Title: pH selectively regulates citric acid and lipid production in Yarrowia lipolytica W29 during nitrogen-limited growth on glucose

Journal Article · · Journal of Biotechnology
 [1];  [2];  [2];  [2]
  1. Univ. of Illinois, Urbana-Champaign, IL (United States). DOE Center for Advanced Bioenergy and Bioproducts Innovation, Carl R. Woese Inst. of Genomic Biology, Dept. of Chemical and Biomolecular Engineering; CABBI
  2. Univ. of Illinois, Urbana-Champaign, IL (United States). DOE Center for Advanced Bioenergy and Bioproducts Innovation, Carl R. Woese Inst. of Genomic Biology, Dept. of Chemical and Biomolecular Engineering
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Yarrowia lipolytica has been used to produce both citric acid and lipid-based bioproducts at high titers. In this study, we found that pH differentially affects citric acid and lipid production in Y. lipolytica W29, with citric acid production enhanced at more neutral pH’s and lipid production enhanced at more acid pH’s. To determine the mechanism governing this pH-dependent switch between citric acid and lipid production, we profiled gene expression at different pH’s and found that the relative expression of multiple transporters is increased at neutral pH. These results suggest that this pH-dependent switch is mediated at the level of citric acid transport rather than changes in the expression of the enzymes involved in citric acid and lipid metabolism. In further support of this mechanism, thermodynamic calculations suggest that citric acid secretion is more energetically favorable at neutral pH’s, assuming the fully protonated acid is the substrate for secretion. Finally, collectively, these results provide new insights regarding citric acid and lipid production in Y. lipolytica and may offer new strategies for metabolic engineering and process design.

Research Organization:
Center for Advanced Bioenergy and Bioproducts Innovation (CABBI), Urbana, IL (United States); Center for advanced Bioenergy and Bioproducts Innovation (CABBI), Urbana, IL (United States)
Sponsoring Organization:
USDOE Office of Science (SC), Biological and Environmental Research (BER); USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
Grant/Contract Number:
SC0018420
OSTI ID:
1484862
Journal Information:
Journal of Biotechnology, Journal Name: Journal of Biotechnology Vol. 290; ISSN 0168-1656
Publisher:
ElsevierCopyright Statement
Country of Publication:
United States
Language:
English

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Corrigendum: Multi-Omics Analysis of Fatty Alcohol Production in Engineered Yeasts Saccharomyces cerevisiae and Yarrowia lipolytica journal January 2021
Microbial production of (2 R ,3 S )-isocitric acid: state of the arts and prospects journal November 2019
Multi-Omics Analysis of Fatty Alcohol Production in Engineered Yeasts Saccharomyces cerevisiae and Yarrowia lipolytica text January 2019
Multi-Omics Analysis of Fatty Alcohol Production in Engineered Yeasts Saccharomyces cerevisiae and Yarrowia lipolytica journal August 2019
Corrigendum: Multi-Omics Analysis of Fatty Alcohol Production in Engineered Yeasts Saccharomyces cerevisiae and Yarrowia lipolytica journal January 2021

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59 BASIC BIOLOGICAL SCIENCES
Citric acid
Lipids
Oleaginous yeast
RNAseq
Transporter
Yarrowia lipolytica
citric acid
lipids
oleaginous yeast
transporter