce both citric acid and lipid-based bioproducts at high titers. In this study, we found that pH differentially affects citric acid and lipid production in Y. lipolytica W29, with citric acid production enhanced at more neutral pH’s and lipid production enhanced at more acid pH’s. To determine the mechanism governing this pH-dependent switch between citric acid and lipid production, we profiled gene expression at different pH’s and found that the relative expression of multiple transporters is increased at neutral pH. These results suggest that this pH-dependent switch is mediated at the level of citric acid transport rather than changes in the expression of the enzymes involved in citric acid and lipid metabolism. In further support of this mechanism, thermodynamic calculations suggest that citric acid secretion is more energetically favorable at neutral pH’s, assuming the fully protonated acid is the substrate for secretion. Finally, collectively, these results provide new insights regarding citric acid and lipid production in Y. lipolytica and may offer new strategies for metabolic engineering and process design.

Authors:

Zhang, Shuyan ^[1]; Jagtap, Sujit Sadashiv ^[1]; Deewan, Anshu ^[1]; Rao, Christopher V. ^[1]

Univ. of Illinois, Urbana-Champaign, IL (United States). DOE Center for Advanced Bioenergy and Bioproducts Innovation, Carl R. Woese Inst. of Genomic Biology, Dept. of Chemical and Biomolecular Engineering

Publication Date:: 2018-11-26

Research Org.:: Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), Urbana, IL (United States)

Sponsoring Org.:: USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)

OSTI Identifier:: 1484862

Grant/Contract Number:: SC0018420

Resource Type:: Accepted Manuscript

Journal Name:: Journal of Biotechnology

Additional Journal Information:: Journal Volume: 290; Journal ID: ISSN 0168-1656

Publisher:: Elsevier

Country of Publication:: United States

Language:: English

Subject:: 59 BASIC BIOLOGICAL SCIENCES; Yarrowia lipolytica; citric acid; oleaginous yeast; lipids; transporter; RNAseq

Citation Formats


                    Zhang, Shuyan, Jagtap, Sujit Sadashiv, Deewan, Anshu, and Rao, Christopher V. pH selectively regulates citric acid and lipid production in Yarrowia lipolytica W29 during nitrogen-limited growth on glucose.  United States: N. p., 2018. 
        Web.  doi:10.1016/j.jbiotec.2018.10.012.

Copy to clipboard


                    Zhang, Shuyan, Jagtap, Sujit Sadashiv, Deewan, Anshu, & Rao, Christopher V. pH selectively regulates citric acid and lipid production in Yarrowia lipolytica W29 during nitrogen-limited growth on glucose.  United States.  doi:10.1016/j.jbiotec.2018.10.012.

Copy to clipboard


                    Zhang, Shuyan, Jagtap, Sujit Sadashiv, Deewan, Anshu, and Rao, Christopher V. Mon .  
        "pH selectively regulates citric acid and lipid production in Yarrowia lipolytica W29 during nitrogen-limited growth on glucose".  United States.  doi:10.1016/j.jbiotec.2018.10.012.  https://www.osti.gov/servlets/purl/1484862.

Copy to clipboard


                    
@article{osti_1484862,

  title        = {pH selectively regulates citric acid and lipid production in Yarrowia lipolytica W29 during nitrogen-limited growth on glucose},

  author       = {Zhang, Shuyan and Jagtap, Sujit Sadashiv and Deewan, Anshu and Rao, Christopher V.},

  abstractNote = {Yarrowia lipolytica has been used to produce both citric acid and lipid-based bioproducts at high titers. In this study, we found that pH differentially affects citric acid and lipid production in Y. lipolytica W29, with citric acid production enhanced at more neutral pH’s and lipid production enhanced at more acid pH’s. To determine the mechanism governing this pH-dependent switch between citric acid and lipid production, we profiled gene expression at different pH’s and found that the relative expression of multiple transporters is increased at neutral pH. These results suggest that this pH-dependent switch is mediated at the level of citric acid transport rather than changes in the expression of the enzymes involved in citric acid and lipid metabolism. In further support of this mechanism, thermodynamic calculations suggest that citric acid secretion is more energetically favorable at neutral pH’s, assuming the fully protonated acid is the substrate for secretion. Finally, collectively, these results provide new insights regarding citric acid and lipid production in Y. lipolytica and may offer new strategies for metabolic engineering and process design.},

  doi          = {10.1016/j.jbiotec.2018.10.012},

  journal      = {Journal of Biotechnology},

  number       = ,

  volume       = 290,

  place        = {United States},

  year         = {2018},

  month        = {11}

}

Copy to clipboard

Journal Article:

Free Publicly Available Full Text

Accepted Manuscript (DOE)

Publisher's Version of Record

DOI: 10.1016/j.jbiotec.2018.10.012

Other availability

Search WorldCat to find libraries that may hold this journal

Citation Metrics:

Cited by: 2 works

Citation information provided by
Web of Science

Save / Share:

Export Metadata

Save to My Library

Similar records in OSTI.GOV collections:

Ameliorating the Metabolic Burden of the Co-expression of Secreted Fungal Cellulases in a High Lipid-Accumulating Yarrowia lipolytica Strain by Medium C/N Ratio and a Chemical Chaperone

Journal Article Wei, Hui ; Wang, Wei ; Alper, Hal S. ; ... - Frontiers in Microbiology

Yarrowia lipolytica, known to accumulate lipids intracellularly, lacks the cellulolytic enzymes needed to break down solid biomass directly. This study aimed to evaluate the potential metabolic burden of expressing core cellulolytic enzymes in an engineered high lipid-accumulating strain of Y. lipolytica. Three fungal cellulases, Talaromyces emersonii-Trichoderma reesei chimeric cellobiohydrolase I (chimeric-CBH I), T. reesei cellobiohydrolase II (CBH II), and T. reesei endoglucanase II (EG II) were expressed using three constitutive strong promoters as a single integrative expression block in a recently engineered lipid hyper-accumulating strain of Y. lipolytica (HA1). In yeast extract-peptone-dextrose (YPD) medium, the resulting cellulase co-expressing transformant YL165-1more »« less
Cited by 1
DOI: 10.3389/fmicb.2018.03276
Ameliorating the Metabolic Burden of the Co-expression of Secreted Fungal Cellulases in a High Lipid-Accumulating Yarrowia lipolytica Strain by Medium C/N Ratio and a Chemical Chaperone

Journal Article Wei, Hui ; Wang, Wei ; Alper, Hal S. ; ... - Frontiers in Microbiology

Yarrowia lipolytica, known to accumulate lipids intracellularly, lacks the cellulolytic enzymes needed to break down solid biomass directly. This study aimed to evaluate the potential metabolic burden of expressing core cellulolytic enzymes in an engineered high lipid-accumulating strain of Y. lipolytica. Three fungal cellulases, Talaromyces emersonii-Trichoderma reesei chimeric cellobiohydrolase I (chimeric-CBH I), T. reesei cellobiohydrolase II (CBH II), and T. reesei endoglucanase II (EG II) were expressed using three constitutive strong promoters as a single integrative expression block in a recently engineered lipid hyper-accumulating strain of Y. lipolytica (HA1). In yeast extract-peptone-dextrose (YPD) medium, the resulting cellulase co-expressing transformant YL165-1more »« less
Cited by 1
DOI: 10.3389/fmicb.2018.03276

Full Text Available
Fatty alcohol production in Lipomyces starkeyi and Yarrowia lipolytica

Journal Article Wang, Wei ; Wei, Hui ; Knoshaug, Eric ; ... - Biotechnology for Biofuels

Current biological pathways to produce biofuel intermediates amenable to separations and catalytic upgrading to hydrocarbon fuels are not cost effective. Previously, oleaginous yeasts have been investigated primarily for lipid production. However, yeasts store neutral lipids intracellularly making recovery difficult and expensive. In addition, once recovered from the cells, lipids are difficult to blend directly with the existing fuels without upgrading. We have, therefore, begun to investigate secreted fatty acid-derived products which can be easily recovered and upgraded to fuels. In this study, we successfully demonstrate the production of fatty alcohols by the oleaginous yeasts, Yarrowia lipolytica and Lipomyces starkeyi, throughmore »« less
Cited by 10
DOI: 10.1186/s13068-016-0647-2

Full Text Available
Functional overexpression and characterization of lipogenesis-related genes in the oleaginous yeast Yarrowia lipolytica

Journal Article Silverman, Andrew M. ; Qiao, Kangjian ; Xu, Peng ; ... - Applied Microbiology and Biotechnology

Single cell oil (SCO) is an attractive energy source due to scalability, utilization of low-cost renewable feedstocks, and type of product(s) made. Engineering strains capable of producing high lipid titers and yields is crucial to the economic viability of these processes. However, lipid synthesis in cells is a complex phenomenon subject to multiple layers of regulation, making gene target identification a challenging task. In this study, we aimed to identify genes in the oleaginous yeast Yarrowia lipolytica whose overexpression enhances lipid production by this organism. Here, to this end, we examined the effect of the overexpression of a set ofmore »« less
Cited by 28
DOI: 10.1007/s00253-016-7376-0

Full Text Available
Expression and secretion of fungal endoglucanase II and chimeric cellobiohydrolase I in the oleaginous yeast Lipomyces starkeyi

Journal Article Xu, Qi ; Knoshaug, Eric P. ; Wang, Wei ; ... - Microbial Cell Factories

Lipomyces starkeyi is one of the leading lipid-producing microorganisms reported to date; its genetic transformation was only recently reported. Our aim is to engineer L. starkeyi to serve in consolidated bioprocessing (CBP) to produce lipid or fatty acid-related biofuels directly from abundant and low-cost lignocellulosic substrates. To evaluate L. starkeyi in this role, we first conducted a genome analysis, which revealed the absence of key endo- and exocellulases in this yeast, prompting us to select and screen four signal peptides for their suitability for the overexpression and secretion of cellulase genes. To compensate for the cellulase deficiency, we chose twomore »« less
Cited by 4
DOI: 10.1186/s12934-017-0742-5

Full Text Available

Similar Records